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運動学習における長期記憶機構の研究

Publicly Offered Research

Project AreaPrinciples of memory dynamism elucidated from a diversity of learning systems
Project/Area Number 26115504
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionSaitama University

Principal Investigator

中井 淳一  埼玉大学, 理工学研究科, 教授 (80237198)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords運動学習 / カルシウム / 記憶 / 定着 / 長期記憶 / G-CaMP / ゼブラフィッシュ
Outline of Annual Research Achievements

記憶の定着がどのように起こるのか、また、良く知られた記憶の中枢である海馬と運動学習における中枢である小脳で、記憶の定着のメカニズムの違いや共通点は何かなど、記憶の定着についてはまだよく分かっていないことが多い。運動学習の短期記憶について、小脳における運動学習では記憶が小脳皮質でおこると考えられている。しかし、記憶の定着がどのように起こるのか、そのメカニズムはまだよくわかっていない。本研究では、運動学習に関する短期記憶のメカニズムの解明を行う。
小脳学習の定着のメカニズムが解明される。記憶の理解が進むことにより、短期記憶と長期記憶の分子機構の違いが明らかになる。また、この成果は海馬などの記憶の定着のメカニズムとの相同性や、違いを知ることにつながり、脳における長期記憶の全体像の理解が深まる。老化で記憶力が落ちるメカニズムの解明やその対処法、記憶に関するヒトの疾患の原因解明、脳卒中後のリハビリの効率化や、スポーツ強化にもつながる可能性がある。
ゼブラフィッシュを用いて、optokinetic response(OKR) 用視覚刺激装置を作成し、サッケードの角度、速度を解析した。この装置を用いて、OKRの学習を行わせ、学習後のサッカードの角度、速度を解析した。エンハンサートラップラインを用いてG-CaMPを小脳顆粒細胞、下オリーブ核、Eurydendroid cellに発現させ、その際の小脳の顆粒細胞、下オリーブ核の細胞活動をG-CaMPを用いたカルシウムイメージングを用いて解析を行った。今後はプルキンエ細胞にG-CaMPを発現するトランスジェニックラインを用いたカルシウムイメージングによりさらに解析を行っていく必要がある。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (36 results)

All 2016 2015 2014 Other

All Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 12 results,  Open Access: 8 results,  Acknowledgement Compliant: 4 results) Presentation (20 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain2016

    • Author(s)
      H Monai, M Ohkura, M Tanaka, Y Oe, A Konno, H Hirai, K Mikoshiba, S Itohara, J Nakai, Y Iwai & H Hirase
    • Journal Title

      Nature communications

      Volume: 7 Issue: 1 Pages: 11100-11100

    • DOI

      10.1038/ncomms11100

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration.2015

    • Author(s)
      Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, Takamatsu N, Yoshioka K
    • Journal Title

      Cell Death Differ

      Volume: - Issue: 8 Pages: 1260-1274

    • DOI

      10.1038/cdd.2014.207

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A top-down cortical circuit for accurate sensory perception2015

    • Author(s)
      Manita S, Suzuki T, Homma C, Matsumoto T, Odagawa M, Yamada K, Ota K, Matsubara C, Inutsuka A, Sato M, Ohkura M, Yamanaka A, Yanagawa Y, Nakai J, Hayashi Y, Larkum ME, Murayama M
    • Journal Title

      Neuron

      Volume: 86(5) Issue: 5 Pages: 1304-16

    • DOI

      10.1016/j.neuron.2015.05.006

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element2015

    • Author(s)
      Masaaki Sato, Masako Kawano, Masamichi Ohkura, Keiko Gengyo-Ando, Junichi Nakai and Yasunori Hayashi
    • Journal Title

      PLoS ONE

      Volume: 未定 Issue: 5 Pages: 1-13

    • DOI

      10.1371/journal.pone.0125354

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of genetically encoded calcium indicators for monitoring action potentials in mammalian brain by two-photon excitation fluorescence microscopy.2015

    • Author(s)
      Podor B, Hu Y, Ohkura M, Nakai J, Croll R, Fine A
    • Journal Title

      Neurophotonics

      Volume: 2(2):021014 Issue: 2 Pages: 1-7

    • DOI

      10.1117/1.nph.2.2.021014

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RING finger protein 121 facilitates the degradation and membrane localization of voltage-gated sodium channels2015

    • Author(s)
      Ogino, K., Low, S.E., Yamada, K., Saint-Amant, L., Zhou, W., Muto, A., Asakawa, K., Nakai, J., Kawakami, K., Kuwada, J.Y., and Hirata, H.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 112 Issue: 9 Pages: 2859-2864

    • DOI

      10.1073/pnas.1414002112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rational design of a high-affinity, fast, red calcium indicator R-CaMP22015

