細菌の走性における数的多様性の解明

• Project Area: Spying minority in biological phenomena -Toward bridging dynamics between individual and ensemble processes-
• Project/Area Number: 26115706
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: 井上 圭一 名古屋工業大学, 工学(系)研究科(研究院), 助教 (90467001)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000); Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: ロドプシン / Cheタンパク質 / 一分子測定 / 全反射蛍光顕微鏡

Outline of Annual Research Achievements

本研究では細菌の走光性に関わる光受容体である、センサリーロドプシン（SR）－トランスデューサータンパク質(Htr)複合体と、Cheタンパク質の間の信号伝達過程を一分子観察し、これらのタンパク質間の相互作用を通じた、光信号の増幅過程を定量的に明らかにすることを目的とする。そして昨年度および今年度において、新規に構築した三波長レーザー一分子蛍光観察顕微鏡の構築を行うことに成功した。それにより従来よりもかなり明瞭な形で多数の一分子像が得ることができるようになり、Cy3やCy5などの新たな蛍光色素を用いた観察についても当初の予定通り達成することができた。
一方でこれまで困難だったHtrIIタンパク質についても、今年度その精製方法について検討を行った。その結果タンパク質の溶出時に用いるイミダゾール濃度をコンピューター制御によって連続的にスキャンすることで、ほぼ他の内在性タンパク質が混入しない形で精製することに成功した。またCys変異を導入した変異体については蛍光色素によるラベリングにも成功している。
これらの成果により本研究期間において、多波長レーザー一分子顕微鏡、SR及びHtrの精製法、蛍光ラベリング法など主要な技術的要件を全て確立することができた。これにより多様な吸収波長を持つ様々なSRおよびHtrについて、一分子測定によるタンパク質間信号伝達時の構造変化をsub-nmスケールで観察することが可能になり、また光活性化時に相互作用するCheタンパク質を直接計数することによって、信号増幅の度合いを精密に研究することができると期待される。今後は本研究によって確立された技術を用いて、これらの課題を明らかにし、論文として公表することを目指す。
また本研究において関連する多くのロドプシン分子について研究を行う中で様々な知見を得、12報の論文と12件の招待講演としてその成果を報告した。これらについても本研究の成果として合わせて報告する。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Asymmetric functional conversion of eubacterial light-driven ion pumps (2016)
  • Author(s): K. Inoue, Y. Nomura, H. Kandori*
  • Journal Title: J. Biol. Chem. Volume: in press Issue: 19 Pages: 9883-9893
  • DOI: 10.1074/jbc.m116.716498
  • Peer Reviewed / Open Access
• [Journal Article] Mutant of a light-driven sodium ion pump can transport cesium ions (2016)
  • Author(s): M. Konno, Y. Kato, H. E. Kato, K. Inoue, O. Nureki, H. Kandori*
  • Journal Title: J. Phys. Chem. Lett. Volume: 7 Issue: 1 Pages: 51-55
  • DOI: 10.1021/acs.jpclett.5b02385
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] イオンをポンプするロドプシンの作動機構 (2016)
  • Author(s): 井上圭一
  • Journal Title: 日本レーザー医学会誌 Volume: 36 Pages: 466-472
  • NAID: 130005966838
• [Journal Article] Kinetic analysis of H+-Na+ selectivity in a light-driven Na+-pumping rhodopsin (2015)
  • Author(s): Y. Kato , K. Inoue, H. Kandori*
  • Journal Title: J. Phys. Chem. Lett. Volume: 6 Issue: 24 Pages: 5111-5115
  • DOI: 10.1021/acs.jpclett.5b02371
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A new group of eubacterial light-driven retinal-binding proton pumps with an unusual cytoplasmic proton donor (2015)
  • Author(s): A. Harris, M. Ljumovic, A. N. Bondar, Y. Shibata, S. Ito, K. Inoue, H. Kandori, L. S. Brown*
  • Journal Title: Biochim. Biophys. Acta Volume: 1847 Issue: 12 Pages: 1518-1529
  • DOI: 10.1016/j.bbabio.2015.08.003
