アロ認証関連遺伝子の進化遺伝学的解析

• Project Area: Elucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants
• Project/Area Number: 24112702
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University (2013); Yamagata University (2012)
• Principal Investigator: 田宮 元 東北大学, 東北メディカル・メガバンク機構, 教授 (10317745)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞・組織 / CD9 / Izumo / 配偶子認識 / 生殖 / 進化遺伝学

Research Abstract

本研究の研究目標は、CD9 のように当該特別推進研究領域で取得されているアロ認証に関連する遺伝子の塩基配列をTajima のD や非同義置換率と同義置換率の比ω、Fay とWu のH のような進化遺伝学的統計量を用いて評価して、その特有の進化的パターンを探り、それらの知見から、アロ認証にどのような中核原理が可能であるのか予言するというものである。平成25年度中には、CD9とIZUMO1を代表例として、1000人ゲノムプロジェクトからDNAバリエーションデータを取得し、TajimaのDとω（dN/dS）を計測したところ、CD9はD<<0かつω<1と強い純化淘汰の対象にあるが、IZUMO1はむしろω>1と迅速進化の対象となっていることが示された。興味深いことに、更なる調査で、IZUMO1がヒト集団中で高度のCNV多型領域のひとつであることが判明し、その他にもかなりの数の生殖関連遺伝子がCNV領域中にあって、実際のヒト集団でコピー数多型を示すことが分かった。このようなゲノム浮動が、今後、アロ認証関連遺伝子の進化メカニズムに興味深い示唆を与えるものであると期待される。

