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顕微鏡-電流同時計測による光合成膜タンパク質超分子集合系の機能解明

Publicly Offered Research

Project AreaMolecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process
Project/Area Number 22018011
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionNagoya Institute of Technology

Principal Investigator

出羽 毅久  名古屋工業大学, 工学研究科, 准教授 (70335082)

Co-Investigator(Kenkyū-buntansha) 水野 稔久  名古屋工業大学, 工学研究科, 助教 (90345950)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2011: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2010: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords光合成 / 膜タンパク質 / 脂質二分子膜 / 直接観察 / 組織化 / 原子間力顕微鏡
Research Abstract

光合成膜中での初期過程をになう膜タンパク質複合体(アンテナ複合体(LH2)および反応中心-アンテナ複合体(LH1-RC))が形成する超分子集合系の形態と機能(エネルギー捕集・電荷分離)との相関を明らかにすることができれば、光エネルギーの利用ためのデバイス化に重要な知見を得ることができる。23年度では、(1)基板上に固定化したLH2の分光学的手法(VSFG/ESFG:田原分光グループ(理研))による分子配向の観察、(2)脂質二分子膜中に再構成した光合成膜タンパク質(LH1-RC/LH2)の分子レベルでの集合状態の解析と、集合状態と機能(エネルギー移動)の相関にについて研究を実施した。(1)では、システインを有するミュータントLH2を作成し、その固体基板での分子配向を評価することに成功した。(2)では、異なる組成の脂質を用い、分散・集合状態の異なるLH2/LH1-RC再構成膜を作成し、集合状態をAFMにより分子解像度で観察することに成功した。蛍光分光測定により、LH2間およびLH2/LH1-RC間のエネルギー移動を評価した結果、膜中で密にパッキングしたLH2間において顕著なエネルギー移動が認められ、LH2/LH1-RCの不均一分散系においても均一分散系と同等のエネルギー移動が観察された。さらに電流計測AFMによる顕微鏡-電気化学同時計測をLH1-RC集合体について行った結果、LH1-RC分子はAu-mica上で一定の方向を向いて配列していることが分かった。また、反応中心複合体(RC)の電子移動の方向に沿ったトンネル電流が観測され、整流特性が確認できた。これにより、分子レベルでの集合状態の構造-機能相関を調べる有用な手法の手がかりを得ることができた。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

本年度計画の実験をすべて遂行することができ、それぞれに対して明確な結果および次の展開につながる知見が得られた。

Strategy for Future Research Activity

基板上に固定化したLH2の分光学的手法による分子配向観察において、部分的に予期せぬ配向を示すデータが得られていることから、LH2の化学修飾による基板への固定化を行い、分子配向の完全制御を達成する。
顕微鏡-電流同時計測によるLH1-RCの電流計測において、光照射下における微弱な光電流を検出するため、光照射系および検出系の工夫により、分子レベルでの電流計測を進める。

Report

(2 results)
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (81 results)

All 2012 2011 2010

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (64 results) Book (1 results)

  • [Journal Article] Immobilization and Photocurrent Activity of a Light-Harvesting Antenna Complex II, LHCII Isolated from a Plant on Electrodes2012

    • Author(s)
      M. Nagata, M. Amano, T. Joke, K. Fuji, A. Okuda, M. Kondo, S. Ishigure, T. Dewa, K. Iida, F. Secundo, Y. Amao, H. Hashimoto, M. Nango
    • Journal Title

      ACS Macro Lett.

      Volume: 1 Issue: 2 Pages: 296-299

    • DOI

      10.1021/mz200163e

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photocurrent and Electronic Activities of Oriented-His-tagged PhotosyntheticLight-Harvesting/Reaction Centre Core Complexes Assembled onto a Gold Electrode2012

    • Author(s)
      Masaharu Kondo, Kouji Iida, Takehisa Dewa, Hirofumi Tanaka, Takuji Ogawa, SakikoNagashima, Kenji V. P. Nagashima, Keizo Shimada, Hideki Hashimoto, Alastair T. Gardiner, Richard J. Cogdell, and Mamoru Nango
    • Journal Title

      Biomacromolecules

      Volume: 13 Issue: 2 Pages: 432-438

    • DOI

      10.1021/bm201457s

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two-Dimensional Molecular Assembly of Bacteriochlorophyll a Derivatives Using Synthetic Poly(Ethylene Glycol)-Linked Light-Harvesting Model Polypeptides on a Gold Electrode Modified with Supported Lipid Bilayers2012

    • Author(s)
      T. Ochiai, M. Nagata, K. Shimoyama, T. Kato, T. Asaoka, M. Kondo, T. Dewa, K. Yamashita, A. Kashiwada, S. Futaki, H. Hashimoto, M. Nango
    • Journal Title

      ACS Macro Lett.

