遺伝子対遺伝子説の新展開：ペア抵抗性遺伝子群による植物と病原体の遺伝子相関

• Project Area: Correlative gene system: establishing next-generation genetics
• Project/Area Number: 26113712
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: 河野 洋治 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (00406175)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 抵抗性タンパク質 / 稲 / 耐病性 / イネ / 抵抗性遺伝子

Outline of Annual Research Achievements

植物の限られたゲノムによってどのように多様な病原体を認識し、免疫を誘導するかは十分に理解されていない。1940年代、Flor博士は、植物の病害抵抗性は、単一の優性遺伝子によって制御され、植物の抵抗性遺伝子とそれに対応する病原体の非病原性遺伝子の組み合わせによって決定される「遺伝子対遺伝子説」を提唱した。これまで申請者は、単一で機能すると考えられていた抵抗性遺伝子Pit-1と、異なる2つの抵抗性遺伝子がペアで働くペア抵抗性遺伝子RGA4とRGA5に着目して解析を行ってきた。その過程で、抵抗性タンパク質Pit-1は、5つの抵抗性タンパク質様タンパク質（Pit-2,3,4,5,6）と結合することを見出した。この結果は、1つの抵抗性タンパク質は、異なる抵抗性タンパク質と「ネットワーク」を形成していることを示唆している。これまでに、１つの抵抗性遺伝子が複数の抵抗性遺伝子とネットワークを形成する報告はなく、この知見は、植物免疫を理解する上で新しい概念となりうる。本研究では、抵抗性タンパク質ネットワークによる植物免疫機構を解明することを通じて、病原体と植物の遺伝子相関を理解する。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] University Tunku Abdul Rahman(マレーシア)
• [Int'l Joint Research] John Innes Centre(英国)
• [Int'l Joint Research] Chinese Academy of Sciences/South China Agricultural University/Institute of Plant Protection(中国)
• [Int'l Joint Research] Ohio State University(米国)
• [Journal Article] Crosstalk of Signaling Mechanisms Involved in Host Defense and Symbiosis against Microorganisms in Rice. (2016)
  • Author(s): Akamatsu, A., Shimamoto, K., and Kawano, Y.
  • Journal Title: Curr Genomic Volume: In press
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice (2015)
  • Author(s): Akamatsu, A., Uno, K., Kato, M., Wong, HL., Shimamoto, K., and Kawano, Y.
  • Journal Title: Plant Signal Behav Volume: 10 Issue: 7 Pages: e1044702-e1044702
  • DOI: 10.1080/15592324.2015.1044702
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The RhoGAP SPIN6 Associates with SPL11 and OsRac1 and Negatively Regulates Programmed Cell Death and Innate Immunity in Rice. (2015)
  • Author(s): Liu, L., Park, CH., He, F., Nagano, M., Wang, M., Bellizzi, M., Zeng, X., Liu, W., Ning, Y.,Kawano, Y., and Wang, GL.
  • Journal Title: PLoS Pathog. Volume: 11 Issue: 2 Pages: e1004629-e1004629
  • DOI: 10.1371/journal.ppat.1004629
  • Peer Reviewed
• [Journal Article] New insights into the dimerization of small GTPase Rac/ROP guanine nucleotide exchange factors in rice. (2015)
  • Author(s): Akamatsu, A., Uno, K., Kato, M., Wong, HL., Shimamoto, K., and Kawano, Y.
  • Journal Title: Plant Signal Behav. Volume: In press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Palmitoylation-dependent membrane localization of the rice R protein Pit is critical for the activation of the small GTPase OsRac1. (2015)
  • Author(s): Kawano, Y., Fujiwara, T., Yao, A., Housen, Y., Hayashi, K., and Shimamoto, K.
  • Journal Title: J Biol Chem. Volume: 289 Issue: 27 Pages: 19079-88
  • DOI: 10.1074/jbc.m114.569756
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rho family GTPase-dependent immunity in plants and animals. (2014)
  • Author(s): Kawano, Y., Kawano-Kaneko, T., and Shimamoto, K.
  • Journal Title: Front. Plant Sci. Volume: 5 Pages: 522-522
  • DOI: 10.3389/fpls.2014.00522
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance (2014)
  • Author(s): Cesari, S., Kanzaki, H., Fujiwara, T., Bernoux, M., Chalvon, V., Kawano, Y., Shimamoto, K., Dodds, P., Terauchi, R., Kroj, T.
  • Journal Title: EMBO Journal Volume: 33 Issue: 17 Pages: 1941-1959
  • DOI: 10.15252/embj.201487923
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] α-Taxilin interacts with soring nexin 4 and participates in the recycling pathway of transferrin receptor. (2014)
  • Author(s): Sakane, H., Horii, Y., Nogami, S., Kawano, Y., Kaneko-Kawano, T., and Shirataki, H.
  • Journal Title: PloS ONE Volume: 9 Issue: 4 Pages: e93509-e93509
  • DOI: 10.1371/journal.pone.0093509
  • Peer Reviewed / Open Access
• [Presentation] Elucidation of mechanisms of small GTPase OsRac1 activation by R protein Pit through OsSPIKE1 (2016)
  • Author(s): Yoji Kawano
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター（岡山県岡山市）
  • Year and Date: 2016-03-21
• [Presentation] Elucidation of mechanisms of small GTPase OsRac1 activation by R protein Pit through OsSPIKE1 (2016)
  • Author(s): Yoji Kawano
  • Organizer: 第57回 日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] R protein Pit-1 regulates disease resistance through activation of small GTPase OsRac1 by OsSPIKE1 (2015)
  • Author(s): Yoji Kawano
  • Organizer: The 4th International Conference on Biotic Plant Interaction
  • Place of Presentation: 中国（南京）
  • Year and Date: 2015-07-31
  • Int'l Joint Research
• [Presentation] Small GTPase OsRac1 is a key regulator in PAMP-triggered immunity and effector-triggered immunity (2015)
  • Author(s): Yoji Kawano
  • Organizer: 3rd Beijing International Symposium on Molecular Plant Pathology
  • Place of Presentation: 中国（北京）
  • Year and Date: 2015-07-28
  • Int'l Joint Research / Invited
• [Remarks] 研究室ホームページ
  • URL: http://sti-lab.org
• [Remarks] KAWANO Lab
  • URL: http://sti-lab.org