Elucidation of mechanisms that control chromatin movement in response to environmental stimuli

2019 Fiscal Year Annual Research Report

• Project Area: Integrative system of autonomous environmental signal recognition and memorization for plant plasticity
• Project/Area Number: 15H05962
• Research Institution: Tokyo University of Science
• Principal Investigator: 松永 幸大 東京理科大学, 理工学部応用生物科学科, 教授 (40323448)
• Project Period (FY): 2015-06-29 – 2020-03-31
• Keywords: クロマチン / エピジェネティクス / 再生 / 細胞核

Outline of Annual Research Achievements

環境刺激を受けるとクロマチンにエピジェネティックな変化が引き起こされる。その変化を制御する分子メカニズムを、植物のDNA損傷ストレス感受性を利用することで、ヒストン修飾やクロマチンリモデリングに関与する植物のクロマチンの動態制御因子を同定し、その機能を明らかにした。
1）クロマチンの核内配置を制御メカニズム
シロイヌナズナの細胞核では、ヘテロクロマチンであるセントロメア・クロマチンが核膜周縁部に分散配置する。この分散配置に関与している複合体を同定した。一方、核質側ではコンデンシンを介してセントロメア・クロマチンと相互作用する。各因子の変異体のHi-C解析を進めて、この複合体が全ゲノムレベルでDNA間の相互作用に影響を与えることがわかった。この複合体の変異体はDNA損傷に高感受性となることから、植物のクロマチン配置は環境刺激に対する応答メカニズムに関与していることが示唆された。
2）植物の器官再生におけるエピジェネティック・プライミング
エピジェネティック・プライミングは、将来の再生や分化に備えた待機状態において、エピジェネティクス変化やRNA polIIのpoisingなどが起こる現象である。シュートが再生されないヒストン脱メチル化酵素の変異体ldl3を同定し、ChIP-seqとRNA-seqの統合解析の結果、LDL3はヒストンH3K4のメリル残基を脱メチル化することがわかった。さらに、LDL3は細胞記憶の消去に関与しておらず、シュートの再生に必要な遺伝子群のH3K4をあらかじめ脱メチル化していた。つまり、シュート遺伝子群の発現をエピジェネティック・プライミングにより発現スタンバイ状態にしている仕組みが植物にあることがわかった。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] チューリッヒ大学(スイス)
  • Country Name: SWITZERLAND
  • Counterpart Institution: チューリッヒ大学
• [Int'l Joint Research] クレモント大学(フランス)
  • Country Name: FRANCE
  • Counterpart Institution: クレモント大学
• [Int'l Joint Research] 植物実験研究所(チェコ)
  • Country Name: CZECH
  • Counterpart Institution: 植物実験研究所
• [Int'l Joint Research] カーネギー研究所/カルフォルニア工科大学(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: カーネギー研究所/カルフォルニア工科大学
• [Journal Article] Deep imaging analysis in VISUAL reveals the role of YABBY genes in vascular stem cell fate determination. (2020)
  • Author(s): Nurani, A. M., Ozawa, Y., Furuya, T., Sakamoto, Y., Ebine, K., Matsunaga, S., Ueda, T., Fukuda, H., and Kondo, Y.
  • Journal Title: PLANT CELL PHYSIOL Volume: 61 Pages: 255-264
  • DOI: 10.1093/pcp/pcaa002
  • Peer Reviewed
• [Journal Article] The progression of xylem vessel cell differentiation is dependent on the activity level of VND7 in Arabidopsis thaliana. (2019)
  • Author(s): Hirai, R., Higaki, T., Takenaka, Y., Sakamoto, Y., Hasegawa, J., Matsunaga, S., Demura, T., and Ohtani, M.
  • Journal Title: Plants Volume: 9 Pages: -
  • DOI: 10.3390/plants9010039
  • Peer Reviewed
• [Journal Article] Pyrenocine A induces monopolar spindle formation and suppresses proliferation of cancer cells. (2019)
  • Author(s): Myobatake, Y., Kamisuki, S., Tsukuda, S., Higashi, T., Chinen, T., Takemoto, K., Hachisuka, M., Suzuki, Y., Takei, M., Tsurukawa, Y., Maekawa, H., Takeuchi, T., Matsunaga, T. M., Sahara, H., Usui, T., Matsunaga, S., and Sugawara, F.