      Volume: 1 Issue: 1 Pages: 28-22

    • DOI

      10.1021/mz200048m

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of Rhodobacter sphaeroides PufX-bearing maltose-binding protein and its effect on the stability of reconstituted light-harvesting core antenna complex2012

    • Author(s)
      S. Sakai, A. Hiro, M. Kondo, T. Mizuno, T. Tanaka, T. Dewa, M. Nango
    • Journal Title

      Photosynthesis Res.

      Volume: 111 Issue: 1-2 Pages: 63-69

    • DOI

      10.1007/s11120-011-9673-x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction and Structural Analysis of Tethered Lipid Bilayer Containing Photosynthetic Antenna Proteins for Functional Analysis2012

    • Author(s)
      Ayumi Sumino
    • Journal Title

      Biomacromolecules

      Volume: 12 Issue: 7 Pages: 2850-2858

    • DOI

      10.1021/bm200585y

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution and Organization of Photosynthetic Antenna Protein Complex Bearing Functional Hydrophilic Domains2011

    • Author(s)
      Shunsuke Sakai
    • Journal Title

      Chemistry Letters

      Volume: 40 Issue: 11 Pages: 1280-1282

    • DOI

      10.1246/cl.2011.1280

    • NAID

      130004426094

    • ISSN
      0366-7022, 1348-0715
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single-protein study of photoresistance of pigment-protein complex in lipid bilayer2011

    • Author(s)
      A D.Uchiyama, H.Hoshino, K.Otomo, T.Kato, K.Onda, A.Watanabe, H.Oikawa, S.Fujiyoshi, M.Matsushita,_M.Nango, N.Watanabe, A.Sumino,_T.Dewa
    • Journal Title

      Chem.Phys.Lett.

      Volume: 511 Issue: 1-3 Pages: 135-137

    • DOI

      10.1016/j.cplett.2011.06.019

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution of bacterial photosynthetic unit in a lipid bilayer studied by single-molecule spectroscopy at 5 K2011

    • Author(s)
      Daisuke Uchiyama
    • Journal Title

      Physical chemistry chemical physics : PCCP

      Volume: 13 Issue: 24 Pages: 11615-11619

    • DOI

      10.1039/c1cp20172g

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of Akt (ser473) Phosphorylation and Rapamycin-Resistant Cell Growth by Knockdown of Mammalian Target of Rapamycin with Small Interfering RNA in Vascular Endothelial Growth Factor Receptor-1-Targeting Vector2011

    • Author(s)
      Koide, et al
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 34 Issue: 5 Pages: 602-608

    • DOI

      10.1248/bpb.34.602

    • NAID

      130000657768

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective Assembly of Photosynthetic Antenna Proteins into a Domain-Structured Lipid Bilayer for the Construction of Artificial Photosynthetic Antenna Systems : Structural Analysis of the Assembly using Surface Plasmon Resonance and Atomic Force Microscopy2011

    • Author(s)
      Sumino, Dewa, et al.
    • Journal Title

      Langmuir

      Volume: 27 Pages: 1092-1099

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution and AFM Observation of Photosynthetic Membrane Protein Assembly in Planar Lipid Bilayers2011

    • Author(s)
      Sumino, Dewa, et al.
    • Journal Title

      e-J.Surf.Sci.Nanotech.

      Volume: 9 Pages: 15-20

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dicetyl phosphate-tetraethylenepentamine-based Liposomes for Systemic siRNA Delivery2011

    • Author(s)
      Asai, et al.
    • Journal Title

      Bioconjugate Chem.

      Volume: 22 Pages: 429-435

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Liposomal Polyamine-Dialkyl Phosphate Conjugates as Effective Gene Carriers : Chemical Structure, Morphology, and Gene Transfer activity2010

    • Author(s)
      Dewa, et al.
    • Journal Title

      Bioconjugate Chem.

