行動の種差を規定するゲノム・遺伝子相関の解明

• Project Area: Correlative gene system: establishing next-generation genetics
• Project/Area Number: 26113702
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: 山元 大輔 東北大学, 生命科学研究科, 教授 (50318812)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2015: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000); Fiscal Year 2014: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: 神経突起形成 / 転写因子 / ショウジョウバエ / fruitless / 求愛行動 / 性的二型 / 転写標的 / 分化 / 脳・神経 / 発生・分化 / 遺伝学 / 遺伝子 / 細胞・組織

Outline of Annual Research Achievements

同種の雌雄は、次世代への遺伝情報の継承にあたって、協調的であると同時に対立的でもある。雌雄の行動上での駆け引きの背後には、神経ネットワークレベルでの雌雄差があり、その神経の性的二型はまた、遺伝子発現の性差に根差す。行動と神経の性差に対して最も大きな効果を有すると思われるのは、ショウジョウバエのfruitless (fru)遺伝子である。fru mRNAは雄の神経系でのみ翻訳されてFruタンパク質を生成する。Fruタンパク質はヒストン脱アセチル化酵素のHDAC1やヘテロクロマチンタンパク質1aと複合体を形成して染色体の標的部位に結合し、クロマチン修飾を介して一連の標的遺伝子群の転写を雄型の状態にすると考えられる。しかしこれまで、Fruの標的遺伝子は同定されていない。我々はとりわけ顕著な性的二型を示すmAL介在ニューロンに着目し、雄特異的突起の有無がrobo1遺伝子の機能に依存することを見出した。さらにゲルシフトアッセイ、レポーターアッセイによってrobo1遺伝子制御領域内にFru結合配列を同定し、robo1がFruの直接の標的であることを立証した。さらにrobo1遺伝子制御領域のFru結合配列だけを欠失した変異を作り出し、その変異体雄に固有の求愛行動の異常を見出した。この行動異常は、mAL特異的にrobo1をノックダウンすることで救済された。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Clustered regulatory interspaced short palindromic repeats (CRISPR)-mediated mutagenesis and phenotype rescue by piggyBac transgenesis in a nonmodel Drosophila species. (2016)
  • Author(s): Tanaka, R., Murakami, H., Ote, M. and Yamamoto, D.
  • Journal Title: Insect Mol. Biol. Volume: 25 Issue: 4 Pages: 12232-12232
  • DOI: 10.1111/imb.12232
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Requirement of the Tec family tyrosine kinase Btk29A for courtship memory in Drosophila males. (2016)
  • Author(s): Sunouchi, K. Koganezawa, M. and Yamamoto, D.
  • Journal Title: Arch. Insect Biochem. Physiol. Volume: 91 Issue: 3 Pages: 165-174
  • DOI: 10.1002/arch.21316
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Zeste tunes the timing of ecdysone actions in triggering programmed tissue degeneration in Drosophila (2015)
  • Author(s): Goto T., Sato K., Sone H., Koganezawa M., Ito H., Yamamoto D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 4 Pages: 169-173
  • DOI: 10.3109/01677063.2015.1098638
  • Peer Reviewed
• [Journal Article] The Dmca1D channel mediates Ca(2+) inward currents in Drosophila embryonic muscles (2015)
  • Author(s): Hara Y., Koganezawa M., Yamamoto D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 2-3 Pages: 117-123
  • DOI: 10.3109/01677063.2015.1054991
  • Peer Reviewed
• [Journal Article] Drosophila ovipositor extension in mating behavior and egg deposition involves distinct sets of brain interneurons (2015)
  • Author(s): Ken-ichi Kimura, Chiaki Sato, Masayuki Koganezawa, Daisuke Yamamoto
  • Journal Title: PLOS ONE Volume: 10 Issue: 5 Pages: e0126445-e0126445
  • DOI: 10.1371/journal.pone.0126445
  • Peer Reviewed / Open Access
• [Journal Article] フェロモンと性行動--ハエとヒトは同じか― (2015)
  • Author(s): 山元大輔
  • Journal Title: Aroma Research Volume: 16 Pages: 271-275
• [Journal Article] From the back or front: the courtship position is a matter of smell and sight in Drosophila melanogaster males. (2015)
  • Author(s): Kimura, K. I., Sato, C., Yamamoto, K., and Yamamoto, D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 1 Pages: 18-22
  • DOI: 10.3109/01677063.2014.968278
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Visually induced initiation of Drosophila innate courtship-like following pursuit is mediated by central excitatory state. (2015)
  • Author(s): Kohatsu, S. and Yamamoto D.