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ultrafast photoreaction dynamics of a light-driven sodium-ion-pumping retinal protein from Krokinobacter eikastus revealed by femtosecond time-resolved absorption spectroscopy (2015)
  • Author(s): S. Tahara, S. Takeuchi, R. Abe-Yoshizumi, K. Inoue, H. Ohtani, H. Kandori*, T. Tahara*
  • Journal Title: J. Phys. Chem. Lett. Volume: 6 Issue: 22 Pages: 4481-4486
  • DOI: 10.1021/acs.jpclett.5b01994
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The role of the NDQ motif in sodium-pumping rhodopsins (2015)
  • Author(s): K. Inoue , M. Konno, R. Abe-Yoshizumi, H. Kandori*
  • Journal Title: Angew. Chem. Int. Ed. Volume: 54 Issue: 39 Pages: 11536-11539
  • DOI: 10.1002/anie.201504549
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural basis for Na+ transport mechanism by a light-driven Na+ pump (2015)
  • Author(s): H. E. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Kato, H. Ono, M. Konno, S. Hososhima, T. Ishizuka, M. R. Hoque, H. Kunitomo, J. Ito, S. Yoshizawa, K. Yamashita, M. Takemoto, T. Nishizawa, R. Taniguchi, K. Kogure, A. D. Maturana, Y. Iino, H. Yawo, R. Ishitani, H. Kandori*, O. Nureki*
  • Journal Title: Nature Volume: 未定 Issue: 7550 Pages: 48-53
  • DOI: 10.1038/nature14322
  • Peer Reviewed / Open Access
• [Journal Article] Light-driven ion-translocating rhodopsins in marine bacteria (2015)
  • Author(s): Keiichi Inoue, Yoshitaka Kato, Hideki Kandori
  • Journal Title: Trends in Microbiology Volume: 23 Issue: 2 Pages: 91-98
  • DOI: 10.1016/j.tim.2014.10.009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] なぜ光で細胞を制御できるのか？ロドプシンの動作原理 (2015)
  • Author(s): 井上圭一
  • Journal Title: 実験医学 Volume: 33 Pages: 3048-3053
• [Journal Article] Structural Basis for Na+ Transport Mechanism by a Light-Driven Na+ Pump (2015)
  • Author(s): H. E. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Kato, H. Ono, M. Konno, T. Ishizuka, M. R. Hoque, S. Hososhima, H. Kunitomo, J. Ito, S. Yoshizawa, K. Yamashita, M. Takemoto, T. Nishizawa, R. Taniguchi, K. Kogure, A. D. Maturana, Y. Iino, H. Yawo, R. Ishitani, H. Kandori, O. Nureki
  • Journal Title: Nature Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Converting a light-driven proton pump into a light-gated proton channel (2015)
  • Author(s): K. Inoue, T. Tsukamoto, K. Shimono, Y. Suzuki, S. Miyauchi, S. Hayashi, H. Kandori, Y. Sudo*
  • Journal Title: J. Am. Chem. Soc. Volume: 137 Issue: 9 Pages: 3291-3299
  • DOI: 10.1021/ja511788f
  • Peer Reviewed
• [Journal Article] Na+ transport by sodium ion pump rhodopsin is resistant to environmental change - a comparison of photocycles of Na+ and Li+ transport processes (2015)
  • Author(s): K. Inoue, H. Ono, H. Kandori*
  • Journal Title: Chem. Lett. Volume: 44 Issue: 3 Pages: 294-296
  • DOI: 10.1246/cl.141023
  • NAID: 130004868387
  • Peer Reviewed
• [Journal Article] Spectroscopic study of a light-driven chloride ion pump from marine bacteria (2014)