      Volume: 21 Pages: 844-852

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光合成でのアンテナ系タンパク質超分子複合体の構造形成とカロテノイド色素の機能評価2010

    • Author(s)
      中川勝統, ら
    • Journal Title

      高分子論文集

      Volume: 67 Pages: 574-583

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immobilization of Porphyrin Derivatives with a Defined Distance and Orientation onto a Gold Electrode Using Synthetic Light-Harvesting α-Helix Hydrophobic Polypeptides2010

    • Author(s)
      Ochiai, et al.
    • Journal Title

      Langmuir

      Volume: 26 Pages: 14419-14422

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peroxide Decoloration of CI Acid Orange 7 Catalyzed by Manganese Chlorophyll Derivatives at the Surfaces of Micelles and Lipid Bilayers2010

    • Author(s)
      Ishigure, et al.
    • Journal Title

      Langmuir

      Volume: 26 Pages: 7774-7782

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 中赤外光が誘起するタンパク質-分子の色素結合部位の構造変化2012

    • Author(s)
      大友康平, 他
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Organization and Functional Analysis of Supramolecular Assembly of Photosynthetic Antenna Proteins2012

    • Author(s)
      T.Dewa
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成膜タンパク質超分子集合体の脂質膜中への再構成と集合構造-機能相関解明2012

    • Author(s)
      角野歩, 他
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 組み換えLH1タンパク質とバクテリオクロロフィル誘導体、カロテノイドを用いたLH1複合体の再構成2012

    • Author(s)
      酒井俊亮, 他
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 酸素発生型光化学系I,II色素-タンパク質複合体の脂質二重膜への再構成と光化学活性2012

    • Author(s)
      野地智康, 他
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高等植物の膜タンパク質-色素複合体の基板上への固定化とその光電流特性2012

    • Author(s)
      近藤政晴, 他
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Polyamine-lipid/DNA Complex as a Gene Carrier : Morphological Observation and its Function2012

    • Author(s)
      Y.Urita, et al
    • Organizer
      5th International Symposium on Nanomedicine (ISNM2011)
    • Place of Presentation
      Nagoya University
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 原子間力顕微鏡によるKcsAのpH依存的開閉構造の直接観察2012

    • Author(s)
      角野歩・出羽毅久, 他
    • Organizer
      高次系分子科学第15回ミニ公開シンポジウム
    • Place of Presentation
      大阪大学(豊中キャンパス)
    • Year and Date
      2012-01-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional analysis of polyamine-lipid/nucleic acid complex (lipoplex) for nucleic acid delivery : Relationship between metamorphosis of lipoplex and its function2012

    • Author(s)
      Dewa, et al
    • Organizer
      5th International Symposium on Molecular Science of Fluctuations toward Biological Functions
    • Place of Presentation
      Todaiji Culture Center, Nara
    • Related Report
      2011 Annual Research Report
  • [Presentation] AFMによる光合成膜タンパク質の集合構造観察とその機能評価2011

    • Author(s)
      出羽毅久
    • Organizer
      第3回プローブ顕微鏡による表面分析研究会
    • Place of Presentation
      豊田中央研究所(招待講演)
    • Year and Date
      2011-12-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 生体機能解明のプラットフォームとしての脂質二分子膜2011

    • Author(s)
      出羽毅久
    • Organizer
      揺らぎと生体機能A03班班会議
    • Place of Presentation
      近江八幡市
    • Year and Date
      2011-11-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成アンテナ膜タンパク質集合体の原子間力顕微鏡による観察と機能解析2011

    • Author(s)
      出羽毅久
    • Organizer
      高次系分子化学第12回ミニ公開シンポジウム「光受容タンパク質」
    • Place of Presentation
      三谷温泉愛知県
    • Year and Date
      2011-10-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成アンテナタンパク質集合体の再構成と構造-機能評価2011

    • Author(s)
      出羽毅久, 他
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Polyamine-lipid/nucleic acid complex (lipoplex) for nucleic acid delivery : Relationship between metamorphosis of lipoplex and its function2011

    • Author(s)
      出羽毅久, 他
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Reconstitution of photosynthetic antenna protein assemblies into lipid bilayers and their evaluation of energy transfer2011

    • Author(s)
      渡部奈津子・出羽毅久, 他
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成アンテナ膜タンパク質の基板上への組織化とその光電流特性2011

    • Author(s)
      近藤政晴, 他
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成アンテナタンパク質集合体の構造-機能評価2011

    • Author(s)
      出羽毅久, 他
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成膜タンパク質集合系の構築と機能解析2011

    • Author(s)
      出羽毅久
    • Organizer
      名工大-自然科学研究機構合同講演会第2回講演会
    • Place of Presentation
      名古屋工業大学
    • Year and Date
      2011-08-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Morphological Study of Polyamine-Lipid/DNA Complexes (Lipoplexes) as Effective Gene Carriers2011

