Neo-lifestyle of fungal viruses

2020 Fiscal Year Annual Research Report

• Project Area: Neo-virology: the raison d'etre of viruses
• Project/Area Number: 16H06436
• Research Institution: Okayama University
• Principal Investigator: 鈴木 信弘 岡山大学, 資源植物科学研究所, 教授 (70206514)
• Co-Investigator(Kenkyū-buntansha): 近藤 秀樹 岡山大学, 資源植物科学研究所, 准教授 (40263628)
• Project Period (FY): 2016-06-30 – 2021-03-31
• Keywords: ネオライフスタイル / RNAウイルス / ヤドカリウイルス / ハダカウイルス / ヤドヌシウイルス / 裸性 / 宿主性／宿借性 / 菌類ウイルス

Outline of Annual Research Achievements

作物に感染し甚大な被害をもたらす糸状菌には、極めて多様なウイルスが高率に感染している。申請者は、既成のウイルス概念があてはまらない「裸性」・「宿借・宿主性」というユニークな生活環を持つ菌類ウイルスを果樹の病原糸状菌から発見した。本課題では、①ハダカウイルスの裸性（裸のRNAで感染組織で存在）、ヤドカリウイルス(YkV1)とヤドヌシウイルス(YnV1)の宿借・宿主性（ウイルス間の新しい相利共生関係）の証明とその分子基盤、②宿主菌生存圏の高次生態系（ウイルス・菌・植物）での前記ウイルスの役割（菌宿主の植物宿主での生育・生存適応能）の解明、さらに、③ネオ・ウイルスライフスタイル（裸性・宿借・宿主性）の普遍性を証明、を目指した。複数のFusarium属菌からハダカウイルス(HadV1)２系統を発見し、詳細に解析した。HadV1は10~11本の分節一本鎖RNAゲノムを持つこと、無性胞子と通じた垂直伝搬の効率は感染菌糸コロニーが古くなる程低下する、ゲノムRNA複製型dsRNAは共に感染菌糸破砕液中でRNAase感受性を示す（粒子とは異なり、裸状のRNA？）、HadV1は宿主菌に不顕性感染し平和的に共存していること、を明にした。YnV1にヘテロ粒子化されたYkV1が、自身のRNA合成酵素を利用して複製し、RNA合成酵素の活性化には2A様ペプチドを介したリボソームスキップ機構による翻訳が必須である、YnV1+YkV1はしゅくしゅきんの病原力衰退を惹起すること、を証明した。さらに、新規のヤドカリウイルス、ヤドヌシウイルスをスペイン産白紋羽病菌、バングラデシュ産糸状菌から、それぞれ発見した。これは、ヤドヌシ／ヤドカリウイルス間のパートナーシップ多様性の解明、ネオライフスタイルの普遍性の証明につながった。以上のように、ネオ・ライフスタイル（宿借・宿主性）の分子解剖・確立につながった。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix. (2021)
  • Author(s): Arjona-Lopez, J. M., Telengech, P., Suzuki, N., and Lopez-Herrera, C.
  • Journal Title: Fungal Biology Volume: 125 Pages: 69-76
  • DOI: 10.1016/j.funbio.2020.10.007
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range. (2021)
  • Author(s): Shahi, S., Chiba, S., Kondo, H., and Suzuki, N.
  • Journal Title: Virology Volume: 554 Pages: 55-65
  • DOI: 10.1016/j.virol.2020.11.011
  • Peer Reviewed
• [Journal Article] Biochemical characterization of the dicing activity of Dicer-like 2 in the model filamentous fungus Neurospora crassa. (2021)
  • Author(s): Tabara, M., Koiwa, H., Suzuki, N., and Fukuhara, T.
  • Journal Title: Fungal Genetics and Biology Volume: 146 Pages: 103488
  • DOI: 10.1016/j.fgb.2020.103488
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In-tree behavior of diverse viruses harbored in the chestnut blight fungus, Cryphonectria parasitica. (2021)
  • Author(s): Suzuki, N., Aulia, A., Shahi, S., Hillman, B. I., Cornerjo, C., Rigling, D.
  • Journal Title: Journal of Virology Volume: 95 Pages: e01962-20
  • DOI: 10.1128/JVI.01962-20
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of an RNA silencing suppressor encoded by a symptomless fungal hypovirus, Cryphonectria hypovirus 4. (2021)
  • Author(s): Aulia, A., Hyodo, K., Hisano, S., Kondo, H., I. B., Hillman, B. I., and Suzuki, N.
  • Journal Title: Biology (Basel) Volume: 10 Pages: 100
  • DOI: 10.3390/biology10020100
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pathogenetic roles of beet necrotic yellow vein virus RNA5 in the exacerbation of symptoms and yield reduction, development of scab-like symptoms, and Rz1-resistance breaking in sugar beet. (2021)
  • Author(s): Tamada, T., Uchino, H., Kusume, T., Iketani-Saito, M., Chiba, S., Andika, I. B., Kondo, H.
  • Journal Title: Plant Pathology Volume: 70 Pages: 219-232
  • DOI: 10.1111/ppa.13266
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] An introduction to fungal viruses. (2021)
  • Author(s): Suzuki, N.
  • Journal Title: Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). Volume: vol 4 Pages: 431-442
  • DOI: 10.1016/B978-0-12-814515-9.00045-X
  • Peer Reviewed