  • Journal Title: Nat. Commun. Volume: 6 Issue: 1 Pages: 6457-6457
  • DOI: 10.1038/ncomms7457
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Btk29A-Mediated Tyrosine Phosphorylation of Armadillo/β-Catenin Promotes Ring Canal Growth in Drosophila Oogenesis. (2015)
  • Author(s): Hamada-Kawaguchi, N. Nishida, Y., and Yamamoto D.
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: e0121484-e0121484
  • DOI: 10.1371/journal.pone.0121484
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Drosophila Lingerer protein cooperates with Orb2 in long-term memory formation. (2014)
  • Author(s): Kimura, S., Sakakibara, Y., Sato, K., Ote, M., Ito, H., Koganezawa, M., and Yamamoto, D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 1 Pages: 8-17
  • DOI: 10.3109/01677063.2014.917644
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A fruitless upstream region that defines the species specificity in the male-specific muscle patterning in Drosophila. (2014)
  • Author(s): Takayanagi, S., Toba, G., Lukacsovich, T., Ote, M., Sato, K., and Yamamoto, D.
  • Journal Title: J. Neurogenet. Volume: 29 Issue: 1 Pages: 23-29
  • DOI: 10.3109/01677063.2014.983101
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Neuroethology of male courtship in Drosophila: from the gene to behavior. (2014)
  • Author(s): Yamamoto D., Sato K., and Koganezawa M.
  • Journal Title: J. Comp. Physiol. A. Volume: 200 Issue: 4 Pages: 251-264
  • DOI: 10.1007/s00359-014-0891-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 「越境する性」の生物学 (2014)
  • Author(s): 山元大輔, 佐藤耕世.
  • Journal Title: 科学 Volume: 84 Pages: 736-744
  • Peer Reviewed
• [Journal Article] ショウジョウバエの行動遺伝学 (2014)
  • Author(s): 下山晴彦（編）, 山元大輔 (分担執筆).
  • Journal Title: 『誠信 心理学辞典 新版』誠信書房 Volume: - Pages: 792-800
  • Peer Reviewed
• [Journal Article] 生物の窓, 同性に求愛するハエ (2014)
  • Author(s): 山元大輔
  • Journal Title: 現代化学 Volume: 525 Pages: 36-38
  • NAID: 40020262934
• [Presentation] Searching for ppk-expressing neurons that control female receptivity (2016)
  • Author(s): Miwa, Y., Koganezawa, M., Emoto, K., Yamamoto, D.
  • Organizer: 第60回日本応用動物昆虫学会大会
  • Place of Presentation: 大阪市立大学、堺、大阪
  • Year and Date: 2016-03-26
• [Presentation] An analysis of a role of visual cues in the courtship induction in Drosophila subobscura. (2016)
  • Author(s): Higuchi, T., Kohatsu, S., Yamamoto, D.
  • Organizer: 第60回日本応用動物昆虫学会大会
  • Place of Presentation: 大阪市立大学、堺、大阪
  • Year and Date: 2016-03-26
• [Presentation] Drosophila subobscuraにおけるfruitless変異体の表現型解析 (2016)
  • Author(s): 田中良弥，村上日向，大手学，山元大輔．
  • Organizer: 平成28年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-26
• [Presentation] Brain insulin-producing cells integrate light and temperature information for diapause control in Drosophila (2016)
  • Author(s): Yamamoto, D.
