生殖細胞のエピゲノムダイナミクスとその制御

2013 Fiscal Year Annual Research Report

• Project Area: Analyses and regulation of germline epigenome
• Project/Area Number: 25112001
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Research Institution: Kyoto University
• Principal Investigator: 篠原 隆司 京都大学, 医学(系)研究科(研究院), 教授 (30322770)
• Co-Investigator(Kenkyū-buntansha): 伊川 正人 大阪大学, 微生物病研究所, 教授 (20304066)
• Project Period (FY): 2013-06-28 – 2018-03-31
• Keywords: 発生・分化

Research Abstract

平成25年度は領域の発足と共に以下の活動を行った。
1) 領域研究の推進体制の構築：3つの研究計画グループからなる計画研究の方針を確認した。11月に第一回公開シンポジウム (班会議) を大阪大学微生物病研究所で二日に渡り開催し、領域研究の方向付けを確認した。領域研究者間の情報交換、研究リソースの共有などを通じて連携体制の構築を強化する。その際に論文、学会発表、特許公開などで開示されていない情報 (最新データやネガティブデータ) に関しても共有できるよう便宜を図った（次の広報の項目参照）。2) 研究の広報：広報担当係を決定し、領域ホームページを開設した。研究体制と計画班員のメンバーの陣容を発表すると共に、領域の目的とその成果を、領域内外の研究者、専門家へ広く周知させた。また、ここには班員外のメンバーもアクセスが可能となる領域研究者間の情報交換のための情報交換サイトも作成している。3) 研究リソース:遺伝子改変マウスの共有や作製支援、エピゲノム解析支援、顕微授精・クローン作製支援などの研究支援事業についての具体的な実施方法を協議し、その計画を決定した。各計画班員からの情報収集も終え、実際の支援の開始もスタートしたこともあり、支援グループには研究代表者から消耗品についての支援を行った。4) 公募研究の決定：平成26年2月28日に新学術領域研究専門委員会が行われた。この場で計画班員3名に加えて、外部委員が参加し、公募研究の方針について協議し決定を行った。5) 若手勉強会の開催：本年度は公開シンポジウムの前日より若手勉強会を開催した。口頭発表、ポスター発表を行い、最優秀者1名にはベストプレゼンター賞を授与した。次回の若手勉強会については、最優秀者が中心となって企画し、それを計画班員メンバー1-2名がサポートして行うこととなった。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

初年度に予定していたweb pageの開設、若手勉強会、公開シンポジウムを無事に終了した。これに加えて、支援班を利用した共同研究についてもスタートすることができた。班員の役割分担についても確認を行い、これまでのところ順調に研究を開始することができていると判断している。

Strategy for Future Research Activity

本年度は昨年度とは異なり、若手勉強会を合宿形式で行う予定である。各計画班メンバーに加えて、公募研究班についても若手研究者に発表してもらう。合宿の内容については、従来は外部講演者のプレゼンテーションを受け身に聞くのみであったが、今回からは若手自身の企画をより積極的に取り入れていくことになっている。本年度の公開シンポジウムについては、九州大学において予定しており、計画班員に加えて、公募班員にも全て口頭発表をしてもらい、より大きな規模で開催する予定である。

Research Products

• [Journal Article] Cell-cycle-dependent colonization of mouse spermatogonial stem cells after transplantation into seminiferous tubules. (2014)
  • Author(s): Ishii K, Kanatsu-Shinohara M, Shinohara T.
  • Journal Title: J Reprod Dev Volume: 60 Pages: 37-46
  • Peer Reviewed
• [Journal Article] Liver-Specific γ-Glutamyl Carboxylase-Deficient Mice Display Bleeding Diathesis and Short Life Span. (2014)
  • Author(s): Azuma K, Tsukui T, Ikeda K, Shiba S, Nakagawa K, Okano T, Urano T, Horie-Inoue K, Ouchi Y, Ikawa M, Inoue S.
  • Journal Title: PLoS One. Volume: 9 Pages: e88643
  • DOI: 10.1371/journal.pone.0088643.
  • Peer Reviewed
• [Journal Article] GPI-Anchored Protein Complex, LY6K/TEX101, Is Required for Sperm Migration into the Oviduct and Male Fertility in Mice. (2014)
  • Author(s): Fujihara Y, Okabe M, Ikawa M.
  • Journal Title: Biol Reprod. Volume: 90 Pages: 60
  • DOI: 10.1095/biolreprod.113.112888
  • Peer Reviewed
• [Journal Article] Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes. (2014)
  • Author(s): Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, Ikawa M.
  • Journal Title: Dev Growth Differ. Volume: 56 Pages: 122-9
  • DOI: 10.1111/dgd.12113.
