マラリア原虫ＧＣＳ１を基盤とした受精メカニズムの解明

• Project Area: Elucidation of Common Mechanisms for Allogeneic Authentication in Animals and Plants
• Project/Area Number: 24112705
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Juntendo University (2013); Gunma University (2012)
• Principal Investigator: 平井 誠 順天堂大学, 医学部, 准教授 (50326849)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: マラリア原虫 / ネズミマラリア原虫 / 受精 / PbGCS1

Outline of Annual Research Achievements

ネズミマラリア原虫Plasmodium bergheiのオス側の受精因子であるGCS1に関し、各種欠損変異体を作成し、その受精能を調べた。その結果、GCS1の細胞質側領域は受精には一切必要なく、細胞膜貫通ドメインを含む細胞外ドメインが必要であることを明らかにした。このことは、GCS1が細胞膜上に存在し、メス側に存在することが推定されるGCS1受容体との結合を経て受精が行われること考えられる。GCS1以外の新規な受精因子を探索するため、雌雄の生殖母体で発現している遺伝子を候補として順次ノックアウトをした結果、メス側の受精に関わる因子を見つけることに成功した（投稿準備中）。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Direct evidence for the atovaquone action on the Plasmodium cytochrome bc1 complex. (2015)
  • Author(s): Siregar JE, Kurisu G, Kobayashi T, Matsuzaki M, Sakamoto K, Mi-Ichi F, Watanabe Y, Hirai M, Matsuoka H, Syafruddin D, Marzuki S, Kita K.
  • Journal Title: Parasitol Int Volume: 64 Issue: 3 Pages: 295-300
  • DOI: 10.1016/j.parint.2014.09.011
  • Peer Reviewed / Open Access
• [Journal Article] Lactate retards the development of erythrocytic stages of the human malaria parasite Plasmodium falciparum. (2015)
  • Author(s): Hikosaka K, Hirai M, Komatsuya K, Ono Y, Kita K.
  • Journal Title: Parasitol Int Volume: 64 Issue: 3 Pages: 301-303
  • DOI: 10.1016/j.parint.2014.08.003
  • Peer Reviewed / Open Access
• [Journal Article] Evaluating experimental cerebral malaria using oxidative stress indicator OKD48 mice. (2014)
  • Author(s): Imai T, Iwawaki T, Akai R, Suzue K, Hirai M, Taniguchi T, Okada H, Hisaeda H.
  • Journal Title: Int J Parasitol. Volume: 44 Issue: 10 Pages: 681-685
  • DOI: 10.1016/j.ijpara.2014.06.002
  • Peer Reviewed / Open Access
• [Journal Article] Generation of rodent malaria parasites with a high mutation rate by destructing proofreading activity of DNA polymerase δ. (2014)
  • Author(s): Honma H, Hirai M, Nakamura S, Hakimi H, Kawazu S, Palacpac NM, Hisaeda H, Matsuoka H, Kawai S, Endo H, Yasunaga T, Ohashi J, Mita T, Horii T, Furusawa M, Tanabe K.
  • Journal Title: DNA Res Volume: 21 Issue: 4 Pages: 439-46
  • DOI: 10.1093/dnares/dsu009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CD8(+) T cell activation by murine erythroblasts infected with malaria parasites. (2013)
  • Author(s): Imai T, Ishida H, Suzue K, Hirai M, Taniguchi T, Okada H, Suzuki T, Shimokawa C, Hisaeda H.
  • Journal Title: Sci Rep. Volume: 3 Issue: 1 Pages: 1572-1572
  • DOI: 10.1038/srep01572
  • NAID: 120006985954
  • Peer Reviewed
• [Journal Article] Resistance to Malaria by Enhanced Phagocytosis of Erythrocytes in LMP7-deficient Mice. (2013)
  • Author(s): Duan X, Imai T, Chou B, Tu L, Himeno K, Suzue K, Hirai M, Taniguchi T, Okada H, Shimokawa C, Hisaeda H.
  • Journal Title: PLoS One. Volume: 8 Issue: 3 Pages: 1-9
  • DOI: 10.1371/journal.pone.0059633
  • Peer Reviewed
• [Journal Article] Toward understanding the role of mitochondrial complex II in the intraerythrocytic stages of Plasmodium falciparum: gene targeting of the Fp subunit. (2012)
  • Author(s): Tanaka TQ, Hirai M, Watanabe Y, Kita K.
  • Journal Title: Parasitol Int Volume: 61 Issue: 4 Pages: 726-8
  • DOI: 10.1016/j.parint.2012.06.002
  • Peer Reviewed
• [Journal Article] Critical roles of the mitochondrial complex II in oocyst formation of rodent malaria parasite Plasmodium berghei. (2012)
  • Author(s): Hino A, Hirai M, Tanaka TQ, Watanabe Y, Matsuoka H, Kita K.
  • Journal Title: J Biochem Volume: 152 Issue: 3 Pages: 259-68
  • DOI: 10.1093/jb/mvs058
  • NAID: 10031166137
  • Peer Reviewed
• [Journal Article] One Injection of DsRed Followed by Bites from Transgenic Mosquitoes Producing DsRed in the Saliva Elicits a High Titer of Antibody in Mice (2012)
  • Author(s): Matsuoka H, Sano G, Hattori R, Tomita H, Yamamoto DS, Hirai M.
  • Journal Title: Tropical Medicine and Health Volume: 40 Issue: 2 Pages: 47-53
  • DOI: 10.2149/tmh.2011-10
  • NAID: 10031123157
  • ISSN: 1348-8945, 1349-4147
  • Peer Reviewed
• [Presentation] 比較トランスクリプトームによるマラリア原虫受精因子の探索 (2012)
  • Author(s): 平井誠、安彦真文、岡本龍司、本間一、鈴江一友、今井孝、堀井俊宏、田邉和裄、久枝一
  • Organizer: 第１０回分子寄生虫・マラリアフォーラム、第７２回日本寄生虫学会東日本支部会 合同大会
  • Place of Presentation: 群馬大学医学部
• [Presentation] マラリア原虫をやっつける～その驚異、生態、新たな対策 (2012)
  • Author(s): 平井 誠
  • Organizer: 第2回 WIAS Top Runners’ Lecture Collection of Science
  • Place of Presentation: 早稲田大学高等研究所
  • Invited
• [Book] [動植物の受精学] 共通機構と多様性 (2014)
  • Author(s): 森稔幸、平井 誠
  • Total Pages: 11
  • Publisher: 化学同人
• [Book] Sexual Reproduction in Animals and Plants (2014)
  • Author(s): HIRAI M
  • Publisher: Elsevier