ATP合成酵素を中心としたイオン駆動型分子モーターの普遍的作動原理の解明

• Project Area: Harmonized supramolecular machinery for motility and its diversity
• Project/Area Number: 25117505
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: 渡邉 力也 東京大学, 工学(系)研究科(研究院), 助教 (30540108)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 生体分子機械 / 膜輸送体 / イオン駆動力 / 1分子生物物理

Outline of Annual Research Achievements

本研究では、膜輸送体のための新しい計測技術を自主開発し、膜輸送体であるF型ATP合成酵素の構造変化ならびに機能であるイオン輸送の1分子計測を世界に先駆けて達成した。この技術は、膜輸送体だけでなく、生体膜上に存在する様々な膜タンパク質の計測に応用できる可能性があり、今後の膜タンパク質研究において大きく貢献することが強く期待される。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Torque transmission mechanism via DELSEED loop of F1-ATPase (2015)
  • Author(s): Watanabe R, Koyasu K, You H, Tanigawara M and Noji H
  • Journal Title: Biophysical Journal Volume: 108 Issue: 5 Pages: 1144-1152
  • DOI: 10.1016/j.bpj.2015.01.017
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of an ATP analogue, adenosine 5′-[α-thio]-triphosphate, on F1-ATPase rotary catalysis, torque generation, and inhibited intermediated formation (2015)
  • Author(s): Yukawa A, Watanabe R and Noji H
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 458 Issue: 3 Pages: 515-519
  • DOI: 10.1016/j.bbrc.2015.01.146
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Key Chemical Factors of Arginine Finger Catalysis of F1-ATPase Clarified by an Unnatural Amino Acid Mutation (2014)
  • Author(s): Yukawa A, Iino R, Watanabe R, Hayashi S and Noji H
  • Journal Title: Biochemistry Volume: 54 Issue: 2 Pages: 472-480
  • DOI: 10.1021/bi501138b
  • Peer Reviewed / Open Access
• [Journal Article] High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition (2014)
  • Author(s): Watanabe R., Soga N., Yamanaka T. & Noji H.
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 7076-7076
  • DOI: 10.1038/srep07076
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Thermodynamic analysis of F1-ATPase rotary catalysis using high-speed imaging (2014)
  • Author(s): Watanabe R, Minagawa Y and Noji H
  • Journal Title: Protein Science Volume: 23 Issue: 12 Pages: 1773-1779
  • DOI: 10.1002/pro.2559
  • Peer Reviewed / Open Access
• [Journal Article] Torque generation mechanism of F1-ATPase upon NTP binding (2014)
  • Author(s): Arai HC, Yukawa A, Iwatate JR, Kamiya M, Watanabe R, Urano Y and Noji H
  • Journal Title: Biophys J Volume: 107 Issue: 1 Pages: 156-164
  • DOI: 10.1016/j.bpj.2014.05.016
  • Peer Reviewed / Open Access
• [Journal Article] Arrayed lipid bilayer chambers allow single-molecule analysis of membrane transporter activity (2014)
  • Author(s): Watanabe R., Soga N., Fujita D., Tabata K. V., Yamauchi L., Kim S. H., Asanuma D., Kamiya M., Urano Y., Suga H. & Noji H.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4519-4519
  • DOI: 10.1038/ncomms5519
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Robustness of the Rotary Catalysis Mechanism of F1-ATPase (2014)
  • Author(s): Watanabe R, Matsukage Y, Yukawa A, Tabata KV and Noji H
  • Journal Title: J Biol Chem Volume: 289 Issue: 28 Pages: 19331-19340
  • DOI: 10.1074/jbc.m114.569905
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of the temperature-sensitive reaction of F1-ATPase by using single molecule manipulation (2014)
  • Author(s): Watanabe R and Noji H
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 4962-4962
  • DOI: 10.1038/srep04962
  • Peer Reviewed / Open Access
• [Journal Article] Timing of inorganic phosphate release modulates the catalytic activity of ATP-driven rotary motor protein (2014)
  • Author(s): Watanabe R and Noji H
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 3486-3486
  • DOI: 10.1038/ncomms4486
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Catalysis-enhancement via rotary fluctuation of F1-ATPase (2013)
  • Author(s): Watanabe R, Hayashi K, Ueno H and Noji H
  • Journal Title: Biophys. J Volume: 105 Issue: 10 Pages: 2385-2391
  • DOI: 10.1016/j.bpj.2013.09.050
  • Peer Reviewed
• [Journal Article] High throughput formation of sub-million lipid membrane arrays for measuring membrane protein activities (2013)
  • Author(s): Watanabe, R., Fujita, D., Tabata, KV., Yamauchi, L., Soga, N., Kim, SH., Suga, H & Noji, H.
  • Journal Title: Proceedings of 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences Volume: 1 Pages: 1314-1316
  • Peer Reviewed
• [Presentation] High throughput formation of sub-million lipid membrane arrays for measuring membrane protein activities
  • Author(s): Watanabe, R., Fujita, D., Tabata, KV., Yamauchi, L., Soga, N., Kim, SH., Suga, H & Noji, H.
  • Organizer: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Freiburg, Germany
• [Presentation] Single molecule biophysics of FoF1-ATP synthase
  • Author(s): Watanabe, R.
  • Organizer: Department Seminar. Max-Planck-Institute, Biophysics
  • Place of Presentation: Frankfurt, Germany
  • Invited
• [Presentation] Robustness of rotary catalysis mechanism of F1-ATPase
  • Author(s): Watanabe, R, & Noji, H
  • Organizer: 57th Annual meeting of Biophysical society
  • Place of Presentation: San Francisco, USA
• [Presentation] 脂質膜アレイを利用した膜輸送体の物質輸送の1分子計測
  • Author(s): 渡邉力也、曽我直樹、野地博行
  • Organizer: 日本生体エネルギー研究会年会
  • Place of Presentation: グランシップ・静岡