    • Author(s)
      Dewa, et al
    • Organizer
      AFM BioMed Conference, Fourth International Meeting on AFM in Life Science and Medicine
    • Place of Presentation
      Paris, France
    • Year and Date
      2011-08-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Reconstitution of photosynthetic antenna membrane protein assembly in to lipid bilayer and its direct observation using atomic force microscopy2011

    • Author(s)
      Sumino, Dewa, et al
    • Organizer
      AFM BioMed Conference, Fourth International Meeting on AFM in Life Science and Medicine
    • Place of Presentation
      Paris, France
    • Year and Date
      2011-08-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Self-assembly of photosynthetic antenna core complex onto a with defined orientation and its photocurrent activity2011

    • Author(s)
      Kondo, et al
    • Organizer
      International Conference on Tetrapyrrole Photo-receptors of Photosynthetic Organisms
    • Place of Presentation
      Berlin Germany
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular Assembly of Light-Harvesting Antenna Complexes Isolated from Spinach on Electrodes for Photoenergy Harvesting Materials2011

    • Author(s)
      Amano, et al
    • Organizer
      International Conference on Tetrapyrrole Photo-receptors of Photosynthetic Organisms
    • Place of Presentation
      Berlin Germany
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] ポリアミン脂質による遺伝子送達能とDNA複合体形態のAFM観察2011

    • Author(s)
      出羽毅久, 他
    • Organizer
      第40回医用高分子シンポジウム
    • Place of Presentation
      関西大学千里山キャンパス
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成アンテナ膜タンパク質集合体の脂質膜中への再構成と原子間力顕微鏡による集合形態の観察2011

    • Author(s)
      角野歩・出羽毅久, 他
    • Organizer
      第21回バイオ・高分子シンポジウム
    • Place of Presentation
      関西大学千里山キャンパス
    • Year and Date
      2011-07-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成アンテナ膜タンパク質集合体の脂質膜中におけるエネルギー移動評価2011

    • Author(s)
      渡部奈津子・出羽毅久, 他
    • Organizer
      第21回バイオ・高分子シンポジウム
    • Place of Presentation
      関西大学千里山キャンパス
    • Year and Date
      2011-07-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 人工光合成タンパク質/色素複合体の基板上への組織化と光電変換能評価2011

    • Author(s)
      酒井俊亮, 他
    • Organizer
      第21回バイオ・高分子シンポジウム
    • Place of Presentation
      関西大学千里山キャンパス
    • Year and Date
      2011-07-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Organization of Photosynthetic Membrane Protein Assembly and its Functional Analysis by Microscopy/Photocurrent Sensing2011

    • Author(s)
      T.Dewa
    • Organizer
      The 5^<th> Symposium Molecular Science for Supra Functional Systems
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-07-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Reconstitution and Structural/Functional Analysis of Photosynthetic Membrane Protein Assembly in Lipid Bilayers2011

    • Author(s)
      Sumino, Dewa, et al
    • Organizer
      The 5^<th> Symposium Molecular Science for Supra Functional Systems
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-07-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Immobilization of the Photosynthetic Protein Complexes with a Defined Orientation onto Solid Supports via Specific Interactions between Tagpeptide-sequences and Counterpart-ligands2011

    • Author(s)
      Sakai, et al
    • Organizer
      The 5^<th> Symposium Molecular Science for Supra Functional Systems
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-07-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成アンテナ膜タンパク質集合系の機能解明:生体分子集合系の分子科学に向けて2011

    • Author(s)
      出羽毅久
    • Organizer
      分子科学研究所所長招聘研究会「2020年の物質・生命分子科学を語る」
    • Place of Presentation
      分子科学研究所岡崎(招待講演)
    • Year and Date
      2011-06-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 脂質・核酸複合体の動的構造と細胞内核酸送達機能相関の解明2011

    • Author(s)
      出羽毅久
    • Organizer
      揺らぎが機能を決める生命分子の科学平成23年度合同班会議
    • Place of Presentation
      夕張
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Reconstitution of photosynthetic antenna membrane protein assembly into lipid bilayer and its direct observation using AFM2011

    • Author(s)
      Sumino, Dewa, et al
    • Organizer
      60^<th> SPSJ Annual Meeting
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2011-05-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Self-assembly of photosynthetic antenna membrane protein onto substrate with a defined orientation and its photocurrent activity2011