  • Peer Reviewed
• [Journal Article] CYP26B1 promotes male germ cell differentiation by suppressing STRA8-dependent meiotic and STRA8-independent mitotic pathways. (2014)
  • Author(s): Saba R, Wu Q, Saga Y.
  • Journal Title: Dev Biol. Volume: 389 Pages: 173-81
  • DOI: 10.1016/j.ydbio.2014.02.013. [
  • Peer Reviewed
• [Journal Article] NANOS2 promotes male germ cell development independent of meiosis suppression. (2014)
  • Author(s): Saba R, Kato Y, Saga Y.
  • Journal Title: Dev Biol. Volume: 385 Pages: 32-40
  • DOI: 10.1016/j.ydbio.2013.10.018.
  • Peer Reviewed
• [Journal Article] RNA sequencing-based identification of aberrant imprinting in cloned mice. (2014)
  • Author(s): Okae H, Matoba S, Nagashima T, Mizutani E, Inoue K, Ogonuki N, Chiba H, Funayama R, Tanaka S, Yaegashi N, Nakayama K, Sasaki H, Ogura A, Arima T.
  • Journal Title: Hum Mol Genet Volume: 23 Pages: 992-1001
  • DOI: 10.1093/hmg/ddt495.
  • Peer Reviewed
• [Journal Article] Role of Retinoic Acid and Fibroblast Growth Factor 2 in Neural Differentiation from Cynomolgus Monkey (Macaca fascicularis) Embryonic Stem Cells. (2014)
  • Author(s): Hatori M, Shimozawa N, Yasmin L, Suemori H, Nakatsuji N, Ogura A, Yagami K, Sankai T.
  • Journal Title: Comp Med. Volume: 64 Pages: 140-7
  • Peer Reviewed
• [Journal Article] Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells. (2014)
  • Author(s): Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S.
  • Journal Title: Cell Stem Cell. Volume: 14 Pages: 217-27
  • DOI: 10.1016/j.stem.2013.12.015.
  • Peer Reviewed
• [Journal Article] A heterozygous mutation of GALNTL5 affects male infertility with impairment of sperm motility. (2014)
  • Author(s): Takasaki N, Tachibana K, Ogasawara S, Matsuzaki H, Hagiuda J, Ishikawa H, Mochida K, Inoue K, Ogonuki N, Ogura A, Noce T, Ito C, Toshimori K, Narimatsu H.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111 Pages: 1120-5
  • DOI: 10.1073/pnas.1310777111.
  • Peer Reviewed
• [Journal Article] Understanding the X chromosome inactivation cycle in mice: A comprehensive view provided by nuclear transfer. (2014)
  • Author(s): Oikawa M, Inoue K, Shiura H, Matoba S, Kamimura S, Hirose M, Mekada K, Yoshiki A, Tanaka S, Abe K, Ishino F, Ogura A.
  • Journal Title: Epigenetics. Volume: 9 Pages: 204-11
  • DOI: 10.4161/epi.26939.
  • Peer Reviewed
• [Journal Article] 受精後のエピジェネティック制御 (2014)
  • Author(s): 中村 肇伸
  • Journal Title: 細胞工学 Volume: 33 Pages: 409-413
• [Journal Article] マウスにおけるCRISPR/Cas9を用いた遺伝子改変 (2014)
  • Author(s): 藤原祥高, 伊川正人
  • Journal Title: 最強のステップUPシリーズ 今すぐ始めるゲノム編集 Volume: 実験医学別冊 Pages: 95-107
• [Journal Article] 受精メカニズム新論争 ドグマの再構築 (2014)
  • Author(s): 伊川正人
  • Journal Title: 細胞工学 Volume: 33 Pages: 362-365
• [Journal Article] 精子と透明帯の相互作用 (2014)
  • Author(s): 藤原祥高, 伊川正人
  • Journal Title: 細胞工学 Volume: 33 Pages: 380-385
• [Journal Article] 哺乳類受精のライブイメージング (2014)
  • Author(s): 佐藤裕公, 広橋教貴
  • Journal Title: 細胞工学 Volume: 33 Pages: 393-399
• [Journal Article] 論文の大半は再現不能!?：KO実験のススメ (2014)
  • Author(s): 岡部勝
  • Journal Title: 細胞工学 Volume: 33 Pages: 420-421
• [Journal Article] 「顕微授精でわかること、できること」 (2014)
  • Author(s): 小倉淳郎, 黒滝陽子
  • Journal Title: 細胞工学 Volume: 33 Pages: 400-405
• [Journal Article] 「減数分裂の発生制御とゲノム情報の継承」 (2014)
  • Author(s): 中馬新一郎
  • Journal Title: 実験医学 Volume: 32 Pages: 853-858
• [Journal Article] RNAi and overexpression of genes in ovarian somatic cells. (2014)
  • Author(s): Saito K.
