社会認知を介した意思決定に関わる神経ネットワークの解明

• Project Area: Principles of memory dynamism elucidated from a diversity of learning systems
• Project/Area Number: 26115508
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Okayama University (2015); The University of Tokyo (2014)
• Principal Investigator: 竹内 秀明 岡山大学, 自然科学研究科, 准教授 (00376534)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 神経科学 / 細胞系譜 / 最初期遺伝子 / 社会性行動 / 脳

Outline of Annual Research Achievements

＜最初期遺伝子を検索・同定＞本年度はメダカの個体記憶を介した配偶者選択に関わる神経ネットーワークを包括的に同定する目的で、メダカ脳で最初期遺伝子を検索・同定した。方法はPTZ（GABA阻害剤）添加によって誘導される癲癇依存に発現が誘導される遺伝子群をマイクロアレイによって検索した。約15,000遺伝子について発現量を比較したところ、18種類の遺伝子が有為に発現上昇することを定量的RT-PCRで確認した。そのうち２つの遺伝子を用いて脳活動のマッピングを行ったところ、メダカメスがオスと長時間見ていた時に活性化している脳領域（終脳背側の一部）を同定した。
＜脳領域特異的な条件的遺伝子操作法の確立＞これまでに、メダカの条件的遺伝子操作法を確立し、成体脳において細胞系譜単位（単一の神経幹細胞から発生したニューロン群）を遺伝学的にラベルすることに成功している。さらに一部の細胞系譜単位は終脳背側ではブロック化していることを見出している（PLoS One 8:e66597,2013）。本年度は終脳背側の一つの細胞系譜単位が(1)で同定した活性化脳領域に対応することを見出した。
＜個体記憶を介した行動を定量化する新規行動実験系の確立＞本年度は新規な行動実験系を２つ確立し、以下の２点を示した。(1) メダカの三角関係（オス、オス、メス）において、オスはライバルオスに配偶者防衛行動を示すことで、メスがライバルオスを記憶することを妨害している（Frontier in Zoology in press） 。メダカメスはそばにいたオスを記憶して、配偶者として選択する傾向があるので、メスがライバルオスを選択することを妨害していると考えられる。(2) メスは排卵前には視覚記憶したオスに選択的に近づき行動を示すが、排卵後はオスに対する選択性がなくなる（Zoological Science in press）。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Emory University(米国)
• [Journal Article] Ontogeny and sexual differences in swimming proximity to conspecifics in response to visual cues in medaka fish (2016)
  • Author(s): Y. Isoe, Y. Konagaya, S. okoi, T.Kubo, H. Takeuchi
  • Journal Title: Zoological Science Volume: in press
  • NAID: 210000176073
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mate-guarding behavior enhances male reproductive success via familiarization with mating partners in medaka fish (2016)
  • Author(s): Saori Yokoi, Ansai Satoshi, Masato Kinoshita, Kiyoshi Naruse, LarryJ Young, Teruhiro Okuyama and Hideaki Takeuchi
  • Journal Title: Frontiers in Zoology Volume: in press
  • NAID: 120006331985
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An Essential Role of the Arginine Vasotocin System in Mate-Guarding Behaviors in Triadic Relationships of Medaka Fish (Oryzias latipes). (2015)
  • Author(s): Yokoi S, Okuyama T, Kamei Y, Naruse K, Taniguchi Y, Ansai S, Kinoshita M, Young LJ, Takemori N, Kubo T, Takeuchi H
  • Journal Title: PLoS Genetics Volume: なし Issue: 2 Pages: e1005009-e1005009
  • DOI: 10.1371/journal.pgen.1005009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic control of startle behavior in medaka fish (2014)
  • Author(s): Tsuboko S, Kimura T, Shinya M, Suehiro Y, Okuyama T, Shimada A, Takeda H, Naruse K, Kubo T, Takeuchi H
  • Journal Title: PLoS one Volume: 9 Issue: 11 Pages: e112527-e112527
  • DOI: 10.1371/journal.pone.0112527
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Exploring the neural geography of the social brain using medaka fish (2016)
  • Author(s): Hideaki Takeuchi (Keynote lecture)
  • Organizer: 17th Australia & New Zealand Zebrafish Meeting
  • Place of Presentation: Flinders, Australia
  • Year and Date: 2016-02-03
  • Int'l Joint Research / Invited
• [Presentation] Exploring the neural geography of the social brain using medaka fish (2016)
  • Author(s): Hideaki Takeuchi (Keynote lecture)
  • Organizer: 3rd Medaka Strategic Meeting
  • Place of Presentation: Flinders, Australia
  • Year and Date: 2016-02-01
  • Int'l Joint Research / Invited
• [Presentation] Analysis of mechanism underlying brain growth accompanied by neurogenesis using medaka fish (Oryzias latipes) (2014)