  • Organizer: Invertebrate Neuropeptide Conference
  • Place of Presentation: Solar do Rosario Hotel, Auro Preto, Brazil
  • Year and Date: 2016-02-14
  • Int'l Joint Research / Invited
• [Presentation] 行動の種間差を生み出すメカニズムを探る～ショウジョウバエの求愛行動を用いた試み～ (2015)
  • Author(s): 田中良弥,村上 日向,大手 学,山元 大輔.
  • Organizer: 第3回生態進化発生コロキウム
  • Place of Presentation: 東京
  • Year and Date: 2015-12-28
• [Presentation] 行動の種差を規定するゲノム・遺伝子相関の解明 (2015)
  • Author(s): 山元大輔
  • Organizer: 平成２７年度新学術領域「ゲノム・遺伝子相関」班会議
  • Place of Presentation: 東京大学 弥生講堂, 東京
  • Year and Date: 2015-12-20
• [Presentation] ショウジョウバエの求愛と攻撃を切り替える神経機構 (2015)
  • Author(s): 小金澤雅之
  • Organizer: 新学術領域「適応回路シフト」第2回領域会議
  • Place of Presentation: 東京医科歯科大学, 東京
  • Year and Date: 2015-12-16
• [Presentation] Genes and circuits for male courtship behavior in Drosophila (2015)
  • Author(s): Yamamoto, D.
  • Organizer: EMBO Conference “Neural Development
  • Place of Presentation: Academia Sinica, Taipei, Taiwan
  • Year and Date: 2015-12-04
  • Int'l Joint Research / Invited
• [Presentation] Analysis of Sxl functions in germ stem cells in Drosohpila (2015)
  • Author(s): Okano, S. Ote, M. Yamamoto, D.
  • Organizer: 第38回日本分子生物学会年会第88回日本生化学大会合同大会
  • Place of Presentation: 神戸ポートアイランド, 兵庫
  • Year and Date: 2015-12-01
• [Presentation] ロイシン添加によるオートファジー不全を改善するメカニズムとSpin1との関連 (2015)
  • Author(s): 柳澤比呂子, 石井智裕, 河上江美子, 遠藤堅太郎, 平岡由佳, 上野隆, 山元大輔, 小松雅明, 渡部和彦
  • Organizer: 第38回日本分子生物学会年会第88回日本生化学大会合同大会
  • Place of Presentation: 神戸ポートアイランド, 兵庫
  • Year and Date: 2015-12-01
• [Presentation] Searching for fru-target genes that regulate the development of sexual dimorphisms in Drosophila central neurons (2015)
  • Author(s): Ito, H. Sato, K. Kondo, S. Ueda, R. Yamamoto, D.
  • Organizer: 第38回日本分子生物学会年会第88回日本生化学大会合同大会
  • Place of Presentation: 神戸ポートアイランド, 兵庫
  • Year and Date: 2015-12-01
• [Presentation] The neural basis for experience-dependent modifications of male courtship in Drosophila (2015)
  • Author(s): Yamamoto, D., Kohatsu, S.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research / Invited
• [Presentation] GABAergic neural circuitry controlling courtship and aggression in Drosophila (2015)
  • Author(s): Koganezawa, M., Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research / Invited
• [Presentation] Sex-specific cleavage of lola specifies sex-specific neurite structures in Drosohila (2015)
  • Author(s): Sato, K., Ito, H., Koganezawa, M., Toba, G., Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] earching for fru-target genes that regulate the development of sexual dimorphisms in Drosophila central neurons (2015)
  • Author(s): Ito, H. Sato, K. Kondo, S. Ueda, R. Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] Neural correlates of vision-dependent courtship following revealed by in vivo caicium imaging in Drosophila (2015)
  • Author(s): Kohatsu, S. Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] A possible role of cell-cell interactions in the sex-specific neurite patterning in Drosohiila central neurons (2015)
  • Author(s): Kato, T., Sato, K., Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] Doublesex expressing neurons that contribute to copulation in Drosophila (2015)
  • Author(s): Yilmazer, Y. Koganezawa, M. Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] DTRF2 acts as a co-factor of fruitless, a major neural masculinizing parameter in Drosophila (2015)
  • Author(s): Chowdhury, Z., Sato, K., Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] elect ppk-expressing neurons control female sexual receptivity in Drosophila (2015)
  • Author(s): Miwa, Y., Koganezawa, M., Emoto, K., Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] nalysis of visual behavior that constitutes the courtship ritual in Drosohpila subobscura (2015)
  • Author(s): Higuchi, T., Kohatsu, S., Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] Effects of social experience on inter-male courtship in Drosophila fruitless mutants (2015)
  • Author(s): Takei, T., Koganezawa, M., Yamamoto, D.