  • Journal Title: Methods Mol Biol. Volume: 1093 Pages: 25-33
  • Peer Reviewed
• [Journal Article] Active DNA demethylation is required for complete imprint erasure in primordial germ cells. (2014)
  • Author(s): Kawasaki Y, Lee J, Matsuzawa A, Kohda T, Kaneko-Ishino T, Ishino F.
  • Journal Title: Sci Rep. Volume: 4 Pages: 3658
  • DOI: : 10.1038/srep03658.
  • Peer Reviewed
• [Journal Article] Effects of dppa3 on DNA methylation dynamics during primordial gram cell development in mice. (2013)
  • Author(s): Nakashima H,Kimura T,kaga Y,Nakatani T,Seki Y,Nakamura T,Nakano T.
  • Journal Title: Biol Reprod. Volume: 88 Pages: 125
  • DOI: 10.1095/biolreprod.112.105932.
  • Peer Reviewed
• [Journal Article] GPAT2,a mitochondrial outer member protein,piRNA biogenesis in rerstem cell. (2013)
  • Author(s): Shiromoto Y,Kuramochi-Miyagawa S,Daiba A,Chuma S,Katanaya A,Katumata A,Nishimura K,Ohtaka M,Nakanishi M,Nakamura T,Yoshinaga K,Asada N,Nakamura S,Yasunaga T,Kojoma-Kita K,Itou D, Kimura T,Nakano T.
  • Journal Title: RNA. Volume: 19 Pages: 803-10
  • DOI: 10.1261/rna.038521.113.
  • Peer Reviewed
• [Journal Article] ROS are required for mouse spermatogonial stem cell self-renewal. (2013)
  • Author(s): Morimoto H, Iwata K, Ogonuki N, Inoue K, Ogura A, Kanatsu-Shinohara M, Morimoto T, Yabe-Nishimura C, Shinohara T.
  • Journal Title: Cell Stem Cell Volume: 12 Pages: 774-86
  • DOI: 10.1016/j.stem.2013.04.001.
  • Peer Reviewed
• [Journal Article] Regulation of pluripotency in male germline stem cells by Dmrt1. (2013)
  • Author(s): Takashima S, Hirose M, Ogonuki N, Ebisuya M, Inoue K, Kanatsu-Shinohara M, Tanaka T, Nishida E, Ogura A, Shinohara T.
  • Journal Title: Gene Dev Volume: 27 Pages: 1949-58
  • DOI: 10.1101/gad.220194.113.
  • Peer Reviewed
• [Journal Article] Enrichment of mouse spermatogonial stem cells based on aldehyde dehydrogenase activity. (2013)
  • Author(s): Kanatsu-Shinohara M, Mori Y, Shinohara T.
  • Journal Title: Biol Reprod Volume: 89 Pages: 140
  • DOI: 10.1095/biolreprod.113.114629.
  • Peer Reviewed
• [Journal Article] Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. (2013)
  • Author(s): Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, Ikawa M.
  • Journal Title: Sci Rep. Volume: 3 Pages: 3355
  • DOI: 10.1038/srep03355.
  • Peer Reviewed
• [Journal Article] Targeted disruption of fad24, a regulator of adipogenesis, causes pre-implantation embryonic lethality due to the growth defect at the blastocyst stage. (2013)
  • Author(s): Ochiai N, Nishizuka M, Miyamoto T, Miyoshi I, Ikawa M, Osada S, Imagawa M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 438 Pages: 301-5
  • DOI: 10.1016/j.bbrc.2013.07.061.
  • Peer Reviewed
• [Journal Article] Establishment of mouse model of MYH9 disorders: heterozygous R702C mutation provokes macrothrombocytopenia with leukocyte inclusion bodies, renal glomerulosclerosis and hearing disability. Suzuki N, Kunishima (2013)
  • Author(s): Suzuki N, Kunishima S, Ikejiri M, Maruyama S, Sone M, Takagi A, Ikawa M, Okabe M, Kojima T, Saito H, Naoe T, Matsushita T.
  • Journal Title: PLoS One. Volume: 8 Pages: e71187
  • DOI: 10.1371/journal.pone.0071187.
  • Peer Reviewed
• [Journal Article] Macrophage MHC and T-cell receptors essential for rejection of allografted skin and lymphoma. (2013)
  • Author(s): Tashiro-Yamaji J, Maeda S, Ikawa M, Okabe M, Kubota T, Yoshida R.