  • Author(s): Yasuko Isoe, Teruhiro Okuyama, Masahito Hoki, Yuji Suehiro, Genki Yamagishi,Kiyoshi Naruse, Masato Kinoshita, Yasuhiro Kamei, Shigenori Nonaka Atushi Shimizu, Takeo Kubo, Hideaki Takeuchi
  • Organizer: Neuroscience 2014/ SfN(北米神経学会)
  • Place of Presentation: アメリカ・ワシントンD.C.
  • Year and Date: 2014-11-17
• [Presentation] Analysis of mechanism underlying brain growth accompanied by neurogenesis using medaka fish (Oryzias latipes) (2014)
  • Author(s): Yasuko Isoe, Teruhiro Okuyama, Masahito Hoki, Yuji Suehiro, Genki Yamagishi,Kiyoshi Naruse, Masato Kinoshita, Yasuhiro Kamei, Shigenori Nonaka Atushi Shimizu, Takeo Kubo, Hideaki Takeuchi
  • Organizer: Neuro2014 / The 36th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋
  • Year and Date: 2014-09-09
• [Presentation] Analysis of mechanism underlying brain growth accompanied by neurogenesis using medaka fish (Oryzias latipes) (2014)
  • Author(s): Yasuko Isoe, Teruhiro Okuyama, Masahito Hoki, Yuji Suehiro, Genki Yamagishi,Kiyoshi Naruse, Masato Kinoshita, Yasuhiro Kamei, Shigenori Nonaka Atushi Shimizu, Takeo Kubo, Hideaki Takeuchi
  • Organizer: 2014 International Congress of Neuroethology/The Japanese Society for comparative Physiology and Biochemistry
  • Place of Presentation: 札幌
  • Year and Date: 2014-07-29
• [Presentation] Searching for genes affecting visually-evoked startle response properties with inbred strains of Medaka (Oryzias latipes) -- Genetically Affected Escape Modes in Solitary and Collective States of Medaka Fish (2014)
  • Author(s): Tsuboko S, Kimura T, Shinya M, Suehiro Y, Okuyama T, Shimada A, Takeda H, Naruse K, Kubo T, Takeuchi H
  • Organizer: 2014 International Congress of Neuroethology/The Japanese Society for comparative Physiology and Biochemistry
  • Place of Presentation: 札幌
  • Year and Date: 2014-07-28
• [Presentation] Analysis of mechanism underlying brain growth accompanied by neurogenesis using medaka fish (Oryzias latipes) (2014)
  • Author(s): Yasuko Isoe, Teruhiro Okuyama, Masahito Hoki, Yuji Suehiro, Genki Yamagishi,Kiyoshi Naruse, Masato Kinoshita, Yasuhiro Kamei, Shigenori Nonaka Atushi Shimizu, Takeo Kubo, Hideaki Takeuchi
  • Organizer: 2nd Strategic Meeting for Medaka Research
  • Place of Presentation: セルビア、スペイン
  • Year and Date: 2014-04-10 – 2014-04-12
• [Presentation] Searching for genes affecting visually-evoked startle response properties with medaka inbred strains (2014)
  • Author(s): Tsuboko S, Kimura T, Shinya M, Suehiro Y, Okuyama T, Shimada A, Takeda H, Naruse K, Kubo T, Takeuchi H
  • Organizer: 2nd Strategic Meeting for Medaka Research
  • Place of Presentation: セルビア、スペイン
  • Year and Date: 2014-04-10 – 2014-04-12
• [Presentation] Behavioral polymorphism in medaka strains: genetic factor matters? (2014)
  • Author(s): Mu-Yun Wang, Takeo Kubo and Hideaki Takeuchi
  • Organizer: 2nd Strategic Meeting for Medaka Research
  • Place of Presentation: セルビア、スペイン
  • Year and Date: 2014-04-10 – 2014-04-12
• [Presentation] Proximate and ultimate causes of socially-regulated mating preference in medaka fish (2014)
  • Author(s): Hideaki Takeuchi
  • Organizer: 2nd Strategic Meeting for Medaka Research
  • Place of Presentation: セルビア、スペイン
  • Year and Date: 2014-04-10
  • Invited
• [Book] 動物行動の分子生物学 (2014)
  • Author(s): 竹内秀明
  • Total Pages: 27
  • Publisher: 裳華房
• [Remarks] Researchmap
  • URL: http://researchmap.jp/takeuchi/?lang=japanese
• [Remarks] 恋敵からメスを守るオスメダカ
  • URL: https://www.s.u-tokyo.ac.jp/ja/press/4154/