    • Author(s)
      Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
    • Journal Title

      Nat Methods

      Volume: 12 Issue: 1 Pages: 64

    • DOI

      10.1038/nmeth.3185

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mitochondrial Ca2+ uptake by the voltage-dependent anion channel 2 regulates cardiac rhythmicity.2014

    • Author(s)
      Shimizu H, Schredelseker J, Huang J, Lu K, Naghdi S, Lu F, Franklin S, Fiji HD, Wang K, Zhu H, Tian C, Lin B, Nakano H, Ehrlich A, Nakai J, Stieg AZ, Gimzewski JK, Nakano A, Goldhaber JI, Vondriska TM, Hajnoczky G, Kwon O, Chen JN.
    • Journal Title

      Elife

      Volume: 4 Pages: 1-20

    • DOI

      10.7554/elife.04801

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reward-timing-dependent bidirectional modulation of cortical microcircuits during optical single-neuron operant conditioning.2014

    • Author(s)
      Hira R et al.
    • Journal Title

      Nature Communications

      Volume: 5 Issue: 1 Pages: 5551-5551

    • DOI

      10.1038/ncomms6551

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Excitation propagation in three-dimensional engineered hearts using decellularized extracellular matrix.2014

    • Author(s)
      Yasui H, Lee JK, Yoshida A, Yokoyama T, Nakanishi H, Miwa K, Naito AT, Oka T, Akazawa H, Nakai J, Miyagawa S, Sawa Y, Sakata Y, Komuro I
    • Journal Title

      Biomaterials

      Volume: 35 Issue: 27 Pages: 7839-7850

    • DOI

      10.1016/j.biomaterials.2014.05.080

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Toll-like receptor 9 protects non-immune cells from stress by modulating mitochondrial ATP synthesis through the inhibition of SERCA2.2014

    • Author(s)
      Shintani Y, Drexler HC, Kioka H, Terracciano CM, Coppen SR, Imamura H, Akao M, Nakai J, Wheeler AP, Higo S, Nakayama H, Takashima S, Yashiro K, Suzuki K.
    • Journal Title

      EMBO Reports

      Volume: 15 Issue: 4 Pages: 438-445

    • DOI

      10.1002/embr.201337945

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Large-scale imaging of subcellular calcium dynamics of cortical neurons with G-CaMP6-actin2014

    • Author(s)
      小林千晃, 他
    • Journal Title

      Neuroreport

      Volume: 25(7) Issue: 7 Pages: 501-506

    • DOI

      10.1097/wnr.0000000000000126

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 意識下活動動物からの特定神経活動記録法の開発2016

    • Author(s)
      山下哲, 犬束歩, Srikanta Chowdhury, 中井淳一, 大倉正道, 田口徹, 桑木共之, 山中章弘
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光刺激・計測によるマウス大脳皮質層間信号統合のin vivo解析2016

    • Author(s)
      小泉協, 佐藤正晃, 大倉正道, 中井淳一, 林康紀, 八尾寛
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 2光子イメージング法での神経発火活動の検出に適した蛍光カルシウムプローブ蛋白質の比較検討2016

    • Author(s)
      大倉正道, Borbala Podor, Yi-ling Hu, Roger Croll, Alan Fine, 中井淳一
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] G-CaMP7発現マウス大脳皮質脳細胞活動の細胞-領野スケール長期イメージング実験解析法2015

    • Author(s)
      茂木優貴, 安藤恵子, 沖篤志, 岩井陽一, 毛内拡, 平瀬肇, 大倉正道, 中井淳一
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Real-time visualization of neuronal activity in zebrafish and C. elegans.2015

    • Author(s)
      Nakai J, Ohkura M, Kagawa-Nagamura Y, Muto A, Inoue M, Bito H, Kawakami K, Gengyo-Ando K
    • Organizer
      第38回日本神経科学大会シンポジウム
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A new transgenic mouse model revealed a role of astrocytic calcium in vivo2015

    • Author(s)
      Tanaka M, Shih P-Y, Gomi H, Yoshida T, Nakai J, Ando R, Furuichi T, Mikoshiba K, Samyanov A, Itohara S.
    • Organizer
      第88回日本薬理学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rational design of a high-affinity, fast, red calcium indicator R-CaMP2.2015

    • Author(s)
      井上昌俊、竹内敦也、堀金慎一郎、大倉正道、安藤恵子、藤井哉、上條諭志、竹本―木村さやか、狩野方伸、中井淳一、喜多村和郎、尾藤晴彦
    • Organizer
      第88回日本薬理学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 個体(線虫) レベルでの超多並列刺激応答モニターシステム2014

    • Author(s)
      藤泰一、高橋尚也、安藤恵子、大倉正道、中井淳一、西垣功一
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 低分子量GTPase RAB-11の新規結合因子REI-1/REI-2は受精後のRAB-11再局在化を制御する2014

