脳神経系の形成と発達を制御する脳内環境の解明

• Project Area: Brain Environment
• Project/Area Number: 26111708
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kanazawa University
• Principal Investigator: 河崎 洋志 金沢大学, 脳・肝インターフェースメディシン研究センター, 教授 (50303904)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 脳神経科学 / 大脳皮質 / 脳神経系 / マウス / 発達

Outline of Annual Research Achievements

脳神経系の形成・発達を制御する遺伝要因と環境要因の解明は、脳神経医学の最重要課題の一つである。受精から始まる哺乳類の一生において最大の環境変化は「出生」といえるが、出生前後の脳神経系の変化には不明な点が多い。最近我々は、マウス大脳皮質での感覚地図形成が、新生仔の出生により制御されていることを見いだした。またこれまでに、出生が神経回路形成のみならず哺乳行動の発達も制御していることを見いだした。そこで本研究課題では、出生直後に生じる大脳皮質の成熟機構の解明を行った結果、大脳皮質の樹状突起の発達を制御する新たなメカニズムを見いだした。本研究の成果は、基礎神経科学のみならず臨床脳医学的な波及効果も大きい。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] University of Zurich(スイス)
• [Journal Article] Molecular investigations of development and diseases of the brain of higher mammals using the ferret (2017)
  • Author(s): Kawasaki H.
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 印刷中
  • NAID: 130006770759
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The effects of unilateral cochlear ablation on the expression of vesicular glutamate transporter 1 in the lower auditory pathway of neonatal rats (2017)
  • Author(s): Hasegawa H., Hatano M., Sugimoto H., Ito M., Kawasaki H. and Yoshizaki T.
  • Journal Title: Auris Nasus Larynx Volume: 印刷中 Issue: 6 Pages: 690-699
  • DOI: 10.1016/j.anl.2017.01.007
  • NAID: 120006027764
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Pathophysiological analyses of periventricular nodular heterotopia using gyrencephalic mammals (2017)
  • Author(s): Matsumoto N., Hoshiba Y., Morita K., Uda N., Hirota M., Minamikawa M., Ebisu H., Shinmyo Y. and Kawasaki H.
  • Journal Title: Human Molecular Genetics Volume: 26 Issue: 6 Pages: 1173-1181
  • DOI: 10.1093/hmg/ddx038
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation (2017)
  • Author(s): Shinmyo Y. and Kawasaki H.
  • Journal Title: Current Protocols in Neuroscience Volume: 79 Issue: 1
  • DOI: 10.1002/cpns.26
  • NAID: 120005764199
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cell type-specific expression of FoxP2 in the ferret and mouse retina (2017)
  • Author(s): Sato C., Iwai-Takekoshi L., Ichikawa Y. and Kawasaki H.
  • Journal Title: Neuroscience Research Volume: 117 Pages: 1-13
  • DOI: 10.1016/j.neures.2016.11.008
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Foxp2 regulates identities and projection patterns of thalamic nuclei during development (2017)
  • Author(s): Ebisu H., Iwai-Takekoshi L., Fujita-Jimbo E., Momoi T. and Kawasaki H.
  • Journal Title: Cerebral Cortex Volume: 印刷中 Pages: 3648-3659
  • DOI: 10.1093/cercor/bhw187
  • NAID: 120006334915
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Immunostaining of sulfatide-storing macrophages in gallbladder of a patient with metachromatic leukodystrophy (2016)
  • Author(s): Inoue N., Ikawa Y., Sato A., Yokoi A., Kuroda M., Nomura K., Sakai S., Tajima H., Ikeda H., Ebisu H., Kawasaki H., Ohta T. and Yachie A.
  • Journal Title: Pediatric Neurology Volume: 64 Pages: e3-e4
  • DOI: 10.1016/j.pediatrneurol.2016.08.006
  • Peer Reviewed
• [Journal Article] Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission (2016)
  • Author(s): Ghosh H., Auguadri L., Battaglia S., Simone Thirouin Z., Zemoura K., Messner S., Acuna M. A., Wildner H., Yevenes G. E., Dieter A., Kawasaki H., Hottiger M. O., Zeilhofer H. U., Fritschy J.-M. and Tyagarajan S. K.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 13365-13365
  • DOI: 10.1038/ncomms13365
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An essential role of SVZ progenitors in cortical folding in gyrencephalic mammals (2016)
  • Author(s): Toda T., Shinmyo Y., Dinh D. T. A., Masuda K. and Kawasaki H.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 29578-29578
  • DOI: 10.1038/srep29578
  • NAID: 120005850359
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sox11 balances dendritic morphogenesis with neuronal migration in the developing cerebral cortex (2016)
  • Author(s): Hoshiba Y., Toda T., Ebisu H., Wakimoto M., Yanagi S. and Kawasaki H.
