植物の成長可塑性を支える環境認識と記憶の自律分散型統御システム

2019 Fiscal Year Annual Research Report

• Project Area: Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
• Project/Area Number: 15H05955
• Research Institution: Nagoya University
• Principal Investigator: 木下 俊則 名古屋大学, 理学研究科(WPI), 教授 (50271101)
• Co-Investigator(Kenkyū-buntansha): 松林 嘉克 名古屋大学, 理学研究科, 教授 (00313974) ; 福田 裕穂 東京大学, 大学院理学系研究科(理学部), 特任教授 (10165293) ; 角谷 徹仁 東京大学, 大学院理学系研究科(理学部), 教授 (20332174) ; 白須 賢 国立研究開発法人理化学研究所, 環境資源科学研究センター, グループディレクター (20425630) ; 篠崎 和子 東京大学, 大学院農学生命科学研究科(農学部), 教授 (30221295) ; 杉本 慶子 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (30455349) ; 松永 幸大 東京理科大学, 理工学部応用生物科学科, 教授 (40323448) ; 多田 安臣 名古屋大学, 遺伝子実験施設, 教授 (40552740) ; 鈴木 孝征 中部大学, 応用生物学部, 准教授 (50535797) ; 望田 啓子 (桑田啓子) 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (70624352)
• Project Period (FY): 2015-06-29 – 2020-03-31
• Keywords: 植物 / 環境応答 / 次世代シークエンサー / 質量分析 / イメージング / in vitroタンパク質合成

Outline of Annual Research Achievements

（１）連携研究の推進：班員間の連携と共同研究の推進を図るため、領域会議や若手の会に加え、計画代表間でインターネット会議等による月１回以上の綿密な情報交換や年１回以上のジョイントラボミーティングを行った。また、領域会議や若手の会の際に、テクニカルセミナーを行い、各班の持つ研究技術を紹介し、領域内での効率的な研究推進、研究班間の共同研究の促進を図った。
（２）研究支援センターの運営：最先端での研究推進には、高度な技術もった専門者による研究機器・研究技術の利用が必須である。本領域では、総括班に研究支援センター（次世代シークエンス部門、質量分析部門、イメージング部門、in vitroタンパク質合成部門）を設置し、その活動を通じて、領域全体の研究を加速させた。
（３）国際連携拠点の設置・若手研究者育成：国際的な研究ネットワーク体制構築を積極的に進めるため、英国John Innes Centre・The Sainsbury Laboratory、米国Stanford Universityに海外共同研究拠点を設け、総括班に配置する国際企画、国際連携コーディネーターを中心として、合同戦略会議の開催や国際共同研究・情報交換、国際シンポジウムを積極的に推進した。また、第５回若手の会を開催し（2019年9月25日-27日、御殿場高原　時之栖）、研究発表のみならず、国立遺伝学研究所の藤田智史博士と名古屋大学ITbMの打田直行博士により、これまでの研究人生で学んだことや重要な決断をどうするかなどを、面白く、赤裸々に語って頂く特別講演も行い、本領域に参加する若手研究者の育成に務めた。
（４）社会・国民への情報発信：研究成果はプレスリリース、領域ホームページや領域の広報活動などを通じ国民に紹介した。また、一般者や中高生向けのアウトリーチを積極的に行い、本領域研究の意義や成果を分かりやすく伝えた。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] The Sainsbury laboratory/John Innes Centre(英国)
  • Country Name: UNITED KINGDOM
  • Counterpart Institution: The Sainsbury laboratory/John Innes Centre
• [Int'l Joint Research] 北京大学/上海交通大学/南京農業大学(中国)
  • Country Name: CHINA
  • Counterpart Institution: 北京大学/上海交通大学/南京農業大学
• [Int'l Joint Research] ミラン大学(イタリア)
  • Country Name: ITALY
  • Counterpart Institution: ミラン大学
• [Int'l Joint Research] UCSD/ワシントン大学(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: UCSD/ワシントン大学
• [Journal Article] Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis (2020)
  • Author(s): Ota Ryosuke、Ohkubo Yuri、Yamashita Yasuko、Ogawa-Ohnishi Mari、Matsubayashi Yoshikatsu
  • Journal Title: Nature Communications Volume: 11 Pages: 641(9pages)
  • DOI: 10.1038/s41467-020-14440-8
  • Peer Reviewed / Open Access
• [Journal Article] Two combinatorial patterns of telomere histone marks in plants with canonical and non‐canonical telomere repeats (2020)
  • Author(s): Adamusov? Kate?ina、Khosravi Solmaz、Fujimoto Satoru、Houben Andreas、Matsunaga Sachihiro、Fajkus Ji??、Fojtov? Miloslava
  • Journal Title: The Plant Journal Volume: 102 Pages: 678～687
  • DOI: 10.1111/tpj.14653
  • Peer Reviewed / Open Access
• [Journal Article] Deep Imaging Analysis in VISUAL Reveals the Role of YABBY Genes in Vascular Stem Cell Fate Determination (2020)