  • Author(s): Keiichi Inoue, Faisal Hammad Mekky Koua, Yoshitaka Kato, Rei Abe-Yoshizumi and Hideki Kandori
  • Journal Title: J. Phys. Chem. B Volume: 118 Issue: 38 Pages: 11190-11199
  • DOI: 10.1021/jp507219q
  • Peer Reviewed / Open Access
• [Journal Article] FTIR spectroscopy of a light-driven compatible sodium ion-proton pumping rhodopsin at 77 K (2014)
  • Author(s): Ono Hikaru, Keiichi Inoue, Rei Abe-Yoshizumi and Hideki Kandori
  • Journal Title: J. Phys. Chem. B, Volume: 118 Issue: 18 Pages: 4784-4792
  • DOI: 10.1021/jp500756f
  • Peer Reviewed / Open Access
• [Journal Article] Molecular and evolutionary aspects of microbial sensory rhodopsins (2014)
  • Author(s): Keiichi Inoue, Takashi Tsukamoto, Yuki Sudo
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1837 Issue: 5 Pages: 562-577
  • DOI: 10.1016/j.bbabio.2013.05.005
  • Peer Reviewed
• [Journal Article] 微生物型ロドプシンの信号伝達の解明に向けた1分子観察法の開発 (2014)
  • Author(s): 井上圭一
  • Journal Title: 日本生化学会誌 生化学・近畿支部特集「生化学に新たな視点を与える技術の開発とその応用」 Volume: 86 Pages: 160-166
• [Journal Article] 光駆動ナトリウムポンプの発見 (2014)
  • Author(s): 井上圭一, 神取秀樹
  • Journal Title: 生物物理 Volume: 54 Pages: 106-107
  • NAID: 130003391410
• [Presentation] 光受容膜タンパク質ロドプシンの光化学の基礎研究と応用 (2016)
  • Author(s): 井上圭一
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京田辺
  • Year and Date: 2016-03-24
  • Invited
• [Presentation] 光駆動Na+ポンプのカチオン結合箇所に見られる特異な水素結合構造 (2016)
  • Author(s): 伊藤奨太、杉田真也、吉住玲、井上圭一、岩田達也、岩城雅代、神取秀樹
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京田辺
  • Year and Date: 2016-03-24
• [Presentation] フェムト~ピコ秒領域におけるナトリウムイオンポンプロドプシンKR2の光反応ダイナミクスのpH依存性とその起源 (2016)
  • Author(s): 田原進也、竹内佐年、吉住玲、井上圭一、大谷弘之、神取秀樹、田原太平
  • Organizer: 日本化学会第96春季年会
  • Place of Presentation: 京田辺
  • Year and Date: 2016-03-24
• [Presentation] Light-driven sodium pump rhodopsin and its transport mechanism (2016)
  • Author(s): K. Inoue
  • Organizer: OWLS (Optics Within Life Sciences)
  • Place of Presentation: Munbai, India
  • Year and Date: 2016-03-16
  • Int'l Joint Research / Invited
• [Presentation] 細菌の走性における数的多様性の解明 (2016)
  • Author(s): 井上圭一
  • Organizer: 新学術領域「少数性生物学」研究成果報告会
  • Place of Presentation: 東京
  • Year and Date: 2016-03-15
• [Presentation] 光駆動ナトリウムポンプのH+‐Na+選択性の過渡吸収測定による解析 (2016)
  • Author(s): 加藤善隆、井上圭一、神取秀樹
  • Organizer: 日本生物物理学会中部支部講演会
  • Place of Presentation: 岡崎
  • Year and Date: 2016-02-29
• [Presentation] 真正細菌のクロライドポンプ、プロトンポンプの機能転換および光反応の解析 (2016)
  • Author(s): 野村祐梨香、井上圭一、神取秀樹
  • Organizer: 日本生物物理学会中部支部講演会
  • Place of Presentation: 岡崎
  • Year and Date: 2016-02-29
• [Presentation] Engineering potassium and cesium pumping rhodopsin from KR2 (2016)
  • Author(s): M. Konno, Y. Kato, H. E. Kato, K. Inoue, O. Nureki, H. Kandori
  • Organizer: Gordon Research Conference on "Photosensory Receptors & Signal Transduction”
  • Place of Presentation: Galveston, U.S.A.
