細胞機能を司るオルガネラ・ゾーンの解読

• Project Area: Toward an integrative understanding of functional zones in organelles
• Project/Area Number: 22H04899
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: 清水 重臣 東京医科歯科大学, 難治疾患研究所, 教授 (70271020)
• Project Period (FY): 2022-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2022: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: オルガネラ / ミトコンドリア / ゴルジ体 / 小胞体

Outline of Research at the Start

５年間の以下に示す研究成果に関して、資料を整理し、冊子体の報告書としてまとめ、国内の大学や研究機関ならびに関連する研究者に送付する予定である。即ち、（１）計画研究班および公募班の研究成果、（２）総括班による研究支援の実績と作製された研究資材のまとめ、（３）国内シンポジウムおよび国際シンポジウム、（４）共同研究、（５）若手研究支援および各班の構成員のプロモーションの状況、（６）広報およびアウトリーチ活動に関して、とりまとめを行う。

Outline of Annual Research Achievements

オルガネラ・ゾーン研究を継続する上で、研究者間の連携を維持すること、情報交換を行うことを目的に、オルガネラゾーンセミナーを毎月オンラインで開催し、2023年3月までに通算21回のオンラインセミナーを開催した。これを基盤として、領域内外の研究者との議論、情報の相互交換、共同研究の推進を図ることができた。
また、新型コロナ感染症の影響で前年度に開催できなかった国際シンポジウムを立教大学において開催し、オルガネラ研究の第一人者であるPietro De Camilli教授、Alissa M Weaver教授、Jaakko Saraste教授、Byung Il Lee教授、Fabien Alpy教授を含めて、約100名の国内外の研究者が参加した。同シンポジウムでは、招聘した教授からの講演を聴取するとともに、最新のオルガネラ解析技術などに関する意見交換を行い、共同研究を打ち合わせた。
さらに、各計画班、公募班の先生方からの情報提供を受けて、５年間の領域の研究を総括を行うとともに、事後評価報告書の作成を行なった。また、報告書に対するフィードバックを行い、オルガネラゾーン研究の更なる研究の進捗を図った。