  • Journal Title: Bioorg. Med. Chem. Volume: 27 Pages: 115149
  • DOI: 10.1016/j.bmc.2019.115149
  • Peer Reviewed
• [Journal Article] Lysine-specific demethylase epigenetically regulates human and plant phenomena. (2019)
  • Author(s): Toyoda, Y. and Matsunaga, S.
  • Journal Title: CYTOLOGIA Volume: 84 Pages: 295-298
  • DOI: 10.1508/cytologia.84.295
  • Peer Reviewed
• [Journal Article] Cyanidioschyzon merolae aurora kinase phosphorylates evolutionarily conserved sites on its target to regulate mitochondrial division. (2019)
  • Author(s): Kato, S., Okamura, E., Matsunaga, T. M., Nakayama, M., Kawanishi, Y., Ichinose, T., Iwane, A. H., Sakamoto, T., Imoto, Y., Ohnuma, M., Nomura, Y., Nakagami, H., Kuroiwa, H., Kuroiwa, T., and Matsunaga, S.
  • Journal Title: Commun. Biol. Volume: 2 Pages: 477
  • DOI: 10.1038/s42003-019-0714-x
  • Peer Reviewed / Open Access
• [Journal Article] LSD1-LIKE1-mediated H3K4me2 demethylation is required for homologous recombination repair. (2019)
  • Author(s): Hirakawa, T., Kuwata, K., Gallego, M. E., White, C. I., Nomoto, M., Tada, Y., and Matsunaga, S.
  • Journal Title: PLANT PHYSIOL Volume: 181 Pages: 499-509
  • DOI: 10.1104/pp.19.00530
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Intracellular localization of histone deacetylase HDA6 in plants. (2019)
  • Author(s): Kurita, K., Sakamoto, Y., Naruse, S., Matsunaga, T. M., Arata, H., Higashiyama, T., Habu, Y., Utsumi, Y., Utsumi, C., Tanaka, M., Takahashi, S., Kim, J. M., Seki, M., Sakamoto, T., and Matsunaga, S.
  • Journal Title: J PLANT RES Volume: 132 Pages: 629-640
  • DOI: 10.1007/s10265-019-01124-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Characterization of DNA repair foci in root cells of Arabidopsis in response to DNA damage. (2019)
  • Author(s): Hirakawa, T. and Matsunaga, S.
  • Journal Title: FRONT PLANT SCI Volume: 10 Pages: 990
  • DOI: 10.3389/fpls.2019.00990
  • Peer Reviewed / Open Access
• [Journal Article] The SMC5/6 Complex Subunit NSE4A Is Involved in DNA Damage Repair and Seed Development. (2019)
  • Author(s): Diaz, M., Pecinkova, P., Nowicka, A., Baroux, C., Sakamoto, T., Gandha, P. Y., Jerabkova, H., Matsunaga, S., Grossniklaus, U., and Pecinka, A.
  • Journal Title: PLANT CELL Volume: 31 Pages: 1579-1597
  • DOI: 10.1105/tpc.18.00043
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 2A peptides contribute to the co-expression of proteins for imaging and genome editing. (2019)
  • Author(s): Shibuta, M. K., Matsuoka, M. and Matsunaga S.
  • Journal Title: CYTOLOGIA Volume: 84 Pages: 107-111
  • DOI: 10.1508/cytologia.84.107
  • Peer Reviewed
• [Journal Article] Abnormal leaf development of rpt5a mutant under zinc deficiency reveals important role of DNA damage alleviation for normal leaf development. (2019)
  • Author(s): Sotta, N., Sakamoto, T., Matsunaga, S., and Fujiwara, T.
