三次元外分泌腺組織のin vitro作成と生体組織度評価システムの構築

• Project Area: Hyper Bio Assembler for 3D Celluler Innovation
• Project/Area Number: 26106718
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Okayama University
• Principal Investigator: 松本 卓也 岡山大学, 医歯(薬)学総合研究科, 教授 (40324793)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2015: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2014: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: バイオマテリアル / バイオミメティック / バイオメカニクス / 細胞操作 / in vitro組織工学

Outline of Annual Research Achievements

唾液腺発生過程における周囲環境をハイドロゲルを元に作製したり、In vitroで機能する人工三次元組織の構築方法を創成したり、といった種々の生体組織成長制御モデルシステムを構築、利用することで、これまで知られていなかった新しい生命科学現象の発見に至った。
具体的には、マクロファージではなく、マクロファージコロニー刺激因子（MCSF）そのものが唾液腺成長に重要であるといった事実であったり、フィブロネクチン（FN）が唾液腺形態変化、特に管腔形成に重要であるなどの知見である。
また、骨組織が形成される過程の詳細検討を通し、有機基質、無機質、細胞といったそれぞれの要素における成熟度合いについて、数値化し評価するための指標づくりを行った。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Functional peptide KP24 enhances submandibular gland tissue growth in vitro (2016)
  • Author(s): Ikeda A, Taketa H, Sathi GA, Hirano Y, Iida S, Matsumoto T
  • Journal Title: Regener Ther Volume: 3 Pages: 88-96
  • DOI: 10.1016/j.reth.2016.02.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Scaffold-free fabrication of osteoinductive cellular constructs using mouse gingiva-derived induced pluripotent stem cells. (2016)
  • Author(s): Okawa H, Kayashima H, Sasaki JI, Miura J, Kamano Y, Imazato S, Yatani H, Matsumoto T, Egusa H.
  • Journal Title: Stem Cells International Volume: 2016 Issue: 1 Pages: 1-11
  • DOI: 10.1155/2016/6240794
  • Peer Reviewed / Open Access
• [Journal Article] Peptide-modified Substrate for Modulating Gland Tissue Growth and Morphology In Vitro (2015)
  • Author(s): Taketa H, Sathi GA, Farahat M, Rahman KA, Sakai T, Hirano Y, Kuboki T, Torii Y, Matsumoto T
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 11468-11468
  • DOI: 10.1038/srep11468
  • NAID: 120005697272
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Fabrication of biomimetic bone tissue using mesenchymal stem cell-derived three-dimensional constructs incorporating endothelial cells. (2015)
  • Author(s): Sasaki JI, Hashimoto M, Yamaguchi S, Itoh Y, Yoshimoto I, Matsumoto T, Imazato S.
  • Journal Title: PLoS ONE Volume: 10 Issue: 6 Pages: e0129266-e0129266
  • DOI: 10.1371/journal.pone.0129266
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Early initiation of endochondral ossification of mouse femur cultured in hydrogel with different mechanical stiffness. (2015)
  • Author(s): Sathi GA, Kenmizaki K, Yamaguchi S, Nagatsuka H, Yoshida Y, Matsugaki A, Ishimoto T, Imazato S, Nakano T, Matsumoto T.
  • Journal Title: Tissue Engineering Part C: Methods Volume: 21 Issue: 6 Pages: 567-575
  • DOI: 10.1089/ten.tec.2014.0475
  • Peer Reviewed
• [Journal Article] Novel 3D printed device for the simple preparation of hydrogel beads. (2015)
  • Author(s): Rahman KA, Sathi GA, Taketa H, Farahat, MI. Okada M, Torii Y, Matsumoto T.
  • Journal Title: 3D Printing & Additive manufacturing Volume: # Issue: 1 Pages: 5-11
  • DOI: 10.1089/3dp.2014.0023
  • Peer Reviewed
• [Presentation] 生体内石灰化を材料学的に再考する (2016)
  • Author(s): 松本卓也
  • Organizer: 医用高分子研究会
  • Place of Presentation: 東京
  • Year and Date: 2016-03-14
  • Invited
• [Presentation] Synthesized mechanical environment for understanding salivary gland tissue development (2016)
  • Author(s): Matsumoto T
  • Organizer: International Symposium 2016 on Nano- Biomaterials and Regenerative Medicine
  • Place of Presentation: 東京
  • Year and Date: 2016-03-11
  • Int'l Joint Research / Invited
• [Presentation] MSCs-assisted self-organizing SMG morphogenesis in 3D culture (2015)
  • Author(s): Farahat M, Matsumoto T
  • Organizer: TERMIS-World congress
  • Place of Presentation: ボストン, USA
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] MCSF regulates submandibular gland tissue development (2015)
  • Author(s): Gulsan SA, Matsumoto T
  • Organizer: TERMIS-World congress
  • Place of Presentation: ボストン, USA
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] Mechanically and chemically tuned environment for modulation of glandular tissue growth in vitro (2015)
  • Author(s): Matsumoto T
  • Organizer: International Symposium on Nanoarchitectonics for Mechanobiology
  • Place of Presentation: つくば
  • Year and Date: 2015-07-29
  • Int'l Joint Research / Invited
• [Presentation] Hydrogel-based biomimetic environment for understanding bone tissue formation (2014)
  • Author(s): Matsumoto T
  • Organizer: JADR
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-04 – 2014-12-05
  • Invited
• [Presentation] In vitro control of submandibular gland morphogenesis using RGD peptide modified alginate hydrogel (2014)
  • Author(s): Matsumoto T
  • Organizer: TERMIS-AP
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2014-09-25 – 2014-09-27
  • Invited
• [Book] 三次元ティッシュエンジニアリング (2015)
  • Author(s): 松本卓也、武田宏明、鳥井康弘、中野貴由
  • Total Pages: 400
  • Publisher: NTS出版
• [Book] Hyper Bio Assembler for 3D Cellular Systems (2015)
  • Author(s): Matsumoto T.
  • Publisher: Springer
• [Book] 三次元ティッシュエンジニアリング (2015)
  • Author(s): 松本卓也、武田宏明、鳥井康弘、中野貴由.
  • Total Pages: 346
  • Publisher: NTS出版
• [Book] Interface Oral Health Science (2014)
  • Author(s): Matsumoto T
  • Total Pages: 351
  • Publisher: Springer