高転写状態解明に向けた空間マルチオミクス解析技術の開発

• Project Area: Ensuring integrity in gametogenesis
• Project/Area Number: 21H00232
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyushu University
• Principal Investigator: 大川 恭行 九州大学, 生体防御医学研究所, 教授 (80448430)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000); Fiscal Year 2022: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2021: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 高転写状態 / 原始生殖細 / 細胞分化制御 / トランスクリプトーム / エピゲノム / 原始生殖細胞 / 始原生殖細胞

Outline of Research at the Start

幹細胞は、特定のヒストンバリアントを取り込むことで特異的なクロマチン構造を形成し、通常の体細胞より全遺伝子の転写活性状態が極めて高い高転写状態にある。中でも成熟卵母細胞、精原細胞の胚性前駆細胞である始原生殖細胞の解析は最も先駆的に行われている系の一つである。高転写状態は、初期発生時の爆発的な細胞増殖や多系統への分化に不可欠と考えられているが、その分子機序はいまだ不明である。高転写状態がもたらす遺伝子発現の“量的”制御の解明にはクロマチン構造の形成による転写量制御から、タンパク質の発現まで包括的に理解する必要がある。そこで、本研究では、新規技術開発を行う。

Outline of Annual Research Achievements

私たちはこれまで免疫染色をベースに、細胞・組織を非破壊的に標的タンパク質を可視化し、同時にゲノム上の位置を決定するChILSeqを開発した。ChILSeq では抗体反応領域周辺のゲノムDNAにTn5トランスポザーゼを用いて選択的にT7RNA ポリメラーゼプロモータ配列を挿入し、T7RNAポリメラーゼの添加により強制的に転写させることでゲノム情報をRNAとして獲得する。ChIL反応により転写されたRNA はゲノムから遊離しないため、細胞の形態や組織内の位置情報を保持した状態でsmFISH により検出可能である。そこで、本研究ではChILsmFISH により、NGSなしでのイメージングのみでハイスループット且つ1細胞レベルの高解像度エピゲノム解析技術が樹立し、エピゲノムとトランスクリプトームの同時測定技術の樹立を試みた。加えて、高転写状態解析に必要となるタンパク質の発現情報の取得を進めた。本研究では、プロテオミクスまで含めたマルチオミクス技術を開発にとりくんだ。プロテオミクスの適用の為新たに開発した連続蛍光免疫染色法（seqImmuno-Fluo:以下seqIF）の実装を行い、200種を超えるプローブの作成と検証を行い、ヒト検体を用いた実データ取得を進めた。更に、これら解析を行うための自動化デバイスの開発に取り組み、共焦点レーザー顕微鏡下でデータを自動的に取得し、反応を行う新たなデバイス開発に成功した。現在論文投稿準備中である。また、トランスクリプトーム技術seqFISH+との統合を進めた。

Research Progress Status

令和4年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和4年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Design and implementation of a hybrid cloud system for large-scale human genomic research. (2023)
  • Author(s): Masao Nagasaki, Yayoi Sekiya, Akihiro Asakura, Ryo Teraoka, Ryoko Otokozawa, Hiroki Hashimoto, Takahisa Kawaguchi, Keiichiro Fukazawa, Yuichi Inadomi, Ken T Murata, Yasuyuki Ohkawa, Izumi Yamaguchi, Takamichi Mizuhara, Katsushi Tokunaga, Yuji Sekiya, Toshihiro Hanawa, Ryo Yamada, Fumihiko Matsuda.
  • Journal Title: Hum Genome Var. Volume: 10 Issue: 1 Pages: 6-6
  • DOI: 10.1038/s41439-023-00231-2
  • Peer Reviewed / Open Access
• [Journal Article] Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration. (2023)
  • Author(s): Moiseeva V, Cisneros A, Sica V, Deryagin O, Lai Y, Jung S, Andres E, An J, Segales J, Ortet L, Lukesova V, Volpe G, Benguria A, Dopazo A, Benitah SA, Urano Y, Del Sol A, Esteban MA, Ohkawa Y, Serrano AL, Perdiguero E, Munoz-Canoves P.
