元素ブロックによる高分子界面の構造・物性制御

• Project Area: Creation of Element-Block Polymer Materials
• Project/Area Number: 25102535
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kyushu University
• Principal Investigator: 田中 敬二 九州大学, 工学(系)研究科(研究院), 教授 (20325509)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: POSS / 末端基 / 界面濃縮 / 分子鎖熱運動性

Outline of Annual Research Achievements

リビングアニオン重合に基づき、ポリメタクリル酸メチル（PMMA）の両末端にかご型シルセスキオキサン（POSS）ブロックを導入した高分子PPMPを合成した[Mn(GPC) = 28.7k, Mw/Mn = 1.31]。比較のため、PMMA[Mn = 25k, Mw/Mn = 1.09]も試料として用いた。
細胞の接着・増殖特性は、マウス結合組織由来の線維芽細胞L929を用いて評価した。細胞は、無血清もしくは10%ウシ血清(FBS)含有RPMI 1640培地において標準的な培養条件下で培養した。位相差顕微鏡を用いて観察したPMMA膜およびPPMP膜上の細胞接着数および形態を評価した。PPMP膜上に接着した細胞の数は、PMMA膜上のそれと比較して少なく、また、接着面積ならびに伸展率も低かった。これは、疎水性の高いPOSS部が濃縮した膜表面と細胞が強く相互作用するため、分裂および増殖プロセスへの移行が抑制されたことを示している。血清存在下においても、PPMP膜上における細胞は、PMMA膜上におけるそれと比較し、細胞活性が抑制されることがわかった。血小板粘着特性は、正常ヒト全血より調製した多血小板血漿を用いて評価した。各膜は、前処理としてPBS中に所定時間浸漬した後、試験に供した。PPMP膜上に粘着した血小板の数は、PMMA膜上のそれより多く、また、より多くの偽足形成と扁平化が認められた。PMMA膜における血小板数は前処理時間に依らずほぼ一定であるのに対し、PPMP膜におけるそれは、前処理時間の経過に伴い減少した。
これまで得られた成果を総括することで、膜表面における分子鎖凝集状態とその環境応答性が、血小板粘着特性に強く影響を与えることが明らかとなり、POSSを用いた高分子界面設計の設計指針が得られた。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Time-resolved Fluorescence Analysis for Dye-labeled Polystyrene in Thin Films (2015)
  • Author(s): D. Kawaguchi, Y. Tateishi, K. Tanaka
  • Journal Title: Journal of Non-Crystalline Solids Volume: 407 Pages: 284-287
  • DOI: 10.1016/j.jnoncrysol.2014.09.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dynamic-driven Surface Segregation of a Hydrophilic Component in Diblock Copolymer Films (2015)
  • Author(s): Cui Zhang, Yukari Oda, Daisuke Kawaguchi, Shokyoku Kanaoka, Sadahito Aoshima, and Keiji Tanaka
  • Journal Title: Chemistry Letters Volume: 44 Issue: 2 Pages: 166-168
  • DOI: 10.1246/cl.140924
  • NAID: 130004868347
  • ISSN: 0366-7022, 1348-0715
  • Peer Reviewed / Open Access
• [Journal Article] An Effect of Molecular Motion on Carrier Formation in a Poly(3-hexylthiophene) Film (2015)
  • Author(s): Y. Ogata, D. Kawaguchi, K. Tanaka
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 8436-8436
  • DOI: 10.1038/srep08436
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of Local Chain Dynamics on a Bioinert Interface (2015)
  • Author(s): T. Hirata, H. Matsuno, D. Kawaguchi, T. Hirai, N. L. Yamada, M. Tanaka,
  • Journal Title: Langmuir Volume: 31 Issue: 12 Pages: 3661-3667
  • DOI: 10.1021/acs.langmuir.5b00258
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Aggregation States of Polystyrene at Nonsolvent Interfaces (2014)
  • Author(s): A. Horinouchi, N. L. Yamada, K. Tanaka
  • Journal Title: Langmuir Volume: 30 Issue: 22 Pages: 6565-6570
  • DOI: 10.1021/la500829p
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Precise Synthesis and Surface Wettability of a Polymer with Liquid Crystalline Side Chains (2014)
  • Author(s): T. Hirai, S. Osumi, H. Ogawa, T. Hayakawa, A. Takahara, K. Tanaka
  • Journal Title: Macromolecules Volume: 47 Issue: 15 Pages: 4901-4907
  • DOI: 10.1021/ma5010265
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Facile Microcapsule Fabrication by Spray Deposition of a Supramolecular Hydrogel (2014)
  • Author(s): A. Shundo, Y. Hoshino, T. Higuchi, Y. Matsumoto, D. P. Penaloza, K. Matsumoto, M. Ohno, K. Miyaji, M. Goto, K. Tanaka
  • Journal Title: RSC Advances Volume: 4 Issue: 68 Pages: 36097-36100
  • DOI: 10.1039/c4ra04636f
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Simple Surface Treatment of Cell-Culture Scaffolds with Ultrafine Bubble Water (2014)
