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アポトーシス誘導による疾患治療と細胞膜の揺らぎ

Publicly Offered Research

Project AreaMolecular Science of Fluctuations toward Biological Functions
Project/Area Number 23107731
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionSojo University

Principal Investigator

松本 陽子  崇城大学, 生物生命学部, 教授 (00133562)

Project Period (FY) 2011-04-01 – 2013-03-31
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywordsリウマチ / リポソーム(複合脂質膜) / 細胞膜 / 揺らぎ / アポトーシス / リポソーム(複合脂質膜)
Outline of Annual Research Achievements

生体分子の揺らぎと機能は密接であり、生体膜およびタンパク質の揺らぎは生命現象の本質を解明する上で極めて重要な課題となっている。上岡らは脂質分子とミセル分子から成るハイブリッドリポソーム(HL)が、抗がん剤を含まず、がん治療・エイズ治療が可能なことを見出している。がんやエイズ同様に難治性疾患であるリウマチやアルツハイマー病にも細胞膜の揺らぎが関与している可能性がある。
本研究では、異常増殖する患者由来のリウマチ(滑膜)細胞(HFLS-RA)に対するハイブリッドリポソーム(HL)の増殖抑制効果およびリウマチモデルマウスに対するHL-23の治療効果を検討した。①in vitroにおけるHFLS-RA細胞に対する増殖抑制試験から、HLの顕著な増殖抑制効果が明らかになった。(Bioorg. Med. Chem. Lett., 21, 207 (2011))一方で、HLは政情滑膜(HFLS)細胞に対して低毒性であることが明らかになった。②フローサイトおよびTUNEL法での解析により、HLがHFLS-RA細胞に対し、アポトーシス誘導により増殖抑制効果を示す可能性を明らかにした。③HFLS-RA細胞は、HFLSに比べて膜流動性が大きく、HLが膜流動性の高いHFLS-RA細胞に対してのみ幕融合することを明らかにした。さらに、④関節炎自然発症モデルであるSKGマウスおよびDBAマウスを用いたⅡ型コラーゲン誘発関節炎モデルに対するHLの治療実験において、未治療群では、手指関節の腫れと関節の変形がみられたが、HL治療群においては関節の炎症は見られず、リウマチに特徴的な手指の腫れや関節の変形を抑制する基礎的知見が得られた。また、組織切片の免疫染色により、HLによる炎症性サイトカインの抑制効果、軟骨保護作用およびパンヌス形成に抑制効果を明らかにしつつある。

Research Progress Status

24年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

24年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (35 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (22 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Anticancer Effects of Residual Powder from Barley-<i>Shochu</i> Distillation Remnants against the Orthotopic Xenograft Mouse Models of Hepatocellular Carcinoma <i>in Vivo</i>2012

    • Author(s)
      Masahiro Ohgidani
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 35 Issue: 6 Pages: 984-987

    • DOI

      10.1248/bpb.35.984

    • NAID

      130001872186

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intravenous injection of hybrid liposomes suppresses the liver metastases in xenograft mouse models of colorectal cancer in vivo2012

    • Author(s)
      H. Ichihara, M. Hino, M. Umebayashi, Y. Matsumoto, R. Ueoka
    • Journal Title

      Eur. J. Med. Chem

      Volume: 57 Pages: 143-148

    • DOI

      10.1016/j.ejmech.2012.08.040

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-tumor Effects of Cationic Hybrid Liposomes against Colon Carcinoma along with Apoptosis <i>in Vitro</i>2012

    • Author(s)
      M. Hino, H. Ichihara, Y. Matsumoto, R. Ueoka
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 35 Issue: 11 Pages: 2097-2101

    • DOI

      10.1248/bpb.b12-00633

    • NAID

      130001872276

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Therapeutic effects of hybrid liposomes for mouse models of adult T-cell leukemia/lymphoma in vivo2012

    • Author(s)
      Yuji Komizu
    • Journal Title

      Nano Bulletin

      Volume: 1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effect of drug-free hybrid liposomes on metastasis of human neuroblastoma2011

    • Author(s)
      Joji Yoshizawa
    • Journal Title

      Pediatric Surgery International

      Volume: 27 Issue: 4 Pages: 379-384

    • DOI

      10.1007/s00383-010-2804-1

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Membrane-targeted nanotherapy with hybrid liposomes for tumor cells leading to apoptosis2011

