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アゾ基の構造ダイナミクスを利用した蛍光プローブの創製

Publicly Offered Research

Project AreaScience on Function of Soft Molecular Systems by Cooperation of Theory and Experiment
Project/Area Number 26104509
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionThe University of Tokyo

Principal Investigator

花岡 健二郎  東京大学, 薬学研究科(研究院), 准教授 (70451854)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords分析科学 / 薬学 / 分子認識 / イメージング / 蛍光 / 光増感剤 / 低酸素 / 有機化学
Outline of Annual Research Achievements

生命現象を理解する上で、生きている状態のままの生体でリアルタイムかつ高い時空間分解能で生命現象を可視化することは、それらの理解の上で極めて重要である。このような観察を実現する技術として、簡便性や高い感度、高い時空間分解能から蛍光イメージングが生命科学研究において汎用されている。本研究においては、合成化学を駆使して新しい機能を持つ有機小分子の創製を行った。特に有機小分子内での分子の動きを制御することによって、光照射によって生じる「分子の励起状態」を制御し、光機能性分子(蛍光プローブや光増感剤)の開発を目指した。
生体内における異常な低酸素状態はがんや虚血性疾患などと密接に関与している。本年度は、低酸素環境を認識し発蛍光する蛍光プローブに加えて、低酸素環境で活性化し光増感能を回復する機能性光増感剤の開発を行った。このような機能性光増感剤の開発は、がん細胞に取り込ませてレーザー照射によって治療を行う光線力学療法(PDT)や、細胞死を誘導してその機能を解析するcell ablationに応用される。
これまでに開発に成功している低酸素環境を検出する蛍光プローブの分子デザインとして、アゾ基が低酸素環境下選択的に還元的開裂反応を受けること、及びアゾ化合物が一般的に無蛍光物質であることに着目した。すなわち、蛍光団の共役系にアゾ基を導入することで蛍光を消失させ、低酸素環境依存的にアゾ基が還元的開裂を受けることで蛍光が回復する低酸素感受性蛍光プローブの開発に成功している。一方、光増感剤の分子設計としては、重原子効果により高い効率で一重項酸素を生成することが知られているSe-xanthene系色素のアゾ誘導体としてdiMe SeRを開発し、それを母核としたdiMe azoSeRをデザイン・合成し、さらに生細胞への応用も行った。これら研究を通して、本「柔らかな分子系」の有用性を示すことに成功した。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (89 results)

All 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (24 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 24 results,  Open Access: 5 results,  Acknowledgement Compliant: 7 results) Presentation (58 results) (of which Int'l Joint Research: 9 results,  Invited: 14 results) Remarks (1 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 3 results)

  • [Int'l Joint Research] University of Burgundy(フランス)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Massachusetts General Hospital(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Chemical tools for the study of hydrogen sulfide (H<sub>2</sub>S) and sulfane sulfur and their applications to biological studies2016

    • Author(s)
      Yoko Takano, Kazuhito Shimamoto, Kenjiro Hanaoka
    • Journal Title

      Journal of Clinical Biochemistry and Nutrition

      Volume: 58 Issue: 1 Pages: 7-15

    • DOI

      10.3164/jcbn.15-91

    • NAID

      130005116632

    • ISSN
      0912-0009, 1880-5086
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Protein-Coupled Fluorescent Probe To Visualize Potassium Ion Transition on Cellular Membranes2016

    • Author(s)
      Tomoya Hirata, Takuya Terai, Hisao Yamamura, Manabu Shimonishi, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Yuji Imaizumi, Tetsuo Nagano, and Yasuteru Urano
    • Journal Title

      Analytical Chemistry

      Volume: 88 Issue: 5 Pages: 2693-2700

    • DOI

      10.1021/acs.analchem.5b03970

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Discovery of Pyruvylated Peptide-metabolizing Enzyme Using a Fluorescent Substrate-based Protein Discovery Technique2016

    • Author(s)
      Kentaro Yoshioka, Toru Komatsu, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, Yasuteru Urano
    • Journal Title

      Chemical Communications

      Volume: 52 Issue: 23 Pages: 4377-4380

    • DOI

      10.1039/c6cc00829a

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of NAD(P)H-dependent enzyme activity by time-domain ratiometry of terbium luminescence2016

