“パイオニア”動物でひもとく海から淡水、陸上への進出をもたらしたシンギュラリティ

• Project Area: Singularity biology
• Project/Area Number: 21H00428
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Okayama University
• Principal Investigator: 坂本 竜哉 岡山大学, 自然科学学域, 教授 (10294480)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2022: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2021: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 進化 / バソプレシン

Outline of Research at the Start

体内“細胞外液”とその調節能を獲得したパイオニア、扁形動物の原始“抗利尿ホルモン系—腎臓”を橋頭保に、海から新しい水・塩分環境への動物の不連続な進出をもたらしたシンギュラリティを検証する。
①プラチトシン系の発現様式の解明とシンギュラリティ細胞である可能性の究明
②シンギュラリティとしてのプラチトシン系－原腎管の細胞・分子基盤の解明
③プラチトシン系－原腎管の環境進出におけるシンギュラリティ機能の個体レベルの解明

Outline of Annual Research Achievements

“プラナリア”など扁形動物は、基盤的な左右相称動物で、初めて本格的に新しい水・塩分環境に進出した。我々は、広塩性のヒラムシにおいて、抗利尿ホルモン系の祖先型を同定し、プラチトシン系と命名した。そして、抗脱水に必須であることも見出した。『抗利尿ホルモン系による“腎臓” i.e., 体液の調節』が扁形動物まで遡れ、新環境進出のシンギュラリティの可能性がある。また、プラチトシンは脳神経節の2対のニューロンのみで産生されることも発見した（シンギュラリティ細胞？）。この2対の細胞は、脊椎動物の2群に分化している数千の抗利尿ホルモン産生細胞（脳内で作用するもの・ホルモンを血中に放出し全身に作用するもの）に対応する原型の可能性がある。これらは前左右相称動物のゲノム等には見出せない。神経中枢化がはじまり“細胞外液”を獲得した扁形動物の神経ペプチド群は、神経シナプス系からの内分泌系/液性調節の誕生：神経内分泌～脳による生体制御系のシンギュラリティとも考えられる。
そこで、SPring-8での3Dマイクロ-CT法により、脳神経節を3D再構築した。前口動物（キノコ体）・後口動物（視交叉）双方の特徴をもち“原始脳”である可能性が示された。さらに、この脳神経節から、進化的に普遍的な神経ペプチドの祖先群として、生殖腺刺激ホルモン放出ホルモン（GnRH）等も発見した。現在、それらのはたらきと、１細胞/空間トランスクリプトームにより、そのニューロン回路/分子基盤を検討し、データ駆動型の発展も目論んでいる。