    • Author(s)
      Yuji Komizu
    • Journal Title

      ACS Medicinal Chemistry Letters

      Volume: 2 Issue: 4 Pages: 275-279

    • DOI

      10.1021/ml100269t

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effective Drug Delivery System for Duchenne Muscular Dystrophy Using Hybrid Liposomes Including Gentamicin along with Reduced Toxicity2011

    • Author(s)
      Mamiko Yukihara
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 34 Issue: 5 Pages: 712-716

    • DOI

      10.1248/bpb.34.712

    • NAID

      130000657785

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Experimental Therapeutic Effects of Hybrid Liposomes on the Alzheimer's Disease in Vitro2011

    • Author(s)
      座古恵子
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 131 Issue: 5 Pages: 775-782

    • DOI

      10.1248/yakushi.131.775

    • NAID

      130000678860

    • ISSN
      0031-6903, 1347-5231
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 化学工学会女性賞受賞講演 -リポソームによるがん治療2013

    • Author(s)
      松本 陽子
    • Organizer
      化学工学会第78年会
    • Place of Presentation
      大阪大学豊中キャンパス(大阪府)
    • Year and Date
      2013-03-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] Membrane-targeted nanotherapy with hybrid liposomes for cancer cells leading to apoptosis2012

    • Author(s)
      上岡 龍一
    • Organizer
      The 6th International Symposium of “Molecular Science of Fluctuation Toward Biological Functions”
    • Place of Presentation
      京都テルサ(京都府)
    • Year and Date
      2012-12-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] Inhibitory effects of hybrid liposomes on the growth of synoviocyte causing rheumatoid arthritis in vivo2012

    • Author(s)
      市原 英明
    • Organizer
      The 6th International Symposium of “Molecular Science of Fluctuation Toward Biological Functions”
    • Place of Presentation
      京都テルサ(京都府)
    • Year and Date
      2012-12-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cell-cycle regulation by hybrid liposomes for lung tumor cells2012

    • Author(s)
      古水 雄志
    • Organizer
      The 6th International Symposium of “Molecular Science of Fluctuation Toward Biological Functions”
    • Place of Presentation
      京都テルサ(京都府)
    • Year and Date
      2012-12-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] Anti-tumor effects of Cationic hybrid liposomes Against colon carcinoma along with apoptosis2012

    • Author(s)
      日野 元貴
    • Organizer
      The 6th International Symposium of “Molecular Science of Fluctuation Toward Biological Functions”
    • Place of Presentation
      京都テルサ(京都府)
    • Year and Date
      2012-12-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] New type of hybrid liposomes composed of dimyristoylphosphatidyl- choline and trehalose surfactant for the inhibition of tumor growth2012

    • Author(s)
      曹 娥
    • Organizer
      The 6th International Symposium of “Molecular Science of Fluctuation Toward Biological Functions”
    • Place of Presentation
      京都テルサ(京都府)
    • Year and Date
      2012-12-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] Anti-tumor effects of cationic Hybrid Liposomes against Colon Carcinoma along with Apoptosis in vitro2012

    • Author(s)
      日野 元貴
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(北海道)
    • Year and Date
      2012-09-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] アポトーシス誘導による疾患治療と細胞膜の揺らぎ2012

    • Author(s)
      松本 陽子
    • Organizer
      新学術領域「揺らぎと生体機能」「水和とATP」合同公開シンポ ジウム 「ゆらぎと水-生命のエネルギーと機能の分子機構を探 る」
    • Place of Presentation
      大阪ガーデンパレス(大阪府)
    • Year and Date
      2012-09-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] アポトーシス誘導による疾患治療と細胞膜の揺らぎ2012

    • Author(s)
      松本 陽子
    • Organizer
      新学術領域研究「揺らぎが機能を決める生命分子の科学」平成 24年度合同班会議
    • Place of Presentation
      作並温泉 ゆづくしSalon一の坊(宮城県)
    • Year and Date
      2012-07-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] ハイブリッドリポソームの肺がん細胞に対する細胞周期制御2012

    • Author(s)
      行原 真美子
    • Organizer
      第16回日本がん分子標的治療学会学術集会
    • Place of Presentation
      西日本総合展示場(福岡県)
    • Year and Date
      2012-06-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] Membrane-targeted therapy with hybrid liposomes leading to apoptosis2012

    • Author(s)
      Yoko Matsumoto
    • Organizer
      5th International Symposium on Nanomedicine
    • Place of Presentation
      名古屋大学ES総合館(愛知県)(招待講演)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Membrane-targeted nanotherapy with hybrid liposomes for cancer cells leading to apoptosis2012

