植物表皮の幹細胞維持と分化の制御ロジックに関わる内的因子と新奇化合物の探索

• Project Area: Multidimensional Exploration of Logics of Plant Development
• Project/Area Number: 26113507
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University
• Principal Investigator: 鳥居 啓子 名古屋大学, トランスフォーマティブ生命分子研究所, 客員教授 (60506103)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 化合物ライブラリー / 植物 / 気孔 / バイオマス / 生長促進 / 幹細胞 / 作物 / 植物科学 / 発生・形態形成 / 化合物スクリーン / 薬理 / 国際研究者交流 / 国際情報交換

Outline of Annual Research Achievements

植物表皮の通気口である気孔は、一対の孔辺細胞が膨張・収縮することにより開閉し、 光合成のためのガス交換と蒸散を調節する。気孔の存在は植物の生長と生存に必須なだけでなく、地球レベルの大気環境に影響を及ぼしている。気孔は、気孔系譜の幹細胞であるメリステモイド細胞から生まれ、 メリステモイド細胞の数は様々な外部環境に適合するよう巧妙に調節されている。本研究では、申請者が新規に所属することになった名古屋大学トランスフォーマティブ生命分子研究所(ITbM)の理念である「合成化学と生物学の融合による新奇化合物の創出」を目指し、メリステモイド細胞の維持と分化を司る内的因子、および合成化合物の探索と同定を目的とする。

