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クライオ顕微質量分析によるリグニン生合成前駆物質の輸送と貯蔵の可視化

Publicly Offered Research

Project AreaPlant cell wall as information-processing system
Project/Area Number 25114508
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University

Principal Investigator

福島 和彦  名古屋大学, 生命農学研究科, 教授 (80222256)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords細胞壁
Outline of Annual Research Achievements

直径10 mm程度の凍結試料を用いて、高速液体クロマトグラフィーおよびイオンクロマトグラフィーによるリグニン前駆体および糖類の定量分析、樹脂法埋試料作製ならびに薄切片の紫外線顕微鏡・偏光顕微鏡・染色切片の顕微鏡観察、そして低温二次イオン質量分析/走査電子顕微鏡(cryo-TOF-SIMS/SEM)分析による各種生体成分のケミカルマッピングを行った。
急速凍結されたイチョウの木口面および柾目面について、cryo-TOF-SIMS/SEM分析を行った。形成層から木部側に向かって1細胞ずつ、その細胞断面内におけるマススペクトルを抽出した。得られた各マススペクトルからコニフェリン、スクロース、カリウムのイオン量を評価した。細胞の木化段階については紫外線顕微鏡・偏光顕微鏡・光学顕微鏡・cryo-SEM観察結果を比較することで同定した。
結果より、コニフェリン由来フラグメントイオンは、分化中木部において徐々に貯蔵量が増大し、複合細胞間層木化開始時に少し減少したが、その後も貯蔵される傾向にあった。また二次壁の木化開始と同時に急激に減少することが示唆された。放射性同位体ラベルされたコニフェリンの投与実験に関する過去の研究では、複合細胞間層形成期および二次壁形成期の細胞においてリグニンへのコニフェリン取り込みが起こると報告されている。本研究と既報の結果より、分化中木部の細胞内におけるコニフェリンの貯蔵量推移およびリグニンへの取り込み時期は、細胞壁の木化過程とよく一致しており、コニフェリンがリグニン前駆体の貯蔵・輸送形態であることを示唆した。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (24 results)

All 2015 2014 2013

All Journal Article (11 results) (of which Acknowledgement Compliant: 2 results,  Peer Reviewed: 9 results) Presentation (12 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] 最新表面分析による木材成分の可視化 ―法隆寺ヒノキ古材の物質分布を探る―2015

    • Author(s)
      齋藤香織, 福島和彦
    • Journal Title

      森林技術

      Volume: 877 Pages: 23-27

    • Related Report
      2014 Annual Research Report
  • [Journal Article] イメージング質量分析による植物細胞壁木化過程の研究 (Studies on the plant cell wall lignification by imaging mass spectrometry)2015

    • Author(s)
      青木弾、福島和彦
    • Journal Title

      植物の生長調節

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] TOF-SIMSによる植物生体分子のイメージング (TOF-SIMS Imaging of Biological Molecules in Plants)2014

    • Author(s)
      青木弾、松下泰幸、福島和彦
    • Journal Title

      BIO INDUSTRY

      Volume: 31 Pages: 29-34

    • Related Report
      2014 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] The accumulation pattern of ferruginol in the heartwood-forming Cryptomeria japonica xylem as determined by time-of-flight secondary ion mass spectrometry and quantity analysis2014

    • Author(s)
      Katsushi Kuroda, Takeshi Fujiwara, Koh Hashida, Takanori Imai, Masayoshi Kushi, Kaori Saito, Kazuhiko Fukushima
    • Journal Title

      Annals of Botany

      Volume: 113 Issue: 6 Pages: 1029-1036

    • DOI

      10.1093/aob/mcu028

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part I2014

    • Author(s)
      P. Zheng, D. Aoki, M. Yoshida, Y. Matsushita, T. Imai, K. Fukushima
    • Journal Title

      Microscopic investigation by POM, UV microscopy, and TOF-SIMS. Holzforschung

      Volume: 68 Issue: 8 Pages: 897-905

    • DOI

      10.1515/hf-2013-0231

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part II: Microchemical analysis by laser microdissection and thioacidolysis2014

    • Author(s)
      P. Zheng, D. Aoki, Y. Matsushita, S. Yagami, K. Fukushima
    • Journal Title

      Holzforschung

      Volume: 68 Issue: 8 Pages: 907-913

    • DOI

      10.1515/hf-2013-0232

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adsorption behavior of poly(dimethyl-diallylammonium chloride) on pulp fiber studied by cryo time-of-flight secondary ion mass spectrometry and cryo-scanning electron microscopy2014

    • Author(s)
      T. Masumi, Y. Matsushita, D. Aoki, R. Takama, K. Saito, K. Kuroda, K. Fukushima
    • Journal Title

      Appl. Surf. Sci.

      Volume: 289 Pages: 155-159

    • DOI

      10.1016/j.apsusc.2013.10.125

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aluminum localization in the cell walls of the mature xylem of maple tree detected by elemental imaging using time-of-flight secondary ion mass spectrometry (TOF-SIMS)2013

    • Author(s)
      K. Saito
    • Journal Title

      Holzforschung

      Volume: 0 Issue: 1 Pages: 1-8

    • DOI

      10.1515/hf-2012-0215

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Topochemical analysis of organic/inorganic components in frozen samples by cryo-TOF-SIMS/SEM system2013

    • Author(s)
      D. Aoki, K. Fukushima
    • Journal Title

      Cellulose Commun.

