菌類が他の生物に及ぼす「異常」を惹起する分子の探索とその活性発現機構の解明

• Project Area: Chemical Biology using bioactive natural products as specific ligands: identification of molecular targets and regulation of bioactivity
• Project/Area Number: 24102513
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Shizuoka University
• Principal Investigator: 河岸 洋和 静岡大学, グリーン科学技術研究所, 教授 (70183283)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: キノコ / 生物活性物質 / 構造決定

Research Abstract

1) 担子菌から得られたフェアリーリング惹起物質が全ての植物に成長調節活性を示すことから，植物自身もこれらの化合物を産生しているという仮説を立て検討したところ，それらの物質が植物に内生していることを証明した。さらに，それらの植物体内での生合成経路の一部も明らかにした。これらの結果は，Natureや米国化学会のChemical & Engineering Newsに紹介された。さらに，これらの物質が小麦を増産させることを圃場実験で証明した。2) スギヒラタケによる急性脳症事件の化学的解明のために，その脳症と関連が疑われるレクチンの担子菌での異種発現系を構築し，このキノコのゲノム及びトランスクリプトーム解析を行った。3) サケツバタケからは新規性の高いステロイド骨格を持つ化合物を発見した。4) アンニンコウから新規破骨細胞増殖抑制物質を発見した。5) ナラタケ培養液から新規物質Armillariol AからCを発見した。6) キシメジから新規植物成長制御物質を発見した。