Research Progress Status

令和4年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和4年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Penn State University/Washington University/Harvard University(米国)
• [Int'l Joint Research] Yonsei University(韓国)
• [Int'l Joint Research] Barcelona University(スペイン)
• [Journal Article] Absence of ULK1 decreases AMPK activity in the kidney, leading to chronic kidney disease progression (2023)
  • Author(s): Tomoki Yanagi, Hiroaki Kikuchi, Koichiro Susa, Naohiro Takahashi, Naohiro Takahashi, Hiroki Bamba, Takefumi Suzuki, Yuta Nakano, Tamami Fujiki, Yutaro Mori, Fumiaki Ando, Shintaro Mandai, Takayasu Mori, Koh Takeuchi, Shinya Honda, Satoru Torii, Shigeomi Shimizu, Tatemitsu Rai, Shinichi Uchida, Eisei Sohara
  • Journal Title: Genes to Cells Volume: 28 Issue: 1 Pages: 5-14
  • DOI: 10.1111/gtc.12989
  • Peer Reviewed / Open Access
• [Journal Article] Nickel particles are present in Crohn's disease tissue and exacerbate intestinal inflammation in IBD susceptible mice. (2022)
  • Author(s): Hiroki Matsuda, Yoichi Nibe-Shirakihara, Akiko Tamura, Emi Aonuma, Satoko Arakawa, Kana Otsubo, Yasuhiro Nemoto, Takashi Nagaishi, Kiichiro Tsuchiya, Shigeomi Shimizu, Averil Ma, Mamoru Watanabe, Motohiro Uo, Ryuichi Okamoto, Shigeru Oshima.
  • Journal Title: Biochemical and Biophysical Research Communications. Volume: 592 Pages: 74-80
  • DOI: 10.1016/j.bbrc.2021.12.111
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] FLIP-based autophagy-detecting technique reveals closed autophagic compartments (2022)
  • Author(s): Sakurai Hajime Tajima、Arakawa Satoko、Noguchi Saori、Shimizu Shigeomi
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 22452-22452
  • DOI: 10.1038/s41598-022-26430-5
  • Peer Reviewed / Open Access
• [Journal Article] Nanaomycin E inhibits NLRP3 inflammasome activation by preventing mitochondrial dysfunction (2022)
  • Author(s): Matsui Yudai、Takemura Naoki、Shirasaki Yoshitaka、Takahama Michihiro、Noguchi Yoshihiko、Ikoma Kenta、Pan Yixi、Nishida Shuhei、Taura Manabu、Nakayama Akiyoshi、Funatsu Takashi、Misawa Takuma、Harada Yoshie、Sunazuka Toshiaki、Saitoh Tatsuya
  • Journal Title: International Immunology Volume: 34 Issue: 10 Pages: 505-518
  • DOI: 10.1093/intimm/dxac028
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Oridonin suppresses particulate-induced NLRP3-independent IL-1α release to prevent crystallopathy in the lung. (2022)
  • Author(s): Ikoma K, Takahama M, Kimishima A, Pan Y, Taura M, Nakayama A, Arai M, Takemura N, Saitoh T.
  • Journal Title: Int Immunol. Volume: 34 Issue: 10 Pages: 493-504
  • DOI: 10.1093/intimm/dxac018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection (2022)
  • Author(s): Liu Chun-chun、Liu Ya-yun、Zhou Jiang-fei、Chen Xi、Chen Huan、Hu Jia-huan、Chen Jing、Zhang Jin、Sun Rui-cong、Wei Jian-chao、Go Yun Young、Morita Eiji、Zhou Bin
  • Journal Title: PLOS Pathogens Volume: 18 Issue: 2 Pages: 1010294-1010294
  • DOI: 10.1371/journal.ppat.1010294
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of Nuclear Envelope Stress on Physiological and Pathological Processes in Central Nervous System (2022)
  • Author(s): Kamikawa Yasunao、Saito Atsushi、Imaizumi Kazunori
  • Journal Title: Neurochemical Research Volume: 47 Issue: 9 Pages: 2478-2487
  • DOI: 10.1007/s11064-022-03608-x
• [Journal Article] Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide (2022)
  • Author(s): Goto A, Sakai s, Mizuike A, Yamaji T, Hanada K
  • Journal Title: iScience Volume: 25 Issue: 7 Pages: 164624-164624
  • DOI: 10.1016/j.isci.2022.104624
  • Peer Reviewed / Open Access
• [Journal Article] Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering Throughout the Endoplasmic Reticulum (2022)
  • Author(s): Shimasaki K, Kumagai K, Sakai S, Yamaji T, Hanada K
  • Journal Title: Int J Mol Sci Volume: 23 Issue: 7 Pages: 4025-4025
  • DOI: 10.3390/ijms23074025
  • Peer Reviewed / Open Access
• [Journal Article] Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling (2022)
  • Author(s): Moriyama Hidenori、Endo Jin、Kataoka Masaharu、Shimanaka Yuta、Kono Nozomu、他13人、Arai Hiroyuki、Sano Motoaki
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 3013-3013
  • DOI: 10.1038/s41467-022-30621-z
  • Peer Reviewed / Open Access
• [Journal Article] Role of the orphan transporter SLC35E1 in the nuclear egress of herpes simplex virus 1 (2022)