  • Year and Date: 2016-01-24
  • Int'l Joint Research
• [Presentation] Specific hydrogen bonding network of Na+ binding site in light-driven Na+ pumping rhodopsin (2016)
  • Author(s): S. Ito, S. Sugita, R. Abe-Yoshizumi, T. Iwata, K. Inoue, M. Iwaki, H. Kandori
  • Organizer: Gordon Research Conference on "Photosensory Receptors & Signal Transduction”
  • Place of Presentation: Galveston, U.S.A.
  • Year and Date: 2016-01-24
  • Int'l Joint Research
• [Presentation] Optical activation of G-protein by microbial rhodopsin chimera (2016)
  • Author(s): K. Yoshida, T. Yamashita, R. Abe-Yoshizumi, M. Tanaka, K. Inoue, K. Sasaki, Y. Shichida, H. Kandori
  • Organizer: Gordon Research Conference on "Photosensory Receptors & Signal Transduction”
  • Place of Presentation: Galveston, U.S.A.
  • Year and Date: 2016-01-24
  • Int'l Joint Research
• [Presentation] Engineering potassium and cesium pumping rhodopsin from KR2 (2016)
  • Author(s): M. Konno, Y. Kato, H. E. Kato, K. Inoue, O. Nureki, H. Kandori
  • Organizer: Gordon Research Seminar on "Photosensory Receptors & Signal Transduction"
  • Place of Presentation: Galveston, U.S.A.
  • Year and Date: 2016-01-23
  • Int'l Joint Research
• [Presentation] ATR-FTIR Spectroscopy Reveal the Specific Hydrogen Bonding Network of Na+ binding site in Na+ Pumping Rhodopsin (2016)
  • Author(s): S. Ito, S. Sugita, R. Abe-Yoshizumi, T. Iwata, K. Inoue, M. Iwaki, H. Kandori
  • Organizer: Gordon Research Seminar on "Photosensory Receptors & Signal Transduction"
  • Place of Presentation: Galveston, U.S.A.
  • Year and Date: 2016-01-23
  • Int'l Joint Research
• [Presentation] Optical activation of G-protein by microbial rhodopsin chimera (2016)
  • Author(s): K. Yoshida, T. Yamashita, R. Abe-Yoshizumi, M. Tanaka, K. Inoue, K. Sasaki, Y. Shichida, H. Kandori
  • Organizer: Gordon Research Seminar on "Photosensory Receptors & Signal Transduction"
  • Place of Presentation: Galveston, U.S.A.