  • Journal Title: Scientific Reports Volume: 9 Pages: 9369
  • DOI: 10.1038/s41598-019-44789-w
  • Peer Reviewed / Open Access
• [Journal Article] Plant condensin II is required for the correct spatial relationship between centromeres and rDNA arrays. (2019)
  • Author(s): Sakamoto, T., Sugiyama, T., Yamashita, T., and Matsunaga, S.
  • Journal Title: NUCLEUS-PHILA Volume: 10 Pages: 116-125
  • DOI: 10.1080/19491034.2019.1616507
  • Peer Reviewed
• [Journal Article] The 26S proteasome is required for the maintenance of root apical meristem by modulating auxin and cytokinin responses under high-boron stress. (2019)
  • Author(s): Sakamoto, T., Sotta, N., Suzuki, T., Fujiwara, T., and Matsunaga, S.
  • Journal Title: FRONT PLANT SCI Volume: 10 Pages: 590-590
  • DOI: 10.3389/fpls.2019.00590
  • Peer Reviewed / Open Access
• [Journal Article] Primed histone demethylation regulates shoot regenerative competency. (2019)
  • Author(s): Ishihara, H., Sugimoto, K., Tarr, P. T., Temman, H., Kadokura, S., Inui, Y., Sakamoto, T., Sasaki, T., Aida, M., Suzuki, T., Inagaki, S., Morohashi, K., Seki, M., Kakutani, T., Meyerowitz, E. M., and Matsunaga, S.
  • Journal Title: NAT COMMUN Volume: 10 Pages: 1786
  • DOI: 10.1038/s41467-019-09386-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acetic acid treatment enhances drought avoidance in cassava (Manihot esculenta Crantz). (2019)
  • Author(s): Utsumi, Y., Utsumi, C., Tanaka, M., Ha, C. V., Takahashi, S., Matsui, A., Matsunaga, T. M., Matsunaga, S., Kanno, Y., Seo, M., Okamoto, Y., Moriya, E., and Seki, M.
  • Journal Title: FRONT PLANT SCI Volume: 10 Pages: 521
  • DOI: 10.3389/fpls.2019.00521
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1. (2019)
  • Author(s): Kobayashi, W., Liu, E., Ishii, H., Matsunaga, S., Schlogelhofer, P., and Kurumizaka, H.
  • Journal Title: JOURNAL OF BIOCHEMISTRY Volume: 165 Pages: 289-295
  • DOI: 10.1093/jb/mvy105
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Seasonal and diurnal regulation of flowering via an epigenetic mechanism in Arabidopsis thaliana. (2019)
  • Author(s): Shibuta, K. M. and Matsunaga, S.
  • Journal Title: CYTOLOGIA Volume: 84 Pages: 3-8
  • DOI: 10.1508/cytologia.84.3
  • Peer Reviewed
• [Journal Article] Heat and chilling stress induce nucleolus morphological changes. (2019)
  • Author(s): Hayashi, K. and Matsunaga, S.
  • Journal Title: JOURNAL OF PLANT RESEARCH Volume: 132 Pages: 395-403
  • DOI: 10.1007/s10265-019-01096-9
  • Peer Reviewed
• [Journal Article] 植物におけるエピジェネティクス状態の可視化技術 (2019)
  • Author(s): 栗田和貴、坂本卓也、松永幸大
  • Journal Title: アグリバイオ Volume: 3 Pages: 28-32
• [Journal Article] To regenerate or not to regenerate: factors that drive plant regeneration. (2019)
  • Author(s): Sugimoto, K., Temman, H., Kadokura, S., and Matsunaga, S.