    • Author(s)
      Takuya Terai, Hiroki Ito, Kenjiro Hanaoka, Toru Komatsu, Tasuku Ueno, Tetsuo Nagano, Yasuteru Urano
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 26 Issue: 9 Pages: 2314-2317

    • DOI

      10.1016/j.bmcl.2016.03.038

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Detection of NAD (P) H-dependent enzyme activity with dynamic luminescence quenching of terbium complexes2015

    • Author(s)
      Hiroki Ito, Takuya Terai, Kenjiro Hanaoka, Tasuku Ueno, Toru Komatsu, Tetsuo Nagano and Yasuteru Urano
    • Journal Title

      Chem. Commun.

      Volume: 51 Issue: 39 Pages: 8319-8322

    • DOI

      10.1039/c5cc01613d

    • Description
      in press
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of Chemical Equilibrium of Silicon-Substituted Fluorescein and Its Application to Develop a Scaffold for Red Fluorescent Probes2015

    • Author(s)
      K. Hirabayashi, K. Hanaoka, T. Takayanagi, Y. Toki, T. Egawa, M. Kamiya, T. Komatsu, T. Ueno, T. Terai, K. Yoshida, M. Uchiyama, T. Nagano, and Y. Urano
    • Journal Title

      Analytical Chemistry

      Volume: 87(17) Issue: 17 Pages: 9061-9069

    • DOI

      10.1021/acs.analchem.5b02331

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport2015

    • Author(s)
      Takuya Terai, Moe Kohno, Gaelle Boncompain, Shigeru Sugiyama, Nae Saito, Ryo Fujikake, Tasuku Ueno, Toru Komatsu, Kenjiro Hanaoka, Takayoshi Okabe, Yasuteru Urano, Franck Perez, and Tetsuo Nagano
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 137 Issue: 33 Pages: 10464-10467

    • DOI

      10.1021/jacs.5b05672

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of tissue-restricted bio-reaction suitable for in vivo targeting by fluorescent substrate library-based enzyme discovery2015

    • Author(s)
      Kentaro Yoshioka, Toru Komatsu, Akihiro Nakada, Jun Onagi, Yugo Kuriki, Mitsuyasu Kawaguchi, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, and Yasuteru Urano
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 137 Issue: 38 Pages: 12187-12190

    • DOI

      10.1021/jacs.5b05884

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Sensitive Bioluminogenic Probe for Imaging Highly Reactive Oxygen Species in Living Rats.2015

    • Author(s)
      Kojima R, Takakura H, Kamiya M, Kobayashi E, Komatsu T, Ueno T, Terai T, Hanaoka K, Nagano T, Urano Y.
    • Journal Title

      Angew Chem Int Ed Engl.

      Volume: 54 Issue: 49 Pages: 14768-14771

    • DOI

      10.1002/anie.201507530

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of a Series of Near-Infrared Dark Quenchers Based on Si-rhodamines and Their Application to Fluorescent Probes2015

    • Author(s)
      Takuya Myochin, Kenjiro Hanaoka, Shimpei Iwaki, Tasuku Ueno, Toru Komatsu, Takuya Terai, Tetsuo Nagano, and Yasuteru Urano
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 137 Issue: 14 Pages: 4759-4765

    • DOI

      10.1021/jacs.5b00246

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New Class of Bioluminogenic Probe Based on Bioluminescent Enzyme-Induced Electron Transfer: BioLeT.2015

    • Author(s)
      Takakura H, Kojima R, Kamiya M, Kobayashi E, Komatsu T, Ueno T, Terai T, Hanaoka K, Nagano T, Urano Y.
    • Journal Title

      J Am Chem Soc.

