長期報酬記憶を制御するフィードバック神経回路

• Project Area: The Science of Mental Time: investigation into the past, present, and future
• Project/Area Number: 26119503
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Tohoku University
• Principal Investigator: 谷本 拓 東北大学, 生命科学研究科, 教授 (70714955)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 神経科学

Outline of Annual Research Achievements

短期記憶・長期記憶などに分類されるように、生理学的に異なったプロセスを経て形成される記憶の成分比は、時間経過に伴って変化する。特に長期記憶は学習後一定時間経ってから形成され始めるなど、その形成過程には謎が多い。これを構成する神経細胞がいつ、どのように働くかの解明は、神経回路の発現する機能を理解するうえで不可欠である。我々は先行研究で、短期報酬記憶と長期報酬記憶は独立して形成されることを示した。さらに、長期報酬記憶を特異的に誘導するドーパミン細胞種を同定した。このドーパミン神経細胞は、昆虫の連合記憶中枢であるキノコ体の特定領域に入力する。本研究では、この領域からの出力神経が、ドーパミン入力神経に再び入力するというフィードバック回路を形成しているという仮説に基づき、その形態の詳細な解析を行った。
平成27年度は記憶の形成、固定化など様々なタイミングで強制的にフィードバック回路の機能阻害または活性化を行い、各時点におけるその重要性を調査した。この結果、これに関わる神経回路に機能不全を持つハエは長期記憶を形成できないことが分かった。すなわち、報酬情報がキノコ体のフィードバック回路によって保持され、安定した長期記憶の形成に重要な役割を担うということが証明された。この成果は、Ichinose et al., eLife, 2015に代表される4編の原著論文として報告した。

