錐体細胞多様性と皮質領野間結合構築

• Project Area: Neural Diversity and Neocortical Organization
• Project/Area Number: 25123723
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: 川口 泰雄 生理学研究所, 基盤神経科学研究領域, 教授 (40169694)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 前頭皮質 / 皮質間結合 / 局所回路 / 錐体細胞 / 橋核投射細胞 / 線条体投射細胞 / トップダウン投射 / ボトムアップ投射 / 運動野 / 第５層 / 橋核投射 / 対側皮質投射 / 同側皮質投射 / Ctip2

Outline of Annual Research Achievements

これまでに前頭皮質の錐体細胞を、皮質下構造・対側皮質への投射、樹状突起形態、分子発現、発火パターンで分類し、それらの間にある結合関係を解析してきた。しかし、このようにして同定した錐体細胞が同側皮質結合にどのように関与するかは不明であった。そこで錐体細胞の近接・遠隔皮質投射先と、そこでの軸索終末の層分布を解析した。
遠隔皮質との結合では、片方の領野からもう一方への投射細胞が2/3層にある場合、別方向の投射細胞は5層に見られた。一方、近接皮質との結合では、投射細胞はどちらの領野でも2/3層、5層の両方に分布していた。軸索終末分布も投射先、特に1層・2/3層で異なっていた。これらの投射細胞・軸索終末の層分布様式から、皮質間投射のトップダウン・ボトムアップ方向を推定した。
前頭皮質5層の錐体細胞には、二種類の主要な皮質下投射サブタイプ、交叉性線条体投射細胞と橋核投射細胞があり、それらの間には、前者から後者へのシナプス結合階層性が見られる。どちらの錐体細胞も同側皮質へ軸索を出していたが、その投射様式は異なっていた。交叉性線条体投射細胞が近接・遠隔両方の皮質間投射をしていた一方、橋核投射細胞は主に近接領野、特にトップダウン方向へ投射していた。この二つのサブタイプでは、投射先での神経支配層も違っていた。交叉性線条体投射細胞は１層下部(1b層)・2/3層へ、一方、橋核投射細胞は１層上部(1a層)へより多くの軸索を出していた。これらの観察から、局所結合階層性と領野間結合階層性との関連を初めて明らかにすることができた。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Multiple layer 5 pyramidal cell subtypes relay cortical feedback from secondary to primary motor areas in rats. (2013)
  • Author(s): 植田 禎史
  • Journal Title: Cerebral Cortex Volume: - Issue: 9 Pages: 1-15
  • DOI: 10.1093/cercor/bht088
  • Peer Reviewed
• [Journal Article] Dopaminergic control of motivation and reinforcement learning: a closed-circuit account for reward-oriented behavior (2013)
  • Author(s): Kenji Morita, Mieko Morishima, Katsuyuki Sakai, Yasuo Kawaguchi
  • Journal Title: The Journal of Neuroscience Volume: 33 Issue: 20 Pages: 8866-8890
  • DOI: 10.1523/jneurosci.4614-12.2013
  • Peer Reviewed
• [Journal Article] Functional Significance of Rall's Power of Three Halves Law in Cortical Nonpyramidal Cells (2013)
  • Author(s): Yoshiyuki Kubota
  • Journal Title: Advances in Cognitive Neurodynamics (III) Volume: XVI Pages: 45-51
  • DOI: 10.1007/978-94-007-4792-0_7
  • ISBN: 9789400747913, 9789400747920
  • Peer Reviewed
• [Journal Article] Common excitatory inputs to electrically connected cortical fast-spiking cell networks. (2013)
  • Author(s): 大塚 岳，川口 泰雄
  • Journal Title: Journal of Neurophysiology Volume: 110 Issue: 4 Pages: 795-806
  • DOI: 10.1152/jn.00071.2013
  • Peer Reviewed
• [Journal Article] Direction- and distance-dependent interareal connectivity of pyramidal cell subpopulations in the rat frontal cortex. (2013)
  • Author(s): 植田 禎史，平井 康治，大塚 岳，川口 泰雄
  • Journal Title: Frontiers in Neural Circuits Volume: 11 Pages: 164-164
  • DOI: 10.3389/fncir.2013.00164
  • Peer Reviewed
• [Presentation] 大脳領域間結合と局所回路網の統合的解析 (2015)
  • Author(s): 川口泰雄
  • Organizer: CREST '脳神経回路の形成・動作原理の解明と制御技術の創出' 研究領域 第２回公開シンポジウム '脳神経回路研究の最前線2015'
  • Place of Presentation: JST東京本部別館 (東京都千代田区)
  • Year and Date: 2015-02-21
• [Presentation] Conserved dimensional properties of dendritic trees in cortical pyramidal cell (2014)
  • Author(s): Kubota Y, Mohamed AA, Yamaguchi N, Hatada S, Luebke J, Kawaguchi Y
  • Organizer: 44th annual meeting of the Society for Neuroscience
  • Place of Presentation: Walter E. Washington Convention Center (Washington DC, USA)
  • Year and Date: 2014-11-16
  • Int'l Joint Research
• [Presentation] 大脳皮質錐体細胞の樹状突起の形態解析 (2014)
  • Author(s): 窪田芳之, Mohamed AA, 根東覚, 野村真樹, 畑田小百合, 山口登, 川口泰雄
  • Organizer: 第10回IIRSセミナー
  • Place of Presentation: 東京大学 (東京都文京区)
  • Year and Date: 2014-11-07
• [Presentation] Cortical membrane oscillatory activities induced by light stimulations (2014)
  • Author(s): Otsuka T, Kawaguchi Y
  • Organizer: 44th annual meeting of the Society for Neuroscience
  • Place of Presentation: Walter E. Washington Convention Center (Washington DC, USA)
  • Year and Date: 2014-11-05
  • Int'l Joint Research