  • Journal Title: Nature Volume: 613 Issue: 7942 Pages: 169-178
  • DOI: 10.1038/s41586-022-05535-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity (2022)
  • Author(s): Ohishi Hiroaki、Shimada Seiru、Uchino Satoshi、Li Jieru、Sato Yuko、Shintani Manabu、Owada Hitoshi、Ohkawa Yasuyuki、Pertsinidis Alexandros、Yamamoto Takashi、Kimura Hiroshi、Ochiai Hiroshi
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 7672-7672
  • DOI: 10.1038/s41467-022-35286-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prostaglandin E2 receptor Ptger4b regulates female-specific peptidergic neurons and female sexual receptivity in medaka (2022)
  • Author(s): Fleming Thomas、Kikuchi Yukiko、Nakajo Mikoto、Tachizawa Masaya、Inazumi Tomoaki、Tsuchiya Soken、Sugimoto Yukihiko、Saito Daisuke、Suyama Mikita、Ohkawa Yasuyuki、Baba Takashi、Morohashi Ken-ichirou、Okubo Kataaki
  • Journal Title: Communications Biology Volume: 5 Issue: 1 Pages: 1215-1215
  • DOI: 10.1038/s42003-022-04195-x
  • Peer Reviewed / Open Access
• [Journal Article] Tmsb10 triggers fetal Leydig differentiation by suppressing the RAS/ERK pathway. (2022)
  • Author(s): Miki Inoue, Takashi Baba, Fumiya Takahashi, Miho Terao, Shogo Yanai, Yuichi Shima, Daisuke Saito, Kei Sugihara, Takashi Miura, Shuji Takada, Mikita Suyama, Yasuyuki Ohkawa, Ken-Ichirou Morohashi.
  • Journal Title: Commun Biol. Volume: 5 Issue: 1 Pages: 974-974
  • DOI: 10.1038/s42003-022-03941-5
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a drug-response gene in multiple myeloma through longitudinal single-cell transcriptome sequencing (2022)
  • Author(s): Masuda Toru、Haji Shojiro、Nakashima Yasuhiro、Tsuda Mariko、Kimura Daisaku、Takamatsu Akiko、Iwahashi Norifusa、Umakoshi Hironobu、Shiratsuchi Motoaki、Kikutake Chie、Suyama Mikita、Ohkawa Yasuyuki、Ogawa Yoshihiro
  • Journal Title: iScience Volume: 25 Issue: 8 Pages: 104781-104781
  • DOI: 10.1016/j.isci.2022.104781
  • Peer Reviewed / Open Access
• [Journal Article] Uterus-specific transcriptional regulation underlies eggshell pigment production in Japanese quail (2022)
  • Author(s): Ishishita Satoshi、Kitahara Shumpei、Takahashi Mayuko、Iwasaki Sakura、Tatsumoto Shoji、Hara Izumi、Kaneko Yoshiki、Kinoshita Keiji、Yamaguchi Katsushi、Harada Akihito、Ohmori Yasushige、Ohkawa Yasuyuki、Go Yasuhiro、Shigenobu Shuji、Matsuda Yoichi、Suzuki Takayuki
  • Journal Title: PLOS ONE Volume: 17 Issue: 3 Pages: 0265008-0265008
  • DOI: 10.1371/journal.pone.0265008
  • Peer Reviewed / Open Access
• [Journal Article] Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections (2022)
  • Author(s): Honda Mizuki、Kimura Ryuichi、Harada Akihito、Maehara Kazumitsu、Tanaka Kaori、Ohkawa Yasuyuki、Oki Shinya
  • Journal Title: STAR Protocols Volume: 3 Issue: 2 Pages: 101346-101346
  • DOI: 10.1016/j.xpro.2022.101346
  • Peer Reviewed / Open Access
• [Journal Article] A spinal microglia population involved in remitting and relapsing neuropathic pain (2022)