  • Author(s): Nurani Alif Meem、Ozawa Yasuko、Furuya Tomoyuki、Sakamoto Yuki、Ebine Kazuo、Matsunaga Sachihiro、Ueda Takashi、Fukuda Hiroo、Kondo Yuki
  • Journal Title: Plant and Cell Physiology Volume: 61 Pages: 255～264
  • DOI: 10.1093/pcp/pcaa002
  • Peer Reviewed / Open Access
• [Journal Article] AT-Hook Transcription Factors Restrict Petiole Growth by Antagonizing PIFs (2020)
  • Author(s): Favero David S.、Kawamura Ayako、Shibata Michitaro、Takebayashi Arika、Jung Jae-Hoon、Suzuki Takamasa、Jaeger Katja E.、Ishida Takashi、Iwase Akira、Wigge Philip A.、Neff Michael M.、Sugimoto Keiko
  • Journal Title: Current Biology Volume: 30 Pages: 1454～1466.e6
  • DOI: 10.1016/j.cub.2020.02.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of stomatal opening and histone modification by photoperiod in Arabidopsis thaliana (2019)
  • Author(s): Aoki Saya、Toh Shigeo、Nakamichi Norihito、Hayashi Yuki、Wang Yin、Suzuki Takamasa、Tsuji Hiroyuki、Kinoshita Toshinori
  • Journal Title: Scientific Reports Volume: 9 Pages: 10054(9pages)
  • DOI: 10.1038/s41598-019-46440-0
  • Peer Reviewed / Open Access
• [Journal Article] Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock (2019)
  • Author(s): Uehara, T. N., Mizutani, Y., Kuwata, K., Hirota, T., Sato, A., Mizoi, J., Takao, S., Matsuo, H., Suzuki, T., Ito, S., Saito, A. N., Nishiwaki-Ohkawa, T., Yamaguchi-Shinozaki, K., Yoshimura, T,. Kay, S. A., Itami, K., Kinoshita, T., *Yamaguchi, J., and Nakamichi, N.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 116 Pages: 11528～11536
  • DOI: 10.1073/pnas.1903357116
  • Peer Reviewed / Open Access
• [Journal Article] Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization (2019)
  • Author(s): Oochi Akihiro、Hajny Jakub、Fukui Kosuke、Nakao Yukio、Gallei Michelle、Quareshy Mussa、Takahashi Koji、Kinoshita Toshinori、Harborough Sigurd Ramans、Kepinski Stefan、Kasahara Hiroyuki、Napier Richard、Friml Ji??、Hayashi Ken-ichiro
  • Journal Title: Plant Physiology Volume: 180 Pages: 1152～1165
  • DOI: 10.1104/pp.19.00201
  • Peer Reviewed / Open Access
• [Journal Article] AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development (2019)
  • Author(s): Motomura Kazuki、Arae Toshihiro、Araki-Uramoto Haruka、Suzuki Yuya、Takeuchi Hidenori、Suzuki Takamasa、Ichihashi Yasunori、Shibata Arisa、Shirasu Ken、Takeda Atsushi、Higashiyama Tetsuya、Chiba Yukako
  • Journal Title: Plant and Cell Physiology Volume: 61 Pages: 712～721
  • DOI: 10.1093/pcp/pcz235
  • Peer Reviewed / Open Access
• [Journal Article] Histone acetylation orchestrates wound-induced transcriptional activation and cellular reprogramming in Arabidopsis (2019)
  • Author(s): Rymen Bart、Kawamura Ayako、Lambolez Alice、Inagaki Soichi、Takebayashi Arika、Iwase Akira、Sakamoto Yuki、Sako Kaori、Favero David S.、Ikeuchi Momoko、Suzuki Takamasa、Seki Motoaki、Kakutani Tetsuji、Roudier Fran?ois、Sugimoto Keiko
  • Journal Title: Communications Biology Volume: 2 Pages: 404(15pages)
  • DOI: 10.1038/s42003-019-0646-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] NF-YB2 and NF-YB3 Have Functionally Diverged and Differentially Induce Drought and Heat Stress-Specific Genes (2019)
  • Author(s): Sato Hikaru、Suzuki Takamasa、Takahashi Fuminori、Shinozaki Kazuo、Yamaguchi-Shinozaki Kazuko