  • Year and Date: 2016-01-23
  • Int'l Joint Research
• [Presentation] DTGロドプシンの光駆動イオン輸送とそのメカニズムの研究 (2015)
  • Author(s): 井上圭一、芝田洋平、鈴木悠斗、伊藤奨太、L. S. Brown、神取秀樹
  • Organizer: 日本生体エネルギー研究会第41回討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-12-21
• [Presentation] 真正細菌イオンポンプ型ロドプシンの機能転換とその光反応の解析 (2015)
  • Author(s): 野村祐梨香、井上圭一、神取秀樹
  • Organizer: 日本生体エネルギー研究会第41回討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-12-21
• [Presentation] Spectroscopic and structural studies on the sodium transporting mechanism of sodium pump rhodopsin (2015)
  • Author(s): K. Inoue, H. E. Kato, Y. Kato, R. Abe-Yoshizumi, O. Nureki, H. Kandori
  • Organizer: The 2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Optogenetic silencing of neuronal activity using a light-driven sodium ion pump in marine bacteria (2015)
  • Author(s): T. Ishizuka, S. Hososhima, M. R. Hoque, K. Yoshida, K. Inoue, H. Kandori, H. Yawo
  • Organizer: The 2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Novel group of eubacterial proton pumps with the unique proton-donating complex (2015)
  • Author(s): L. S. Brown, A. Harris, M. Ljumovic, S. Ito, K. Inoue, H. Kandori, A.-N. Bondar
  • Organizer: The 2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] The machinery of new eubacterial light-driven pumps: sodium and chloride pump rhodopsins (2015)
  • Author(s): K. Inoue, Y. Kato, A. Mori, R. Abe-Yoshizumi, H. Kandori
  • Organizer: The 2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 光でNa+イオンを運ぶ：Na+ポンプ型ロドプシンの発見とメカニズム研究 (2015)
  • Author(s): 井上圭一
  • Organizer: 金沢大学理工研究域公開セミナー
  • Place of Presentation: 金沢
  • Year and Date: 2015-12-11
  • Invited
• [Presentation] Toward optogenetics applications: microbial rhodopsin chimera (2015)
  • Author(s): K. Yoshida, T. Yamashita, R. Abe-Yoshizumi, M. Tanaka, K. Inoue, K. Sasaki, Y. Shichida, H. Kandori
  • Organizer: The 7th international symposium optogenetics 2015, Neural circuit and neuromodulation
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2015-12-04
  • Int'l Joint Research
• [Presentation] Spectroscopic study on the photo-reaction dynamics of sodium pump rhodopsin (2015)
  • Author(s): K. Inoue
  • Organizer: The seminar of the SFB 1078, Freie Universitat Berlin,
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2015-10-13
  • Int'l Joint Research / Invited
• [Presentation] Photochemistry of sodium pump rhodopsin (2015)
  • Author(s): K. Inoue
  • Organizer: DFG-Rundgesprach Photoreceptors
  • Place of Presentation: Frauenchiemsee, Germany
  • Year and Date: 2015-10-08
  • Int'l Joint Research / Invited
• [Presentation] Infrared spectroscopic study on the structure and dynamics of sodium pump rhodopsin (2015)
  • Author(s): K. Inoue
  • Organizer: The XVIth International Conference on Time-Resolved Vibrational Spectroscopy
  • Place of Presentation: Providence, U.S.A.
  • Year and Date: 2015-09-27
  • Int'l Joint Research / Invited
• [Presentation] 光駆動ナトリウムポンプの速度論的研究 (2015)
  • Author(s): 加藤善隆、井上圭一、吉住玲、神取秀樹
  • Organizer: 第9回分子科学討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-09-16
• [Presentation] タンパク質中で見られるチロシンの特異な水素結合状態の赤外分光解析 (2015)