      Volume: 137 Issue: 12 Pages: 4010-4013

    • DOI

      10.1021/ja511014w

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gliotoxin Suppresses NF-kappaB Activation by Selectively Inhibiting Linear Ubiquitin Chain Assembly Complex (LUBAC)2015

    • Author(s)
      Hiroki Sakamoto, Shinichiro Egashira, Nae Saito, Takayoshi Kirisako, Simon Miller, Yoshiteru Sasaki, Tadahiko Matsumoto, Manabu Shimonishi, Toru Komatsu, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Hirotatsu Kojima, Takayoshi Okabe, Soichi Wakatsuki, Kazuhiro Iwai, Tetsuo Nagano
    • Journal Title

      ACS Chemical Biology

      Volume: 10 Issue: 3 Pages: 675-681

    • DOI

      10.1021/cb500653y

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of Photo-controllable Hydrogen Sulfide Donor Applicable in Live Cells2015

    • Author(s)
      Naoki Fukushima, Naoya Ieda, Mitsuyasu Kawaguchi, Kiyoshi Sasakura, Tetsuo Nagano, Kenjiro Hanaoka, Naoki Miyata, Hidehiko Nakagawa
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 25 Issue: 2 Pages: 175-178

    • DOI

      10.1016/j.bmcl.2014.11.084

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fluorescent Probes for Hydrogen Sulfide (H2S) and Sulfane Sulfur and Their Applications to Biological Studies2015

    • Author(s)
      Kazuhito Shimamoto, Kenjiro Hanaoka
    • Journal Title

      Nitric Oxide

      Volume: 46 Pages: 72-79

    • DOI

      10.1016/j.niox.2014.11.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Silicon-substituted Xanthene Dyes and Their Applications in Bioimaging2015

    • Author(s)
      Yu Kushida, Tetsuo Nagano and Kenjiro Hanaoka
    • Journal Title

      Analyst

      Volume: 140 Issue: 3 Pages: 685-695

    • DOI

      10.1039/c4an01172d

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorometric Assay of Integrin Activity with a Small-molecular Probe that Senses the Binding Site Microenvironment2014

    • Author(s)
      Toru Komatsu, Aoi Takeda, Kenjiro Hanaoka, Takuya Terai, Tasuku Ueno, Yukio Tada, Tetsuo Nagano and Yasuteru Urano
    • Journal Title

      Chem. Commun

      Volume: 50 Issue: 100 Pages: 15894-15896

    • DOI

      10.1039/c4cc05591h

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytoprotective Effects of Hydrogen Sulfide-releasing N-Methyl-D-aspartate Receptor Antagonists Mediated by Intracellular Sulfane Sulfur2014

    • Author(s)
      Eizo Marutani, Masahiro Sakaguchi, Wei Chen, Kiyoshi Sasakura, Jifeng Liu, Ming Xian, Kenjiro Hanaoka, Tetsuo Nagano, Fumito Ichinose
    • Journal Title

      MedChemComm

      Volume: 5 Issue: 10 Pages: 1577-1583

    • DOI

      10.1039/c4md00180j

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Boron Dipyrromethene as a Fluorescent Caging Group for Single-photon Uncaging with Long-wavelength Visible Light2014

    • Author(s)
      Nobuhiro Umeda, Hironori Takahashi, Mako Kamiya, Tasuku Ueno, Toru Komatsu, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, and Yasuteru Urano
    • Journal Title

      ACS Chem. Biol

      Volume: 9 Issue: 10 Pages: 2242-2246

    • DOI

      10.1021/cb500525p

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A design strategy for small molecule-based targeted MRI contrast agents: Their application for detection of atherosclerotic plaques2014

    • Author(s)
      Iwaki, S.; Hokamura, K.; Ogawa, M.; Takehara, Y.; Muramatsu, Y.; Yamane, T.; Hirabayashi, K.; Morimoto, Y.; Hagisawa, K.; Nakahara, K.; Mineno, T.; Terai, T.; Komatsu, T.; Ueno, T.; Tamura, K,; Adachi, Y.; Hirata, Y.; Arita, M.; Arai, H.; Umemura, K.; Nagano T. and Hanaoka K.
    • Journal Title

      Organic & Biomolecular Chemistry

      Volume: 12 Issue: 43 Pages: 8611

    • DOI

      10.1039/c4ob01270d

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rapidly Rendering Cells Phagocytic Through a Cell-surface Display Technique and Concurrent Rac Activation2014

    • Author(s)
      Hiroki Onuma, Toru Komatsu, Makoto Arita, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano and Takanari Inoue
    • Journal Title

