植物幹細胞の生殖生長相転換とエピゲノムの相互作用

• Project Area: Correlative gene system: establishing next-generation genetics
• Project/Area Number: 26113713
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Yokohama City University
• Principal Investigator: 辻 寛之 横浜市立大学, 木原生物学研究所, 准教授 (40437512)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2015: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000); Fiscal Year 2014: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: メリステム / エピゲノム / イネ / フロリゲン / 花成 / 幹細胞

Outline of Annual Research Achievements

生長相転換に伴うトランスポゾンのサイレンシングのメカニズムについて理解を深めるために、野生型イネの栄養生長メリステムと生殖生長メリステムのDNAメチローム解析を実施した。その結果、CHH配列（HはC以外の塩基）におけるシトシンのメチル化が生殖生長期においてゲノム全体で上昇することが分かった。Small RNA-seq, RNA-seq, プロテオミクスを組み合わせて解析した結果、この制御がRNAiのシステム を介していることが分かった。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Umea Plant Science Center(Sweden)
• [Int'l Joint Research] Louisiana State University(米国)
• [Journal Article] FT-like proteins induce transposon silencing in the shoot apex during floral induction in rice (2015)
  • Author(s): Shojiro Tamaki, Hiroyuki Tsuji, Ayana Matsumoto, Akiko Fujita, Zenpei Shimatani, Rie Terada, Tomoaki, Sakamoto, Tetsuya Kurata and Ko Shimamoto
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 112 Issue: 8
  • DOI: 10.1073/pnas.1417623112
  • Peer Reviewed / Open Access
• [Journal Article] Dual role of tree florigen activation complex component FD in photoperiodic growth control and adaptive response pathways. (2015)
  • Author(s): *Tylewicz, S., *Tsuji, H., *Miskoloczi, P. (*Co-first Authors), Petterle, A., Azeez, A., Jonsson, K., Shimamoto, K., Bhalerao, R.P.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 112 Issue: 10 Pages: 3140-3145
  • DOI: 10.1073/pnas.1423440112
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Hd3a promotes lateral branching in rice. (2015)
  • Author(s): Tsuji H, Tachibana C, Tamaki S, Taoka KI, Kyozuka J, Shimamoto K.
  • Journal Title: Plant J. Volume: 82 Issue: 2 Pages: 256-266
  • DOI: 10.1111/tpj.12811
  • Peer Reviewed
• [Journal Article] Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis. (2014)
  • Author(s): Ishida, T., Tabata, R., Yamada, M., Aida, M., Mitsumasu, K., Fujiwara, M., Yamaguchi, K., Shigenobu, S., Higuchi, M., Tsuji, H., Shimamoto, K., Hasebe, M., Fukuda, H., Sawa, S.
  • Journal Title: EMBO rep. Volume: 15 Issue: 11 Pages: 1202-1209
  • DOI: 10.15252/embr.201438660
  • Peer Reviewed
• [Journal Article] Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format. (2014)
  • Author(s): Fujikawa Y, Nakanishi T, Kawakami H, Yamasaki K, Sato MH, Tsuji H, Matsuoka M, Kato N.
  • Journal Title: Rice Volume: 7 Issue: 1 Pages: 11-11
  • DOI: 10.1186/s12284-014-0011-8
  • Peer Reviewed / Open Access
• [Journal Article] Florigen Signaling (2014)
  • Author(s): Tsuji H, Taoka K.
  • Journal Title: The Enzymes Volume: 35 Pages: 113-144
  • DOI: 10.1016/b978-0-12-801922-1.00005-1
  • Peer Reviewed
• [Presentation] 花成ホルモン・フロリゲンによって誘導される植物幹細胞エピゲノムのリモデリング (2015)
  • Author(s): 辻 寛之
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会年会合同大会 (BMB2015)
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-02
  • Invited
• [Presentation] Florigen-induced transposon silencing in the shoot apical meristem of rice (2015)
  • Author(s): Tsuji, H.
  • Organizer: 26th International Conference on Arabidopsis Research (ICAR2016)
  • Place of Presentation: Paris, France
  • Year and Date: 2015-07-09
  • Int'l Joint Research / Invited
• [Presentation] Florigen-induced epigenomic reprogramming in plant stem cells (2015)
  • Author(s): Tsuji, H.
  • Organizer: Establishing Next-generation Genetics
  • Place of Presentation: 奈良春日野国際フォーラム"甍"（奈良県奈良市）
  • Year and Date: 2015-05-28
  • Int'l Joint Research / Invited
• [Presentation] Coordination of flowing and gene expression by rice Hd3a florigen (2014)
  • Author(s): Tsuji, H.
  • Organizer: 12th International Conference of Rice Functional Genomics
  • Place of Presentation: Tucson, Arizona, USA
  • Year and Date: 2014-11-17
  • Int'l Joint Research / Invited
• [Presentation] he flowering hormone florigen: Its structure, receptor complex, and beyond (2014)
  • Author(s): Tsuji, H.
  • Organizer: 第38回 内藤コンファレンス
  • Place of Presentation: シャトレーゼ ガトーキングダム札幌（札幌市）
  • Year and Date: 2014-10-09
  • Int'l Joint Research / Invited
• [Remarks] Website
  • URL: http://hiroyukitsuji.tumblr.com