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Cellular environment-dependent RNA methylation at synapse during learning and memory

Publicly Offered Research

Project AreaPrinciples of memory dynamism elucidated from a diversity of learning systems
Project/Area Number 26115515
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyoto University

Principal Investigator

王 丹  京都大学, 物質-細胞統合システム拠点, 助教 (50615482)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
KeywordsRNA修飾 / メチル化 / シナプス / 学習 / 記憶 / RNA methylation / forebrain / mouse / immuno-capture / massive / parallel sequencing
Outline of Annual Research Achievements

The goal of this project is to understand the function of N6-methyladenosine (m6A), a highly prevalent mRNA modification in the brain. m6A has been reported to increases along aging and regulates expression level of many proteins important for neuronal signaling, including dopamine receptors. Variations in the m6A modification enzymes not only predispose to obesity in humans, but also are associated with aging-dependent reduced brain volume, loss of memory, and risk of alcohol dependence. We hypothesized that m6A modulates spatiotemporal regulation of neuronal gene expression at synapse, which in turn, regulate cellular response of neurons to learning-related stimuli to store information of past events. This cellular mechanism may play critically important roles in aging-, nutrition-, alcohol-dependent memory dynamism. Within two years, we have successfully devised a sequencing methods using low-input amount of RNA materials and generated a list of genes that are localized to neuronal synapses by performing immuno-capture of m6A followed by deep sequencing. The data are directing to a synaptic epitranscriptomics scheme that may regulate local synaptic function by regulating local RNA dynamics. Such dynamic regulation is highly likely to play a critical role in rapid response to various stimuli that neurons receive such as learning-related activities. Currently the results are being analyzed for publication in high-impact journals.

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (9 results)

All 2015 2014

All Presentation (9 results) (of which Int'l Joint Research: 5 results)

  • [Presentation] A m6A epitranscriptome at mouse synapse2015

    • Author(s)
      王丹
    • Organizer
      International Symposium for RIKEN Epigenetics Program 2016
    • Place of Presentation
      理化学研究所 和光市
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dendritic RNA modification in hippocampal neurons2015

    • Author(s)
      小原孝之
    • Organizer
      多様性から明らかにする記憶ダイナミズムの共通原理
    • Place of Presentation
      京都ガーデンパレス(京都府・京都市)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ECHO-liveFISH: towards "lightning up" gene expression upon learning and memory2015

    • Author(s)
      大本育実
    • Organizer
      多様性から明らかにする記憶ダイナミズムの共通原理
    • Place of Presentation
      京都ガーデンパレス(京都府・京都市)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An m6A epitranscriptome at adult mouse synapse2015

    • Author(s)
      Liao Youqi
    • Organizer
      多様性から明らかにする記憶ダイナミズムの共通原理
    • Place of Presentation
      京都ガーデンパレス(京都府・京都市)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Synaptic m6A modification in adult mouse brain2015

    • Author(s)
      王丹
    • Organizer
      多様性から明らかにする記憶ダイナミズムの共通原理
    • Place of Presentation
      京都ガーデンパレス(京都府・京都市)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経細胞におけるRNAm6A修飾の役割2015

    • Author(s)
      小原孝之
    • Organizer
      日本RNA学会
    • Place of Presentation
      札幌
    • Year and Date
      2015-07-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] マウス成体脳シナプスにおけるm6A-RNAのトランスクリプトーム解析2015

    • Author(s)
      Liao Youqi
    • Organizer
      第17回日本RNA学会
    • Place of Presentation
      札幌
    • Year and Date
      2015-07-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] The Role of m6A Modification in Neuron Functions2015

    • Author(s)
      小原孝之
    • Organizer
      第14回京都大学・国立台湾大学・筑波大学合同「分子細胞生物学シンポジウム」
    • Place of Presentation
      京都大学
    • Year and Date
      2015-06-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cellular environment-dependent RNA methylation at synapse during learning and memory2014

    • Author(s)
      王 丹
    • Organizer
      多様性を明らかにする記憶ダイナミズムの共通原理
    • Place of Presentation
      札幌
    • Year and Date
      2014-06-16 – 2014-06-17
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-28  

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