Research Progress Status

27年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

27年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Max Planck Institute of Neurobiology/University of Munich(Germany)
• [Int'l Joint Research] HHMI Janelia Research Campus/Cold Spring Harbor Laboratory(米国)
• [Journal Article] Direct neural pathways convey distinct visual information to Drosophila mushroom bodies. (2016)
  • Author(s): Vogt K, Aso Y, Hige T, Knapek S, Ichinose T, Friedrich AB, Turner GC, Rubin GM, Tanimoto H.
  • Journal Title: eLife Volume: in press
  • DOI: 10.7554/elife.14009
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functional dissociation in sweet taste receptor neurons between and within taste or- gans of Drosophila. (2016)
  • Author(s): Thoma V, Knapek S, Arai S, Hartl M, Kohsaka H, Sirigrivatanawong P, Abe A, Hashimoto K, Tanimoto H.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 10678-10678
  • DOI: 10.1038/ncomms10678
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reward signal in a recurrent circuit drives appetitive long-term memory formation. (2015)
  • Author(s): Ichinose T, Aso Y, Yamagata N, Abe A, Rubin GM, Tanimoto H.
  • Journal Title: eLife Volume: 4 Pages: 1-18
  • DOI: 10.7554/elife.10719
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reversing Stimulus Timing in Visual Conditioning Leads to Memories with Opposite Valence in Drosophila. (2015)
  • Author(s): Vogt K, Yarali A, Tanimoto H.
  • Journal Title: PLoS One Volume: 10(10) Issue: 10 Pages: e0139797-e0139797
  • DOI: 10.1371/journal.pone.0139797
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Distinct dopamine neurons mediate reward signals for short- and long-term memories (2015)
  • Author(s): Yamagata N, Ichinose T, Aso Y, Placais P, Friedrich AB, Sima RJ, Preat T, Rubin GM, Tanimoto H
  • Journal Title: PNAS Volume: 112 Issue: 2 Pages: 578-583
  • DOI: 10.1073/pnas.1421930112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila (2014)
  • Author(s): Aso Y, Sitaraman D, Ichinose T, Kaun KR, Vogt K, Belliart-Guerin G, Placais PY, Robie AA, Yamagata N, Schnaitmann C, Rowell WJ, Johnston RM, Ngo TT, Chen N, Korff W, Nitabach MN, Heberlein U, Preat T, Branson KM, Tanimoto H, Rubin GM
  • Journal Title: Elife Volume: 4
  • DOI: 10.7554/elife.04580
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The neuronal architecture of the mushroom body provides a logic for associative learning. (2014)
  • Author(s): Aso Y, Hattori D, Yu Y, Johnston R, Iyer N, Ngo T, Dionne H, Abbott L, Axel R, Tanimoto H, Rubin G.
  • Journal Title: eLife Volume: 3
  • DOI: 10.7554/elife.04577
  • Peer Reviewed / Open Access
• [Journal Article] Shared mushroom body circuits operate visual and olfactory memories in Drosophila. (2014)
  • Author(s): Vogt K, Schnaitmann C, Dylla K, Knapek S, Aso Y, Rubin GM, Tanimoto H.
  • Journal Title: eLife Volume: 3
  • Peer Reviewed / Open Access
• [Presentation] Neural pathways for the formation, consolidation, and retrieval of memory in the fly brain. (2016)
  • Author(s): Tanimoto H.
  • Organizer: The 6th International Symposium on "Biology of Decision Making"
  • Place of Presentation: Ecole Normale Superieure & Institut du Cerveau et de la Moelle epiniere, Paris (France)
  • Year and Date: 2016-05-25
  • Int'l Joint Research / Invited
• [Presentation] Dopamine circuits and memory formation. (2015)
  • Author(s): Tanimoto H.
  • Organizer: 14th European Symposium for Insect Taste and Olfaction
  • Place of Presentation: Residence Hotel Cormoran, Cagliari (Italy)
  • Year and Date: 2015-09-20
  • Int'l Joint Research / Invited
• [Presentation] Mushroom body reward circuits. (2015)
  • Author(s): Tanimoto H.
  • Organizer: The EMBO-Kavli Workshop on Neural Circuits and Behaviour of Drosophila
  • Place of Presentation: Orthodox academy of Crete, Hania (Greece)
  • Year and Date: 2015-07-05
  • Int'l Joint Research / Invited
• [Presentation] Dopamine circuits for memory formation. (2015)
  • Author(s): Tanimoto H.
  • Organizer: 3rd Asia-Pacific Drosophila Research Conference
  • Place of Presentation: Beijing Conference Center, Beijing (China)
  • Year and Date: 2015-05-11
  • Int'l Joint Research / Invited
• [Presentation] Mashroom body circuits and memory formation. (2014)
  • Author(s): Tanimoto H.
  • Organizer: The Genetics Society Autumn Meeting 2014
  • Place of Presentation: The Royal Society, London (UK)
  • Year and Date: 2014-11-27 – 2014-11-28
  • Invited
• [Presentation] Mashroom body circuits and memory formation. (2014)
  • Author(s): Tanimoto H.
  • Organizer: Neurofly 2014
  • Place of Presentation: Convention Center Crete Maris, Crete (Greece)
  • Year and Date: 2014-10-05 – 2014-10-09
  • Invited
• [Presentation] Distinct dopamine neurons mediate reward signals for short- and long-term memories. (2014)
  • Author(s): Tanimoto H.
  • Organizer: Janelia Farm 2014 Fall Conference
  • Place of Presentation: Janelia Farm Reserch Campus, Ashburn (USA)
  • Year and Date: 2014-09-21 – 2014-09-24
  • Invited
• [Presentation] Neural circuits for memory formation. (2014)
  • Author(s): Tanimoto H.
  • Organizer: The 85th Annual Meeting of the Zoological Society of Japan
  • Place of Presentation: Tohoku University Kawauchi-kita Campus, Sendai
  • Year and Date: 2014-09-11 – 2014-09-13
  • Invited
• [Presentation] Neural circuits for colour discrimination and learning. (2014)
  • Author(s): Tanimoto H, Vogt K, Schnaitmann C, Aso Y, Rubin GM, Wachtler T, Garbers C.
  • Organizer: 11th International Congress of Neuroethology and 36th Annual Meeting of The Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: Sapporo Convention Center, Sapporo
  • Year and Date: 2014-07-29 – 2014-08-01
  • Invited
• [Presentation] Functional diversity of the Drosophila mushroom body. (2014)
  • Author(s): Tanimoto H.
  • Organizer: Janelia Farm 2014 Spring Conference
  • Place of Presentation: Janelia Farm Reserch Campus, Ashburn (USA)
  • Year and Date: 2014-04-27 – 2014-05-01
  • Invited