Current Status of Research Progress

Reason

25年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

25年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] The source of "fairy rings": 2-azahypoxanthine and its metabolite found in a novel purine metabolic pathway in plants (2014)
  • Author(s): J-H. Choi, T. Ohnishi, Y. Yamakawa, S. Takeda, S. Sekiguchi, W. Maruyama, K. Yamashita, T. Suzuki, A. Morita, T. Ikka, R. Motohashi, Y. Kiriiwa, H. Tobina, T. Asai, S. Tokuyama, H. Hirai, N. Yasuda, K. Noguchi, T. Asakawa, S. Sugiyama, T. Kan, H. Kawagishi
  • Journal Title: Angew. Chem. Int. Ed. Volume: 53 Issue: 6 Pages: 1552-1555
  • DOI: 10.1002/anie.201308109
  • NAID: 120006727084
  • Peer Reviewed
• [Journal Article] Heterologous expression of a lectin from Pleurocybella porrigens (PPL) in Phanerochaete sordida YK-624 (2014)
  • Author(s): T. Suzuki, H. Dohra, S. Omae, Y. Takeshima, J-H. Choi, H. Hirai, H. Kawagishi
  • Journal Title: J. Microbiol. Meth. Volume: 100 Pages: 70-76
  • DOI: 10.1016/j.mimet.2014.02.016
  • NAID: 120005401892
  • Peer Reviewed
• [Journal Article] 2-Azahypoxanthine and Imidazole-4-carboxamide produced by the fairy-ring-forming fungus increase yields of wheat (2014)
  • Author(s): H. Tobina, J-H. Choi, T. Asai, Y. Kiriiwa, T. Asakawa, T. Kan, A. Morita, H. Kawagishi
  • Journal Title: Field Crop Res Volume: 162 Pages: 6-11
  • DOI: 10.1016/j.fcr.2014.03.008
  • Peer Reviewed
• [Journal Article] Isolation of Bioactive Steroids from the <i>Stropharia rugosoannulata</i> Mushroom and Absolute Configuration of Strophasterol B (2013)
  • Author(s): Wu J., Kobori H., Kawaide M., Suzuki T., Choi J. -H., Yasuda N., Noguchi K., Matsumoto T., Hirai H., Kawagishi H.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 8 Pages: 1779-1781
  • DOI: 10.1271/bbb.130216
  • NAID: 10031195834
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Dimerization of Bisphenol A by Hyper Lignin-Degrading Fungus Phanerochaete sordida YK-624 Under Ligninolytic ConditionDimerization of Bisphenol A by Hyper Lignin-Degrading Fungus Phanerochaete sordida YK-624 Under Ligninolytic Condition (2013)
  • Author(s): J. Wang, H. Hirai, H. Kawagishi, Dimerization of bisphenol A by hyper lignin-degrading fungus Phanerochaete sordida YK-624 under ligninolytic condition,
  • Journal Title: Curr. Microbiol. Volume: 66 Issue: 6 Pages: 544-547
  • DOI: 10.1007/s00284-013-0310-0
  • Peer Reviewed
• [Journal Article] Improvement of manganese peroxidase production by the hyper lignin-degrading fungus Phanerochaete sordida YK-624 by recombinant expression of the 5-aminolevulinic acid synthase gene (2013)
  • Author(s): H. Hirai, K. Misumi, T. Suzuki, T., H. Kawagishi
  • Journal Title: Curr. Microbiol. Volume: 67 Issue: 6 Pages: 708-711
  • DOI: 10.1007/s00284-013-0428-0
  • NAID: 120005463455
  • Peer Reviewed
• [Journal Article] A new omics data resource of Pleurocybella porrigens for gene discovery (2013)
  • Author(s): T. Suzuki, K. Igarashi, H. Dohra, T. Someya, T. Takano, K. Harada, S. Omae, S., H. Hirai, K. Yano, H. Kawagishi
  • Journal Title: PloS ONE Volume: 8 Issue: 7 Pages: e69681-e69681
  • DOI: 10.1371/journal.pone.0069681
  • NAID: 120005304932
  • Peer Reviewed
• [Journal Article] An unusual sterol from the mushroom Stropharia rugosoannulata (2013)
  • Author(s): Wu J., Suzuki T., Choi J. -H., Yasuda N., Noguchi K., Hirai H., Kawagishi H.
  • Journal Title: Tetrahedron Letters Volume: 54 Issue: 36 Pages: 4900-4902
  • DOI: 10.1016/j.tetlet.2013.06.142
  • Peer Reviewed
• [Journal Article] Hydroxylation of bisphenol A by hyper lignin-degrading fungus Phanerochaete sordida YK-624 undernon-ligninolytic condition, (2013)
  • Author(s): J. Wang, R. Yamamoto, Y. Yamamoto, T. Tokumoto, J. Dong, J., P. Thomas,H. Hirai, H Kawagishi
  • Journal Title: Chemosphere Volume: 93 Issue: 7 Pages: 1419-1423
  • DOI: 10.1016/j.chemosphere.2013.07.026
  • NAID: 120005317558
  • Peer Reviewed
• [Journal Article] A novel sphingosine with osteoclast-forming suppressing activity, from the edible mushroom Grifola gargal (2013)
  • Author(s): J-H. Choi, M. Yoshida, T. Suzuki, E. Harada, M. Kawade, K. Yazawa, S. Nishimoto, H. Hirai, H. Kawagishi
  • Journal Title: Tetrahedron Volume: 69 Issue: 40 Pages: 8609-8611
  • DOI: 10.1016/j.tet.2013.07.068
  • NAID: 120005342112
  • Peer Reviewed
• [Journal Article] Armillariols A to C from the culture broth of Armillaria sp. (2013)
  • Author(s): H. Kobori, A. Sekiya, N. Yasuda, K. Noguchi, T. Suzuki, J-H. Choi, H. Hirai, H. Kawagishi
  • Journal Title: Tetrahedron Lett. Volume: 54 Issue: 40 Pages: 5481-5483