    • Author(s)
      Ryuichi Ueoka
    • Organizer
      The International Conference on Statistical Mechanics of Liquids : From Water to Biomolecules
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県)(招待講演)
    • Year and Date
      2012-02-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitory effects of cationic hybrid liposomes against colon carcinoma leading to apoptosis2012

    • Author(s)
      Yoko Matsumoto
    • Organizer
      The International Conference on Statistical Mechanics of Liquids : From Water to Biomolecules
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県)
    • Year and Date
      2012-02-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Chemotherapy with hybrid liposomes for cancer model mice leading to apoptosis in vivo2012

    • Author(s)
      Hideaki Ichihara
    • Organizer
      The International Conference on Statistical Mechanics of Liquids : From Water to Biomolecules
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県)
    • Year and Date
      2012-02-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitory effects of cationic hybrid liposomes against colon carcinoma leading to apoptosis2012

    • Author(s)
      Motoki Hino
    • Organizer
      The 5th Open Symposium of "Molecular Science of Fluctuation Toward Biological Functions"
    • Place of Presentation
      東大寺総合文化センター(奈良県)
    • Year and Date
      2012-01-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitory effects of hybrid liposomes on the growth of synoviocyte causing rheumatoid arthritis2012

    • Author(s)
      Hideaki Ichihara
    • Organizer
      The 5th Open Symposium of "Molecular Science of Fluctuation Toward Biological Functions"
    • Place of Presentation
      東大寺総合文化センター(奈良県)
    • Year and Date
      2012-01-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Membrane-targeted therapy with hybrid liposomes in Relation to fluctuation and apoptosis2012

    • Author(s)
      Yoko Matsumoto
    • Organizer
      The 5th Open Symposium of "Molecular Science of Fluctuation Toward Biological Functions"
    • Place of Presentation
      東大寺総合文化センター(奈良県)(招待講演)
    • Year and Date
      2012-01-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cell cycle regulation by adding hybrid liposomes for tumor cells2012

    • Author(s)
      Mamiko Yukihara
    • Organizer
      The 5th Open Symposium of "Molecular Science of Fluctuation Toward Biological Functions"
    • Place of Presentation
      東大寺総合文化センター(奈良県)
    • Year and Date
      2012-01-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] アポトーシス誘導による疾患治療と細胞膜の揺らぎ2011

    • Author(s)
      松本陽子
    • Organizer
      新学術領域研究「揺らぎが機能を決める生命分子の科学」平成23年度A03班班会議
    • Place of Presentation
      休暇村近江八幡(滋賀県)
    • Year and Date
      2011-11-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitory effects of hybrid liposomes for human colon carcinoma along with apoptosis2011

    • Author(s)
      日野元貴
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] アポトーシス誘導による疾患治療と細胞膜の揺らぎ2011

    • Author(s)
      松本陽子
    • Organizer
      新学術領域研究「揺らぎが機能を決める生命分子の科学」平成23年度合同班会議
    • Place of Presentation
      ゆうばりホテルシェーパロ(北海道)
    • Year and Date
      2011-06-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸がんに対するカチオン性脂質含有ハイブリットリポソームの制がん効果2011

    • Author(s)
      日野元貴
    • Organizer
      第15回日本がん分子標的治療学会学術集会
    • Place of Presentation
      ホテル日航東京(東京都)
    • Year and Date
      2011-06-23
    • Related Report
      2011 Annual Research Report
  • [Book] Current Pharmaceutical Designs Membrane targeted chemotherapy with hybrid liposomes for tumor cells leading to apoptosis2011

    • Author(s)
      Ryuichi Ueoka
    • Total Pages
      13
    • Publisher
      Bentham Science Publishers
    • Related Report
      2011 Annual Research Report
  • [Remarks] 崇城大学生物生命学部応用生命科学科医用生体工学講座

    • URL

      http://www.life.sojo-u.ac.jp/biomed/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 文部科学省科学研究費補助金 新学術領域研究「揺らぎが機能を決める生命分子の科学」

    • URL

      http://kuchem.kyoto-u.ac.jp/hikari/yuragi/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 崇城大学生物生命学部応用生命科学科医用生体工学講座

    • URL

      http://www.life.sojo-u.ac.jp/biomed/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks] 文部科学省科学研究費補助金新学術領域揺らぎが機能を決める生体分子の科学

    • URL

      http://kuchem.kyoto-u.ac.jp/hikari/yuragi/index.html

    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2018-03-28  

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