  • Journal Title: Transplantation. Volume: 96 Pages: 251-7
  • DOI: 10.1097/TP.0b013e3182985527.
  • Peer Reviewed
• [Journal Article] Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. (2013)
  • Author(s): Inoue N, Hamada D, Kamikubo H, Hirata K, Kataoka M, Yamamoto M, Ikawa M, Okabe M and Hagihara Y.
  • Journal Title: Development. Volume: 140 Pages: 3221-9
  • DOI: 10.1242/dev.094854.
  • Peer Reviewed
• [Journal Article] MiR-200b and miR-429 Function in Mouse Ovulation and Are Essential for Female Fertility. (2013)
  • Author(s): Hasuwa H, Ueda J, Ikawa M, Okabe M.
  • Journal Title: Science. Volume: 341 Pages: 71-3
  • DOI: 10.1126/science.1237999.
  • Peer Reviewed
• [Journal Article] Recruitment of cyclin G2 to promyelocytic leukemia nuclear bodies promotes dephosphorylation of γH2AX following treatment with ionizing radiation. (2013)
  • Author(s): Naito Y, Yabuta N, Sato J, Ohno S, Sakata M, Kasama T, Ikawa M, Nojima H.
  • Journal Title: Cell Cycle. Volume: 12 Pages: 1773-84
  • DOI: 10.4161/cc.24878.
  • Peer Reviewed
• [Journal Article] Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa. (2013)
  • Author(s): Fujihara Y, Tokuhiro K, Muro Y, Kondoh G, Araki Y, Ikawa M, Okabe M.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110 Pages: 8111-6
  • DOI: 10.1073/pnas.1222166110.
  • Peer Reviewed
• [Journal Article] MEK/ERK signaling directly and indirectly contributes to the cyclical self-renewal of spermatogonial stem cells. (2013)
  • Author(s): Hasegawa K, Namekawa SH, Saga Y.
  • Journal Title: Stem Cells. Volume: 31 Pages: 2517-27
  • DOI: 10.1002/stem.1486.
  • Peer Reviewed
• [Journal Article] Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes. (2013)
  • Author(s): Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y, Kon Y.
  • Journal Title: Biol Reprod. Volume: 89 Pages: 3
  • DOI: 10.1095/biolreprod.113.107847.
  • Peer Reviewed
• [Journal Article] Somatic donor cell type correlates with embryonic, but not extra-embryonic, gene expression in postimplantation cloned embryos. (2013)
  • Author(s): Hirasawa R, Matoba S, Inoue K, Ogura A.
  • Journal Title: PLoS One. Volume: 8 Pages: e76422
  • DOI: 10.1371/journal.pone.0076422. eCollection 2013.
  • Peer Reviewed
• [Journal Article] Epigenetic regulation of mouse sex determination by the histone demethylase Jmjd1a. (2013)
  • Author(s): Kuroki S, Matoba S, Akiyoshi M, Matsumura Y, Miyachi H, Mise N, Abe K, Ogura A, Wilhelm D, Koopman P, Nozaki M, Kanai Y, Shinkai Y, Tachibana M.
  • Journal Title: Science. Volume: 341 Pages: 1106-9
  • DOI: 10.1126/science.1239864
  • Peer Reviewed
• [Journal Article] Naive-like conversion overcomes the limited differentiation capacity of induced pluripotent stem cells. (2013)
  • Author(s): Honda A, Hatori M, Hirose M, Honda C, Izu H, Inoue K, Hirasawa R, Matoba S, Togayachi S, Miyoshi H, Ogura A.
  • Journal Title: J Biol Chem. Volume: 288 Pages: 26157-66
  • DOI: 10.1074/jbc.M113.502492.
  • Peer Reviewed
• [Journal Article] The Arf GAP SMAP2 is necessary for organized vesicle budding from the trans-Golgi network and subsequent acrosome formation in spermiogenesis. (2013)
  • Author(s): Funaki T, Kon S, Tanabe K, Natsume W, Sato S, Shimizu T, Yoshida N, Wong WF, Ogura A, Ogawa T, Inoue K, Ogonuki N, Miki H, Mochida K, Endoh K, Yomogida K, Fukumoto M, Horai R, Iwakura Y, Ito C, Toshimori K, Watanabe T, Satake M.
  • Journal Title: Mol Biol Cell. Volume: 24 Pages: 2633-44
  • DOI: 10.1091/mbc.E13-05-0234.
  • Peer Reviewed
• [Journal Article] Mouse cloning using a drop of peripheral blood. (2013)
  • Author(s): Kamimura S, Inoue K, Ogonuki N, Hirose M, Oikawa M, Yo M, Ohara O, Miyoshi H, Ogura A.
