大脳皮質抑制性介在ニューロン発生・分化の初期過程

• Project Area: Neural Diversity and Neocortical Organization
• Project/Area Number: 25123710
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: 村上 富士夫 大阪大学, 生命機能研究科, 教授 (20089882)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 大脳皮質介在ニューロン / アストロサイト / Nkx2.1 / トランスポゼース / 大脳皮質抑制性ニューロン / 多様性 / 子宮内電気穿孔法

Outline of Annual Research Achievements

昨年度に引き続き、大脳基底核原基からの前脳腹側の様々な領域に分布するアストロサイトが産生されることを見いだした。この領域からは神経細胞も賛成されるため両者の関係に関して更なる解析を行った。具体的には特定の領域の神経細胞とアストロサイトが共通のprogenitor domain（PD）に由来するか否かを検討した。そのため、電気穿孔法を用いて大脳基底核原基にトランスポゼースをコードした遺伝子とGFPをコードする遺伝子を導入した。又一部の実験ではNkx2.1cre:Ai9マウスにこれを導入することでNkx2.1陽性領域（主に内側基底核原基）から産生される細胞を特異的に標識した。その結果、線条体腹側部や梨状葉では、赤色に標識されるNkx2.1陽性領域由来の神経細胞が緑色のみで標識されるNkx2.1陰性領域由来のアストロサイトと密接に接触している様子を確認することが出来た。脊髄では共通のPD由来のニューロンとアストロサイトに機能的連関があるが、前脳では異なるPD由来のニューロンとアストロサイトにも機能的連関があることを示唆している。異なるドメイン由来のアストロサイトにも間興味深い結果が得られ、これらはお互いの領域を避けるかのように重なり合わずに分布していた。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Evidence That the Laminar Fate of LGE/CGE-Derived Neocortical Interneurons Is Dependent on Their Progenitor Domains. (2016)
  • Author(s): Makio Torigoe, Kenta Yamauchi, Toshiya Kimura, Yo Uemura, Fujio Murakami
  • Journal Title: The Journal of neuroscience Volume: 36 Issue: 6 Pages: 2044-2056
  • DOI: 10.1523/jneurosci.3550-15.2016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] From migration to settlement: the pathways, migration modes and dynamics of neurons in the developing brain (2016)
  • Author(s): Yumiko Hatanaka, Yan Zhu, Makio Torigoe, Yoshiaki Kita, Fujio Murakami
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 92 Issue: 1 Pages: 1-19
  • DOI: 10.2183/pjab.92.1
  • NAID: 130005118553
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Chemokine Signaling Controls Integrity of Radial Glial Scaffold in Developing Spinal Cord and Consequential Proper Position of Boundary Cap Cells. (2016)
  • Author(s): Yan Zhu, Tomoko Matsumoto, Takashi Nagasawa, Fabienne Mackay, Fujio Murakami
  • Journal Title: The Journal of neuroscience Volume: 35 Issue: 24 Pages: 9211-9224
  • DOI: 10.1523/jneurosci.0156-15.2015
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium. (2015)
  • Author(s): Makio Torigoe, Kenta Yamauchi, Yan Zhu, Hiroaki Kobayashi, Fujio Murakami
  • Journal Title: Scientific reports Volume: 5 Issue: 1 Pages: 07-07
  • DOI: 10.1038/srep12258
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Calm1 signaling pathway is essential for the migration of mouse precerebellar neurons (2015)
  • Author(s): Hiroaki Kobayashi, Shunsuke Saragai, Atsushi Naito, Koji Ichio, Daisuke Kawauchi, Fujio Murakami
  • Journal Title: Development Volume: 142 Pages: 375-384
  • DOI: 10.1242/dev.112680
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Specific labeling of climbing fibers shows early synaptic interactions with immature Purkinje cells in the prenatal cerebellum. (2014)
  • Author(s): Yoshiaki Kita, Kazuto Tanaka, Fujio Murakami
  • Journal Title: Dev Neurobiol Volume: 75 Issue: 9 Pages: 927-934
  • DOI: 10.1002/dneu.22259
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evidence that dendritic mitochondria negatively regulate dendritic branching in pyramidal neurons in the neocortex. (2014)
  • Author(s): Toshiya Kimura, Fujio Murakami
  • Journal Title: The Journal of neuroscience Volume: 34 Issue: 20 Pages: 6938-6951
  • DOI: 10.1523/jneurosci.5095-13.2014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The control of precerebellar neuron migration by RNA-binding protein Csde1. (2013)
  • Author(s): H Kobayashi, D Kawauchi, Y Hashimoto, T Ogata, F Murakami
  • Journal Title: Neuroscience Volume: 253 Pages: 292-303
  • DOI: 10.1016/j.neuroscience.2013.08.055
  • Peer Reviewed
• [Journal Article] N-Cadherin Sustains Motility and Polarity of Future Cortical Interneurons during Tangential Migration (2013)
  • Author(s): Camilla Luccardini, Laetitia Hennekinne, Lucie Viou, Mitsutoshi Yanagida, Fujio Murakami, Nicoletta Kessaris, Xufei Ma, Robert S Adelstein, René-Marc Mège, Christine Métin
  • Journal Title: J. Neurosci Volume: 33 Issue: 46 Pages: 18149-60
  • DOI: 10.1523/jneurosci.0593-13.2013
  • Peer Reviewed
• [Journal Article] Development of Cerebellar Neurons and Glias Revealed by in Utero Electroporation: Golgi-Like Labeling of Cerebellar Neurons and Glias (2013)
  • Author(s): Kita, Y., Kawakami, K., Takahashi, Y. and *Murakami, F.
  • Journal Title: PLoS ONE Volume: 8 (7) Issue: 7 Pages: e70091-e70091
  • DOI: 10.1371/journal.pone.0070091
  • Peer Reviewed / Open Access
• [Journal Article] Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain (2013)
  • Author(s): Masaki Shinohara, Yan Zhu, Fujio Murakami
  • Journal Title: The Journal of comparative neurology Volume: 521 Issue: 14 Pages: 3340-57
  • DOI: 10.1002/cne.23353
  • Peer Reviewed
• [Journal Article] Role of neuropilin-2 in the ipsilateral growth of midbrain dopaminergic axons (2013)
  • Author(s): Makio Torigoe, Kenta Yamauchi, Atsushi Tamada, Ikuo Matsuda, Atsu Aiba, Valérie Castellani, Fujio Murakami
  • Journal Title: Eur J Neurosci. Volume: 37 Issue: 10 Pages: 1573-83
  • DOI: 10.1111/ejn.12190
  • Peer Reviewed