  • Author(s): 伊藤奨太、杉田真也、吉住玲、井上圭一、岩田達也、岩城雅代、神取秀樹
  • Organizer: 第9回分子科学討論会
  • Place of Presentation: 東京
  • Year and Date: 2015-09-16
• [Presentation] The role of proton on the function of sodium pump rhodopsin (2015)
  • Author(s): K. Inoue
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research / Invited
• [Presentation] Role of Asn112 for transport activity by a light-driven sodium ion pump (2015)
  • Author(s): R. Abe-Yoshizumi, K. Inoue, H. E. Kato, O. Nureki, H. Kandori
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Engineering K+ pumping rhodopsin from KR2 (2015)
  • Author(s): M. Konno, K. Inoue, H. E. Kato, O. Nureki, H. Kandori
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Ion uptake mechanism of a light-driven Na+-H+ pump, KR2 (2015)
  • Author(s): Y. Kato, K. Inoue, R. Abe-Yoshizumi, H. Kandori
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Low-temperature FTIR study of sodium pumping rhodopsin" (2015)
  • Author(s): S. Ito, S. Sugita, R. Abe-Yoshizumi, K. Inoue, T. Iwata, H. Kandori
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Construction of chimeric proteins for optical control of Gs-protein activity (2015)
  • Author(s): K. Yoshida, K. Inoue, T. Yamashita, R. Abe-Yoshizumi, M. Tanaka, K. Sasaki, Y. Shichida, H. Kandori
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] A new proton pump rhodopsin without a conserved proton donor (2015)
  • Author(s): Y. Suzuki, K. Inoue, Y. Shibata, L. S. Brown, H. Kandori
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Single-molecule FRET studies on the light-induced structural changes of the sensory rhodopsin-transducer signaling complex (2015)
  • Author(s): R. Nishimura, K. Inoue, J. Yagasaki, K. Kawamoto, Y. Sudo, H. Kandori
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Functional conversion of eubacterial pump rhodopsins and the investigation of the photoreactions (2015)
  • Author(s): Y. Nomura, K. Inoue, H. Kandori
  • Organizer: The 53rd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Mechanisms of two different function on KR2 (2015)
  • Author(s): M. R. Hoque, S. Hososhima, K. Yoshida, T. Ishizuka, K. Inoue, H. Kandori, H. Yawo
  • Organizer: The 38th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2015-07-28
  • Int'l Joint Research
• [Presentation] Mechanisms of two different function on KR2 (2015)
  • Author(s): Y. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Hashimoto, H. Kandori
  • Organizer: 10th EBSA European Biophysics Congress
  • Place of Presentation: Dresden, Germany
  • Year and Date: 2015-07-18
  • Int'l Joint Research
• [Presentation] 時間分解分光法でせまる、タンパク質のイオン輸送メカニズム：光駆動型イオンポンプロドプシンの分光研究 (2015)
  • Author(s): 井上圭一
  • Organizer: 2015年先端光科学若手研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-14
  • Invited
• [Presentation] ナトリウムポンプ型ロドプシンのNa+輸送におけるNDQ-モチーフの役割についての分光研究 (2015)
  • Author(s): 井上圭一、加藤善隆、吉住 玲、神取秀樹
  • Organizer: 第42回生体分子科学討論会
  • Place of Presentation: 高崎
  • Year and Date: 2015-06-12
• [Presentation] 真正細菌光駆動型イオンポンプにおけるCl-ポンプとH+ポンプの機能転換 (2015)
  • Author(s): 野村祐梨香、井上圭一、神取秀樹
  • Organizer: 第42回生体分子科学討論会