  • Author(s): Kohno Keita、Shirasaka Ryoji、Yoshihara Kohei、Mikuriya Satsuki、Tanaka Kaori、Takanami Keiko、Inoue Kazuhide、Sakamoto Hirotaka、Ohkawa Yasuyuki、Masuda Takahiro、Tsuda Makoto
  • Journal Title: Science Volume: 376 Issue: 6588 Pages: 86-90
  • DOI: 10.1126/science.abf6805
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tenogenic Induction From Induced Pluripotent Stem Cells Unveils the Trajectory Towards Tenocyte Differentiation (2022)
  • Author(s): Yoshimoto Yuki、Uezumi Akiyoshi、Ikemoto-Uezumi Madoka、Tanaka Kaori、Yu Xinyi、Kurosawa Tamaki、Yambe Shinsei、Maehara Kazumitsu、Ohkawa Yasuyuki、Sotomaru Yusuke、Shukunami Chisa
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 10 Pages: 780038-780038
  • DOI: 10.3389/fcell.2022.780038
  • Peer Reviewed / Open Access
• [Journal Article] Direct Conversion of Human Endothelial Cells Into Liver Cancer-Forming Cells Using Nonintegrative Episomal Vectors. (2022)
  • Author(s): Goya T, Horisawa K, Udono M, Ohkawa Y, Ogawa Y, Sekiya S, Suzuki A.
  • Journal Title: Hepatol Commun. Volume: - Issue: 7 Pages: 1725-1740
  • DOI: 10.1002/hep4.1911
  • Peer Reviewed / Open Access
• [Journal Article] Uhrf1 governs the proliferation and differentiation of muscle satellite cells. (2022)
  • Author(s): Sakai H, Sawada Y, Tokunaga N, Tanaka K, Nakagawa S, Sakakibara I, Ono Y, Fukada SI, Ohkawa Y, Kikugawa T, Saika T, Imai Y.
  • Journal Title: iScience. Volume: 25 Issue: 3 Pages: 103928-103928
  • DOI: 10.1016/j.isci.2022.103928
  • Peer Reviewed / Open Access
• [Journal Article] Uterus-specific transcriptional regulation underlies eggshell pigment production in Japanese quail. (2022)
  • Author(s): Ishishita S, Kitahara S, Takahashi M, Iwasaki S, Tatsumoto S, Hara I, Kaneko Y, Kinoshita K, Yamaguchi K, Harada A, Ohmori Y, Ohkawa Y, Go Y, Shigenobu S, Matsuda Y, Suzuki T.
  • Journal Title: PLoS One. Volume: 17
  • Peer Reviewed / Open Access
• [Journal Article] Live imaging of transcription sites using an elongating RNA polymerase II-specific probe. (2022)
  • Author(s): Uchino S, Ito Y, Sato Y, Handa T, Ohkawa Y, Tokunaga M, Kimura H.
  • Journal Title: J Cell Biol. Volume: 221 Issue: 2 Pages: 1-23
  • DOI: 10.1083/jcb.202104134
  • Peer Reviewed / Open Access
• [Journal Article] Unusual nucleosome formation and transcriptome influence by the histone H3mm18 variant (2021)
  • Author(s): Hirai Seiya、Tomimatsu Kosuke、Miyawaki-Kuwakado Atsuko、Takizawa Yoshimasa、Komatsu Tetsuro、Tachibana Taro、Fukushima Yutaro、Takeda Yasuko、Negishi Lumi、Kujirai Tomoya、Koyama Masako、Ohkawa Yasuyuki、Kurumizaka Hitoshi
  • Journal Title: Nucleic Acids Research Volume: 50 Issue: 1 Pages: 72-91
  • DOI: 10.1093/nar/gkab1137
  • Peer Reviewed / Open Access
• [Journal Article] Relayed signaling between mesenchymal progenitors and muscle stem cells ensures adaptive stem cell response to increased mechanical load. (2021)
  • Author(s): Kaneshige A, Kaji T, Zhang L, Saito H, Nakamura A, Kurosawa T, Ikemoto-Uezumi M, Tsujikawa K, Seno S, Hori M, Saito Y, Matozaki T, Maehara K, Ohkawa Y, Potente M, Watanabe S, Braun T, Uezumi A, Fukada SI.