• [Presentation] Temporal structure of activities in cortical neuron subtypes during slow oscillations (2014)
  • Author(s): Ushimaru M, Kawaguchi Y
  • Organizer: 4th Joint CIN-NIPS Symposium
  • Place of Presentation: University of Tuebingen (Tuebingen, Germany)
  • Year and Date: 2014-10-06
  • Int'l Joint Research
• [Presentation] Conserved dimensional properties of dendritic trees in cortical pyramidal cell (2014)
  • Author(s): Mohamed AA, Yamaguchi N, Hatada S, Luebke J , Kawaguchi Y, Kubota Y
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜 (神奈川県横浜市)
  • Year and Date: 2014-09-13
• [Presentation] 自発運動時における視床から大脳皮質第１層への入力パターン (2014)
  • Author(s): 田中康代, 田中康裕, 和氣弘明, 正水芳人, 川口泰雄, 松崎政紀
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜 (神奈川県横浜市)
  • Year and Date: 2014-09-13
• [Presentation] 光刺激によって誘発される皮質回路におけるオシレーション活動 (2014)
  • Author(s): 大塚岳, 川口泰雄
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜 (神奈川県横浜市)
  • Year and Date: 2014-09-11
• [Presentation] Oscillatory activities evoked by light stimulations in cortical circuits (2014)
  • Author(s): Otsuka T, Kawaguchi Y
  • Organizer: NIPS International Workshop and Satellite Symposium of Neuroscience 2014
  • Place of Presentation: 生理学研究所 (愛知県岡崎市)
  • Year and Date: 2014-09-08
  • Int'l Joint Research
• [Presentation] Conserved dimensional properties of dendritic trees in cortical pyramidal cell (2014)
  • Author(s): Mohamed AA, Yamaguchi N, Hatada S, Luebke J, Kawaguchi Y, Kubota Y
  • Organizer: NIPS International Workshop and Satellite Symposium of Neuroscience 2014
  • Place of Presentation: 生理学研究所 (愛知県岡崎市)
  • Year and Date: 2014-09-08
  • Int'l Joint Research
• [Presentation] 大脳皮質のニューロンタイピングと結合マッピング (2014)
  • Author(s): 川口泰雄
  • Organizer: 自然科学研究機構新分野創成センターシンポジウム
  • Place of Presentation: 星陵会館ホール (東京都千代田区)
  • Year and Date: 2014-01-12
  • Invited
• [Presentation] Pyramidal and GABAergic cell subnetworks in the cortex (2013)
  • Author(s): Kawaguchi Y
  • Organizer: NEOCORTICAL ORGANIZATION 2
  • Place of Presentation: 生理学研究所 (愛知県岡崎市)
  • Year and Date: 2013-11-22
  • Invited
• [Presentation] Selective participation of corticopontine and commissural neurons in ipsilateral corticocortical projections from the rat frontal cortex (2013)
  • Author(s): Ueta Y, Kawaguchi Y
  • Organizer: Annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2013-11-09 – 2013-11-13
• [Presentation] Effects of manipulations of the basal ganglia pathways on reversal learning and time preference: predictions of the corticostriatal temporal difference (TD) hypothesis with implications for addiction (2013)
  • Author(s): Morita K, Kawaguchi Y
  • Organizer: 2013 annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2013-11-09 – 2013-11-13
• [Presentation] Differences in size of cortical synapses underlie distinct modes of inhibitory control (2013)
  • Author(s): Kubota Y, Kondo S, Nomura M, Karube F, Luebke J, Kawaguchi Y
  • Organizer: 2013 annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2013-11-09 – 2013-11-13
• [Presentation] Excitatory neurons are classified into two distinct groups according to their initial axonal direction (2013)
  • Author(s): Hatanaka Y, Yamauchi K, Namikawa T, Kawaguchi Y
  • Organizer: 2013 annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2013-11-09 – 2013-11-13
• [Presentation] Selective participation of corticopontine and commissural neurons in ipsilateral corticocortical projections from the rat frontal cortex (2013)
  • Author(s): Ueta Y, Kawaguchi Y
  • Organizer: 2013 annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2013-11-09 – 2013-11-13
• [Presentation] Locally limited conductance of IPSCs elicited by cortical fast spiking basket cell (2013)
  • Author(s): Kubota Y, Kondo S, Nomura M, Kawaguchi Y
  • Organizer: Cell Symposia "The Networked Brain"
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2013-11-07 – 2013-11-08