  • Organizer: NTNU-Tohoku Univ. Brain Science Meeting “Joy of Brain”
  • Place of Presentation: 東北大学, 宮城
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] Male-specific transcription factor Fruitless non-transcriptionally suppresses proteolytic cleavage of a Lola isoform to generate sexual differences in neuronal structures and behavior of Drosophila (2015)
  • Author(s): Sato, K. Ito, H. Koganezawa, M. and Yamamoto, D.
  • Organizer: Neurobiology of Drosophila Meeting at Cold Spring Harbor Laboratory, USA
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
  • Year and Date: 2015-09-29
  • Int'l Joint Research
• [Presentation] ショウジョウバエの脳の性を決める分子機構 (2015)
  • Author(s): 伊藤弘樹
  • Organizer: 日本遺伝学会第87回大会
  • Place of Presentation: 東北大学川内キャンパス, 宮城
  • Year and Date: 2015-09-24
  • Invited
• [Presentation] ショウジョウバエ雄の求愛行動を生み出す中枢神経活動のin vivo光学計測 (2015)
  • Author(s): 古波津創, 山元大輔.
  • Organizer: 日本遺伝学会第87回大会
  • Place of Presentation: 東北大学川内キャンパス, 宮城
  • Year and Date: 2015-09-24
  • Invited
• [Presentation] 非モデル種Drosophila subobscuraでのCRISPR-Cas9による変異誘発 (2015)
  • Author(s): 村上日向. 田中良弥. 伊藤弘樹. 山元大輔.
  • Organizer: 日本遺伝学会第87回大会
  • Place of Presentation: 東北大学川内キャンパス, 宮城
  • Year and Date: 2015-09-24
• [Presentation] hat are Fruitless proteins really doing for LOVEly fruit flies? (2015)
  • Author(s): Yamamoto, D.
  • Organizer: Seminars at Department of Physiology, Anatomy and Genetics Oxford University.
  • Place of Presentation: Univerisity of Oxford, UK
  • Year and Date: 2015-09-18
  • Int'l Joint Research / Invited
• [Presentation] ショウジョウバエの求愛と攻撃を解発する脳内fru/dsx発現ニューロン (2015)
  • Author(s): 小金澤雅之, 山元大輔.
  • Organizer: 第86回日本動物学会
  • Place of Presentation: 新潟コンベンションセンター, 新潟
  • Year and Date: 2015-09-16
• [Presentation] 求愛行動を規定する遺伝と環境―ショウジョウバエの場合 (2015)
  • Author(s): 山元大輔
  • Organizer: 第1042回生物科学セミナー
  • Place of Presentation: 東京大学 理学部2号館講堂. 東京
  • Year and Date: 2015-09-04
• [Presentation] 生細菌Wolbachiaによる宿主の生殖細胞操作 (2015)
  • Author(s): 大手学,山元大輔
  • Organizer: 第4回新学術領域配偶子制御領域会議
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-09-02
• [Presentation] A new experimental paradigm for the study of visually incuced courtship behavior in Drosophila species (2015)
  • Author(s): Higuchi, T. Kohatsu, S. and Yamamoto, D.
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場, 兵庫
  • Year and Date: 2015-07-28
• [Presentation] Nature vs. nurture in Drosophila courtship (2015)
  • Author(s): Yamamoto, D.
  • Organizer: EMBO-Kavli Workshop on Neural Circuits and Behaviour of Drosophila
  • Place of Presentation: Kolymbari, Crete, Greece
  • Year and Date: 2015-07-05
  • Int'l Joint Research / Invited
• [Presentation] Nature vs. Nurture in fly courtship (2015)
  • Author(s): Yamamoto, D.
