植物の配偶子融合および受精卵分裂過程における金属元素制御機構の解明

• Project Area: Elucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants
• Project/Area Number: 24112704
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Utsunomiya University
• Principal Investigator: 高橋 美智子 宇都宮大学, 農学部, 准教授 (90345182)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: イネ / 亜鉛 / 卵細胞

Outline of Annual Research Achievements

鉄、亜鉛、銅、マンガンなど必須金属元素の欠乏により花粉形成の異常、不稔、収量低下が起こる。これら必須元素の重要性にも関わらず、植物の受精および胚発生過程における金属元素の役割の詳細は未だ解明されていない。本研究ではイネの受精(植物における配偶子融合過程)および受精卵の細胞分裂過程（初期胚発生）における亜鉛の役割を明らかにすることを目的とする。イネ単離配偶子を材料とし、in vitro受精系を用いて、亜鉛の配偶子融合への影響、イネ卵への亜鉛の供給形態、亜鉛結合タンパク、亜鉛関連の遺伝子が受精および受精卵の細胞分裂過程にどのように関連するか明らかにする。２６年度はイネ未受精卵への亜鉛欠乏の影響の解析を中心に研究を行った。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Enhanced levels of nicotianamine promote iron accumulation and tolerance to calcareous soil in soybean. (2014)
  • Author(s): Tomoko Nozoye, Suyoen Kim, Yusuke Kakei, Michiko Takahashi, Hiromi Nakanishi & Naoko K. Nishizawa
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 78 Pages: 1677-1684
  • Peer Reviewed
• [Journal Article] Rice nicotianamine synthase localizes to particular vesicles for proper function (2014)
  • Author(s): Nozoye T, Tsunoda K, Nagasaka S, Bashir K, Takahashi M, Kobayashi T, Nakanishi H, Nishizawa NK
  • Journal Title: Plant Signaling and Behavior Volume: 9 Issue: 5 Pages: e28660-e28660
  • DOI: 10.4161/psb.28660
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXφ or LL motif causes the disruption of vesicle formation or movement in rice. (2014)
  • Author(s): Nozoye T, Nagasaka S, Bashir K, Takahashi M, Kobayashi T, Nakanishi H, Nishizawa NK.
  • Journal Title: The Plant Journal Volume: 77 Pages: 246-260
  • Peer Reviewed
• [Journal Article] Dynamic changes in the distribution of minerals in relation to phytic acid accumulation during rice seed development (2012)
  • Author(s): Iwai, T., Takahashi, M., Oda, K., Terada, Y., Yoshida, K. T.
  • Journal Title: Plant Physiology Volume: 160 Issue: 4 Pages: 2007-2014
  • DOI: 10.1104/pp.112.206573
  • Peer Reviewed
• [Journal Article] Accumulation of starch in Zn-deficient rice (2012)
  • Author(s): Suzuki M, Bashir K, Inoue H, Takahashi M, Nakanishi H, Nishizawa NK.
  • Journal Title: Rice Volume: 5 Issue: 1 Pages: 9-9
  • DOI: 10.1186/1939-8433-5-9
  • Peer Reviewed
• [Journal Article] Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition. (2012)
  • Author(s): Masuda H, Ishimaru Y, May Sann Aung, Kobayashi T, Kakei Y, Takahashi M, Higuchi K, Nakanishi H, Nishizawa NK
  • Journal Title: Scientific Reports Volume: 2 Issue: 1 Pages: 543-543
  • DOI: 10.1038/srep00543
  • Peer Reviewed
• [Presentation] Analysis of functions of AtbZIP19 in rice plant (2014)
  • Author(s): Aiko Watanabe, Hirokuni Ikezawa, Tetsuya Shimoyama, Vanessa Stein, Mark GM Aarts, Michiko Takahashi
  • Organizer: the 17th International Symposium on Iron Nutrition and Interactions in Plants (ISINIP)
  • Place of Presentation: ドイツ（Gatersleben/Quedlinburg）
  • Year and Date: 2014-07-07
• [Presentation] 生殖生長と種子成熟の植物栄養学からめざす作物の収量増加と機能性向上 (2012)
  • Author(s): 高橋美智子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取大学
  • Year and Date: 2012-09-06
• [Presentation] シロイヌナズナ亜鉛欠乏応答性転写因子AtbZIP19のイネにおける機能解析 (2012)
  • Author(s): 渡辺藍子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取大学
  • Year and Date: 2012-09-06