  • Journal Title: CURRENT OPINION IN PLANT BIOLOGY Volume: 47 Pages: 138-150
  • DOI: 10.1016/j.pbi.2018.12.002
  • Peer Reviewed
• [Presentation] シロイヌナズナにおける新奇核膜内膜局在タンパク質の同定 (2019)
  • Author(s): 渡邉水音、坂本勇貴、坂本卓也、松永幸大
  • Organizer: 2019 年度 東京理科大学イメージングフロンティアセンターシンポジウム
• [Presentation] Live imaging system to tracking post-translational modification dynamics in Arabidopsis thaliana (2019)
  • Author(s): Mio K. Shibuta, Megumi Matsuoka, Mayu Yoshikawa, Kazuki Kurita, Tamako Yamaoka, Takuya Sakamoto, Hiroshi Kimura, Sachihiro Matsunaga
  • Organizer: IMPACT OF CHROMATIN DOMAINS ON PLANT PHENOTYPES
  • Int'l Joint Research
• [Presentation] FASCIATA1 is required for de-novo shoot regeneration in Arabidopsis thaliana (2019)
  • Author(s): Mariana Diaz、Takuya Sakamoto、 Sachihiro Matsunaga
  • Organizer: SEB conference on Imapct of chromatin domains on plant phenotypes
  • Int'l Joint Research
• [Presentation] Involvements of nuclear pore complex proteins in the regulation of spatial arrangement of chromatin domains in Arabidopsis (2019)
  • Author(s): Takuya Sakamoto、Yuka Oko、 Nanami Ito、Yuki Sakamoto、 Sachihiro Matsunaga
  • Organizer: SEB conference on Imapct of chromatin domains on plant phenotypes
  • Int'l Joint Research
• [Presentation] シロイヌナズナの間期セントロメア配置制御に核膜孔複合体が関与する (2019)
  • Author(s): 御子侑香、坂本卓也、伊藤ななみ、坂本勇貴、松永幸大
  • Organizer: 第4２回日本分子生物学会年会
• [Presentation] 植物における新奇核膜内膜局在タンパク質の同定 (2019)
  • Author(s): 渡邉水音、坂本勇貴、坂本卓也、松永幸大
  • Organizer: 第4２回日本分子生物学会年会
• [Presentation] Two-step regulation of centromere distribution in Arabidopsis thaliana (2019)
  • Author(s): Takuya Sakamoto, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: 4th International Symposium on Plant Nuclear Dynamics Supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Recent advances in nuclear dynamics in plants (2019)
  • Author(s): Sachihiro Matsunaga
  • Organizer: 4th International Symposium on Plant Nuclear Dynamics Supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Analysis of centromere distribution mechanism by nuclear pore complex (2019)
  • Author(s): Yuka Oko, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: 4th International Symposium on Plant Nuclear Dynamics Supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] FASCIATA1 is required for de-novo shoot regeneration in Arabidopsis thaliana (2019)
  • Author(s): Mariana Diaz and Sachihiro Matsunaga
  • Organizer: 4th International Symposium on Plant Nuclear Dynamics Supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Identification of novel proteins localized to the inner nuclear membrane in A. thaliana (2019)
  • Author(s): Minato Watanabe, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: 4th International Symposium on Plant Nuclear Dynamics Supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Analysis of an epigenetic regulator involved in tissue variation of regeneration competency in Arabidopsis thaliana (2019)
  • Author(s): Yuma Toyoda, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: 4th International Symposium on Plant Nuclear Dynamics Supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Identification of novel cohesin interaction factors (2019)