  • Place of Presentation: 高崎
  • Year and Date: 2015-06-12
• [Presentation] 光駆動ナトリウムイオンポンプの超高速光反応ダイナミクス (2015)
  • Author(s): 田原進也、竹内 佐年、吉住怜、井上圭一、神取秀樹、田原太平
  • Organizer: 第42回生体分子科学討論会
  • Place of Presentation: 高崎
  • Year and Date: 2015-06-12
• [Presentation] 細菌の走性における数的多様性の解明 (2015)
  • Author(s): 井上圭一
  • Organizer: 新学術領域「少数性生物学」第9回領域会議
  • Place of Presentation: 沖縄
  • Year and Date: 2015-06-05
• [Presentation] Microbial rhodopsins: Light-driven biological proton, chloride and sodium transporters (2015)
  • Author(s): K. Inoue
  • Organizer: BIT's 4th Annual World Congress of Advanced Materials-2015
  • Place of Presentation: Chongqing, China
  • Year and Date: 2015-05-27
  • Int'l Joint Research / Invited
• [Presentation] 微生物型ロドプシンを用いた三量体Gtタンパク質の光操作 (2015)
  • Author(s): 井上圭一、佐々木賢吾、山下高廣、吉田一帆、七田芳則、神取秀樹
  • Organizer: 第12回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-15
• [Presentation] 光受容キメラタンパク質によるGタンパク質の光制御 (2015)
  • Author(s): 吉田一帆、井上圭一、山下高廣、吉住玲、田中瑞奈、佐々木賢吾、七田芳則、神取秀樹
  • Organizer: 第12回GPCR研究会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-15
• [Presentation] H+ポンプとNa+ポンプ：２つのロドプシンから見えるもの (2015)
  • Author(s): 井上圭一
  • Organizer: 分子研研究会「膜タンパク質内部のプロトン透過を考える」
  • Place of Presentation: 岡崎
  • Year and Date: 2015-04-20
  • Invited
• [Presentation] 光駆動ナトリウムイオンポンプのイオン取込みと選択性 (2015)
  • Author(s): 加藤善隆、井上圭一、吉住 玲、橋本優一、神取秀樹
  • Organizer: 分子研研究会「膜タンパク質内部のプロトン透過を考える」
  • Place of Presentation: 岡崎
  • Year and Date: 2015-04-20
• [Presentation] カリウムイオンをポンプするロドプシンの創成 (2015)
  • Author(s): 今野雅恵、井上圭一、加藤英明、濡木 理、神取秀樹
  • Organizer: 分子研研究会「膜タンパク質内部のプロトン透過を考える」
  • Place of Presentation: 岡崎
  • Year and Date: 2015-04-20
• [Presentation] H+ポンプ型ロドプシンAR3のH+チャネルへの機能転換 (2015)
  • Author(s): 鈴木悠斗、井上圭一、林 重彦、須藤雄気、神取秀樹
  • Organizer: 分子研研究会「膜タンパク質内部のプロトン透過を考える」
  • Place of Presentation: 岡崎
  • Year and Date: 2015-04-20
• [Presentation] 真正細菌ロドプシンのナトリウムポンプ、クロライドポンプからプロトンポンプへの機能転換 (2015)
  • Author(s): 野村祐梨香、井上圭一、神取秀樹
  • Organizer: 分子研研究会「膜タンパク質内部のプロトン透過を考える」
  • Place of Presentation: 岡崎
  • Year and Date: 2015-04-20
• [Presentation] 光駆動ナトリウムポンプKR2のポンプスイッチにおけるAsn112の重要性 (2015)
  • Author(s): 吉住 玲、井上圭一、加藤英明、濡木 理、神取秀樹
  • Organizer: 分子研研究会「膜タンパク質内部のプロトン透過を考える」
  • Place of Presentation: 岡崎
  • Year and Date: 2015-04-20
• [Presentation] Spectroscopic study on the dynamics and structure of sodium pump rhodopsin (2015)
  • Author(s): Keiichi Inoue
  • Organizer: 日本化学会第94春季年会(2014)
  • Place of Presentation: Funabashi, Japan
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] 細菌の走性における数的多様性の解明 (2015)
  • Author(s): 井上圭一
  • Organizer: 新学術領域研究「少数性生物学」第8回領域会議
  • Place of Presentation: 北海道
  • Year and Date: 2015-01-31
• [Presentation] 光駆動ナトリウムポンプ型ロドプシンの発見と輸送メカニズム (2014)
  • Author(s): 井上圭一
  • Organizer: 機能物性融合科学研究会シリーズ(1)「光機能」
  • Place of Presentation: 柏
  • Year and Date: 2014-12-04
  • Invited
• [Presentation] Microbial rhodopsins of marine bacteria: Nano-scale biological light-driven ion pumps (2014)
  • Author(s): Keiichi Inoue
  • Organizer: 25th 2014 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2014-11-12
  • Invited
• [Presentation] Function and mechanism of sodium pump rhodopsin (2014)