  • Journal Title: Cell Stem Cell. Volume: 29 Issue: 2 Pages: 265-280
  • DOI: 10.1016/j.stem.2021.11.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recent advances in single-cell epigenomics. (2021)
  • Author(s): Harada A, Kimura H, Ohkawa Y.
  • Journal Title: Curr Opin Struct Biol. Volume: 71 Pages: 116-122
  • Peer Reviewed / Open Access
• [Journal Article] High-throughput single-cell epigenomic profiling by targeted insertion of promoters (TIP-seq). (2021)
  • Author(s): Bartlett DA, Dileep V, Handa T, Ohkawa Y, Kimura H, Henikoff S, Gilbert DM.
  • Journal Title: J Cell Biol. Volume: 220 Issue: 12
  • DOI: 10.1083/jcb.202103078
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chromatin loading of MCM hexamers is associated with di-/tri-methylation of histone H4K20 toward S phase entry. (2021)
  • Author(s): Hayashi-Takanaka Y, Hayashi Y, Hirano Y, Miyawaki-Kuwakado A, Ohkawa Y, Obuse C, Kimura H, Haraguchi T, Hiraoka Y.
  • Journal Title: Nucleic Acids Res. Volume: 49 Issue: 21 Pages: 12152-12166
  • DOI: 10.1093/nar/gkab1068
  • Peer Reviewed / Open Access
• [Journal Article] Discriminative feature of cells characterizes cell populations of interest by a small subset of genes. (2021)
  • Author(s): Fujii T, Maehara K, Fujita M, Ohkawa Y.
  • Journal Title: PLoS Comput Biol. Volume: 17 Issue: 11 Pages: e1009579-e1009579
  • DOI: 10.1371/journal.pcbi.1009579
  • Peer Reviewed / Open Access
• [Journal Article] Sex differences in metabolic pathways are regulated by Pfkfb3 and Pdk4 expression in rodent muscle. (2021)
  • Author(s): Christianto A, Baba T, Takahashi F, Inui K, Inoue M, Suyama M, Ono Y, Ohkawa Y, Morohashi KI.
  • Journal Title: Commun Biol. Volume: 4 Issue: 1 Pages: 1264-1264
  • DOI: 10.1038/s42003-021-02790-y
  • Peer Reviewed / Open Access
• [Journal Article] Neural stem/precursor cells dynamically change their epigenetic landscape to differentially respond to BMP signaling for fate switching during brain development. (2021)
  • Author(s): Katada S, Takouda J, Nakagawa T, Honda M, Igarashi K, Imamura T, Ohkawa Y, Sato S, Kurumizaka H, Nakashima K.
  • Journal Title: Genes Dev. Volume: 35 Pages: 1431-1444
  • Peer Reviewed / Open Access
• [Journal Article] Modeling population size independent tissue epigenomes by ChIL-seq with single thin sections. (2021)
  • Author(s): Maehara K, Tomimatsu K, Harada A, Tanaka K, Sato S, Fukuoka M, Okada S, Handa T, Kurumizaka H, Saitoh N, Kimura H, Ohkawa Y.