      Sci. Signaling

      Volume: 7 Issue: 334

    • DOI

      10.1126/scisignal.2005123

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Practical Fluorescence Detection of Acrolein in Human Plasma via a Two-step Tethering Approach2014

    • Author(s)
      Masataka Togashi, Takuya Terai, Hirotatsu Kojima, Kenjiro Hanaoka, Kazuei Igarashi, Yasunobu Hirata, Yasuteru Urano, and Tetsuo Nagano
    • Journal Title

      Chem. Commun

      Volume: 50 Issue: 95 Pages: 14946-14948

    • DOI

      10.1039/c4cc02578d

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thermal or Mechanical Stimuli-induced Photoluminescence Color Change of Molecular Assembly Composed of An Amphiphilic Anthracene Derivative in Water2014

    • Author(s)
      Yoshimitsu Sagara, Toru Komatsu, Takuya Terai, Tasuku Ueno, Kenjiro Hanaoka, Takashi Kato, and Tetsuo Nagano
    • Journal Title

      Chem. Eur. J.

      Volume: 20 Issue: 33 Pages: 10397-10403

    • DOI

      10.1002/chem.201403025

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective Ablation of β-Galactosidase-expressing Cells with a Rationally Designed Activatable Photosensitizer2014

    • Author(s)
      Yuki Ichikawa, Mako Kamiya, Fumiaki Obata, Masayuki Miura, Takuya Terai, Toru Komatsu, Tasuku Ueno, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 53 Issue: 26 Pages: 6772-6775

    • DOI

      10.1002/anie.201403221

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate2014

    • Author(s)
      Halina Jurkowska, Heather B. Roman, Lawrence L. Hirschberger, Kiyoshi Sasakura, Tetsuo Nagano, Kenjiro Hanaoka, Jakub Krijt, Martha H. Stipanuk
    • Journal Title

      Amino Acids

      Volume: 46 Issue: 5 Pages: 1353-1365

    • DOI

      10.1007/s00726-014-1700-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 活性イオウ分子を検出する蛍光プローブの開発2016

    • Author(s)
      花岡健二郎
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] N-Ph rhodamine類の消光機構に基づくタグタンパク質検出蛍光プローブの開発2016

    • Author(s)
      岩木慎平、花岡健二郎、浦野泰照
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 非対称Siローダミン蛍光色素群の開発とレシオ型pHプローブへの応用v2016

    • Author(s)
      鏡味優、花岡健二郎、長野哲雄、浦野泰照
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 葉酸受容体発現細胞を選択的に可視化する蛍光プローブの開発2016

    • Author(s)
      沼澤宏治、花岡健二郎、長野哲雄、浦野泰照
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] GULTを介して細胞内に取り込まれる蛍光プローブの分子設計・合成2016

    • Author(s)
      高杉智博、花岡健二郎、佐々木綾子、山田勝也、浦野泰照
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Sulfane sulfurに可逆的に検出する蛍光プローブの分子設計・合成・応用2016

    • Author(s)
      髙野陽子、花岡健二郎、島本一史、浦野泰照
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] H2S選択的蛍光プローブの開発と阻害剤スクリーニングへの応用2016

    • Author(s)
      花岡健二郎
    • Organizer
      第89回 日本薬理学会年会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Design and Synthesis of Silicon-substituted Fluorophores and their Application to Fluorescent Probes for Biological Applications2016

    • Author(s)
      Kenjiro Hanaoka
    • Organizer
      The 3rd International Symposium for Young Chemists on Stimuli-Responsive Chemical Species for the Creation of Functional Molecules
    • Place of Presentation
      名古屋(名古屋大学)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 硫化水素・活性イオウ検出蛍光プローブの開発2016

    • Author(s)
      花岡健二郎
    • Organizer
      新学術領域研究「酸素生物学」第3回技術支援セミナー
    • Place of Presentation
      名古屋(名古屋大学)
    • Year and Date
      2016-01-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Development of a series of NIR dark quenchers based on Si-rhodamines and their application to fluorescence probes2015

    • Author(s)
      Kenjiro Hanaoka, Takuya Myochin, Yasuteru Urano
    • Organizer
      Pacifichem2015
    • Place of Presentation
      ホノルル(アメリカ)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of far-red to near-Infrared small-molecule fluorescence probes for monitoring dynamics of calcium ion2015