Research Progress Status

令和4年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和4年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] ソルボンヌ大/ロスコフ研(フランス)
• [Int'l Joint Research] Vertex Pharmaceuticals (Europe) Ltd./University of Oxford(英国)
• [Int'l Joint Research] University of Auckland/University of Otago(ニュージーランド)
• [Int'l Joint Research] University of California, Davis(米国)
• [Journal Article] Evolutionary differentiation of androgen receptor is responsible for sexual characteristic development in a teleost fish (2023)
  • Author(s): Y. Ogino, S. Ansai, E. Watanabe, M. Yasugi, Y. Katayama, H. Sakamoto, K. Okamoto, K. Okubo, Y. Yamamoto, I. Hara, T. Yamazaki, A. Kato, Y. Kamei, K. Naruse, K. Ohta, H. Ogino, T. Sakamoto, S. Miyagawa, T. Sato, G. Yamada, M. Baker & T. Iguchi
  • Journal Title: Nature communications Volume: 14 Issue: 1 Pages: 1428-1428
  • DOI: 10.1038/s41467-023-37026-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chronic corticosterone exposure evokes itch hypersensitivity and sexual dysfunction in male rats: relationship between the two distinct gastrin-releasing peptide systems in the spinal cord (2023)
  • Author(s): Takanami Keiko、Morishita Makoto、Sakamoto Tatsuya、Sakamoto Hirotaka
  • Journal Title: General and Comparative Endocrinology Volume: - Pages: 114289-114289
  • DOI: 10.1016/j.ygcen.2023.114289
  • Peer Reviewed / Open Access
• [Journal Article] Behavioural osmoregulation during land invasion in fish: Prandial drinking and wetting of the dry skin (2022)
  • Author(s): Katayama Yukitoshi、Tsukada Takehiro、Hyodo Susumu、Sakamoto Hirotaka、Sakamoto Tatsuya
  • Journal Title: PLOS ONE Volume: 17 Issue: 12 Pages: 1-14
  • DOI: 10.1371/journal.pone.0277968
  • Peer Reviewed / Open Access
• [Journal Article] De novo transcriptome analysis of the centrohelid <i>Raphidocystis contractilis</i> to identify genes involved in microtubule‐based motility (2022)
  • Author(s): Ikeda Risa、Sakagami Tosuke、Hamada Mayuko、Sakamoto Tatsuya、Hatabu Toshimitsu、Saito Noboru、Ando Motonori
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 70 Issue: 2
  • DOI: 10.1111/jeu.12955
  • Peer Reviewed / Open Access
• [Journal Article] Squalomix: shark and ray genome analysis consortium and its data sharing platform (2022)
  • Author(s): Nishimura Osamu、Sakamoto Tatsuya、Semba Yasuko、Yamauchi Shinya、Yamada Kazuyuki、Nishida Kiyonori、Kiyatake Itsuki、Sato Keiichi、Hyodo Susumu、Kadota Mitsutaka、Uno Yoshinobu、Kuraku Shigehiro
  • Journal Title: F1000Research Volume: 11 Pages: 1077-1077
  • DOI: 10.12688/f1000research.123591.1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Footedness for scratching itchy eyes in rodents (2022)
  • Author(s): Katayama Yukitoshi、Miura Ayane、Sakamoto Tatsuya、Takanami Keiko、Sakamoto Hirotaka
  • Journal Title: Proceedings of the Royal Society B: Biological Sciences Volume: 289 Issue: 1985 Pages: 1-9
  • DOI: 10.1098/rspb.2022.1126
  • Peer Reviewed / Open Access
• [Journal Article] Molecular and morphological investigations on the renal mechanisms enabling euryhalinity of red stingray Hemitrygon akajei (2022)
  • Author(s): Aburatani Naotaka、Takagi Wataru、Wong Marty Kwok-Shing、Kuraku Shigehiro、Tanegashima Chiharu、Kadota Mitsutaka、Saito Kazuhiro、Godo Waichiro、Sakamoto Tatsuya、Hyodo Susumu
  • Journal Title: Frontiers in Physiology Volume: 13
  • DOI: 10.3389/fphys.2022.953665
  • Peer Reviewed / Open Access
• [Journal Article] Lattice-patterned collagen fibers and their dynamics in axolotl skin regeneration (2022)
  • Author(s): Kashimoto Rena、Furukawa Saya、Yamamoto Sakiya、Kamei Yasuhiro、Sakamoto Joe、Nonaka Shigenori、Watanabe Tomonobu M.、Sakamoto Tatsuya、Sakamoto Hirotaka、Satoh Akira
  • Journal Title: iScience Volume: 25 Issue: 7 Pages: 104524-104524
  • DOI: 10.1016/j.isci.2022.104524
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of the expression of gastrin‐releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans (2022)
  • Author(s): Takanami Keiko、Oti Takumi、Kobayashi Yasuhisa、Hasegawa Koki、Ito Takashi、Tsutsui Naoaki、Ueda Yasumasa、Carstens Earl、Sakamoto Tatsuya、Sakamoto Hirotaka
  • Journal Title: Journal of Comparative Neurology Volume: 530 Issue: 16 Pages: 2804-2819
  • DOI: 10.1002/cne.25376
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Vasopressin-oxytocin?type signaling is ancient and has a conserved water homeostasis role in euryhaline marine planarians (2022)