  • Journal Title: Biol Reprod. Volume: 89 Pages: 24
  • DOI: 10.1095/biolreprod.113.110098.
  • Peer Reviewed
• [Journal Article] t-SNARE Syntaxin2 (STX2) is implicated in intracellular transport of sulfoglycolipids during meiotic prophase in mouse spermatogenesis. (2013)
  • Author(s): Fujiwara Y, Ogonuki N, Inoue K, Ogura A, Handel MA, Noguchi J, Kunieda T.
  • Journal Title: Biol Reprod. Volume: 88 Pages: 141
  • DOI: 10.1095/biolreprod.112.107110.
  • Peer Reviewed
• [Journal Article] Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse. (2013)
  • Author(s): Shiura H, Ikeda R, Lee J, Sato T, Ogonuki N, Hirose M, Ogura A, Ogawa T, Abe K.
  • Journal Title: Genesis. Volume: 51 Pages: 498-505
  • DOI: 10.1002/dvg.22391.
  • Peer Reviewed
• [Journal Article] RNAi-mediated knockdown of Xist does not rescue the impaired development of female cloned mouse embryos. (2013)
  • Author(s): Oikawa M, Matoba S, Inoue K, Kamimura S, Hirose M, Ogonuki N, Shiura H, Sugimoto M, Abe K, Ishino F, Ogura A.
  • Journal Title: J Reprod Dev. Volume: 59 Pages: 231-7
  • Peer Reviewed
• [Journal Article] Spermatogonial stem cell self-renewal and development. (2013)
  • Author(s): Kanatsu-Shinohara M, Shinohara T.
  • Journal Title: Annu Rev Cell Dev Biol Volume: 29 Pages: 163-87
  • DOI: 1146/annurev-cellbio-101512-122353.
  • Peer Reviewed
• [Journal Article] 「人工授精･体外受精･顕微授精」 繁殖生物学 (2013)
  • Author(s): 小倉淳郎
  • Journal Title: 日本繁殖生物学会編 Volume: インターズー Pages: 252-263
• [Journal Article] Clone-specific X-linked gene repression caused by ectopic Xist transcripts from the active X chromosome In: Principles of Cloning, 2nd Edition, pp. (2013)
  • Author(s): Ogura A, Inoue K.
  • Journal Title: Jose Cibelli et al., ed., Elsevier, Volume: 2nd Edition Pages: 161-172
• [Journal Article] 炎症と免疫 (2013)
  • Author(s): 小倉淳郎
  • Journal Title: 「医学用語解説 核移植」 Volume: 21 Pages: 466-468
• [Journal Article] Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice. (2013)
  • Author(s): Pandey RR, Tokuzawa Y, Yang Z, Hayashi E, Ichisaka T, Kajita S, Asano Y, Kunieda T, Sachidanandam R, Chuma S, Yamanaka S, Pillai RS.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110 Pages: 16492-7
  • DOI: 10.1073/pnas.1316316110.
  • Peer Reviewed
• [Journal Article] The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles. (2013)
  • Author(s): Lim AK, Lorthongpanich C, Chew TG, Tan CW, Shue YT, Balu S, Gounko N, Kuramochi-Miyagawa S, Matzuk MM, Chuma S, Messerschmidt DM, Solter D, Knowles BB.
  • Journal Title: Development. Volume: 140 Pages: 3819-25
  • DOI: 10.1242/dev.099184.
  • Peer Reviewed
• [Journal Article] Identification of the KDM2/7 Histone Lysine Demethylase Subfamily Inhibitor and its Antiproliferative Activity. (2013)
  • Author(s): Suzuki, T., Ozasa, H., Itoh, Y., Zhan, P., Sawada, H., Mino, K., Walport, L., Ohkubo, R., Kawamura, A., Yonezawa, M., Tsukada, Y., Tumber, A., Nakagawa, H., Hasegawa, M., Sasaki, R., Mizukami, T., Schofield, C., Miyata, N
  • Journal Title: J. Med. Chem. Volume: 56 Pages: 7222-31
  • DOI: 10.1021/jm400624b
  • Peer Reviewed
• [Journal Article] The discovery of histone demethylases. (2013)
  • Author(s): Shi, Y., Tsukada, Y.
  • Journal Title: Cold Spring Harb. Perspect. Biol. Volume: なし Pages: 1
  • DOI: pii: a017947. 10.1101/cshperspect.a017947.