    • Author(s)
      Kenjiro Hanaoka
    • Organizer
      Pacifichem2015
    • Place of Presentation
      ホノルル(アメリカ)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of far-red to near-Infrared fluorophores and their application to fluorescence probes to detect hypoxia in vivo2015

    • Author(s)
      Kenjiro Hanaoka
    • Organizer
      Pacifichem2015
    • Place of Presentation
      ホノルル(アメリカ)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of the fluorescence quenching mechanism of N-phenyl rhodamines and its application to a fluorescence probe for HaloTag protein2015

    • Author(s)
      Shimpei Iwaki, Kejiro Hanaoka, Kengo Yoshida, Masaonbu Uchiyama, Yasuteru Urano
    • Organizer
      Pacifichem2015
    • Place of Presentation
      ホノルル(アメリカ)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of a library of asymmetric Si-rhodamine fluorophores and its application to ratiometric fluorescence probe2015

    • Author(s)
      Yu Kagami, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano
    • Organizer
      Pacifichem2015
    • Place of Presentation
      ホノルル(アメリカ)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a fluorescence probe for carbonic anhydrase IX based on twisted intramolecular charge transfer (TICT) state2015

    • Author(s)
      Shodai Takahashi, Kenjiro Hanaoka, Yasuteru Urano
    • Organizer
      Pacifichem2015
    • Place of Presentation
      ホノルル(アメリカ)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] H2S及びサルフェン硫黄検出蛍光プローブの開発とその応用2015

    • Author(s)
      花岡健二郎
    • Organizer
      Biochemistry and Molecular Biology (BMB2015)
    • Place of Presentation
      神戸市(神戸ポートアイランド(神戸ポートピアホテル、神戸国際会議場他))
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 非対称Siローダミン蛍光色素群の開発とレシオ型蛍光プローブ開発への応用2015

    • Author(s)
      鏡味優、花岡健二郎、長野哲雄、浦野泰照
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本市(熊本大学工学部 黒髪南地区キャンパス)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] N-Ph rhodamine類の消光機構の解析とHalo Tag検出蛍光プローブへの応用2015

    • Author(s)
      岩木慎平、花岡健二郎、吉田健吾、田原進也、竹内佐年、田原太平、内山真伸、浦野泰照
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本市(熊本大学工学部 黒髪南地区キャンパス)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] アゾ還元酵素(AzoR)を利用した新規レポーターシステムの構築2015

    • Author(s)
      申ナレ、花岡健二郎、朴文、宮川拓也、田之倉優、浦野泰照
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本市(熊本大学工学部 黒髪南地区キャンパス)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] N-Ph rhodamine類の消光機構の解析に基づく新たなHalo Tag検出蛍光プローブの開発2015

    • Author(s)
      岩木慎平、花岡健二郎、吉田健吾、田原進也、竹内佐年、田原太平、内山真伸、浦野泰照
    • Organizer
      第3回バイオ関連化学シンポジウム若手フォーラム
    • Place of Presentation
      熊本市(熊本大学工学部 黒髪南地区キャンパス)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] アゾ還元酵素(AzoR)を利用した新規レポーターシステムの開発2015

    • Author(s)
      申ナレ、花岡健二郎、朴文、宮川拓也、田之倉優、浦野泰照
    • Organizer
      第3回バイオ関連化学シンポジウム若手フォーラム
    • Place of Presentation
      熊本市(熊本大学工学部 黒髪南地区キャンパス)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Development of small-molecule fluorescence probes to detect hypoxia in vivo2015

    • Author(s)
      Kenjiro Hanaoka, Wen Piao, Tetsuo Nagano, Yasuteru Urano
    • Organizer
      World Molecular Imaging Congress
    • Place of Presentation
      ホノルル(アメリカ)
    • Year and Date
      2015-09-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a fluorescent probe targeting folate receptors for fluorescence lifetime imaging (FLIM)2015

    • Author(s)
      Koji Numasawa, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano
    • Organizer
      World Molecular Imaging Congress
    • Place of Presentation
      ホノルル(アメリカ)
    • Year and Date
      2015-09-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低酸素誘導性タンパク質CA IXを検出するoff/on型蛍光プローブの開発2015