  • Journal Title: Plant Physiology Volume: 180 Pages: 1677～1690
  • DOI: 10.1104/pp.19.00391
  • Peer Reviewed / Open Access
• [Journal Article] LSD1-LIKE1-Mediated H3K4me2 Demethylation Is Required for Homologous Recombination Repair (2019)
  • Author(s): Hirakawa Takeshi、Kuwata Keiko、Gallego Maria E.、White Charles I.、Nomoto Mika、Tada Yasuomi、Matsunaga Sachihiro
  • Journal Title: Plant Physiology Volume: 181 Pages: 499～509
  • DOI: 10.1104/pp.19.00530
  • Peer Reviewed / Open Access
• [Journal Article] The formation of perinucleolar bodies is important for normal leaf development and requires the zinc‐finger DNA‐binding motif in Arabidopsis ASYMMETRIC LEAVES2 (2019)
  • Author(s): Luo Lilan、Ando Sayuri、Sakamoto Yuki、Suzuki Takanori、Takahashi Hiro、Ishibashi Nanako、Kojima Shoko、Kurihara Daisuke、Higashiyama Tetsuya、Yamamoto Kotaro T.、Matsunaga Sachihiro、Machida Chiyoko、Sasabe Michiko、Machida Yasunori
  • Journal Title: The Plant Journal Volume: 101 Pages: 1118～1134
  • DOI: 10.1111/tpj.14579
  • Peer Reviewed / Open Access
• [Journal Article] The Progression of Xylem Vessel Cell Differentiation is Dependent on the Activity Level of VND7 in Arabidopsis thaliana (2019)
  • Author(s): Hirai Risaku、Higaki Takumi、Takenaka Yuto、Sakamoto Yuki、Hasegawa Junko、Matsunaga Sachihiro、Demura Taku、Ohtani Misato
  • Journal Title: Plants Volume: 9 Pages: 39～39
  • DOI: 10.3390/plants9010039
  • Peer Reviewed / Open Access
• [Journal Article] Cyanidioschyzon merolae aurora kinase phosphorylates evolutionarily conserved sites on its target to regulate mitochondrial division (2019)
  • Author(s): Kato Shoichi、Okamura Erika、Matsunaga Tomoko M.、Nakayama Minami、Kawanishi Yuki、Ichinose Takako、Iwane Atsuko H.、Sakamoto Takuya、Imoto Yuuta、Ohnuma Mio、Nomura Yuko、Nakagami Hirofumi、Kuroiwa Haruko、Kuroiwa Tsuneyoshi、Matsunaga Sachihiro
  • Journal Title: Communications Biology Volume: 2 Pages: 477(9pages)
  • DOI: 10.1038/s42003-019-0714-x
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of DNA Repair Foci in Root Cells of Arabidopsis in Response to DNA Damage (2019)
  • Author(s): Hirakawa Takeshi、Matsunaga Sachihiro
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 990(9pages)
  • DOI: 10.3389/fpls.2019.00990
  • Peer Reviewed / Open Access
• [Journal Article] The SMC5/6 Complex Subunit NSE4A Is Involved in DNA Damage Repair and Seed Development (2019)
  • Author(s): D?az Mariana、Pe?inkov? Petra、Nowicka Anna、Baroux C?lia、Sakamoto Takuya、Gandha Priscilla Yuliani、Je??bkov? Hana、Matsunaga Sachihiro、Grossniklaus Ueli、Pecinka Ales
  • Journal Title: The Plant Cell Volume: 31 Pages: 1579～1597
  • DOI: 10.1105/tpc.18.00043
  • Peer Reviewed / Open Access
• [Journal Article] Abnormal leaf development of rpt5a mutant under zinc deficiency reveals important role of DNA damage alleviation for normal leaf development (2019)
  • Author(s): Sotta Naoyuki、Sakamoto Takuya、Matsunaga Sachihiro、Fujiwara Toru
  • Journal Title: Scientific Reports Volume: 9 Pages: 9369(10pages)
  • DOI: 10.1038/s41598-019-44789-w
  • Peer Reviewed / Open Access
• [Journal Article] The 26S Proteasome Is Required for the Maintenance of Root Apical Meristem by Modulating Auxin and Cytokinin Responses Under High-Boron Stress (2019)