  • Peer Reviewed
• [Journal Article] ヒストンのメチル化と脱メチル化. (2013)
  • Author(s): 束田裕一
  • Journal Title: エピジェネティクス (化学同人, 東京), 67-89 (2013). Volume: なし Pages: 67-89
• [Journal Article] DmGTSF1 is necessary for Piwi-piRISC-mediated transcriptional transposon silencing in the Drosophila ovary. (2013)
  • Author(s): Ohtani H, Iwasaki YW, Shibuya A, Siomi H, Siomi MC, Saito K.
  • Journal Title: Genes Dev. Volume: 27 Pages: 1656-61
  • DOI: 10.1101/gad.221515.113.
  • Peer Reviewed
• [Journal Article] The epigenetic regulation of transposable elements by PIWI-interacting RNAs in Drosophila (2013)
  • Author(s): Saito K.
  • Journal Title: Genes Genet Syst. Volume: 88 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Successful serial recloning in the mouse over multiple generations. (2013)
  • Author(s): Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT, Kishigami S, Ishino F, Wakayama T.
  • Journal Title: Cell Stem Cell. Volume: 12 Pages: 293-7
  • DOI: 10.1016/j.stem.2013.01.005.
  • Peer Reviewed
• [Journal Article] Effects of embryonic manipulation and epigenetics (2013)
  • Author(s): Kohda T.
  • Journal Title: J Hum Genet. Volume: 58 Pages: 416-20
  • DOI: : 10.1038/jhg.2013.61. Epub 2013 Jun 6. Review
  • Peer Reviewed
• [Presentation] マウス初期胚で顕微授精によって誘導される遺伝子発現調節の変化 (2013)
  • Author(s): 幸田尚、高木清考、及川真実、越後貫成美、井上貴美子、金児-石野知子、小倉淳郎、石野史敏
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 20131203-20131206
• [Presentation] マウス卵子におけるpiRNA の生合成機構と機能の解析 (2013)
  • Author(s): 樺山由佳，藤英博，刀谷在美，櫻井隆順，渡部聡朗，相賀裕美子，中馬新一郎，宮川さとみ，仲野徹，佐々木裕之
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 20131203-20131205
• [Presentation] CRISPR/Cas9システムを用いたKRAB zinc finger タンパク質の変異ES 細胞の樹立と機能解析 (2013)
  • Author(s): 大石裕晃，鵜木元香，福田渓，前之原章司，伊川 正人，佐渡敬，佐々木裕之
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 20131203-20131205
• [Presentation] マウス新生仔期の精原幹細胞の分化におけるメチローム変動 (2013)
  • Author(s): 久保直樹，藤英博，白根健次郎，白川峰征，神里亮人，曾根秀利，佐藤康人，鶴崎美徳，富澤信一，柴田弘紀，才津浩智，松本直通，大保和之，佐々木裕之
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 20131203-20131205
• [Presentation] Mouse oocyte methylomes at base resolution reveal genome-wide accumulation of non-CpG methylation and role of DNA methyltransferases (2013)
  • Author(s): Shirane, K., Toh, H, Kobayashi, H, Miura, F, Ito, T, Kono, T, and Sasaki, H.
  • Organizer: IHEC annual meeting 2013 & Science Days
  • Place of Presentation: Berlin, Germany
  • Year and Date: 20131110-20131112
• [Presentation] Regulation mechanism of DNA oxidation in mammalian zygote. (2013)
  • Author(s): Tsukada, Y. and Nakayama, K. I.
  • Organizer: 8th Annual Conference of Asia Epigenome Alliance and 2nd Taipei Epigenetics and Chromatin Meeting.
  • Place of Presentation: Taipei, Taiwan.
  • Year and Date: 20131106-20131108
  • Invited
• [Presentation] マウスSINE配列による近傍遺伝子制御 (2013)
  • Author(s): 一柳健司
  • Organizer: 2013年度国立遺伝学研究所研究集会「転移因子と宿主の相互作用による生命機能」
  • Place of Presentation: 静岡県三島市
  • Year and Date: 20131010-20131011
• [Presentation] マウス雄性生殖細胞でのレトロトランスポゾン制御におけるDNAメチル化とpiRNAの役割 (2013)
  • Author(s): 井上晃太、福田渓、一柳健司、佐々木裕之
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 20130919-20130921
• [Presentation] マウスB2 SINEのDNAメチル化制御機構とクロマチンバウンダリー機能 (2013)
  • Author(s): 一柳健司、一柳朋子、平福啓一伍、井上晃太、福田渓、佐々木裕之
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 20130919-20130921
• [Presentation] A unique regulatory phase of chromatin modification in the early mammalian embryo (2013)
  • Author(s): 束田裕一
  • Organizer: 第86回日本生化学会大会.