平成２７年度末までに、孔辺細胞特異的に強いGFP蛍光を発するシロイヌナズナレポーター植物を用いて現在までに約4200種類の化合物をスクリーニングした。その結果、気孔の数とメリステモイド細胞の数を増やす一群の新奇化合物が同定された。これらの中から効果が高く副作用が少ない候補をさらに選抜し、２つの化合物について詳細な解析を行った。その内の一つはメリステモイド細胞の増殖と気孔の分化の両方を著しく促進した。 驚く事に、この化合物は地上部および地下部のバイオマスを増大させた。この効果は、広く作物植物で認められたため、この化合物は気孔の分化とバイオマス生産の両方に共通するプロセスに関わることが示唆された。今後は、合成化学によるアナログ（類似化合物）のレパートリーを増やし、生理活性を担う化合物構造を狭めるとともに、標的因子の同定につなげたい。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] University of Washington/Department of Pharmacology(米国)
• [Journal Article] Lineage-specific stem cells, signals and asymmetries during stomatal development (2016)
  • Author(s): Han SK and Torii KU
  • Journal Title: Development Volume: 143 Issue: 8 Pages: 1259-1270
  • DOI: 10.1242/dev.127712
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Plant synthetic biology for molecular engineering of signalling and development (2016)
  • Author(s): Nemhauser JL, Torii KU
  • Journal Title: Nature Plants Volume: 2 Issue: 3
  • DOI: 10.1038/nplants.2016.10
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Stomagenesis versus myogenesis: Parallels in intrinsic and extrinsic regulation of transcription factor mediated specialized cell-type differentiation in plants and animals (2016)
  • Author(s): Putarjunan A, Torii KU
  • Journal Title: Development, Growth & Differentiation Volume: 58 Issue: 4 Pages: 341-354
  • DOI: 10.1111/dgd.12282
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Competitive binding of antagonistic peptides fine-tunes stomatal patterning (2015)
  • Author(s): Lee JS, Hnilova M, Maes M, Lin YC, Putarjunan A, Han SK, Avila J, Torii KU
  • Journal Title: Nature Volume: 522 Issue: 7557 Pages: 439-443
  • DOI: 10.1038/nature14561
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Framework of a Regulatory Circuit Initiating Two-Dimensional Spatial Patterning of Stomatal Lineage (2015)
  • Author(s): Horst RJ, Fujita H, Lee JS, Rychel AL, Garrick JM, Kawaguchi M, Peterson KM, Torii KU
  • Journal Title: PLoS Genetics Volume: 11
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Differential Function of Arabidopsis SERK Family Receptor-like Kinases in Stomatal Patterning. (2015)
  • Author(s): Meng X, Chen X, Mang H, Liu C, Yu X, Gao X, Torii KU, He P, Shan L
  • Journal Title: Current Biology Volume: 25 Issue: 18 Pages: 2361-2372
  • DOI: 10.1016/j.cub.2015.07.068
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cell walls as a stage for intercellular communication regulating shoot meristem development (2015)
  • Author(s): Tameshige T, Hirakawa Y, Torii KU, Uchida N
  • Journal Title: Front Plant Sci Volume: 11 Pages: 324-324
  • DOI: 10.3389/fpls.2015.00324
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Stomatal differentiation: the beginning and the end (2015)
  • Author(s): Torii, KU
  • Journal Title: Currrent Opinion in Plant Biology Volume: 28 Pages: 16-22
  • DOI: 10.1016/j.pbi.2015.08.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Co-Immunoprecipitation of Membrane-Bound Receptors (2015)
  • Author(s): Avila JR, Lee JS, Torii KU
  • Journal Title: Arabidopsis Book Volume: 13 Pages: e0180-e0180
  • DOI: 10.1199/tab.0180
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Regulatory Circuitry Initiatinve 2-Dimensional Stomatal Patterning in the Plant Epidermis (2015)
  • Author(s): Torii, K.U.
  • Organizer: BMB 2015
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Genome-Scale Mapping of Regulatory DNA Elements During Initiation, Amplification, and Differentiation of Stomatal Cell Lineages (2015)
  • Author(s): Torii, K.U.
  • Organizer: Cold Spring Harbor Asia Symposiumm “Plant Epigenetics and Development”
  • Place of Presentation: Suzhou, China
  • Year and Date: 2015-06-08
  • Int'l Joint Research / Invited
• [Presentation] Patterning Plant Epidermis: Cell Fate and Communication (2015)
  • Author(s): 3.Torii, K.U.
  • Organizer: Japanese Society of Developmental Biologists, Annual Conference
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2015-06-02
  • Invited
• [Presentation] Stomatal Patterning and Differentiaion ~Signaling Mechanism and Environmental Interface~ (2015)
  • Author(s): Keiko U. Torii
  • Organizer: 新学術領域 植物環境突破力シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2015-03-13
  • Invited
• [Presentation] Mix and Match: Receptor Kinase Complexes in Stomatal Patterning (2015)
  • Author(s): Keiko U. Torii
  • Organizer: Keystone Symposiium
  • Place of Presentation: Taos, NM, USA
  • Year and Date: 2015-02-09 – 2015-02-13
  • Invited
• [Presentation] Stomatal Patterning, Communicate, Fate, and Decision Making (2014)
  • Author(s): Keiko U. Torii
  • Organizer: International Conference of Arabidopsis Research 2014 (ICAR2014)
  • Place of Presentation: Vancouver, BC, Canada
  • Year and Date: 2014-07-28 – 2014-08-01
  • Invited
• [Presentation] Receptor Kinase Specificity and Integration in Stomatal Patterning (2014)
  • Author(s): Keiko U. Torii
  • Organizer: Gordon Research Conference
  • Place of Presentation: Holderness, NH , USA
  • Year and Date: 2014-07-20 – 2014-07-25
  • Invited
• [Presentation] Two-Dimensional Spatial Patterning in the Plant Epidermis (2014)
  • Author(s): Keiko U. Torii
  • Organizer: Annual Japanese Society of Developmental Biology Conference （日本発生学会年会）
  • Place of Presentation: 名古屋
  • Year and Date: 2014-05-27 – 2014-05-30
  • Invited
• [Remarks] ITbM press release
  • URL: http://www.itbm.nagoya-u.ac.jp/en/research/2015/06/Torii-Stomata.php
• [Remarks] UW News
  • URL: http://www.washington.edu/news/2015/06/17/plants-make-big-decisions-with-microscopic-cellular-competition/