  • Organizer: International Symposium of Correlative Gene System. Nara
  • Place of Presentation: Nara Prefectural New Publi Hall. Nara
  • Year and Date: 2015-05-28
  • Int'l Joint Research / Invited
• [Presentation] 性行動の神経機構―ショウジョウバエに問う”遺伝と環境” (2015)
  • Author(s): 山元大輔
  • Organizer: 第79回日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 信州大学 松本キャンパス 旭総合研究棟
  • Year and Date: 2015-05-23
  • Invited
• [Presentation] Possible involvement of neuropeptide-expressing neurons in the regulation of female receptivity (2015)
  • Author(s): Miwa, Y., Koganezawa, M., Emoto, K., Yamamoto, D.
  • Organizer: The 3rd Asia-Pacific Drosophila Research Conference.
  • Place of Presentation: Beijing Conference Center, China
  • Year and Date: 2015-05-11
  • Int'l Joint Research
• [Presentation] Studies on visually induced courtship behavior in Drosophila species by a newly established tethered male preparation (2015)
  • Author(s): Higuchi, T. Kohatsu, S. Yamamoto, D.
  • Organizer: The 3rd Asia-Pacific Drosophila Research Conference.
  • Place of Presentation: Beijing Conference Center, China
  • Year and Date: 2015-05-11
  • Int'l Joint Research
• [Presentation] Dynamic central neuron activities that underlie courtship pursuit in Drosohpila male (2015)
  • Author(s): Kohatsu, S. and Yamamoto D.
  • Organizer: 56th Annual Drosohpila Research Conference
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2015-03-04 – 2015-03-08
• [Presentation] フェロモンと性行動－ハエとヒトは同じか. (2014)
  • Author(s): 山元大輔.
  • Organizer: 第15回アロマ・サイエンス・フォーラム2014.
  • Place of Presentation: アルカディア市ヶ谷, 千代田区, 東京都
  • Year and Date: 2014-12-12
  • Invited
• [Presentation] Pheromone sensitive interneurons switching courtship and aggression in Drosophila (2014)
  • Author(s): Koganezawa, M., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Sex-specific functions of longitudinals lacking. (2014)
  • Author(s): Sato, K., Toba, G., Ito, H., Koganezawa, M., Binglong, Z., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Visualizing central neuron activities that mediate male courtship behavior in Drosophila. (2014)
  • Author(s): Kohatsu, S., Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] A genetic screen for Fru-target genes required for sexually-dimorphic projection patterning in Drosophila neurons. (2014)
  • Author(s): Ito, H., Sato, K., Kondo, S., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Functional characterization of the calcium channel in Drosophila embryonic muscles (2014)
  • Author(s): Hara, Y., Koganezawa, M., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] A possible involvement of non-cell- autonomous mechanism in shaping sexually dimorphic neuronal structures (2014)
  • Author(s): Kato, T., Sato, K., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Targeted mutagenesiss of the fruitless gene in Drorophia subobscura by the CRISPR/Cas9 system. (2014)
  • Author(s): Tanaka, R., Ote, M., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Neuropeptide-expressing neurons regulate sexual receptivity in Drosophila females. (2014)
  • Author(s): Miwa, Y., Koganezawa, M., Emoto, K. and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] Courtship on the treadmill: a case of Drosophila subobscura male. (2014)
  • Author(s): Higuchi, T., Kohatsu, S., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] TRF2 may act as a co-factor of fruitless, a neural masculinizing factor (2014)
  • Author(s): Chowdhury, S. Z., Sato, K., and Yamamoto, D.
  • Organizer: The 2nd Taiwan-Tohoku University Neuroscience Workshop for Young Scientists
  • Place of Presentation: The Miyagi-Zao Royal Hotel and Tohoku University, Miyagi
  • Year and Date: 2014-12-07 – 2014-12-11
• [Presentation] L-leucine添加によりオートファジー不全が改善するメカニズムとは？ (2014)
  • Author(s): 柳澤比呂子, 西藤泰昌, 河上江美子, 遠藤堅太郎, 山元大輔, 小松雅明.