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 20130911-20130913
  • Invited
• [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明
  • Author(s): 中村肇伸
  • Organizer: 大阪大学蛋白質研究所セミナー「DNAメチル化の制御機構-メチル化模様形成、維持と消去」
  • Place of Presentation: 大阪府吹田市
  • Invited
• [Presentation] 精子を作る幹細胞のお話
  • Author(s): 篠原隆司
  • Organizer: 京大アカデミックデイ
  • Place of Presentation: 京都府京都市
• [Presentation] Question about the necessity of sperm binding to zona pellucida.
  • Author(s): 伊川正人
  • Organizer: SSR’s 47th annual meeting
  • Place of Presentation: Montreal, Canada
  • Invited
• [Presentation] CRISPR/Cas9システムを用いたマウスゲノム編集
  • Author(s): 伊川正人
  • Organizer: 第119回関西実験動物研究会
  • Place of Presentation: 大阪府吹田市
  • Invited
• [Presentation] CRISPR/Casシステムを用いたマウスゲノム編集
  • Author(s): 伊川正人
  • Organizer: 第２回動物実験科学シンポジウム
  • Place of Presentation: 愛知県名古屋市
  • Invited
• [Presentation] CRISPR/Casシステムを用いたマウスゲノム編集
  • Author(s): 伊川正人
  • Organizer: 第１０回生命資源研究・支援センターシンポジウム
  • Place of Presentation: 熊本県熊本市
  • Invited
• [Presentation] CRISPR/CAS mediated genome editing in mice
  • Author(s): 伊川正人
  • Organizer: International Symposium on RNAi and Genome Editing Research
  • Place of Presentation: 徳島県徳島市
  • Invited
• [Presentation] Maintenance of spermatogonial stem cell(SSCs) via Nanos2-mediated mechanism
  • Author(s): 相賀 裕美子, Zhou Zhi
  • Organizer: 第８６回日本生化学会
  • Place of Presentation: 神奈川県横浜市
  • Invited
• [Presentation] Germ cell specific factors, NANOS2 and NANOS3 interact with distinct components of the CNOT complex
  • Author(s): 相賀裕美子
  • Organizer: 第３６回日本分子生物学会
  • Place of Presentation: 兵庫県神戸市
  • Invited
• [Presentation] Epigenetic events in mammalian germ-cell development
  • Author(s): 佐々木裕之
  • Organizer: Gordon Research Conference on Germinal Stem Cell Biology
  • Place of Presentation: Hong Kong
  • Invited
• [Presentation] Genomic imprinting and the epigenome of mammalian germ cells
  • Author(s): 佐々木裕之
  • Organizer: 8th Annual Conference of Asia Epigenome Alliance, 2nd Taipei Epigenetics and Chromatin Meeting
  • Place of Presentation: Taipei, Taiwan
  • Invited
• [Presentation] DNA methylation profiles in mammalian germ cell development
  • Author(s): 佐々木裕之
  • Organizer: Annual Meeting & Science Days, IHEC 2013
  • Place of Presentation: Berlin, Germany
  • Invited
• [Presentation] 哺乳類生殖細胞のエピゲノム制御と小分子RNA
  • Author(s): 佐々木裕之
  • Organizer: 第25回高遠・分子細胞生物学シンポジウムin比叡山―生物学の新天地―
  • Place of Presentation: 京都府
  • Invited
• [Presentation] メチローム解析から見えた哺乳類の非CGメチル化
  • Author(s): 佐々木裕之
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 神奈川県横浜市
  • Invited
• [Presentation] ゲノムインプリンンティングの基礎と生殖医療
  • Author(s): 佐々木裕之
  • Organizer: 18回日本生殖内分泌学会
  • Place of Presentation: 東京都
  • Invited
• [Presentation] Analysis of the Dynamics of X Chromosome Inactivation by Nuclear Transfer
  • Author(s): 小倉淳郎
  • Organizer: The 61st NIBB Conference "Cellular Community in Mammalian Embryogenesis”
  • Place of Presentation: 愛知県岡崎市
  • Invited
• [Presentation] 体細胞核移植クローンの効率を決定する因子の探索
  • Author(s): 井上貴美子、小倉淳郎
  • Organizer: 第84回遺伝学会
  • Place of Presentation: 神奈川県横浜市
  • Invited
• [Presentation] 多能性幹細胞の大量/長期培養におけるゲノム情報の解析
  • Author(s): 中馬新一郎
  • Organizer: ＮＥＤＯ公開シンポジウム「再生医療の産業化を支える技術開発」 BioJapan2013
  • Place of Presentation: 神奈川県横浜市
• [Presentation] Mechanism of epigenome regulationin early embryo development.
  • Author(s): Tsukada, Y.