  • Author(s): Takayoshi Suzuki, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: 4th International Symposium on Plant Nuclear Dynamics Supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Live imaging system to track post-translational modifications dynamics in Arabidopsis thaliana (2019)
  • Author(s): Mio Shibuta and Sachihiro Matsunaga
  • Organizer: 4th International Symposium on Plant Nuclear Dynamics Supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Gene priming for shoot regeneration (2019)
  • Author(s): Sachihiro Matsunaga
  • Organizer: Frontiers in plant environmental response research: local signaling, long-distance communication and memory for developmental plasticity
  • Int'l Joint Research
• [Presentation] Live imaging system to track post-translational modification dynamics in Arabidopsis thaliana (2019)
  • Author(s): Mio K. Shibuta, Megumi Matsuoka, Mayu Yoshikawa, Kazuki Kurita, Tamako Yamaoka, Takyuya Sakamoto, Hiroshi Kimura, Sachihiro Matsunaga
  • Organizer: Frontiers in plant environmental response research: local signaling, long-distance communication and memory for developmental plasticity
  • Int'l Joint Research
• [Presentation] 藻類と培養細胞の融合 (2019)
  • Author(s): 青木遼太、福田牧葉、河西勇輝、松永幸大
  • Organizer: 第5回環境記憶統合・若手の会
• [Presentation] クロマチンドメインの配置制御機構における核膜孔複合体の機能解析 (2019)
  • Author(s): 伊藤ななみ、御子侑香、坂本卓也、松永幸大
  • Organizer: 第5回環境記憶統合・若手の会
• [Presentation] カルスを介した再生系における光受容体の影響の解析 (2019)
  • Author(s): 牧野亮介、杉本薫、坂本卓也、松永幸大
  • Organizer: 第5回環境記憶統合・若手の会
• [Presentation] 細胞融合によって動物細胞に植物染色体を移植する (2019)
  • Author(s): 河西勇輝、福田牧葉、青木遼太、松永幸大
  • Organizer: 第5回環境記憶統合・若手の会
• [Presentation] γ線照射によるシュート再生効率向上のメカニズム解析 (2019)
  • Author(s): 東海林朋佳、坂本勇貴、坂本卓也、松永幸大
  • Organizer: 第5回環境記憶統合・若手の会
• [Presentation] Mintbody enables to analyze intranuclear dynamics of phosphorylated RNA polymerase II (2019)
  • Author(s): 吉川真由, 山岡珠子, 澁田未央, 八木慎宜, 坂本卓也, 木村宏, 松永幸大
  • Organizer: 第5回環境記憶統合・若手の会
• [Presentation] カルスを介したシュート再生におけるE3 SUMOリガーゼNSE2の機能解析 (2019)
  • Author(s): 三浦理奈、Diaz Mariana、坂本卓也、松永幸大
  • Organizer: 第5回環境記憶統合・若手の会
• [Presentation] シロイヌナズナにおけるヒストン修飾H3K4me3のイメージング技術の確立 (2019)
  • Author(s): 松岡慈、坂本卓也、澁田未央、八木慎宜、松永幸大
  • Organizer: 第5回環境記憶統合・若手の会
• [Presentation] 核ラミナタンパク質 CRWN が環境応答遺伝子の発現を制御するメカニズムの解析 (2019)
  • Author(s): 坂本 勇貴，佐藤 繭子，豊岡 公徳，佐藤 良勝，高木 慎吾，松永 幸大
  • Organizer: 日本植物学会第83回大会
• [Presentation] DNA 損傷応答に与える光の影響の解析 (2019)
  • Author(s): 藤原 維，坂本 卓也，松永 幸大
  • Organizer: 日本植物学会第83回大会
• [Presentation] Cyanidioschyzon merolaeにおけるコヒーシンの染色体構造構築に関わる機能の解析 (2019)
  • Author(s): 中山 南，坂本 卓也，松永 朋子，北川 美也子，田中 寛，竹村 時空，鈴木 孝征，松永 幸大
  • Organizer: 日本植物学会第83回大会
• [Presentation] シロイヌナズナ 19S プロテアソーム変異体における塊根様組織形成を制御する因子の探索 (2019)
  • Author(s): 西岡 咲子，坂本 卓也，鈴木 孝征，諸橋 賢吾，安江 啓人，松永 幸大
  • Organizer: 日本植物学会第83回大会
• [Presentation] シロイヌナズナ茎頂組織における体細胞胚形成の解析 (2019)
  • Author(s): 伊藤 ななみ，角倉 慧，杉本 薫，鈴木 孝征，松永 幸大
  • Organizer: 日本植物学会第83回大会
• [Presentation] シロイヌナズナの新規染色体構造構築制御因子の解析 (2019)
  • Author(s): 坂本 卓也，坂本 勇貴，御子 侑香，伊藤 ななみ，松永 幸大
  • Organizer: 日本植物学会第83回大会
• [Presentation] 古倍数性アブラナ科植物Rorippa aquaticaの染色体レベルゲノムアセンブリ (2019)
  • Author(s): 坂本智昭、坂本卓也、松永幸大、木村成介
  • Organizer: 日本植物学会第83回大会