  • Author(s): Keiichi Inoue
  • Organizer: 16th International Conference on Retinal Proteins
  • Place of Presentation: Nagahama, Japan
  • Year and Date: 2014-10-07
  • Invited
• [Presentation] Spectroscopic Study of a Light-Driven Chloride Ion Pump from Marine Bacteria (2014)
  • Author(s): Keiichi Inoue, Faisal Hammad Mekky Koua, Yoshitaka Kato, Rei Abe-Yoshizumi, Hideki Kandori
  • Organizer: 16th International Conference on Retinal Proteins
  • Place of Presentation: Nagahama, Japan
  • Year and Date: 2014-10-06 – 2014-10-09
• [Presentation] The Extracellular pH Dependency of Transport Activity by Light-driven Sodium Ion Pump (2014)
  • Author(s): Rei Abe-Yoshizumi, Yoshitaka Kato, Keiichi Inoue, Hideki Kandori
  • Organizer: The 52nd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2014-09-27
• [Presentation] Role of D116 for the Na+ Pump Activity and Molecular Property of NdR2 (2014)
  • Author(s): Shinya Sugita, Keiichi Inoue, Rei Abe-Yoshizumi, Yoshitaka Kato, Hideki Kandori
  • Organizer: The 52nd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2014-09-27
• [Presentation] Functional Restoration of a Pseudo Gene of Rhodopsin with NDQ Motif (2014)
  • Author(s): Yuto Suzuki, Keiichi Inoue, Rei Abe-Yoshizumi, Hideki Kandori
  • Organizer: The 52nd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2014-09-25
• [Presentation] Single-molecule FRET study of the sensory rhodopsin I – transducer complex (2014)
  • Author(s): Ryo Nishimura, Keiichi Inoue, Jin Yagasaki, Kenichi Kawamoto, Yuki Sudo, Hideki Kandori
  • Organizer: The 52nd Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2014-09-25
• [Presentation] Optical Control of the Gs-protein Activity by Microbial Rhodopsin Chimeras (2014)
  • Author(s): Kazuho Yoshida, Keiichi Inoue, Takahiro Yamashita, Rei Abe-Yoshizumi, Kengo Sasaki, Yoshinori Shichida, Hideki Kandori
  • Organizer: 16th International Congress of Photobiology
  • Place of Presentation: Córdoba, Argentina
  • Year and Date: 2014-09-09
• [Presentation] 細菌の走性における数的多様性の解明 (2014)
  • Author(s): 井上圭一
  • Organizer: 新学術領域研究「少数性生物学」第7回領域会議
  • Place of Presentation: 大津
  • Year and Date: 2014-06-21
• [Presentation] 光駆動型プロトンポンプから光ゲート型チャネルへの機能転換 (2014)
  • Author(s): 井上圭一、下野和実、鈴木悠斗、谷ヶ崎仁、宮内正二、林重彦、神取秀樹、須藤雄気
  • Organizer: 第41回生体分子科学討論会
  • Place of Presentation: 福岡
  • Year and Date: 2014-06-07
• [Presentation] The Sodium Pump Rhodopsin Discovered in Ocean (2014)
  • Author(s): Keiichi Inoue, Hikaru Ono, Rei Abe-Yoshizumi, Yoshitaka Kato, Hiroyasu Ito, Susumu Yoshizawa, Kazuhiro Kogure, Hideki Kandori
  • Organizer: Gordon Research Conference on “Photosensory Receptors & Signal Transduction”
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2014-04-09
• [Book] 光異性化と生命現象 光異性化と生命現象 (2016)
  • Author(s): 井上圭一
  • Publisher: 朝倉書店
• [Remarks] 名古屋工業大学-研究者詳細-井上 圭一
  • URL: http://researcher.nitech.ac.jp/html/14_ja.html
• [Remarks] 名古屋工業大学・研究者データベースシステム
  • URL: http://researcher.nitech.ac.jp/html/14_ja.html?l=ja&k=%E4%BA%95%E4%B8%8A&o=name-a&p=1
• [Remarks] 名古屋工業大学神取研究室・研究業績
  • URL: http://www.ach.nitech.ac.jp/~physchem/kandori/index_j.html