    • Author(s)
      Kondo, et al
    • Organizer
      60^<th> SPSJ Annual Meeting
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2011-05-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成のアンテナタンパク質/ポルフィリン関連色素複合体の電極基板上への組織化およびその光電流測定2011

    • Author(s)
      天野瑞貴, 他
    • Organizer
      第1回ポルフィリン-ALA研究会
    • Place of Presentation
      東京工業大学(大岡山)
    • Year and Date
      2011-05-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] ポルフィリンー脂質誘導体の合成と細胞内導入2011

    • Author(s)
      二井知紀, 他
    • Organizer
      第1回ポルフィリン-ALA研究会
    • Place of Presentation
      東京工業大学(大岡山)
    • Year and Date
      2011-05-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Reconstitution of supramolecular assembly of bacterial photosynthetic antenna/reaction center complexes into lipid bilayers : AFM observation, energy transfer, and photocurrent generation of the assembly2011

    • Author(s)
      Dewa, et al
    • Organizer
      Light-Harvesting Processes (LHP2011)
    • Place of Presentation
      Banz Monastery, Germany
    • Year and Date
      2011-04-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光合成アンテナタンパク質集合体の脂質膜中での構築とそのエネルギー移動評価2011

    • Author(s)
      渡部奈津子, ら
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成のアンテナ系-反応中心複合体のITO基板への組織化と光電流応答2011

    • Author(s)
      近藤政晴, ら
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 組み換えLH1タンパク質を用いた再構成LH1 型複合体の基板上への組織化2011

    • Author(s)
      酒井俊亮, ら
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成のアンテナタンパク質/色素複合体の電極基板上への組織化2011

    • Author(s)
      天野瑞貴, ら
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成アンテナ複合体の中赤外スペクトルの一分子観測2011

    • Author(s)
      大友康平, ら
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 異種の光合成細菌由来のアンテナおよびコア複合体を組み込んだ光合成再構成膜の高分解能AFM観察2011

    • Author(s)
      須貝祐子, ら
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成膜タンパク質分子集合体の構築と機構評価2011

    • Author(s)
      出羽毅久, ら
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 顕微鏡-電流同時計測による光合成膜タンパク質超分子集合系の機能解明2011

    • Author(s)
      出羽毅久, 他
    • Organizer
      高次系分子科学第6回合同班会議
    • Place of Presentation
      沖縄
    • Related Report
      2011 Annual Research Report
  • [Presentation] ペプチドジェミニ界面活性剤の平面二分子膜形成2011

    • Author(s)
      水野稔久, 他
    • Organizer
      高次系分子科学第6回合同班会議
    • Place of Presentation
      沖縄
    • Related Report
      2011 Annual Research Report
  • [Presentation] Supramolecular Assembly of Bacterial Light-harvesting/reaction center complexes(LH2/LH1-RC) in Planar Llipid Bilayers : AFM Observation, Energy Transfer, and Photocurrent Generation2010

    • Author(s)
      T.Dewa, et al.
    • Organizer
      The 70^<th> Okazaki Conference
    • Place of Presentation
      岡崎
    • Year and Date
      2010-12-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of Polyamine-lipid conjugates as effective gene carriers : DNA release from lipoplexes characterized by fluorescence spectroscopy2010

    • Author(s)
      Y.Kozuma, et al.
    • Organizer
      4^<th> International Symposium on Nanomedicine(ISNM2010)
    • Place of Presentation
      岡崎
    • Year and Date
      2010-12-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functional Analysis of Poluamine-Lipid/DNA Complex (Lipoplex) as Gene Carrier : Relationship Between Metamorphosis of Lipoplex and its Function2010

    • Author(s)
      T.Dewa, et al.
    • Organizer
      The 4^<th> International Symposium Molecular Science of Fluctuations toward Biological Functions
    • Place of Presentation
      滋賀
    • Year and Date
      2010-11-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 顕微鏡-電流同時計測による光合成膜タンパク質超分子集合系の機能解明2010

    • Author(s)
      出羽毅久, ら
    • Organizer
      第4回公開シンポジウム 分子高次系機能解明のための分子科学-先端計測法の開拓による素過程的理解
    • Place of Presentation
      東北大学
    • Year and Date
      2010-11-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成アンテナ・反応中心複合体の脂質二分子膜への組織化と光電流応答2010

    • Author(s)
      佐々木伸明, ら
    • Organizer
      第4回バイオ関連化学シンポジウム
    • Place of Presentation
      大阪大学豊中キャンパス
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成膜タンパク質集合体の構築と機能解析2010