  • Organizer: The Fukuoka International Symposium on Genomics & Epigenomics 2013, –Expanding Frontiers of Genomic Science–.
  • Place of Presentation: 福岡県
  • Invited
• [Presentation] ショウジョウバエのpiRNAによるトランスポゾン制御
  • Author(s): 齋藤都暁
  • Organizer: 国立遺伝学研究所 研究集会「転移因子と宿主の相互作用による生命機能」
  • Place of Presentation: 静岡県
  • Invited
• [Presentation] piRNA-mediated epigenetic programming in Drosophila.
  • Author(s): Saito K
  • Organizer: International Symposium on RNAi and Genome Editing Methods.
  • Place of Presentation: 徳島県
  • Invited
• [Presentation] マウス初期胚のエピジェネティックな非対称性と遺伝子発現
  • Author(s): 幸田尚
  • Organizer: 大阪大学蛋白質研究所セミナーDNAメチル化の制御機構－メチル化模様形成、維持と消去－
  • Place of Presentation: 大阪府
  • Invited
• [Presentation] 「ゲノムインプリンティングの機構と個体発生」
  • Author(s): 幸田尚
  • Organizer: 農林水産省中央畜産技術研修会（畜産新技術Ａ・B）
  • Place of Presentation: 福島県
• [Presentation] Production of cloned mice from a drop of peripheral blood
  • Author(s): Kamimura S, Inoue K, Ogonuki N, Hirose M, Oikawa M, Yo M, Ohara O, Miyoshi H, Ogura A
  • Organizer: The 10th Annual Conference of the Asian Reproductive Biotechnology Society
  • Place of Presentation: Ho Chi Minh, Vietnam
• [Presentation] Nuclear transfer provides a new picture of the X chromosome inactivation cycle
  • Author(s): Oikawa M, Inoue K, Shiura H, Matoba S, Tanaka S, Abe K, Ishino F, Ogura A
  • Organizer: 26th Mouse Molecular Genetics
  • Place of Presentation: Cambridge, UK
• [Presentation] 生殖工学技術の改良による野生由来マウスの系統保存
  • Author(s): 持田慶司，長谷川歩未，伊集院麻衣子，田熊究一，高橋仁美，阪口真美子，橋本美知子，平岩典子，目加田和之，吉木淳，小倉淳郎
  • Organizer: 日本実験動物技術者協会第47回総会
  • Place of Presentation: 岡山県倉敷市
• [Presentation] 野生由来マウス系統における抗インヒビン血清投与による過排卵に適した週齢の検討
  • Author(s): 持田慶司，長谷川歩未，渡辺 元，田谷一善，小倉淳郎
  • Organizer: 第106回日本繁殖生物学会総会
  • Place of Presentation: 東京都府中市
• [Presentation] 卵子由来のXistのインプリントは着床期マウス胚から完全に消失する
  • Author(s): 及川真実，井上貴美子，的場章悟，志浦寛相，上村悟氏，越後貫成美，廣瀬美智子，田中智，阿部訓也，石野史敏，小倉淳郎
  • Organizer: 第106回日本繁殖生物学会総会
  • Place of Presentation: 東京都府中市
• [Presentation] The role of maternal GSE protein in DNA demethylation during zygote development in mice
  • Author(s): 畑中勇輝，清水なつみ，守田昂太郎，樋口智香，岸上哲士，小倉淳郎，松本和也
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] 最少精子数を用いた微小環境マウス体外受精法の確立
  • Author(s): 長谷川歩未，持田慶司，小倉淳郎
  • Organizer: 第106回日本繁殖生物学会総会
  • Place of Presentation: 東京都府中市
• [Presentation] 少ない精子数でも受精可能なmicroIVF(微小環境体外受精)法の確立に向けて
  • Author(s): 長谷川歩未，持田慶司，小倉淳郎
  • Organizer: 日本実験動物技術者協会第47回総会
  • Place of Presentation: 岡山県倉敷市
• [Presentation] マウスクローン胚におけるドナー細胞特異的遺伝子発現異常は着床後の胚体外組織において改善される
  • Author(s): 平澤竜太郎，的場章悟，井上貴美子，小倉淳郎
  • Organizer: 第106回日本繁殖生物学会総会
  • Place of Presentation: 東京都府中市
• [Book] エピジェネティクスキーワード事典 (2013)
  • Author(s): 牛島俊和, 真貝洋一編、鵜木元香,佐々木裕之
  • Total Pages: 315
  • Publisher: 羊土社
• [Book] 哺乳類の発生工学 (2013)
  • Author(s): 佐藤英明、河野友宏、内藤邦彦、小倉淳郎編
  • Total Pages: 200
  • Publisher: 朝倉書店