• [Presentation] 遺伝子プライミングによる再生メカニズム (2019)
  • Author(s): 松永 幸大，松岡 慈，澁田 未央，石原 弘也，天満 春花，角倉 慧，稲垣宗一，乾 弥生，鈴木 孝征，諸橋 賢吾，角谷 徹仁，杉本 薫，坂本 卓也
  • Organizer: 日本植物学会第83回大会
• [Presentation] 植物の根の DNA 損傷応答に与える光の影響の解析 (2019)
  • Author(s): 藤原維, 坂本卓也, 松永幸大
  • Organizer: 日本植物形態学会第31回大会
• [Presentation] 茎頂組織における体細胞胚形成の解析 (2019)
  • Author(s): 伊藤ななみ, 角倉慧, 杉本薫, 坂本卓也, 鈴木 孝征, 松永幸大
  • Organizer: 日本植物形態学会第31回大会
• [Presentation] Primed histone demethylation regulates shoot regenerative competency. (2019)
  • Author(s): 松永 幸大
  • Organizer: 日本植物形態学会第31回大会
• [Presentation] 植物核ラミナ CRWN は核内の遺伝子配置に関与する (2019)
  • Author(s): 坂本勇貴, 高木慎吾, 松永幸大
  • Organizer: 日本植物形態学会第31回大会
• [Presentation] シロイヌナズナにおける条件特異的な塊根様組織形成メカニズムの解析 (2019)
  • Author(s): 西岡咲子, 坂本卓也, 鈴木孝征, 安江啓人, 諸橋賢吾, 松永幸大
  • Organizer: 日本植物形態学会第31回大会
• [Presentation] シロイヌナズナの新規染色体構造構築制御因子の探索 (2019)
  • Author(s): 坂本卓也, 坂本勇貴, 御子侑香, 伊藤ななみ, 松永幸大
  • Organizer: 日本植物形態学会第31回大会
• [Presentation] Cyanidioschyzon merolae における染色体構造の形成に関与するコヒーシンの機能解析 (2019)
  • Author(s): 中山南, 坂本卓也, 松永朋子, 北川美也子, 竹村時空, 鈴木孝征, 田中寛, 松永幸大
  • Organizer: 日本植物形態学会第31回大会
• [Presentation] シロイヌナズナにおける翻訳後修飾ライブイメージングシステムの確立 (2019)
  • Author(s): 澁田未央、栗田和貴、松岡慈、坂本卓也、木村宏、松永幸大
  • Organizer: 日本遺伝学会第91回大会
• [Presentation] 植物DNA損傷応答におけるヒストン脱メチル化によるクロマチン構造制御 (2019)
  • Author(s): 平川 健、伊藤 寿朗、松永 幸大
  • Organizer: 日本遺伝学会第91回大会
• [Presentation] 原始紅藻Cyanidioschyzon merolaeにおける染色体構造の遺伝子発現制御への関与 (2019)
  • Author(s): 中山南、坂本卓也、松永朋子、北川美也子、田中寛、竹村時空、鈴木孝征、松永幸大
  • Organizer: アグリ・バイオ工学研究部門 公開シンポジウム
• [Presentation] シロイヌナズナ19Sプロテアソーム変異体における塊根様組織形成メカニズムの解析 (2019)
  • Author(s): 西岡咲子、坂本卓也、鈴木孝征、安江啓人、諸橋賢吾、松永幸大
  • Organizer: アグリ・バイオ工学研究部門 公開シンポジウム
• [Presentation] 植物のDNA損傷応答に与える光の影響の解析 (2019)
  • Author(s): 藤原維、坂本卓也、松永幸大
  • Organizer: アグリ・バイオ工学研究部門 公開シンポジウム
• [Presentation] 植物の再生系における細胞増殖と再生能の獲得は光合成と糖代謝を介してASHH2が制御している (2019)
  • Author(s): 牧野亮介、杉本薫、勝山雄喜、石原弘也、角倉慧、鈴木孝征、坂本卓也、松永幸大
  • Organizer: アグリ・バイオ工学研究部門 公開シンポジウム
• [Presentation] Live imaging system to tracking post-translational modification dynamics (2019)
  • Author(s): Mio Shibuta and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] ASHH2 regulates Arabidopsis leaf callus formation and shoot regeneration through photosynthesis/light and glucose metabolism pathways (2019)
  • Author(s): Ryosuke Makino, Yayoi Inui, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Two-step regulation of centromere distribution in Arabidopsis (2019)
  • Author(s): Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Establishment of live imaging tool mintbody in Arabidopsis thaliana (2019)
  • Author(s): Megumi Matsuoka, Mio Shibuta and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Analysis of centromere arranging mechanism by nuclear pore complex (2019)
  • Author(s): Yuka Oko, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Characterization of tuberous root-like tissues in Arabidopsis thaliana 19S proteasome mutants (2019)
  • Author(s): Sakiko Nishioka, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Comprehensive analysis of nuclear pore complex in centromere arrangement (2019)