  • DOI: 10.1016/j.tetlet.2013.07.131
  • NAID: 120005342113
  • Peer Reviewed
• [Journal Article] Biotransformation of acetamiprid by the white-rot fungus Phanerochaete sordida YK-624 (2012)
  • Author(s): J. Wang, H. Kawagishi
  • Journal Title: Appl. Microbiol. Biotechnol Volume: 93 Pages: 831-835
  • NAID: 120004226433
  • Peer Reviewed
• [Journal Article] Degradation of target oligosaccharides by anthraquinone-lectin hybrids with light switching (2012)
  • Author(s): Y. Imai, S. Hirono, H. Matsuba, T. Suzuki, Y. Kobayashi, H. Kawagishi, D. Takahashi, K. Toshima
  • Journal Title: Chemistry-An Asian Journal Volume: 7 Issue: 1 Pages: 97-104
  • DOI: 10.1002/asia.201100586
  • Peer Reviewed
• [Journal Article] Agrocybynes A to E from the culture broth of Agrocybe praecox (2012)
  • Author(s): K. Fushimi, H. Kawagishi
  • Journal Title: Tetrahedron Volume: 68 Issue: 4 Pages: 1262-1265
  • DOI: 10.1016/j.tet.2011.11.049
  • NAID: 120004186785
  • Peer Reviewed
• [Journal Article] Mannose-specific lectin from the mushroom Hygrophorus russula (2012)
  • Author(s): Suzuki, T.
  • Journal Title: Glycobiology Volume: 22 Issue: 5 Pages: 616-629
  • DOI: 10.1093/glycob/cwr187
  • Peer Reviewed
• [Journal Article] Novel Cerebroside, Termitomycesphin I, from the Mushroom, <i>Termitomyces titanicus</i> (2012)
  • Author(s): J-H. Choi, H. Kawagishi
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 7 Pages: 1407-1409
  • DOI: 10.1271/bbb.120130
  • NAID: 130001862732
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Makomotindoline from Makomotake, Zizania latifolia infected with Ustilago esculenta (2012)
  • Author(s): T. Suzuki, H. Kawagishi
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 22 Issue: 13 Pages: 4246-4248
  • DOI: 10.1016/j.bmcl.2012.05.021
  • NAID: 120004186786
  • Peer Reviewed
• [Journal Article] A novel core fucose-specific lectin from the mushroom Pholiota squarrosa (2012)
  • Author(s): Y. Kobayashi,, H. Kawagishi
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 33973-33978
  • NAID: 120005308337
  • Peer Reviewed
• [Journal Article] Strophasterols A to D with an Unprecedented Steroid Skeleton : From the Mushroom Stropharia rugosoannulata (2012)
  • Author(s): Jing Wu, et al.
  • Journal Title: Angew. Chem. Int. Ed. Volume: 51 Issue: 43 Pages: 10820-10822
  • DOI: 10.1002/anie.201205351
  • Peer Reviewed
• [Presentation] Gargalols A, B and C, Osteoclast-Forming Suppressing Compounds from the Edible Mushroom Grifola gargal (2013)
  • Author(s): Hirokazu Kawagishi
  • Organizer: The 7th International Medicinal Mushroom Conference
  • Place of Presentation: Beijing, China
  • Invited
• [Presentation] Disclosure of the “Fairy” of Fairy-ring Forming Fungus Lepista sordida (2013)
  • Author(s): Hirokazu Kawagishi
  • Organizer: 13th International Conference on the Chemistry of Antibiotics and other bioactive compounds (ICCA)
  • Place of Presentation: 山梨県富士ビューホテル（山梨県）
  • Invited
• [Presentation] Disclosure of the “fairy” of fairy-ring forming fungus Lepista sordida – Discovery of a novel purine metabolic pathway in plants – (2013)
  • Author(s): Hirokazu Kawagishi
  • Organizer: The 2nd International Symposium on Chemical Biology of Natural Products: Target ID and Regulation of Bioactivit
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Invited
• [Presentation] Disclosure of the “fairies” of fairy-ring forming fungus Lepista sordida and possibility of their application to agriculture (2013)
  • Author(s): Hirokazu Kawagishi
  • Organizer: The 50th Memorial Symposium on Phytochemistry
  • Place of Presentation: 東京大学（東京都）
  • Invited
• [Presentation] Search for fruiting body inducing compounds in higher fungi – Strophasterols A to D with an unprecedented steroid skeleton: candidates for common fruiting body-inducers in mushrooms – (2013)
  • Author(s): Hirokazu Kawagishi
  • Organizer: International Symposium on Natural Products Chemistry and Chemical Biology 2013
  • Place of Presentation: ホテル ルブラ王山（愛知県）
  • Invited
• [Presentation] キノコの生体調節機能とそれを司る化合物 (2013)
  • Author(s): 河岸洋和
  • Organizer: 第５回食品薬学シンポジウム
  • Place of Presentation: 京都大学薬学部記念講堂（京都府）
  • Invited
• [Presentation] The angel’s wing mystery-Attempt to disclose the molecular mechanism of acute encephalopathy caused by eating angel’s wing oyster mushroom (Sugihiratake) (2012)
  • Author(s): Hirokazu Kawagishi
  • Organizer: International Symposium on Natural Products Chemistry and Chemical Biology 2012
  • Place of Presentation: Hangzhou (China)
  • Year and Date: 2012-11-22
  • Invited
• [Remarks]
  • URL: http://www.agr.shizuoka.ac.jp/c/biochem/index.html