Current Status of Research Progress

Reason

25年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

25年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Divergence of East Asians and Europeans estimated using maleand female-specific genetic markers (2014)
  • Author(s): Tateno Y, Komiyama T, Katoh T, Munkhbat B, Oka A, Haida Y, Kobayashi H, Tamiya G, Inoko H
  • Journal Title: Genome Biol Evol Volume: 6(3) Issue: 3 Pages: 466-73
  • DOI: 10.1093/gbe/evu027
  • Peer Reviewed
• [Journal Article] A pollen coat-inducible autoinhibited Ca^<2+>-ATPase expressed in stigmatic papilla cells is required for compatible pollination in the Brassicaceae (2014)
  • Author(s): Iwano, M., Igarashi, M., Tarutani, Y., Kaothien-Nakayama, P., Nakayama, H., Moriyama, H., Yakabe, R., Entani, T., Shimosato-Asano, H., Ueki, M., Tamiya, G., and Takayama, S
  • Journal Title: Plant Cell Volume: 26 (2) Issue: 2 Pages: 636-649
  • DOI: 10.1105/tpc.113.121350
  • Peer Reviewed
• [Journal Article] Pleiotropic effect of common variants at ABO glycosyltranferase locus in 9q32 on plasma levels of pancreatic lipase and angiotensin converting enzyme (2014)
  • Author(s): Yamagata University Genomic Cohort Consortium (YUGCC)
  • Journal Title: PLOS ONE Volume: 9 Issue: 2 Pages: e55903-e55903
  • DOI: 10.1371/journal.pone.0055903
  • Peer Reviewed
• [Journal Article] Variants in melanogenesis-related genes associate with skin cancer risk among Japanese populations. (2014)
  • Author(s): Yoshizawa J, Abe Y, Oiso N, Fukai K, Hozumi Y, Nakamura T, Narita T, Motokawa T, Wakamatsu K, Ito S, Kawada A, Tamiya G, Suzuki T
  • Journal Title: J Dermatol Volume: 41 Issue: 4 Pages: 296-302
  • DOI: 10.1111/1346-8138.12432
  • Peer Reviewed
• [Journal Article] Gamete attachment requires GEX2 for successful fertilization in Arabidopsis. (2014)
  • Author(s): 森稔幸、井川智子、田宮元、宮城島進也、Frederic Berger
  • Journal Title: Current Biology Volume: 24 Issue: 2 Pages: 170-175
  • DOI: 10.1016/j.cub.2013.11.030
  • Peer Reviewed
• [Journal Article] Case of a Mongolian child with extensive Mongolian spots in Mucopolysaccharidosis type VI: Identification of a novel mutation in the arylsulfatase B gene. (2013)
  • Author(s): Okamura K, Munkhbat B, Bachimeg B, Tamiya G, Hozumi Y, Suzuki T.
  • Journal Title: J Dermatol Volume: 40 Issue: 9 Pages: 758-759
  • DOI: 10.1111/1346-8138.12237
  • Peer Reviewed
• [Journal Article] Comparison of caregiver strain in Parkinson's disease between Yamagata, Japan, and Maryland, The United States. (2013)
  • Author(s): Tanji H, Koyama S, Wada M, Kawanami T, Kurita K, Tamiya G, Saito N, Suzuki K, Kato T, Anderson KE, Gruber-Baldini AL, Fishman PS, Reich SG, Weiner WJ, Shulman LM.
  • Journal Title: Parkinsonism Relat Disord Volume: 19 Issue: 6 Pages: 628-633
  • DOI: 10.1016/j.parkreldis.2013.02.014
  • Peer Reviewed
• [Journal Article] The confounding effect of cryptic relatedness for environmental risks of systolic blood pressure on cohort studies. (2013)
  • Author(s): Shibata K, Hozawa A, Tamiya G, Ueki M, Nakamura T, et al.
  • Journal Title: Mol Genet Genomic Med Volume: 1 Issue: 1 Pages: 45-53
  • DOI: 10.1002/mgg3.4
  • Peer Reviewed
• [Journal Article] A founder haplotype of APOE-Sendai mutation associated with lipoprotein glomerulopathy. (2013)
  • Author(s): Toyota K, Hashimoto T, Ogino D, Matsunaga A, Ito M, Masakane I, Degawa N, Sato H, Shirai S, Umetsu K, Tamiya G, Saito T, Hayasaka K.
  • Journal Title: J Hum Genet Volume: 58 Pages: 254-258
  • NAID: 10031177219
  • Peer Reviewed
• [Journal Article] Association of breast-fed neonatal hyperbilirubinemia with UGT1A1 polymorphisms: 211G>A (G71R) mutation becomes a risk factor under inadequate feeding (2013)
  • Author(s): Sato H, Uchida T, Toyota K, Kanno M, Hashimoto T, Watanabe M, Nakamura T, Tamiya G, Aoki K, Hayasaka K.
  • Journal Title: J Hum Genet Volume: 58(1) Issue: 1 Pages: 7-10
  • DOI: 10.1038/jhg.2012.116
  • NAID: 10031145893
  • Peer Reviewed
• [Journal Article] Constructinga contemporary gene-environmental cohort: study design of the Yamagata Molecular Epidemiological Cohort Study (2013)