    • Author(s)
      高橋翔大、花岡健二郎、浦野泰照
    • Organizer
      第13回次世代を担う若手のためのフィジカル・ファーマフォーラム
    • Place of Presentation
      長崎市(長崎ホテル清風)
    • Year and Date
      2015-08-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] GLUTを介して細胞内に取り込まれる蛍光プローブの開発2015

    • Author(s)
      高杉智博、花岡健二郎、佐々木綾子、山田勝也、浦野泰照
    • Organizer
      第13回次世代を担う若手のためのフィジカル・ファーマフォーラム
    • Place of Presentation
      長崎市(長崎ホテル清風)
    • Year and Date
      2015-08-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] N-Ph rhodamine類の消光機構の解析と蛍光プローブ開発への応用2015

    • Author(s)
      岩木慎平、花岡健二郎、吉田健吾、内山真伸、浦野泰照
    • Organizer
      生体機能関連化学部会若手の会 第27回サマースクール
    • Place of Presentation
      神戸市(神戸セミナーハウス)
    • Year and Date
      2015-07-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 非対称Siローダミン蛍光色素ライブラリーの構築とレシオ型pHプローブへの応用2015

    • Author(s)
      鏡味優、花岡健二郎、長野哲雄、浦野泰照
    • Organizer
      生体機能関連化学部会若手の会 第27回サマースクール
    • Place of Presentation
      神戸市(神戸セミナーハウス)
    • Year and Date
      2015-07-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] アゾ還元酵素(AzoR)を利用した新規レポーターシステム構築2015

    • Author(s)
      申ナレ、花岡健二郎、朴文、宮川拓也、田之倉優、浦野泰照
    • Organizer
      生体機能関連化学部会若手の会 第27回サマースクール
    • Place of Presentation
      神戸市(神戸セミナーハウス)
    • Year and Date
      2015-07-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新規蛍光団の創製から拓く蛍光プローブの開発2015

    • Author(s)
      花岡健二郎
    • Organizer
      第31回日本DDS学会学術集会
    • Place of Presentation
      東京都(京王プラザホテル)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Sulfane sulfurに可逆的に応答する蛍光プローブの開発2015

    • Author(s)
      島本一史、花岡健二郎、浦野泰照
    • Organizer
      日本ケミカルバイオロジー学会第10回年会
    • Place of Presentation
      仙台市(東北大学川内キャンパス)
    • Year and Date
      2015-06-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] N-Ph rhodamine類の消光機構をもとにした蛍光プローブの開発2015

    • Author(s)
      岩木慎平、花岡健二郎、吉田健吾、内山真伸、浦野泰照
    • Organizer
      日本ケミカルバイオロジー学会第10回年会
    • Place of Presentation
      仙台市(東北大学川内キャンパス)
    • Year and Date
      2015-06-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] ビスアニリン類の高効率合成に基づく非対称Siローダミン蛍光色素群の開発とレシオ型蛍光プローブ開発への応用2015

    • Author(s)
      鏡味優、花岡健二郎、長野哲雄、浦野泰照
    • Organizer
      日本ケミカルバイオロジー学会第10回年会
    • Place of Presentation
      仙台市(東北大学川内キャンパス)
    • Year and Date
      2015-06-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Development of fluorescence probes utilizing structural dynamics of azo group2015

    • Author(s)
      Kenjiro Hanaoka
    • Organizer
      KAKENHI International Symposium on "Studying the Function of Soft Molecular Systems"
    • Place of Presentation
      江東区(お台場)
    • Year and Date
      2015-06-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 炭酸脱水素酵素IXを検出する低分子蛍光プローブの開発2015

    • Author(s)
      高橋翔大、花岡健二郎、浦野泰照
    • Organizer
      第13回がんとハイポキシア研究会
    • Place of Presentation
      三島市(国立遺伝学研究所)
    • Year and Date
      2015-06-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] N-Ph rhodamine類の消光機構の解析と蛍光プローブへの応用2015