  • Author(s): Sakamoto Takuya、Sotta Naoyuki、Suzuki Takamasa、Fujiwara Toru、Matsunaga Sachihiro
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 590(13pages)
  • DOI: 10.3389/fpls.2019.00590
  • Peer Reviewed / Open Access
• [Journal Article] Plant condensin II is required for the correct spatial relationship between centromeres and rDNA arrays (2019)
  • Author(s): Sakamoto Takuya、Sugiyama Tomoya、Yamashita Tomoe、Matsunaga Sachihiro
  • Journal Title: Nucleus Volume: 10 Pages: 116～125
  • DOI: 10.1080/19491034.2019.1616507
  • Peer Reviewed / Open Access
• [Journal Article] Acetic Acid Treatment Enhances Drought Avoidance in Cassava (Manihot esculenta Crantz) (2019)
  • Author(s): Utsumi Yoshinori、Utsumi Chikako、Tanaka Maho、Ha Chien Van、Takahashi Satoshi、Matsui Akihiro、Matsunaga Tomoko M.、Matsunaga Sachihiro、Kanno Yuri、Seo Mitsunori、Okamoto Yoshie、Moriya Erika、Seki Motoaki
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 521(12pages)
  • DOI: 10.3389/fpls.2019.00521
  • Peer Reviewed / Open Access
• [Journal Article] Primed histone demethylation regulates shoot regenerative competency (2019)
  • Author(s): Ishihara Hiroya、Sugimoto Kaoru、Tarr Paul T.、Temman Haruka、Kadokura Satoshi、Inui Yayoi、Sakamoto Takuya、Sasaki Taku、Aida Mitsuhiro、Suzuki Takamasa、Inagaki Soichi、Morohashi Kengo、Seki Motoaki、Kakutani Tetsuji、Meyerowitz Elliot M.、Matsunaga Sachihiro
  • Journal Title: Nature Communications Volume: 10 Pages: 1786(15pages)
  • DOI: 10.1038/s41467-019-09386-5
  • Peer Reviewed / Open Access
• [Journal Article] Pyrenocine A induces monopolar spindle formation and suppresses proliferation of cancer cells (2019)
  • Author(s): Myobatake Yusuke、Kamisuki Shinji、Tsukuda Senko、Higashi Tsunehito、Chinen Takumi、Takemoto Kenji、Hachisuka Masami、Suzuki Yuka、Takei Maya、Tsurukawa Yukine、Maekawa Hiroaki、Takeuchi Toshifumi、Matsunaga Tomoko M.、Sahara Hiroeki、Usui Takeo、Matsunaga Sachihiro、Sugawara Fumio
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: 27 Pages: 115149～115149
  • DOI: 10.1016/j.bmc.2019.115149
  • Peer Reviewed / Open Access
• [Journal Article] A Positive Feedback Loop Comprising LHW?TMO5 and Local Auxin Biosynthesis Regulates Initial Vascular Development in Arabidopsis Roots (2019)
  • Author(s): Ohashi-Ito Kyoko、Iwamoto Kuninori、Nagashima Yoshinobu、Kojima Mikiko、Sakakibara Hitoshi、Fukuda Hiroo
  • Journal Title: Plant and Cell Physiology Volume: 60 Pages: 2684～2691
  • DOI: 10.1093/pcp/pcz156
  • Peer Reviewed / Open Access
• [Journal Article] A Versatile Method for Mounting <em>Arabidopsis</em> Leaves for Intravital Time-lapse Imaging (2019)
  • Author(s): Betsuyaku Shigeyuki、Nomura Nobuhiko、Fukuda Hiroo
  • Journal Title: Journal of Visualized Experiments Volume: 144 Pages: e59147
  • DOI: 10.3791/59147
  • Peer Reviewed / Open Access
• [Journal Article] Triazine Probes Target Ascorbate Peroxidases in Plants (2019)
  • Author(s): Morimoto Kyoko、Cole Kyle S.、Kourelis Jiorgos、Witt Collin H.、Brown Daniel、Krahn Daniel、Stegmann Monika、Kaschani Farnusch、Kaiser Markus、Burton Jonathan、Mohammed Shabaz、Yamaguchi-Shinozaki Kazuko、Weerapana Eranthie、van der Hoorn Renier A.L.