  • Author(s): H. Matsuno, T. Ohta, A. Shundo, Y. Fukunaga, K. Tanaka
  • Journal Title: Langmuir Volume: 30 Issue: 50 Pages: 15238-15243
  • DOI: 10.1021/la5035883
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of side-chain carbonyl groups on the interface of vinyl polymers with water (2014)
  • Author(s): Yukari Oda, Ayanobu Horinouchi, Daisuke Kawaguchi, Hisao Matsuno, Shokyoku Kanaoka, Sadahito Aoshima, Keiji Tanaka
  • Journal Title: Langmuir Volume: 30 Issue: 5 Pages: 1215-1219
  • DOI: 10.1021/la404802j
  • Peer Reviewed
• [Journal Article] Green Solid Films with Tunable Mechanical Properties Made from Deoxyribonucleic Acid (2014)
  • Author(s): J. Zhan, H. Matsuno, H. Masunaga, H. Ogawa, K. Tanaka
  • Journal Title: NPG Asia Materials Volume: 6 Issue: 3 Pages: e92-e92
  • DOI: 10.1038/am.2014.4
  • Peer Reviewed
• [Presentation] Effect of Mechanical Instability at Polymer Surface on Cell Adhesion (2014)
  • Author(s): Keiji Tanaka
  • Organizer: The 8th International Conference on Materials Science and Technology (MSAT-8)
  • Place of Presentation: Swissotel Le Concorde, Bangkok, Thailand
  • Year and Date: 2014-12-15 – 2014-12-16
  • Invited
• [Presentation] Physical Properties of Polymer Solids: from bulk to surface (2014)
  • Author(s): Keiji Tanaka
  • Organizer: 9th Joint Conference on Chemistry (9th JCC)
  • Place of Presentation: Grand Candi Hotel, Semarang, Indonesia
  • Year and Date: 2014-11-12 – 2014-11-13
  • Invited
• [Presentation] The Hierarchical Heterogeneity of Soft Materials (2014)
  • Author(s): Keiji Tanaka
  • Organizer: Korea-Japan Joint Polymer Symposium (KJJS2014)
  • Place of Presentation: KAIST, Daejeon, Korea
  • Year and Date: 2014-10-29 – 2014-10-31
  • Invited
• [Presentation] 元素ブロック高分子の界面構造・ダイナミクス (2014)
  • Author(s): 田中敬二
  • Organizer: 日本セラミックス協会第27回秋季シンポジウム
  • Place of Presentation: 鹿児島大学、鹿児島市
  • Year and Date: 2014-09-09 – 2014-09-11
  • Invited
• [Presentation] Structure and Dynamics of a Highly Bio‐inert Polymer at Water Interface (2014)
  • Author(s): Keiji Tanaka
  • Organizer: The 11th International Symposium on Polymer Physics (PP’2014)
  • Place of Presentation: International Conference Hotel, Nanjing, China
  • Year and Date: 2014-06-08 – 2014-06-12
  • Invited
• [Presentation] Surface Reorganization in Polymer Blend Films (2014)
  • Author(s): Keiji Tanaka
  • Organizer: The 6th Taiwan-Japan Joint Meeting on Neutron and X-ray Scattering
  • Place of Presentation: Taipei, Taiwan
  • Invited
• [Presentation] Analysis of Structure and Dynamics of Polymers by Interfacial-selective Spectroscopy (2013)
  • Author(s): Keiji Tanaka
  • Organizer: International Symposium for the 70th Anniversary of the Tohoku Branch of the Chemical Society of Japan
  • Place of Presentation: Tohoku University, Sendai, Japan
  • Invited
• [Presentation] Heterogeneity in Soft Materials (2013)
  • Author(s): Keiji Tanaka
  • Organizer: 2013 POSTECH PolymerSymposium
  • Place of Presentation: POSTECH, Pohang, Korea
  • Invited
• [Presentation] Local Conformation and Peculiar Dynamics of Polymers at Interfaces (2013)
  • Author(s): Keiji Tanaka
  • Organizer: The 13th Pacific Polymer Conference
  • Place of Presentation: Grand Hi-Lai Arena, Kaohsiung, Taiwan
  • Invited
• [Presentation] 界面制御による高分子材料の機能化 (2013)
  • Author(s): 田中敬二
  • Organizer: 第22回ポリマー材料フォーラム
  • Place of Presentation: タワーホール船堀、東京都江戸川区
  • Invited
• [Remarks]
  • URL: http://www.cstf.kyushu-u.ac.jp/~tanaka-lab/