  • Author(s): Fumio Maeda, Akihisa Kato, Kosuke Takeshima, Misato Shibazaki, Ryota Sato, Takuma Shibata, Kensuke Miyake, Hiroko Kozuka-Hata, Masaaki Oyama, Eigo Shimizu, Seiya Imoto, Satoru Miyano, Shungo Adachi, Tohru Natsume, Koh Takeuchi, Yuhei Maruzuru, Naoto Koyanagi, Jun Arii, Yasushi Kawaguchi
  • Journal Title: Journal of Virology Volume: - Issue: 10 Pages: 10-10
  • DOI: 10.1128/jvi.00306-22
  • Peer Reviewed / Open Access
• [Journal Article] Hippo-TAZ signaling is the master regulator of the onset of triple-negative basal-like breast cancers. (2022)
  • Author(s): Soyama H, Nishio M, Otani J, Sakuma T, Takao S, Hara S, Masuda T, Mimori K, Toyokuni S, Lydon JP, Nakao K, Nishina H, Fukumoto T, Maehama T, Suzuki A.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 119 Issue: 29 Pages: 1-10
  • DOI: 10.1073/pnas.2123134119
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Compartment-specific tuning of dendritic feature selectivity by intracellular Ca ²⁺ release (2022)
  • Author(s): O’Hare Justin K.、Gonzalez Kevin C.、Herrlinger Stephanie A.、Hirabayashi Yusuke、Hewitt Victoria L.、Blockus Heike、Szoboszlay Miklos、Rolotti Sebi V.、Geiller Tristan C.、Negrean Adrian、Chelur Vikas、Polleux Franck、Losonczy Attila
  • Journal Title: Science Volume: 375 Issue: 6586 Pages: 382-382
  • DOI: 10.1126/science.abm1670
• [Journal Article] GET pathway mediates transfer of mislocalized tail-anchored proteins from mitochondria to the ER (2022)
  • Author(s): Matsumoto Shunsuke、Ono Suzuka、Shinoda Saori、Kakuta Chika、Okada Satoshi、Ito Takashi、Numata Tomoyuki、Endo Toshiya
  • Journal Title: Journal of Cell Biology Volume: 221 Issue: 6 Pages: 202-202
  • DOI: 10.1083/jcb.202104076
  • Peer Reviewed / Open Access
• [Journal Article] Quality-controlled ceramide-based GPI-anchored protein sorting into selective ER exit sites (2022)
  • Author(s): Rodriguez-Gallardo Sofia、Sabido-Bozo Susana、Ikeda Atsuko、Araki Misako、Okazaki Kouta、Nakano Miyako、Aguilera-Romero Auxiliadora、Cortes-Gomez Alejandro、Lopez Sergio、Waga Miho、Nakano Akihiko、Kurokawa Kazuo、Muniz Manuel、Funato Kouichi
  • Journal Title: Cell Reports Volume: 39 Issue: 5 Pages: 110768-110768
  • DOI: 10.1016/j.celrep.2022.110768
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis (2022)
  • Author(s): Yoko Hasegawa, Thais Huarancca Reyes, Tomohiro Uemura, Anirban Baral, Akari Fujimaki, Yongming Luo, Yoshie Morita, Yasushi Saeki, Shugo Maekawa, Shigetaka Yasuda, Koki Mukuta, Yoichiro Fukao, Keiji Tanaka, Akihiko Nakano, Junpei Takagi, Rishikesh P Bhalerao, Junji Yamaguchi, Takeo Sato
  • Journal Title: THE PLANT CELL Volume: 34 Issue: 4 Pages: 1354-1374
  • DOI: 10.1093/plcell/koac014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CDP-ribitol prodrug treatment ameliorates ISPD-deficient muscular dystrophy mouse model (2022)
  • Author(s): Tokuoka Hideki、Imae Rieko、Nakashima Hitomi、Manya Hiroshi、Masuda Chiaki、Hoshino Shunsuke、Kobayashi Kazuhiro、Lefeber Dirk J.、Matsumoto Riki、Okada Takashi、Endo Tamao、Kanagawa Motoi、Toda Tatsushi
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 1847-1847
  • DOI: 10.1038/s41467-022-29473-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome-wide CRISPR screen reveals CLPTM1L as a lipid scramblase required for efficient glycosylphosphatidylinositol biosynthesis (2022)
  • Author(s): Wang Yicheng、Menon Anant K.、Maki Yuta、Liu Yi-Shi、Iwasaki Yugo、Fujita Morihisa、Guerrero Paula A.、Silva Daniel Varon、Seeberger Peter H.、Murakami Yoshiko、Kinoshita Taroh
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 119 Issue: 14 Pages: 7211-7211
  • DOI: 10.1073/pnas.2115083119
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] O-GlcNAcylation Regulates β1,4-GlcNAc-branched N-glycan Biosynthesis Via the OGT/SLC35A3/GnT-IV Axis. (2022)
  • Author(s): Song, W., Isaji, T., Nakano, M., Liang C., Fukuda, T.and Gu, J.
  • Journal Title: FASEB J. Volume: 36 Issue: 2 Pages: 210-210
  • DOI: 10.1096/fj.202101520r
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Biological roles of GOMED (2023)
  • Author(s): Shigeomi Shimizu
  • Organizer: Organellar Zone International Symposium
  • Int'l Joint Research / Invited
• [Remarks] オルガネラゾーン ホームページ
  • URL: https://www.tmd.ac.jp/organellezone/index.html
• [Funded Workshop] Organellar Zone International Symposium (2023)