  • Author(s): Nanami Ito, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Characterization of the chromosomal architecture in the primitive red alga Cyanidioschyzon merolae (2019)
  • Author(s): Minami Nakayama, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Recent research in plant nuclear dynamics (2019)
  • Author(s): Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Analysis of novel inner nuclear membrane proteins in plants (2019)
  • Author(s): Minato Watanabe, Yuki Sakamoto, Takuya Sakamoto and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] FASCIATA1 is required for de novo shoot regeneration in Arabidopsis thaliana (2019)
  • Author(s): Mariana Diaz and Sachihiro Matsunaga
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] Subnuclear gene positioning trough plant nuclear lamina (2019)
  • Author(s): Sachihiro Matsunaga and Yuki Sakamoto
  • Organizer: The 3rd International Symposium on Plant Nuclear Dynamics supported by JSPS and MEXT
  • Int'l Joint Research
• [Presentation] FASCIATA1 is required for de-novo shoot regeneration in Arabidopsis thaliana (2019)
  • Author(s): Mariana Diaz、Takuya Sakamoto、Kaoru Sugimoto、Sachihiro Matsunaga
  • Organizer: Plant Stem Cells: Surce of Plant Vitability
  • Int'l Joint Research
• [Presentation] Characterization of tuberous root-like organ formed in Arabidopsis thaliana (2019)
  • Author(s): Takuya Sakamoto、Sakiko Nishioka、Sachihiro Matsunaga
  • Organizer: Plant Stem Cells: Surce of Plant Vitability
  • Int'l Joint Research
• [Presentation] Roles of perinucleolar AS2 bodies and the nucleolus in the establishment of leaf polarity in Arabidopsis thaliana (2019)
  • Author(s): Sayuri Ando, Takumi Ogawa, Shoko Kojima, Yuki Sakamoto, Sachihiro Matsunaga, Yasunori Machida, and Chiyoko Machida
  • Organizer: Plant Organ Growth Symposium 2019
  • Int'l Joint Research
• [Presentation] Analysis of relationship between maintenance of heat stress memory and chromosome higher order structure (2019)
  • Author(s): Yui Fujiwara，Takuya Sakamoto，Yuki Sakamoto，Nobutoshi Yamaguchi，Toshiro Ito，Sachihiro Matsunaga
  • Organizer: Taiwan-Japan 2019 Plant Biology Conference
  • Int'l Joint Research
• [Presentation] Transcriptome analysis reveals dynamic expression changes during tuberous root formation in Arabidopsis thaliana (2019)
  • Author(s): Sakiko Nishioka、Takuya Sakamoto、Takamasa Suzuki、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Roles of nucleolar proteins for nuclear localization of zinc-finger-like protein ASYMMETRIC LEAVES2 (AS2) in leaf development of Arabidopsis thaliana (2019)
  • Author(s): Sayuri Ando、Takumi Ogawa、Shuichiro Goto、Shoko Kojima、Yuki Sakamoto、Sachihiro Matsunaga、Yasunori Machida、Chiyoko Machida
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Visualization of histone modification using live-imaging tool in plant (2019)
  • Author(s): Megumi Matsuoka、Takuya Sakamoto、Mio Shibuta、Noriyoshi Yagi、Hiroshi Kimura、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Spatial regulation involved in bi-directional differentiation of vascular cells in Arabidopsis (2019)
  • Author(s): Alif Meem Nurani、Yuki Kondo、Yuki Sakamoto、Kazuo Ebine、Sachihiro Matsunaga、Takashi Ueda、Hiroo Fukuda