  • Journal Title: Plant Physiology Volume: 180 Pages: 1848～1859
  • DOI: 10.1104/pp.19.00481
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Deciphering the non-coding RNA-level response to arsenic stress in rice (Oryza sativa) (2019)
  • Author(s): Tang Zhonghai、Xu Min、Ito Hidetaka、Cai Jiahui、Ma Xiaoxia、Qin Jingping、Yu Dongliang、Meng Yijun
  • Journal Title: Plant Signaling & Behavior Volume: 14 Pages: 1629268～1629268
  • DOI: 10.1080/15592324.2019.1629268
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MepmiRDB: a medicinal plant microRNA database (2019)
  • Author(s): Yu Dongliang、Lu Jiangjie、Shao Weishan、Ma Xiaoxia、Xie Tian、Ito Hidetaka、Wang Tingzhang、Xu Min、Wang Huizhong、Meng Yijun
  • Journal Title: Database Volume: 2019 Pages: baz070
  • DOI: 10.1093/database/baz070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Revisiting the Basal Role of ABA ? Roles Outside of Stress (2019)
  • Author(s): Yoshida Takuya、Christmann Alexander、Yamaguchi-Shinozaki Kazuko、Grill Erwin、Fernie Alisdair R.
  • Journal Title: Trends in Plant Science Volume: 24 Pages: 625～635
  • DOI: 10.1016/j.tplants.2019.04.008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seasonal Stability and Dynamics of DNA Methylation in Plants in a Natural Environment (2019)
  • Author(s): Ito、Nishio、Tarutani、Emura、Honjo、Toyoda、Fujiyama、Kakutani、Kudoh
  • Journal Title: Genes Volume: 10 Pages: 544～544
  • DOI: 10.3390/genes10070544
  • Peer Reviewed / Open Access
• [Journal Article] Genome Sequence of Striga asiatica Provides Insight into the Evolution of Plant Parasitism (2019)
  • Author(s): Yoshida Satoko、Kim Seungill、Wafula Eric K. et al. Shirasu Ken
  • Journal Title: Current Biology Volume: 29 Pages: 3041～3052.e4
  • DOI: 10.1016/j.cub.2019.07.086
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Regulation of stomatal movements in response to environmental signals (2019)
  • Author(s): Toshinori Kinoshita
  • Organizer: Frontiers in plant environmental response research ‘local signaling, long-distance communication and memory for developmental plasticity’
  • Int'l Joint Research / Invited
• [Presentation] Gene priming for shoot regeneration (2019)
  • Author(s): Sachihiro Matsunaga
  • Organizer: Frontiers in plant environmental response research: local signaling, long-distance communication and memory for developmental plasticity
  • Int'l Joint Research / Invited
• [Presentation] Live imaging system to track post-translational modification dynamics in Arabidopsis thaliana (2019)
  • Author(s): Mio K. Shibuta, Megumi Matsuoka, Mayu Yoshikawa, Kazuki Kurita, Tamako Yamaoka, Takyuya Sakamoto, Hiroshi Kimura, Sachihiro Matsunaga
  • Organizer: Frontiers in plant environmental response research: local signaling, long-distance communication and memory for developmental plasticity
  • Int'l Joint Research
• [Presentation] Regulation of stomatal movements by nutrients via long-distance signaling (2019)
  • Author(s): Toshinori Kinoshita
  • Organizer: Nitrogen2019
  • Int'l Joint Research / Invited
• [Presentation] Regulation of light-induced stomatal opening and plasma membrane H+-ATPase (2019)
  • Author(s): Toshinori Kinoshita
  • Organizer: The 18th International Workshop on Plant Membrane Biology
  • Int'l Joint Research / Invited
• [Presentation] Long-distance peptide signaling involved in systemic regulation of nitrogen acquisition (2019)
  • Author(s): Matsubayashi Y.
  • Organizer: 23rd International Conference on Plant Growth Substances
  • Int'l Joint Research / Invited
• [Presentation] Long distance peptide signaling mediating nitrogen homeostasis (2019)
  • Author(s): Matsubayashi Y.
  • Organizer: Cold Spring Harbor Asia conference on Plant Cell and Development Biology
  • Int'l Joint Research / Invited
• [Presentation] Transcriptional control of stress-induced cellular reprogramming in plants (2019)
  • Author(s): Keiko Sugimoto
  • Organizer: Cold Spring Harbor Asia Meeting
  • Int'l Joint Research / Invited
• [Presentation] Epigenetic control of cellular reprogramming in plants (2019)
  • Author(s): Keiko Sugimoto
  • Organizer: International Conference on Arabidopsis Research
  • Int'l Joint Research / Invited
• [Presentation] How do parasitic plants perceive host plants? (2019)
  • Author(s): Ken Shirasu
  • Organizer: IS-MPMI XVIII
  • Int'l Joint Research / Invited
• [Remarks] 環境記憶統合webページ
  • URL: https://www.rs.tus.ac.jp/plantmemory/index.html
• [Funded Workshop] Frontiers in plant environmental response research: local signaling, long-distance communication and memory for developmental plasticity (2019)