  • Author(s): Yamagata University Genomic CohortConsortium. Tamiya G、UekiM, 他57名
  • Journal Title: J Hum Genet Volume: 58(1) Issue: 1 Pages: 54-56
  • DOI: 10.1038/jhg.2012.128
  • Peer Reviewed
• [Journal Article] Association of melanogenesis genes with skin color variation among Japanese females. (2013)
  • Author(s): Abe Y
  • Journal Title: J Dermatol Sci Volume: 69 Pages: 167-172
  • Peer Reviewed
• [Journal Article] Generation of a Monoclonal Antibody Specifically Reacting with Neuron-specific TATA-Box Binding Protein-Associated Factor 1 (N-TAF1) (2013)
  • Author(s): Makino S, Masuda C, Ando S, Tamiya G, Tooyama I.
  • Journal Title: Antibodies Volume: 2 Issue: 1 Pages: 1-8
  • DOI: 10.3390/antib2010001
  • Peer Reviewed
• [Journal Article] Ultrahigh-dimensional variable selection method for whole-genome gene-gene interaction analysis (2013)
  • Author(s): Ueki M, Tamiya G.
  • Journal Title: BMC Bioinformatics Volume: 13 Issue: 1 Pages: 72-72
  • DOI: 10.1186/1471-2105-13-72
  • Peer Reviewed
• [Journal Article] HLA-A*0206 with TLR3 Polymorphisms Exerts More than Additive Effects in Stevens-Johnson Syndrome with Severe Ocular Surface Complications (2012)
  • Author(s): Ueta M, Tokunaga K, Sotozono C, Sawai H, Tamiya G, Inatomi T, Kinoshita S
  • Journal Title: PLoS One, Peer-Reviewed papers Volume: 7(8) Issue: 8 Pages: e43650-e43650
  • DOI: 10.1371/journal.pone.0043650
  • Peer Reviewed
• [Journal Article] Epistatic interaction between Toll-like receptor 3 (TLR3) and prostaglandin E receptor 3 (PTGER3) genes (2012)
  • Author(s): Ueta M, Tamiya G, Tokunaga K, Sotozono C, Ueki M, Sawai H, Inatomi T, Matsuoka T, Akira S, Narumiya S, Tashiro K, Kinoshita S
  • Journal Title: J Allergy Clin Immunol Peer-Reviewed papers Volume: 129(5) Issue: 5 Pages: 1413-1416
  • DOI: 10.1016/j.jaci.2012.01.069
  • Peer Reviewed
• [Journal Article] UBR5 Gene Mutation Is Associated with Familial Adult Myoclonic Epilepsy in a Japanese Family (2012)
  • Author(s): Kato T, Tamiya G, Koyama S, Nakamura T, Makino S, Arawaka S, Kawanami T, Tooyama I.
  • Journal Title: ISRN Neurol Volume: 2012 Pages: 508308-508308
  • DOI: 10.5402/2012/508308
  • Peer Reviewed
• [Journal Article] Sustained expression of a neuron-specific isoform of the Taf1 gene in development stages and aging in mice (2012)
  • Author(s): Jambaldorj J, Makino S, Munkhbat B, Tamiya G.
  • Journal Title: Biochem Biophys Res Commun Volume: 425(2) Issue: 2 Pages: 273-277
  • DOI: 10.1016/j.bbrc.2012.07.081
  • Peer Reviewed
• [Presentation] アロ認証関連遺伝子の進化遺伝学的解析 (2014)
  • Author(s): 田宮 元
  • Organizer: 第8回領域会議
  • Place of Presentation: 名古屋市
• [Presentation] アロ認証関連遺伝子の進化遺伝学的解析 (2013)
  • Author(s): 田宮 元
  • Organizer: 第7回領域会議
  • Place of Presentation: 松江市
• [Presentation] Genome-wide gene-gene interaction analysis based on ultrahigh-dimensional variable selection. (2012)
  • Author(s): Ueki M
  • Organizer: 5th International Conference of the ERCIM WG on COMPUTING STATISTICS (ERCIM 2012)
  • Place of Presentation: オビエドカンファレンスセンター（オビエド・スペイン）
  • Year and Date: 2012-12-01
  • Invited
• [Presentation] Molecular evolution of IZUMO1 and CD9; genes involved in reproductive fitness．International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants. (2012)
  • Author(s): Tamiya G
  • Organizer: Joint Meeting of the 2nd Allo-Authentication Meeting and the 5th Egg-Coat Meeting (MCBEEC)
  • Place of Presentation: ホテル名古屋ガーデンパレス（名古屋）
  • Year and Date: 2012-11-12
• [Presentation] adaptiveデザインのゲノム疫学研究への適用．日本人類遺伝学会第57回大会, 東京 (2012)
  • Author(s): 中村 智洋
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 京王プラザホテル（東京）
  • Year and Date: 2012-10-24
• [Presentation] 高次元変数選択法によるゲノムワイド遺伝子-遺伝子および遺伝子-環境相互作用解析法 (2012)
  • Author(s): 植木 優夫
  • Organizer: 日本人類遺伝学会第57回大会
  • Place of Presentation: 京王プラザホテル（東京）
  • Year and Date: 2012-10-24
• [Presentation] 役に立つ集団遺伝学 (2012)
  • Author(s): 田宮 元
  • Organizer: 第5回生殖研究若手の会
  • Place of Presentation: 東京大学三崎臨海実験所（神奈川）
  • Year and Date: 2012-07-26