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Florigen in cactus (2019)
  • Author(s): Natsuki Hasegawa、Keisuke Tanaka、Hirokazu Takahashi、Tomoaki Nishiyama、Yuki Sakamoto、Dario Copeti、Hisato Kobayashi、Mikio Nakazono、Kentaro K. Shimizu、Sachihiro Matsunaga、Hiroyuki Tsuji
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] ASHH2 Regulates Arabidopsis callus formation and shoot regeneration through photosynthesis/light and glucose metabolism pathways (2019)
  • Author(s): Ryosuke Makino、Kaoru Sugimoto、Yuki Katsuyama、Hiroya Ishihara、Satoshi Kadokura、Takamasa Suzuki、Takuya Sakamoto、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Histone demethylation is involved in gene priming for plant regeneration (2019)
  • Author(s): Sachihiro Matsunaga、Hiroya Ishihara、Haruka Tenman、Satoshi Kadokura、Soichi Inagaki、Yayoi Inui、Takuya Sakamoto、Takamasa Suzuki、Kengo Morohashi、Tetsuji Kakutani、Kaoru Sugimoto
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Chromatin remodeling factors are required for de-novo shoot regeneration in Arabidopsis thaliana (2019)
  • Author(s): Mariana Diaz、Kaoru Sugimoto、Takuya Sakamoto、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Analysis of relationship between maintenance of heat stress memory and chromosome higher order structure (2019)
  • Author(s): Yui Fujiwara、Takuya Sakamoto、Yuki Sakamoto、Nobutoshi Yamaguchi、Toshiro Ito、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Histone demethylase LDL1 promotes homologous recombination repair via demethylation of H3K4me2 (2019)
  • Author(s): Takeshi Hirakawa、Keiko Kuwata、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] シロイヌナズナの葉の発生分化に関わるZinc-finger-like タンパク質 AS2 の核局在における核小体タンパク質の役割 (2019)
  • Author(s): 安藤沙友里、小川拓海、後藤秀一朗、小島晶子、坂本勇貴、松永幸大、町田泰則、町田千代子
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Elucidation of chromosome structure and epigenetic mechanism of gene regulation in primitive red alga Cyanidioschyzon merolae (2019)
  • Author(s): Minami Nakayama、Takuya Sakamoto、Tomoko Matsunaga、Miyako Kitagawa、Kan Tanaka、Tokiaki Takemura、Takamasa Suzuki、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Nuclear lamina protein CRWN regulates gene positioning of a copper transport family gene locus (2019)
  • Author(s): Yuki Sakamoto、Mayuko Sato、Takamasa Suzuki、Kiminori Toyooka、Shingo Takagi、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Exploring biological meanings of the correct centromere distribution in plants (2019)
  • Author(s): Takuya Sakamoto、Yuki Sakamoto、Yuka Oko、Takamasa Suzuki、Stefan Grob、Ueli Grossniklaus、Sachihiro Matsunaga
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] ER stress-responsive transcription factors bZIP17 and bZIP28 regulate root elongation (2019)
  • Author(s): June-Sik Kim、Yuki Sakamoto、Fuminori Takahashi、Mikiko Kojima、Kaoru Urano、Hitoshi Sakakibara、Sachihiro Matsunaga、Kazuko Yamaguchi-Shinozaki、Kazuo Shinozaki
  • Organizer: 第60回日本植物生理学会年会
• [Remarks] 東京大学 松永研究室
  • URL: http://park.itc.u-tokyo.ac.jp/matsunaga_lab/
• [Remarks] 新学術領域・環境記憶
  • URL: https://www.rs.tus.ac.jp/plantmemory/
• [Funded Workshop] 3rd International Symposium on Nuclear Dynamics in Plants (2019)