    • Author(s)
      岩木慎平、花岡健二郎、吉田健吾、内山真伸、浦野泰照
    • Organizer
      第10回日本分子イメージング学会学術集会
    • Place of Presentation
      江戸川区(タワーホール船堀)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 非対称Siローダミン蛍光色素ライブラリーの構築と新規レシオ型蛍光プローブ開発への応用2015

    • Author(s)
      鏡味優、花岡健二郎、長野哲雄、浦野泰照
    • Organizer
      第10回日本分子イメージング学会学術集会
    • Place of Presentation
      江戸川区(タワーホール船堀)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 低酸素環境下で誘導される炭酸脱水素酵素IXを検出する蛍光プローブの開発2015

    • Author(s)
      高橋翔大、花岡健二郎、浦野泰照
    • Organizer
      第10回日本分子イメージング学会学術集会
    • Place of Presentation
      江戸川区(タワーホール船堀)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 葉酸受容体発現細胞の可視化を目指した蛍光プローブの開発2015

    • Author(s)
      沼澤宏治、花岡健二郎、長野哲雄、浦野泰照
    • Organizer
      第10回日本分子イメージング学会学術集会
    • Place of Presentation
      江戸川区(タワーホール船堀)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] GLUTを介して細胞内に取り込まれる蛍光プローブの開発2015

    • Author(s)
      高杉智博、花岡健二郎、佐々木綾子、山田勝也、浦野泰照
    • Organizer
      第10回日本分子イメージング学会学術集会
    • Place of Presentation
      江戸川区(タワーホール船堀)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新たな近赤外光領域消光団の開発とその応用2015

    • Author(s)
      花岡健二郎、明珍琢也、浦野泰照
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸学院大学 (神戸)
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 炭酸脱水素酵素を検出するoff/on型蛍光プローブの開発2015

    • Author(s)
      髙橋翔大、花岡健二郎、浦野泰照
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸学院大学 (神戸)
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規蛍光団の創製から拓く蛍光イメージング2014

    • Author(s)
      花岡 健二郎
    • Organizer
      名古屋大学革新ナノバイオデバイス研究センターシンポジウム
    • Place of Presentation
      名古屋大学 (名古屋)
    • Year and Date
      2014-11-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of a Red Fluorescence Probe for Monitoring Dynamics of Cytoplasmic Calcium Ion2014

    • Author(s)
      Kenjiro Hanaoka, Takahiro Egawa, Kazuhisa Hirabayashi, Tetsuo Nagano, Yasuteru Urano
    • Organizer
      ICBS 2014 (3rd Annual Conference of the International Chemical Biology Society)
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2014-11-17 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規蛍光団の創製から拓く蛍光イメージング2014

    • Author(s)
      花岡 健二郎
    • Organizer
      SPI in vivo イメージングフォーラム2014
    • Place of Presentation
      コクヨホール (品川)
    • Year and Date
      2014-10-23 – 2014-10-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ケイ素置換フルオレセインの化学平衡の解析と蛍光プローブへの展開2014

    • Author(s)
      平林和久、花岡健二郎、高柳俊夫、長野哲雄、浦野泰照
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山大学津島キャンパス (岡山)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規蛍光団の創製から拓く蛍光イメージング2014

    • Author(s)
      花岡 健二郎
    • Organizer
      第二回生体分子サイエンスセミナー
    • Place of Presentation
      すずかけ台キャンパス (東京工業大学)
    • Year and Date
      2014-09-02
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of Far-red to Near-infrared Small-molecule Fluorophores and their Applications to Multicolor Imaging2014

    • Author(s)
      Kenjiro Hanaoka
    • Organizer
      China/Japan Young Chemists Forum (Molecular Imaging for Chemical Biology)
    • Place of Presentation
      Peking, China
    • Year and Date
      2014-08-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of Red Fluorescence Probe for Momitoring Dynamics of Cytoplasmic Calcium Ion2014

    • Author(s)
      Takahiro Egawa, Tetsuo Nagano, Kenjiro Hanaoka
    • Organizer
      第37回内藤コンファレンス
    • Place of Presentation
      ヒルトンニセコビレッジ (北海道)
    • Year and Date
      2014-07-15 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Novel design strategy of fluorescence probes to detect hypoxia2014