    • Author(s)
      坂口愛沙、佐藤美由紀、安藤恵子、佐藤克哉、中井淳一、佐藤健
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Imaging Deep-Layer Neuronal Activity in Mouse Barrel Cortex using 1040-nm Excitation of a Red Fluorescent Protein Calcium Indicator2014

    • Author(s)
      Carta S, Liu W, Sych Y, Voigt FF, Chen JL, Schneider B, Ohkura M, Nakai J, Helmchen F
    • Organizer
      Neuroscience 2014
    • Place of Presentation
      Washington, DC USA
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] A comparative study of the two-photon performances of GCaMPs and GECOs2014

    • Author(s)
      Podor B, Zhao Y, Wu J, Hu Y-L, Ohkura M, Nakai J, Campbell R, Croll R, Fine A
    • Organizer
      Neuroscience 2014
    • Place of Presentation
      Washington, DC USA
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Neuronal mechanisms of decision making in C. elegans olfactory behavior revealed by a highly integrated microscope system.2014

    • Author(s)
      Tanimoto Y, Yamazoe A, Fujita K, Kawazoe Y, Miyanishi Y, Yamazaki SJ, Gengyo-Ando K, Nakai J, Fei X, Iwasaki Y, Hashimoto K, Kimura K
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-09 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Single-neuron operant conditioning by two-photon imaging induces reward-timing-dependent modulation in cortical microcircuit.2014

    • Author(s)
      Hira R, Ohkubo F, Mazamizu Y, Ohkura M, Nakai J, Okada T, Matsuzaki M
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-09 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] The top-down circuit for sensory perception in the cerebral cortex of the mouse.2014

    • Author(s)
      Manita S, Suzuki T, Homma C, Matsumoto T, Odagawa M, Yamada K, Ota K, Matsubara C, Inutsuka A, Sato M, Ohkura M, Yamanaka A, Yanagawa Y, Nakai J, Hayashi Y, Larkum ME, Murayama M
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-09 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Imaging of the neural circuit activities during retrieval of behavioral program for active avoidance in zebrafish.2014

    • Author(s)
      Kakinuma H, Aoki R, Aoki T, Yamazaki M, Shiraki T, Takahoko M, Eizumi K, Koide T, Yoshihara Y, Nakai J, Kawakami K, Okamoto H
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-09 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Visualization of cerebral cortical calcium dynamics by a transgenic mouse that express G-CaMP7 in neurons and glia.2014

    • Author(s)
      Monai H, Ohkura M, Tanaka M, Itohara S, Nakai J, Hirase H
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-09 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] JSAP1 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration.2014

    • Author(s)
      Sato T, Ohkura M, Nakai J, Takamatsu N, Yoshioka K
    • Organizer
      第34回日本神経科学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-09 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 蛍光カルシウムプローブ G-CaMP による in vivo カルシウムイメージング2014

    • Author(s)
      中井淳一、大倉正道, 安藤恵子
    • Organizer
      第58回日本薬学会関東支部大会
    • Place of Presentation
      昭和大学
    • Year and Date
      2014-10-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] ホヤ幼生の遊泳運動神経回路の構造と生理機能の解析2014

    • Author(s)
      堀江健生, 大倉正道, 日下部岳広, 中井淳一, 中川将司
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞触覚センサのための小型蛍光観察システムの開発2014

    • Author(s)
      民山浩輔, 清水正宏, 宮坂恒太, 小椋利彦, 中井淳一, 大倉正道, 細田耕
    • Organizer
      第32回日本ロボット学会学術講演会
    • Place of Presentation
      九州産業大学
    • Year and Date
      2014-09-04 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Book] Optogenetic manipulation and probing. 『Optogenetics: light-sensing proteins and their applications』Part 2, Chapter 92015

    • Author(s)
      Ohkura M, Sadakari J, Nakai J
    • Total Pages
      409
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Book] Optical Imaging of Neocortical Dynamics, Neuromethods, vol. 852014

    • Author(s)
      Takata N., Shinohara Y., Ohkura M., Mishima T., Nakai J. & Hirase H.
    • Total Pages
      296
    • Publisher
      Springer Science Business Media
    • Related Report
      2014 Annual Research Report
  • [Remarks] 埼玉大学脳末梢科学研究センター

    • URL

      http://subsi.saitama-u.ac.jp/

    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] カルシウム指示遺伝子2014

    • Inventor(s)
      尾藤晴彦, 井上 昌俊, 竹内敦也, 中井淳一, 大倉 正道
    • Industrial Property Rights Holder
      尾藤晴彦, 井上 昌俊, 竹内敦也, 中井淳一, 大倉 正道
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-06-11
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-28  

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