  • Author(s): Kobayashi Aoshi、Hamada Mayuko、Yoshida Masa-aki、Kobayashi Yasuhisa、Tsutsui Naoaki、Sekiguchi Toshio、Matsukawa Yuta、Maejima Sho、Gingell Joseph J.、Sekiguchi Shoko、Hamamoto Ayumu、Hay Debbie L.、Morris John F.、Sakamoto Tatsuya、Sakamoto Hirotaka
  • Journal Title: Science Advances Volume: 8 Issue: 9 Pages: 0311-0311
  • DOI: 10.1126/sciadv.abk0331
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Observing Phylum-Level Metazoan Diversity by Environmental DNA Analysis at the Ushimado Area in the Seto Inland Sea (2022)
  • Author(s): Kawashima Takeshi、Yoshida Masa-aki、Miyazawa Hideyuki、Nakano Hiroaki、Nakano Natumi、Sakamoto Tatsuya、Hamada Mayuko
  • Journal Title: Zoological Science Volume: 39 Issue: 1 Pages: 157-165
  • DOI: 10.2108/zs210073
  • NAID: 210000186961
  • Peer Reviewed / Open Access
• [Journal Article] Estrogens influence female itch sensitivity via the spinal gastrin-releasing peptide receptor neurons (2021)
  • Author(s): Keiko Takanami, Daisuke Uta, Ken Ichi Matsuda, Mitsuhiro Kawata, Earl Carstens, Tatsuya Sakamoto, Hirotaka Sakamoto
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 118 Issue: 31
  • DOI: 10.1073/pnas.2103536118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus (2021)
  • Author(s): Hirooka Asuka、Hamada Mayuko、Fujiyama Daiki、Takanami Keiko、Kobayashi Yasuhisa、Oti Takumi、Katayama Yukitoshi、Sakamoto Tatsuya、Sakamoto Hirotaka
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 13315-13315
  • DOI: 10.1038/s41598-021-92528-x
  • NAID: 120007120048
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Oxytocin Influences Male Sexual Activity via Non-synaptic Axonal Release in the Spinal Cord (2021)
  • Author(s): Oti Takumi、Satoh Keita、Uta Daisuke、Nagafuchi Junta、Tateishi Sayaka、Ueda Ryota、Takanami Keiko、Young Larry J.、Galione Antony、Morris John F.、Sakamoto Tatsuya、Sakamoto Hirotaka
  • Journal Title: Current Biology Volume: 31 Issue: 1 Pages: 103-114
  • DOI: 10.1016/j.cub.2020.09.089
  • NAID: 120007168224
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at ?25 °C for Several Years (2021)
  • Author(s): Otubo Akito、Maejima Sho、Oti Takumi、Satoh Keita、Ueda Yasumasa、Morris John F.、Sakamoto Tatsuya、Sakamoto Hirotaka
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 17 Pages: 9180-9180
  • DOI: 10.3390/ijms22179180
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sexual Experience Induces the Expression of Gastrin-Releasing Peptide and Oxytocin Receptors in the Spinal Ejaculation Generator in Rats (2021)
  • Author(s): Oti Takumi、Ueda Ryota、Kumagai Ryoko、Nagafuchi Junta、Ito Takashi、Sakamoto Tatsuya、Kondo Yasuhiko、Sakamoto Hirotaka
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 19 Pages: 10362-10362
  • DOI: 10.3390/ijms221910362
  • Peer Reviewed / Open Access
• [Journal Article] Systemic effects of oxytocin on male sexual activity via the spinal ejaculation generator in rats (2021)
  • Author(s): Oti Takumi、Sakamoto Tatsuya、Sakamoto Hirotaka
  • Journal Title: Communicative & Integrative Biology Volume: 14 Issue: 1 Pages: 55-60
  • DOI: 10.1080/19420889.2021.1902056
  • Peer Reviewed / Open Access
• [Journal Article] In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats (2021)
  • Author(s): Uta Daisuke、Oti Takumi、Sakamoto Tatsuya、Sakamoto Hirotaka
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 7 Pages: 3400-3400
  • DOI: 10.3390/ijms22073400
  • NAID: 120007029875
  • Peer Reviewed / Open Access
• [Presentation] Itch and its Molecular/Functional Evolution (2022)
  • Author(s): Takanami Keiko
  • Organizer: CECE&ISFE 2022
  • Int'l Joint Research / Invited
• [Presentation] Ushimado Marine Institute Okayama University (2022)
  • Author(s): Sakamoto Tatsuya
  • Organizer: I-MaC program @Roscoff
  • Int'l Joint Research / Invited
• [Presentation] Molecular evolution and functional conservation of itch perception and transmission (2022)
  • Author(s): Keiko Takanami
  • Organizer: Joint Conference of the European Society for Comparative Endocrinology and of the International Society for Fish Endocrinology
  • Int'l Joint Research / Invited
• [Book] Handbook of Hormones: Growth hormone/prolactin family (2021)
  • Author(s): Yamaguchi Yoko、Sakamoto Tatsuya
  • Total Pages: 2
  • Publisher: Academic Press
  • ISBN: 9780128206492
• [Book] Handbook of Hormones: Prolactin (2021)
  • Author(s): Yamaguchi Yoko、Sakamoto Tatsuya
  • Total Pages: 3
  • Publisher: Academic Press
  • ISBN: 9780128206492
• [Book] Handbook of Hormones: Prolactin-releasing peptide (2021)
  • Author(s): Tetsuya Tachibana、Tatsuya Sakamoto
  • Total Pages: 4
  • Publisher: Academic Press