  • Journal Title: Mol Syst Biol. Volume: 17
  • Peer Reviewed / Open Access
• [Journal Article] Genome-wide analysis of chromatin structure changes upon MyoD binding in proliferative myoblasts during the cell cycle (2021)
  • Author(s): Wu Qianmei、Fujii Takeru、Harada Akihito、Tomimatsu Kosuke、Miyawaki-Kuwakado Atsuko、Fujita Masatoshi、Maehara Kazumitsu、Ohkawa Yasuyuki
  • Journal Title: The Journal of Biochemistry Volume: - Issue: 6 Pages: 653-661
  • DOI: 10.1093/jb/mvab001
  • NAID: 40022687719
  • Peer Reviewed / Open Access
• [Journal Article] Integrated Analysis of Transcriptome and Histone Modifications in Granulosa Cells During Ovulation in Female Mice (2021)
  • Author(s): Shirafuta Yuichiro、Tamura Isao、Ohkawa Yasuyuki、Maekawa Ryo、Doi-Tanaka Yumiko、Takagi Haruka、Mihara Yumiko、Shinagawa Masahiro、Taketani Toshiaki、Sato Shun、Tamura Hiroshi、Sugino Norihiro
  • Journal Title: Endocrinology Volume: 162 Issue: 9 Pages: 1-17
  • DOI: 10.1210/endocr/bqab128
  • Peer Reviewed / Open Access
• [Journal Article] An extensive and dynamic trans-omic network illustrating prominent regulatory mechanisms in response to insulin in the liver. (2021)
  • Author(s): Matsuzaki F, Uda S, Yamauchi Y, Matsumoto M, Soga T, Maehara K, Ohkawa Y, Nakayama KI, Kuroda S, Kubota H.
  • Journal Title: Cell Rep. Volume: 36 Pages: 109569-109569
  • Peer Reviewed / Open Access
• [Journal Article] Targeted inhibition of EPAS1-driven IL-31 production by a small-molecule compound. (2021)
  • Author(s): Kamikaseda Y, Uruno T, Kunimura K, Harada A, Saiki K, Oisaki K, Sakata D, Nakahara T, Kido-Nakahara M, Kanai M, Nakamura S, Ohkawa Y, Furue M, Fukui Y.
  • Journal Title: J Allergy Clin Immunol. Volume: 148 Issue: 2 Pages: 633-638
  • DOI: 10.1016/j.jaci.2021.03.029
  • Peer Reviewed / Open Access
• [Journal Article] High-depth spatial transcriptome analysis by photo-isolation chemistry. (2021)
  • Author(s): Honda M, Oki S, Kimura R, Harada A, Maehara K, Tanaka K, Meno C, Ohkawa Y.
  • Journal Title: Nat Commun. Volume: 12 Issue: 1 Pages: 4416-4416
  • DOI: 10.1038/s41467-021-24691-8
  • NAID: 120007141968
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptome analysis of gene expression changes upon enzymatic dissociation in skeletal myoblasts. (2021)
  • Author(s): Miyawaki-Kuwakado A, Wu Q, Harada A, Tomimatsu K, Fujii T, Maehara K, Ohkawa Y.
  • Journal Title: Genes Cells. Volume: 26 Issue: 7 Pages: 530-540
  • DOI: 10.1111/gtc.12870
  • Peer Reviewed / Open Access
• [Journal Article] Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle. (2021)
  • Author(s): Yoshioka K, Nagahisa H, Miura F, Araki H, Kamei Y, Kitajima Y, Seko D, Nogami J, Tsuchiya Y, Okazaki N, Yonekura A, Ohba S, Sumita Y, Chiba K, Ito K, Asahina I, Ogawa Y, Ito T, Ohkawa Y, Ono Y.
  • Journal Title: Sci Adv. Volume: 7 Issue: 24
  • DOI: 10.1126/sciadv.abd7924
  • Peer Reviewed / Open Access
• [Journal Article] Totipotency of mouse zygotes extends to single blastomeres of embryos at the four-cell stage. (2021)
  • Author(s): Maemura M, Taketsuru H, Nakajima Y, Shao R, Kakihara A, Nogami J, Ohkawa Y, Tsukada YI.