  • Journal Title: Journal of Neuroscience Volume: 36 Issue: 21 Pages: 5775-5784
  • DOI: 10.1523/jneurosci.3250-15.2016
  • NAID: 120005830579
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation (2016)
  • Author(s): Shinmyo Y., Tanaka S., Tsunoda S., Hosomichi K., Tajima A. and Kawasaki H.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 20611-20611
  • DOI: 10.1038/srep20611
  • NAID: 120005764199
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Draxin from neocortical neurons controls the guidance of thalamocortical projections into the neocortex. (2015)
  • Author(s): Shinmyo Y., Riyadh M. A., Ahmed G., Naser I. B., Hossain M., Takebayashi H., Kawasaki H., Ohta K. and Tanaka H.
  • Journal Title: Nature communications Volume: 6 Issue: 1 Pages: 10232-10232
  • DOI: 10.1038/ncomms10232
  • Peer Reviewed / Open Access
• [Journal Article] Long-term two-photon calcium imaging of neuronal populations with subcellular resolution in adult non-human primates. (2015)
  • Author(s): Sadakane O., Masamizu Y., Watakabe A., Terada S., Ohtsuka M., Takaji M., Mizukami H., Ozawa K., Kawasaki H., Matsuzaki M., Yamamori T.
  • Journal Title: Cell Reports Volume: 13 Issue: 9 Pages: 1989-1999
  • DOI: 10.1016/j.celrep.2015.10.050
  • NAID: 120005829368
  • Peer Reviewed / Open Access
• [Journal Article] Pathophysiological analyses of cortical malformation using gyrencephalic mammals (2015)
  • Author(s): Masuda K., Toda T., Shinmyo Y., Ebisu H., Hoshiba Y., Wakimoto M., Ichikawa Y. and Kawasaki H.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 15370-15370
  • DOI: 10.1038/srep15370
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] in vivo two-photon imaging of dendritic spines in marmoset neocortex. (2015)
  • Author(s): Osamu Sadakane, Akiya Watakabe, Masanari Ohtsuka, Masafumi Takaji, Tetsuya Sasaki, Masatoshi Kasai, Tadashi Isa, Go Kato, Junichi Nabekura, Hiroaki Mizukami, Keiya Ozawa, Hiroshi Kawasaki, and Tetsuo Yamamori
  • Journal Title: eNeuro Volume: 2(4) Issue: 4 Pages: 1-10
  • DOI: 10.1523/eneuro.0019-15.2015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Classic cadherins mediate selective intracortical circuit formation in the mouse neocortex (2015)
  • Author(s): Wakimoto M., Sehara K., Ebisu H., Hoshiba Y., Tsunoda S., Ichikawa Y. and Kawasaki H.
  • Journal Title: Cerebral Cortex Volume: 25 Issue: 10 Pages: 3535-3546
  • DOI: 10.1093/cercor/bhu197
  • NAID: 120005704523
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Spatio-temporal regulation of the formation of the somatosensory system (2015)
  • Author(s): Kawasaki H.
  • Journal Title: Development, Growth & Differentiation Volume: 57 Issue: 3 Pages: 193-199
  • DOI: 10.1111/dgd.12208
  • NAID: 120005613962
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] フェレットを用いた高等哺乳動物の脳神経医学研究 (2015)
  • Author(s): 河崎洋志
  • Journal Title: 生体の科学 Volume: 66 Pages: 1-6
• [Journal Article] Establishment of an experimental ferret ocular hypertension model for the analysis of central visual pathway damage (2014)
  • Author(s): Fujishiro T., Kawasaki H., Aihara M., Saeki T., Yamagishi R., Atarashi T., Mayama C. and Araie M.
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 6501-6501
  • DOI: 10.1038/srep06501
  • NAID: 120005764197
  • Peer Reviewed / Open Access
• [Journal Article] Molecular investigations of the brain of higher mammals using gyrencephalic carnivore ferrets (2014)
  • Author(s): Kawasaki H.
  • Journal Title: Neuroscience Research Volume: 86 Pages: 59-65
  • DOI: 10.1016/j.neures.2014.06.006
  • NAID: 120005537870
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The development of suckling behavior of neonatal mice is regulated by birth. (2014)
  • Author(s): Toda T. and Kawasaki H.
