Direct visualization of the excited state structural dynamics of a synthetic Cu(I)-phenanthroline complex by TR-SFX method
Publicly Offered Research
| Project Area | Non-equilibrium-state molecular movies and their applications |
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| Project/Area Number |
22H04744
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Complex systems
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Maity Basudev 東京工業大学, 生命理工学院, 特任助教 (60815421)
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2023: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | TR-SFX / Mn-carbonyl complex / Lysozyme / Serial crystallography / Reaction intermediate / Ferritin cage / Copper complex / ferritin / Metal complex / Light reaction / Ferritin cage crystal / Cu(I) complex / Structural distortion |
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| Outline of Research at the Start |
Excited state structural dynamics of transition metal complexes are important not only for applications but also for rethinking the fundamental concepts. Ultrafast spectroscopy is the common method for such studies. However, real-space determination of the structural changes in the excited state remained challenging due to transient nature of the intermediate(S). In this proposal, we aim to use the TR-SFX method for observing structural dynamics of a tetrahedral Cu(I)-phen complex in ultrafast timescale.
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| Outline of Annual Research Achievements |
We immobilized a light-sensitive Mn-carbonyl complex in the porous lysozyme crystal and characterized by ATR-IR and absorbance spectroscopy. We determined the structure under darkness and compared with metal-free protein. We applied TR-SFX method to determine the changes at the metal reaction center after light irradiation. This gave interesting features such as releasing of CO ligand sequentially and the intermediate in which a water molecule was coordinated. The release of CO ligand was estimated by considering the appearance of negative density features after light irradiation which was were increased with increasing delay time. High laser intensity was found to trigger the CO release reaction. The results were validated by the QM MM calculations.
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| Research Progress Status |
令和5年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和5年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(3 results)
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[Journal Article] Real-time observation of a metal complex-driven reaction intermediate using a porous protein crystal and serial femtosecond crystallography2024
Author(s)
B. Maity, M. Shoji, F. Luo, T. Nakane, S. Abe, S. Owada, J. Kang, K. Tono, R. Tanaka, T. T. Pham, M. Kojima, Y. Hishikawa, J. Tanaka, J. Tian, M. Nagama, T. Suzuki, H. Noya, Y. Nakasuji, A. Asanuma, X. Yao, S. Iwata, Y. Shigeta, E. Nango, T. Ueno
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Journal Title
Nat. Commun
Volume: 15
Issue: 1
Pages: 5518-5518
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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