• [Presentation] Twenty cortical neuron types over 20 years (2013)
  • Author(s): Kawaguchi Y
  • Organizer: The 3rd NIPS-CIN Joint Neuroscience Symposium
  • Place of Presentation: 生理学研究所 (愛知県岡崎市)
  • Year and Date: 2013-10-11
  • Invited
• [Presentation] Direction- and distance-dependent interareal connectivity of pyramidal cell subpopulations (2013)
  • Author(s): Ueta Y, Hirai Y, Otsuka T, Kawaguchi Y
  • Organizer: The 3rd NIPS-CIN Joint Symposium
  • Place of Presentation: 生理学研究所 (愛知県岡崎市)
  • Year and Date: 2013-10-10 – 2013-10-11
• [Presentation] Classification of excitatory cortical neurons by their initial axonal outgrowth (2013)
  • Author(s): Hatanaka Y, Yamauchi K, Namikawa T, Kawaguchi Y
  • Organizer: The 3rd NIPS-CIN Joint Symposium
  • Place of Presentation: 生理学研究所 (愛知県岡崎市)
  • Year and Date: 2013-10-10 – 2013-10-11
• [Presentation] Involvements of Basal Ganglia Pathways in Reversal Learning and Time Preference: Predictions of the Corticostriatal Temporal Difference (TD) Hypothesis (2013)
  • Author(s): Morita K, Kawaguchi Y
  • Organizer: 2013 Annual Conference of the Society for Neuroeconomic EPFL
  • Place of Presentation: Lausanne (Switzerland)
  • Year and Date: 2013-09-27 – 2013-09-29
• [Presentation] 脳波リズムと視床-皮質サブネットワーク活動の解析 (2013)
  • Author(s): 牛丸弥香, 植田禎史, 川口泰雄
  • Organizer: 日本睡眠学会第38回定期学術集会
  • Place of Presentation: 秋田キャッスルホテル (秋田県秋田市)
  • Year and Date: 2013-06-27 – 2013-06-28
  • Invited
• [Presentation] Multiple layer 5 corticopontine and commissural neurons relay cortical feedback from secondary to primary motor cortex (2013)
  • Author(s): Ueta Y, Otsuka T, Kawaguchi Y
  • Organizer: NIPS International Workshop The sixth international Neural Microcircuit Conference "Functional Mechanism of Cortical Microcircuit"
  • Place of Presentation: 岡崎カンファレンスセンター (愛知県岡崎市)
  • Year and Date: 2013-06-24 – 2013-06-26
• [Presentation] Classification of excitatory cortical neurons by their initial axonal outgrowth (2013)
  • Author(s): Hatanaka Y, Yamauchi K, Namikawa T, Kawaguchi Y
  • Organizer: NIPS International Workshop The sixth international Neural Microcircuit Conference "Functional Mechanism of Cortical Microcircuit"
  • Place of Presentation: 岡崎カンファレンスセンター (愛知県岡崎市)
  • Year and Date: 2013-06-24 – 2013-06-26
• [Presentation] Frontal cortical neuron activity during slow oscillation (2013)
  • Author(s): Ushimaru M, Kawaguchi Y
  • Organizer: NIPS International Workshop The sixth international Neural Microcircuit Conference "Functional Mechanism of Cortical Microcircuit"
  • Place of Presentation: 岡崎カンファレンスセンター (愛知県岡崎市)
  • Year and Date: 2013-06-24 – 2013-06-26
• [Presentation] Local circuitry of layer 6 spiny neurons in rat frontal cortex (2013)
  • Author(s): Karube F, Fujiyama F, Kawaguchi Y
  • Organizer: NIPS International Workshop The sixth international Neural Microcircuit Conference "Functional Mechanism of Cortical Microcircuit"
  • Place of Presentation: 岡崎カンファレンスセンター (愛知県岡崎市)
  • Year and Date: 2013-06-24 – 2013-06-26
• [Presentation] Cortical pyramidal cell subtypes and connection specificity (2013)
  • Author(s): Kawaguchi Y
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館 (京都府京都市)
  • Year and Date: 2013-06-20 – 2013-06-23
  • Invited
• [Presentation] Neural circuit mechanism of reinforcement learning: new hypothesis on the mechanism of dopaminergic representation of reward prediction error (2013)
  • Author(s): Morita K, Morishima M, Sakai K, Kawaguchi Y
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館 (京都府京都市)
  • Year and Date: 2013-06-20 – 2013-06-23
• [Book] Cortical Development (2013)
  • Author(s): Kageyama R, Guillemot F, Osumi N, Shimazaki T, Miyoshi G, Nieto M, Shi S-H, Kawaguchi Y, Masu M, Noda M, Mishina M, Huang W-C, Yamamori T
  • Total Pages: 282
  • Publisher: Springer
• [Remarks] 生理学研究所大脳神経回路論研究部門
  • URL: http://www.nips.ac.jp/circuit/