  • Journal Title: Molecular Brain Volume: 7 Issue: 1 Pages: 8-8
  • DOI: 10.1186/1756-6606-7-8
  • Peer Reviewed / Open Access
• [Journal Article] 脳神経系の形成制御機構の解明とその医学的応用 (2014)
  • Author(s): 河崎洋志
  • Journal Title: 金沢大学十全医学会雑誌 Volume: 123 Pages: 34-34
  • Open Access
• [Presentation] Mechanisms of formation and malformation of the cerebral cortex of ferrets (2017)
  • Author(s): Yohei Shinmyo, Tomohisa Toda, Kosuke Masuda, Hiroshi Kawasaki
  • Organizer: Keystone Symposium
  • Place of Presentation: California, USA
  • Year and Date: 2017-01-08
  • Int'l Joint Research
• [Presentation] フェレットを使った脳神経系の形成と異常の解析 (2016)
  • Author(s): 河崎洋志
  • Organizer: オモロイ生き物研究会
  • Place of Presentation: 定山渓ホテル、北海道
  • Year and Date: 2016-10-22
  • Invited
• [Presentation] Genetic and environmental regulation of neonatal cortical development (2016)
  • Author(s): Hiroshi Kawasaki
  • Organizer: International Symposium: Circuit Construction in the Mammalian Brain
  • Place of Presentation: 大阪大学、大阪
  • Year and Date: 2016-08-08
  • Int'l Joint Research / Invited
• [Presentation] 脳神経系の形成機構の解析とその医学的応用 (2016)
  • Author(s): 河崎洋志
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2016-07-20
  • Invited
• [Presentation] フェレットを用いた高等哺乳動物の脳神経系の分子遺伝学的解析 (2015)
  • Author(s): 河崎洋志
  • Organizer: 第4回実験動物科学シンポジウム
  • Place of Presentation: 岡山理科大学、岡山
  • Year and Date: 2015-12-11
  • Invited
• [Presentation] The roles of birth and serotonin in neural circuit formation during development (2015)
  • Author(s): Kawasaki, H.
  • Organizer: Center for Functional Connectomics Symposium
  • Place of Presentation: Korea Institute of Science and Technology, Korea
  • Year and Date: 2015-11-16
  • Int'l Joint Research / Invited
• [Presentation] Two-photon calcium imaging using genetically-encoded calcium indicator in primate neocortex (2015)
  • Author(s): O. SADAKANE, Y. MASAMIZU, A. WATAKABE, S.-I. TERADA, M. OHTSUKA, M. TAKAJI, H. MIZUKAMI, K. OZAWA, H. KAWASAKI, M. MATSUZAKI, T. YAMAMORI
  • Organizer: Annula Meeting of the Society for Neuroscience 2015
  • Place of Presentation: McCormick Place, Chicago
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] マーモセット大脳皮質におけるGCaMPを用いた2光子カルシウムイメージング (2015)
  • Author(s): 定金理、正水芳人、渡我部昭哉、寺田晋一郎、大塚正成、高司雅史、水上浩明、小澤敬也、河崎洋志、松崎政紀、山森哲雄
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2015-07-28
• [Presentation] Sox11による大脳皮質神経細胞の成熟抑制機構の解析 (2015)
  • Author(s): 干場義生、戸田智久、河崎洋志
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2015-07-28
• [Presentation] 発生過程の視床パターン形成におけるFoxp2の機能解析 (2015)
  • Author(s): 蛭子はるか、岩井玲奈、桃井隆、河崎洋志
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2015-07-28
• [Presentation] 高等哺乳動物を用いた視覚系の分子生物学的解析 (2015)
  • Author(s): 河崎洋志
  • Organizer: 第101回富山大学眼科臨床カンファレンス
  • Place of Presentation: ANAクラウンプラザホテル富山、富山
  • Year and Date: 2015-06-18
  • Invited
• [Presentation] フェレットとマウスにおけるFoxP2陽性網膜神経節細胞の個性解析 (2015)
  • Author(s): 佐藤千尋、河崎洋志
  • Organizer: 第９２回日本生理学会大会・第１２０回日本解剖学会
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] The role of Foxp2 in the thalamic pattern formation during development (2015)
  • Author(s): Ebisu, H., Kawasaki H.