    • Author(s)
      出羽毅久, ら
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道大学(札幌)(依頼講演)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成アンテナ系膜タンパク質集合体の固定化脂質二分子膜中でのエネルギー移動の評価2010

    • Author(s)
      渡部奈津子, ら
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成でのアンテナおよび反応中心複合体(LH2, LH1-RC)の脂質修飾基板上での選択的組織化2010

    • Author(s)
      矢島俊輔, ら
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Reconstitution of light harvesting 1 (LH1) complexes using zinc-substituted bacteriochlorophyll a and LH1-polypeptides isolated from purple photosynthetic bacteria together with all-trans carotenoids2010

    • Author(s)
      K.Nakagawa, et al.
    • Organizer
      Photosynthetic Light Harvesting, The Satellite Meeting to the 15^<th> International Congress of Photosynthesis
    • Place of Presentation
      Tianjin (China)
    • Year and Date
      2010-08-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] AFM Observation of Artificial Photosynthetic Antenna Protein Complexes Assembled on a Solid Membrane Support2010

    • Author(s)
      A.Sumino, et al.
    • Organizer
      Zero-Carbon Energy Kyoto 2010 The 2nd International Symposium : Kyoto University Global COE Program Energy Science in the Age of Global Warwing Toward CO2
    • Place of Presentation
      京都
    • Year and Date
      2010-08-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] LIPOSOMAL POLYAMINE-DIALKYL PHOSPHATE CONJUGATES AS EFFECTIVE GENE CARRIERS : CHEMICAL STRUCTURE, MORPHOLOGY, AND GENE TRANSFER ACTIVITY2010

    • Author(s)
      T.Dewa, et al.
    • Organizer
      International Liposome Research Days and Lipids Liposomes & Membrane Biophysics
    • Place of Presentation
      Vancouver(Canada)
    • Year and Date
      2010-08-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Organization and AFM observation of photosynthetic membrane proteins assembly in planar lipid bilayer2010

    • Author(s)
      T.Dewa
    • Organizer
      13th International Conference on Non-Contact Atomic Force Microscopy, 167 Committee satellite workshop on SPM
    • Place of Presentation
      金沢(招待講演)
    • Year and Date
      2010-08-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Reconstitution and AFM observation of Photosynthetic Membrane Protein Assembly in Artificial Planar Lipid Bilayers2010

    • Author(s)
      A.Sumino, et al.
    • Organizer
      13th International Conference on Non-Contact Atomic Force Microscopy
    • Place of Presentation
      金沢
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成膜タンパク質を含む平面脂質膜の構築と原子間力顕微鏡による直接観察2010

    • Author(s)
      角野歩, ら
    • Organizer
      第20回バイオ・高分子シンポジウム
    • Place of Presentation
      東京大学(駒場キャンパス)
    • Year and Date
      2010-07-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成アンテナ系モデルタンパク質/色素複合体の再構成と基板上への組織化2010

    • Author(s)
      酒井俊亮, ら
    • Organizer
      第20回バイオ・高分子シンポジウム
    • Place of Presentation
      東京大学(駒場キャンパス)
    • Year and Date
      2010-07-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成細菌のアンテナ系タンパク質色素複合体の再構成;カロテノイド色素の機能評価2010

    • Author(s)
      中川勝統, ら
    • Organizer
      第20回バイオ・高分子シンポジウム
    • Place of Presentation
      東京大学(駒場キャンパス)
    • Year and Date
      2010-07-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 原子間力顕微鏡による生体分子集合体の構造-機能相関解明2010

    • Author(s)
      出羽毅久
    • Organizer
      神戸大学セミナー
    • Place of Presentation
      神戸大学(六甲)
    • Year and Date
      2010-06-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光合成膜タンパク質を含む平面脂質膜の構築とAFM観察2010

    • Author(s)
      角野歩, ら
    • Organizer
      神戸大学セミナー
    • Place of Presentation
      神戸大学(六甲)
    • Year and Date
      2010-06-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 顕微鏡-電流同時計測による光合成膜タンパク質超分子集合系の機能解明2010

    • Author(s)
      出羽毅久
    • Organizer
      特定領域研究「高次系分子科学」第5回合同班会議
    • Place of Presentation
      兵庫県南あわじ市
    • Year and Date
      2010-05-24
    • Related Report
      2010 Annual Research Report
  • [Book] NON-VIRAL GENE THERAPY2011

    • Author(s)
      T.Dewa, T.Asai, N.Oku, M.Nango
    • Total Pages
      696
    • Publisher
      INTECH
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2018-03-28  

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