      Volume: 20 Pages: 188-192

    • NAID

      40019903760

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of Inorganic Elements in Heartwood and Sapwood of Hinoki Cypress Detected by TOF-SIMS2013

    • Author(s)
      K. Saito, T. Mitsutani, Y. Matsushita, T. Imai, K. Fukushima
    • Journal Title

      Mokuzai Gakkaishi

      Volume: 59 Issue: 6 Pages: 353-360

    • DOI

      10.2488/jwrs.59.353

    • NAID

      130004469051

    • ISSN
      0021-4795, 1880-7577
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of the Cryo-TOF-SIMS/SEM System and Application for Topochemical Analysis of Plant Biomolecules2013

    • Author(s)
      D. Aoki, K. Kuroda, Y. Hanaya, K. Saito, R. Takama, Y. Matsushita, K. Fukushima
    • Journal Title

      Mokuzai Gakkaishi

      Volume: 59 Issue: 6 Pages: 367-374

    • DOI

      10.2488/jwrs.59.367

    • NAID

      130004469053

    • ISSN
      0021-4795, 1880-7577
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] p-ヒドロキシフェニル核の存在とリグニン分子構造との関係2015

    • Author(s)
      野村健太、寺島典二、松下泰幸、青木弾、西村裕志、渡辺隆司、片平正人、福島和彦
    • Organizer
      第65回日本木材学会大会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Lignification of ray parenchyma cells in the xylem of Phellodendron amurense detected by LMD and TOF-SIMS2015

    • Author(s)
      Peiming Zheng, Dan Aoki, Yasuyuki Matsushita, Sachie Yagami, Yuzou Sano and Kazuhiko Fukushima
    • Organizer
      International Symposium on Wood Science and Technology (IAWPS) 2015
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-03-15 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Chemical mapping of organic/inorganic chemicals in freeze-fixed Ginkgo biloba by the cryo-TOF-SIMS/SEM system2015

    • Author(s)
      Yuto Hanaya, Dan Aoki, Yasuyuki Matsushita, Masato Yoshida, Katsushi Kuroda, Ruka Takama, and Kazuhiko Fukushima
    • Organizer
      International Symposium on Wood Science and Technology (IAWPS) 2015
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-03-15 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Studies on Enzymatic Dehydrogenative Polymerization of Monolignol Dimers2015

    • Author(s)
      Chisato Ko, Yasuyuki Matsushita, Sachie Yagami, Dan Aoki, Kazuhiko Fukushima
    • Organizer
      International Symposium on Wood Science and Technology (IAWPS) 2015
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-03-15 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Chemical mapping of plant biomolecules by the cryo-TOF-SIMS/SEM system2014

    • Author(s)
      Yuto Hanaya, Dan Aoki, Ruka Takama, Yasuyuki Matsushita, Katsushi Kuroda, and Kazuhiko Fukushima
    • Organizer
      International Conference on Polyphenols 2014
    • Place of Presentation
      名古屋大学(名古屋市千種区)
    • Year and Date
      2014-09-02 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Distribution of lignin in ray parenchyma cells from sapwood to heartwood of Pinus densiflora detected by means of TOF-SIMS and LMD2014

    • Author(s)
      Peiming Zheng, Dan Aoki, Yasuyuki Matsushita, Sachie Yagami, Kazuhiko Fukushima
    • Organizer
      International Conference on Polyphenols 2014
    • Place of Presentation
      名古屋大学(名古屋市千種区)
    • Year and Date
      2014-09-02 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of Detergents on Pulping Process2014

    • Author(s)
      Ryutaro Asai, Dan Aoki, Masao Ishiguro, Yasuyuki Matsushita, Sachie Yagami and Kazuhiko Fukushima
    • Organizer
      International Conference on Polyphenols 2014
    • Place of Presentation
      名古屋大学(名古屋市千種区)
    • Year and Date
      2014-09-02 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Distribution of lignin in ray parenchyma cells from the sapwood to heartwood of Pinus densiflora2014

    • Author(s)
      P. Zheng, D. Aoki, Y. Matsushita, S. Yagami, K. Fukushima
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] パルプ洗浄工程における洗浄剤の効果2014

    • Author(s)
      浅井龍太郎, 青木弾, 石黒正雄, 松下泰幸, 八神祐絵, 福島和彦
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      愛媛大学城北キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生体中微量成分の分布と存在形態解析におけるシンクロトロン光の役割2014

    • Author(s)
      福島和彦
    • Organizer
      第3回名古屋大学シンクロトロン光研究センターシンポジウム
    • Place of Presentation
      名古屋大学野依記念学術交流館
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Chemical mapping of tree biomolecules by the cryo-TOF-SIMS/SEM system2013

    • Author(s)
      Y. Hanaya, D. Aoki, R. Takama, Y. Matsushita, K. Kuroda, and K. Fukushima
    • Organizer
      PRWAC2013
    • Place of Presentation
      Nanjing, China
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Chemical mapping of plant biomolecules by the cryo-TOF-SIMS/SEM system2013

    • Author(s)
      Y. Hanaya, D. Aoki, R. Takama, Y. Matsushita, K. Kuroda, and K. Fukushima
    • Organizer
      SISS-15
    • Place of Presentation
      成蹊大学
    • Related Report
      2013 Annual Research Report
  • [Book] 第2章 5. TOF-SIMSを用いたリグニンの構造解析と分布の可視化, In: “リグニン利用の最新動向”2013

    • Author(s)
      福島和彦
    • Total Pages
      244
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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