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜, 神奈川県
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Searching for neurons that regulate female sexual receptivity in Drosophila (2014)
  • Author(s): Miwa, Y., Koganezawa, M., Emoto, K., and Yamamoto, D.
  • Organizer: The 37th Annual Meeting of the Molecular Biology society of Japan
  • Place of Presentation: Pacifico Yokohama, Kanagawa
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Central regulation of male courtship behavior in Drosophila melanogaster (2014)
  • Author(s): Yamamoto, D.
  • Organizer: Neural Circuits Controlling Sexual Behavior Symposium
  • Place of Presentation: Janelia Farm Research Campus, Ashburn, GA, USA.
  • Year and Date: 2014-11-09 – 2014-11-12
  • Invited
• [Presentation] Drosophila subobscuraにおけるCRISPR/Cas9システムを用いたゲノム編集. ～種特異的な求愛行動を生み出す神経基盤の探索に向けて～. (2014)
  • Author(s): 田中良弥, 大手 学, 山元 大輔.
  • Organizer: 第7回Evo-devo青年の会
  • Place of Presentation: 国立遺伝学研究所, 三島市, 静岡県
  • Year and Date: 2014-10-11 – 2014-10-12
• [Presentation] ショウジョウバエ脳神経系の雄化因子Fruitlessがもつ転写調節以外の機能 (2014)
  • Author(s): 佐藤耕世, 鳥羽岳太, 伊藤弘樹, 小金澤雅之, 山元大輔.
  • Organizer: 第6回新学術領域研究「性差構築の分子基盤」領域会議
  • Place of Presentation: 舘山寺サゴーロイヤルホテル, 浜松市, 静岡県
  • Year and Date: 2014-10-09 – 2014-10-11
• [Presentation] Molecular neuroethology of Drosophila courtship: from the gene to behavior (2014)
  • Author(s): Yamamoto, D.
  • Organizer: Seminar at Ludwig Maximilian University
  • Place of Presentation: Munich, Germany.
  • Year and Date: 2014-09-19 – 2014-09-30
  • Invited
• [Presentation] Btkはニッチで生殖細胞の増殖を停止させる (2014)
  • Author(s): Hamada-Kawaguchi, N., Nore, F. B., Smith, C. I. E., and Yamamoto, D.
  • Organizer: 日本動物学会第85回仙台大会
  • Place of Presentation: 東北大学川内北キャンパス, 仙台, 宮城県
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 生殖行動の誘発に関連するpale発現ニューロンの同定 (2014)
  • Author(s): 佐藤千晶, 山元大輔, 木村賢一.
  • Organizer: 日本動物学会第85回仙台大会
  • Place of Presentation: 東北大学川内北キャンパス, 仙台, 宮城県
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ショウジョウバエ脳神経系の雄化因子Fruitless がもつ転写調節以外の機能 (2014)
  • Author(s): 佐藤耕世，鳥羽岳太，伊藤弘樹，小金澤雅之，山元大輔.
  • Organizer: 日本動物学会第85回仙台大会
  • Place of Presentation: 東北大学川内北キャンパス, 仙台, 宮城県
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Does sex-specific neurite patterning involve cell-cell interactions in Drosophila central neurons? (2014)
  • Author(s): Kato, T., Sato, K., and Yamamoto, D.
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜, 神奈川県
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 異性に対する｢好み｣の進化をもたらす神経基盤の解明 (2014)
  • Author(s): 石川由希, 上川内あづさ, 山元大輔.
  • Organizer: 日本進化学会第16回大阪大会
  • Place of Presentation: 高槻現代劇場, 高槻市, 大阪府
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] doublesex-expressing neurons controlling female reproductive behavior in Drosophila (2014)
  • Author(s): Kimura, K., Sato, C., Koganezawa, M., and Yamamoto D.