  • Organizer: 第８回神経発生討論会
  • Place of Presentation: 九州大学、博多
  • Year and Date: 2015-03-19 – 2015-03-20
• [Presentation] 大脳皮質神経細胞における樹状突起の成熟抑制機構の解析 (2015)
  • Author(s): 干場義生、河崎洋志
  • Organizer: 金沢大学脳肝インターフェースメディシン研究センターシンポジウム2015
  • Place of Presentation: KKR金沢、金沢
  • Year and Date: 2015-03-13
• [Presentation] 神経回路形成における軸索ガイダンス分子Draxinの機能 (2015)
  • Author(s): 新明洋平、河崎洋志
  • Organizer: 金沢大学脳肝インターフェースメディシン研究センターシンポジウム2015
  • Place of Presentation: KKR金沢、金沢
  • Year and Date: 2015-03-13
• [Presentation] 視床パターン形成におけるFoxp2の機能解析 (2015)
  • Author(s): 蛭子はるか、河崎洋志
  • Organizer: 金沢大学脳肝インターフェースメディシン研究センターシンポジウム2015
  • Place of Presentation: KKR金沢、金沢
  • Year and Date: 2015-03-13
• [Presentation] 感覚神経回路形成における出生の意義 (2014)
  • Author(s): 河崎洋志
  • Organizer: 生理研研究会「シナプス・神経ネットワークの機能ダイナミクス」
  • Place of Presentation: 生理学研究所、岡崎
  • Year and Date: 2014-12-02 – 2014-12-03
  • Invited
• [Presentation] 大脳皮質神経細胞の成熟抑制機構の解析 (2014)
  • Author(s): 干場義生、戸田智久、Wegner M., 田中弘文、柳茂、河崎洋志
  • Organizer: 国立遺伝学研究所研究会「哺乳類脳の機能的神経回路の構築メカニズム」
  • Place of Presentation: 国立遺伝学研究所、三島
  • Year and Date: 2014-12-01 – 2014-12-02
• [Presentation] The molecular mechanism of thalamic pattern formation during development (2014)
  • Author(s): Ebisu H., Iwai L., Momoi T., Kawasaki H.
  • Organizer: 国立遺伝学研究所研究会「哺乳類脳の機能的神経回路の構築メカニズム」
  • Place of Presentation: 国立遺伝学研究所、三島
  • Year and Date: 2014-12-01 – 2014-12-02
• [Presentation] The molecular mechanism of thalamic pattern formation during development (2014)
  • Author(s): Ebisu H., Iwai L., Momoi T., Kawasaki H.
  • Organizer: 44th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] The role of Sox11 in neuronal maturation in the mouse cerebral cortex (2014)
  • Author(s): Hoshiba Y., Toda T., Wegner M., Yanagi S., Tanaka H., Kawasaki H.
  • Organizer: 44th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] マウス大脳皮質の神経細胞成熟過程におけるSox11の機能解析 (2014)
  • Author(s): 干場義生、戸田智久、河崎洋志
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 脳神経系の形成過程における出生の役割 (2014)
  • Author(s): 河崎洋志
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2014-09-11 – 2014-09-13
  • Invited
• [Presentation] Birth regulates sensory map formation during development (2014)
  • Author(s): Hiroshi Kawasaki
  • Organizer: 第４７回日本発生生物学会
  • Place of Presentation: ウインク愛知、名古屋
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] 出生による脳神経系発達の制御メカニズム (2014)
  • Author(s): 河崎洋志
  • Organizer: 第１２回GPCR研究会
  • Place of Presentation: 日本科学未来館、東京
  • Year and Date: 2014-05-09 – 2014-05-10
  • Invited
• [Book] Brain Evolution by Design (2017)
  • Author(s): Shinmyo Y., Masuda K., Hoshiba Y., Ebisu H. and Kawasaki H.
  • Publisher: Springer Publishers
• [Book] Electroporation Methods and Neuroscience (2014)
  • Author(s): Kawasaki H. (Saito T. ed.)
  • Total Pages: 9
  • Publisher: Springer Publishers
• [Book] 脳神経系の発生・再生の融合的新展開 (2014)
  • Author(s): 河崎洋志（井村裕夫、高橋淳監修）
  • Total Pages: 6
  • Publisher: 診断と治療社
• [Book] 分子脳科学 (2014)
  • Author(s): 河崎洋志（三品昌美編）
  • Total Pages: 7
  • Publisher: 化学同人
• [Remarks] 河崎研究室
  • URL: http://square.umin.ac.jp/top/kawasaki-lab/
• [Remarks] 河崎研究室
  • URL: http://square.umin.ac.jp/top/kawasaki/Welcome.html
• [Patent(Industrial Property Rights)] 高等哺乳動物を用いた新規脳疾患モデル動物 (2015)
  • Inventor(s): 河崎洋志、桝田宏輔
  • Industrial Property Rights Holder: 河崎洋志、桝田宏輔
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-006388
  • Filing Date: 2015-01-16