    • Author(s)
      Wen Piao
    • Organizer
      第37回内藤コンファレンス
    • Place of Presentation
      ヒルトンニセコビレッジ (北海道)
    • Year and Date
      2014-07-15 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 分子内回転を利用した近赤外消光団および蛍光プローブの開発2014

    • Author(s)
      花岡健二郎、明珍琢也、長野哲雄、浦野泰照
    • Organizer
      日本ケミカルバイオロジー学会第9回年会
    • Place of Presentation
      大阪大学豊中キャンパス (大阪府)
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] TICT状態を利用した炭酸脱水素酵素を検出する蛍光プローブの開発2014

    • Author(s)
      髙橋翔大、花岡健二郎、浦野泰照
    • Organizer
      日本ケミカルバイオロジー学会第9回年会
    • Place of Presentation
      大阪大学豊中キャンパス (大阪府)
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of Fluorescence Probes to Detect Hypoxia and Their Applications2014

    • Author(s)
      Wen Piao Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano
    • Organizer
      Gordon Conference (Bioorganic Chemistry)
    • Place of Presentation
      Boston (USA)
    • Year and Date
      2014-06-08 – 2014-06-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of a H2S Fluorescent Probe and its Application to Inhibitor Screening for H2S Producing Enzyme2014

    • Author(s)
      Kenjiro Hanaoka
    • Organizer
      Third International Conference on H2S in Biology and Medicine
    • Place of Presentation
      京都大学芝蘭会館 (京都)
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of Hypoxia-Sensitive Fluorescence Probes2014

    • Author(s)
      Wen Piao, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano
    • Organizer
      Third International Conference on H2S in Biology and Medicine
    • Place of Presentation
      京都大学芝蘭会館 (京都)
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 分子内回転を利用した近赤外消光団および近赤外蛍光プローブの開発2014

    • Author(s)
      花岡健二郎、明珍琢也、浦野泰照
    • Organizer
      第9回日本分子イメージング学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター (大阪)
    • Year and Date
      2014-05-22 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低酸素環境を検出する新規近赤外蛍光プローブの開発2014

    • Author(s)
      朴文、花岡健二郎、長野哲雄、浦野泰照
    • Organizer
      第9回日本分子イメージング学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター (大阪)
    • Year and Date
      2014-05-22 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] TICT状態機構を利用した炭酸脱水素酵素IX検出蛍光プローブの開発2014

    • Author(s)
      髙橋翔大、花岡健二郎、浦野泰照
    • Organizer
      第9回日本分子イメージング学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター (大阪)
    • Year and Date
      2014-05-22 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of Far-red to Near-Infrared Small-molecule Fluorophores and their Applications to Multicolor Imaging2014

    • Author(s)
      Kenjiro Hanaoka
    • Organizer
      EUROPT(R)ODE XII Conference on Optical Chemical Sensors & Biosensors
    • Place of Presentation
      Athens, Greece
    • Year and Date
      2014-04-13 – 2014-04-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Remarks] 東京大学大学院薬学系研究科 薬品代謝化学教室

    • URL

      http://www.f.u-tokyo.ac.jp/~taisha/

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Patent(Industrial Property Rights)] pH感受性蛍光プローブ2016

    • Inventor(s)
      花岡 健二郎、鏡味 優、長野 哲雄、浦野 泰照
    • Industrial Property Rights Holder
      国立大学法人 東京大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-01-15
    • Related Report
      2015 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] sulfane sulfur選択的蛍光プローブ2016

    • Inventor(s)
      花岡 健二郎、島本 一史、浦野 泰照、高野 陽子
    • Industrial Property Rights Holder
      国立大学法人 東京大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-01-21
    • Related Report
      2015 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] sulfane sulfur選択的蛍光プローブの開発2015

    • Inventor(s)
      花岡 健二郎、島本 一史、浦野 泰照
    • Industrial Property Rights Holder
      国立大学法人 東京大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-02-20
    • Related Report
      2014 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 炭酸脱水素酵素検出用蛍光プローブ2014

    • Inventor(s)
      花岡 健二郎、高橋 翔大、長野 哲雄、浦野 泰照
    • Industrial Property Rights Holder
      国立大学法人 東京大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-100772
    • Filing Date
      2014-05-14
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2022-01-28  

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