  • Journal Title: Sci Rep. Volume: 11 Issue: 1 Pages: 11167-11167
  • DOI: 10.1038/s41598-021-90653-1
  • Peer Reviewed / Open Access
• [Journal Article] Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay (2021)
  • Author(s): Hiroaki Tachiwana, Mariko Dacher, Kazumitsu Maehara, Akihito Harada, Yosuke Seto, Ryohei Katayama, Yasuyuki Ohkawa, Hiroshi Kimura, Hitoshi Kurumizaka, Noriko Saitoh
  • Journal Title: eLife Volume: 10
  • DOI: 10.7554/elife.66290
  • Peer Reviewed / Open Access
• [Presentation] 単一細胞マルチオミクスによる骨格筋分化制御機構の解明 (2023)
  • Author(s): 大川恭行
  • Organizer: 第6回運動器と健康研究会
  • Invited
• [Presentation] Spatial multi-omics for understanding chromatin dynamics (2023)
  • Author(s): 大川恭行
  • Organizer: 2023年度高深度オミクスシンポジウム
  • Invited
• [Presentation] データから変化を取り出す新たな単一細胞解析技術 (2023)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: 【富士フイルム】講演
  • Invited
• [Presentation] Single-cell multi-targeted chromatin integration labeling technology for understanding chromatin dynamics (2022)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: CSHA meeting on Integrative Epigenetics in Plants
  • Int'l Joint Research / Invited
• [Presentation] 空間オミクス技術によるクロマチン構造制御メカニズムの解明 (2022)
  • Author(s): 大川恭行
  • Organizer: 第95回日本生化学会大会
  • Invited
• [Presentation] 細胞恒常性を担うシグナルと遺伝子発現の制御 (2022)
  • Author(s): 大川恭行
  • Organizer: 第16回日本臨床ストレス応答学会
  • Invited
• [Presentation] 高深度解析による全ゲノムレベルの単一細胞オミクスへの挑戦 (2022)
  • Author(s): 大川恭行
  • Organizer: NGS EXPO 2022
  • Invited
• [Presentation] 単一細胞軌跡追跡によるクロマチンダイナミクスの解明 (2022)
  • Author(s): 大川恭行
  • Organizer: 2022年度国立遺伝学研究所クロマチン研究会
  • Invited
• [Presentation] Chromatin regulation during skeletal muscle regeneration (2022)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: 第17回生命医科学研究所ネットワーク国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] 骨格筋分化のクロマチンダイナミクス (2022)
  • Author(s): 大川恭行
  • Organizer: 日本遺伝学会第94回大会
  • Invited
• [Presentation] トランスクリプトミクスで迫る細胞分化能 (2022)
  • Author(s): 大川恭行
  • Organizer: 大阪大学産業科学研究所セミナー
  • Invited
• [Presentation] Spatial multi-omics for understanding gene expression regulated by cell-cell interaction. (2022)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: Japan-UK Regulation through Chromatin Conference
  • Int'l Joint Research / Invited
• [Presentation] 空間オミクスによる骨格筋組織老化機序の解明 (2022)
  • Author(s): 大川恭行
  • Organizer: 第8回日本筋学会学術集会
  • Invited
• [Presentation] クロマチン解析のための新技術開発の現状と今後の展開 (2022)
  • Author(s): 大川恭行
  • Organizer: 第15回日本エピジェネティクス研究会年会
  • Invited
• [Presentation] 骨格筋再性能を制御するクロマチン構造等その破綻 (2021)
  • Author(s): 大川恭行
  • Organizer: 第94回日本生化学会大会
  • Invited
• [Presentation] 空間マルチオミクスの展開～エピゲノム, トランスクリプトームからプロテオームの空間計測の試み～ (2021)
  • Author(s): 大川恭行
  • Organizer: がん研セミナー
  • Invited
• [Presentation] Identification and Analysis of Minor Histone Variants (2021)
  • Author(s): Yasuyuki Ohkawa
  • Organizer: EMBO Workshop Physiology and function of histone variants
• [Book] Journal of Japanese Biochemical Society (2021)
  • Author(s): 原田 哲仁，大川 恭行
  • Publisher: 日本生化学会
• [Book] 実験医学2021年9月号 (2021)
  • Author(s): 沖 真弥、大川 恭行
  • Total Pages: 131
  • Publisher: 羊土社
  • ISBN: 9784758125475
• [Book] 遺伝子医学MOOK36 (2021)
  • Author(s): 富松航佑, 大川 恭行
  • Total Pages: 218
  • Publisher: 株式会社メディカルドゥ
• [Remarks] 九州大学 トランスクリプトミクス分野ホームページ
  • URL: https://tx.bioreg.kyushu-u.ac.jp/