  • Organizer: 11th International Congress of Neuroethology (ICN)
  • Place of Presentation: Sapporo, Hokkaido
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] Neural circuitry switching between courtship and aggression in Drosophila. (2014)
  • Author(s): Koganezawa, M., and Yamamoto, D.
  • Organizer: 11th International Congress of Neuroethology (ICN).
  • Place of Presentation: Sapporo, Hokkaido
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] Neural correlates of male courtship following revealed by in vivo functional imaging in Drosophila. (2014)
  • Author(s): Kohatsu, S. Yamamoto D.
  • Organizer: 11th International Congress of Neuroethology (ICN).
  • Place of Presentation: Sapporo, Hokkaido
  • Year and Date: 2014-07-28 – 2014-08-01
• [Presentation] ショウジョウバエの求愛／攻撃の切り替えに関わるfruitless発現神経回路 (2014)
  • Author(s): 小金澤雅之, 山元大輔.
  • Organizer: 平成26年度日本動物学会東北支部大会
  • Place of Presentation: 岩手大学復興祈念銀河ホール, 盛岡市, 岩手
  • Year and Date: 2014-07-12 – 2014-07-13
• [Presentation] Btk29A phosphorylates β-catenin to gate Wnt4 signaling in the niche and switches off germ cell proliferation. (2014)
  • Author(s): Hamada-Kawaguchi, N., Nore, F. B., Smith, C. I. E., Yamamoto, D.
  • Organizer: 11th Japanese Drosophila Research Conference.
  • Place of Presentation: Kanazawa, Ishikawa.Kanazawa Kagekiza, Ishikawa
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Neuronal Mechanisms Underlying Species Specificity of Mating Preference (2014)
  • Author(s): Ishikawa, Y., Kamikouchi, A., and Yamamoto, D.
  • Organizer: 11th Japanese Drosophila Research Conference
  • Place of Presentation: Kanazawa Kagekiza, Ishikawa
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Attempting targeted mutagenesis of Drosophila subobscura by the CRISPR/Cas9 system (2014)
  • Author(s): Tanaka, R. Toba, Gakuta. Yamamoto, D.
  • Organizer: 11th Japanese Drosophila Research Conference.
  • Place of Presentation: Kanazawa Kagekiza, Ishikawa
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] ショウジョウバエの性行動の遺伝解析 (2014)
  • Author(s): 山元大輔.
  • Organizer: 第22回生命科学セミナー
  • Place of Presentation: 兵庫県立大学, 神戸市, 兵庫県
  • Year and Date: 2014-04-28
  • Invited
• [Book] Epigenetic Shaping of Sociosexual Interactions (2014)
  • Author(s): Yamamoto D.
  • Total Pages: 314
  • Publisher: Elsevier
• [Book] Epigenetic Shaping of Sociosexual Interactions: An epigenetic switch of the brain sex as a basis of gendered behavior in Drosophila (2014)
  • Author(s): Sato, K. and Yamamoto, D.
  • Total Pages: 314
  • Publisher: Elsevier
• [Book] カンデル 神経科学 (2014)
  • Author(s): Kandel R. E. et al. (編), 山元大輔 (訳).
  • Total Pages: 1696
  • Publisher: メディカル・サイエンス・インターナショナル
• [Remarks] 日経バイオテク「オス同士の求愛行動は遺伝子と環境要因が関係、ショウジョウバエの実験で解明」
  • URL: https://bio.nikkeibp.co.jp/article/news/20150309/183034/
• [Remarks] 日経プレスリリース「東北大、雄同士の求愛は遺伝的素因と社会環境の影響で起きることを発見」
  • URL: http://release.nikkei.co.jp/detail.cfm?relID=381811&lindID=5
• [Remarks] 財経新聞「東北大、ハエの同性愛行動は遺伝的素因と社会環境の相互影響で起こることを発見」
  • URL: http://www.zaikei.co.jp/article/20150308/239254.html
• [Remarks] The Scientist
  • URL: http://www.the-scientist.com/?articles.view/articleNo/40410/title/Unraveling-the-Female-Fruit-Fly-Mating-Circuit/