• [Journal Article] Yado-kari virus 1 and yado-nushi virus 1 (Unassigned). (2021)
  • Author(s): Das, S. and Suzuki, N.
  • Journal Title: Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). Volume: vol 4 Pages: 658-663
  • DOI: 10.1016/B978-0-12-814515-9.20949-1
  • Peer Reviewed
• [Journal Article] Quadriviruses (Quadriviridae). (2021)
  • Author(s): Kondo, H., Caston, J. R., and Suzuki, N.
  • Journal Title: Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). Volume: vol 4 Pages: 642-647
  • DOI: 10.1016/B978-0-12-814515-9.20948-X
  • Peer Reviewed
• [Journal Article] Megabirnaviruses (Megabirnaviridae). (2021)
  • Author(s): Sato, Y., and Suzuki, N.
  • Journal Title: Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). Volume: vol 4 Pages: 594-600
  • DOI: 10.1016/B978-0-12-814515-9.20947-8
  • Peer Reviewed
• [Journal Article] Structure of dsRNA Mycoviruses. (2021)
  • Author(s): Caston, J. R., Suzuki, N., and Ghabrial, S. A.
  • Journal Title: Encyclopedia of Virology 4th Edition (Ed. D. Bamford & M. Zuckerman). Volume: vol 4 Pages: 504-512
  • DOI: 10.1016/B978-0-12-809633-8.21275-2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Virome analysis of aphid populations that infest the barley field: the discovery of two novel groups of nege/kita-like viruses and other novel RNA viruses. (2020)
  • Author(s): Kondo, H., Fujita, M., Hisano, H., Hyodo, K., Andika I. B., and Suzuki, N.
  • Journal Title: Frontiers in Microbiology Volume: 11 Pages: 509
  • DOI: 10.3389/fmicb.2020.00509
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diverse partitiviruses from the phytopathogenic fungus, Rosellinia necatrix. (2020)
  • Author(s): Telengech, P., Hisano, S., Micheni, C. M., Hyodo, K., Arjona-Lopez, J.M., Lopez-Herrera, C., Kanematsu, S., Kondo, H., and Suzuki, N.
  • Journal Title: Frontiers in Microbiology Volume: 11 Pages: 1064
  • DOI: 10.3389/fmicb.2020.01064
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hadaka virus 1: A capsidless 11-segmented (+)RNA virus from a phytopathogenic fungus Fusarium oxysporum. (2020)
  • Author(s): Sato, Y., Shamsi, W., Jamal, A., Bhatti, M. F., Kondo, H., and Suzuki, N.
  • Journal Title: mBIo Volume: 11 Pages: e0045-20
  • DOI: 10.1128/mBio.00450-20
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dicer monitoring in a model filamentous fungus host, Cryphonectria parasitica. (2020)
  • Author(s): Aulia, A., Tabara, M., Telengech, P., Fukuhara, T., and Suzuki, N.
  • Journal Title: Current Research in Virological Science Volume: 1 Pages: 100001
  • DOI: 10.1016/j.crviro.2020.100001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Coinfection of Rosellinia necatrix by a partitivirus and a virga-like virus is associated with hypovirulence. (2020)
  • Author(s): Arjona-Lopez, J. M., Telengech, P., Suzuki, N., and Lopez-Herrera, C.
  • Journal Title: European Journal of Plant Pathology Volume: 158 Pages: 111-119
  • DOI: 10.1007/s10658-020-02058-x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular characterization of a novel polymycovirus from Penicillium janthinellum with a focus on its genome-associated PASrp. (2020)
  • Author(s): Sato, Y., Jamal, A., Kondo, H., and Suzuki, N.
  • Journal Title: Frontiers in Microbiology Volume: 11 Pages: 592789
  • DOI: 10.3389/fmicb.2020.592789
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Establishment of Neurospora crassa as a model organism for fungal virology. (2020)
  • Author(s): Honda, S., Ana Eusebio-Cope, A., Miyashita, S., Yokoyama, A., Aulia, A., Shahi, S., Kondo, H., and Suzuki, N.
  • Journal Title: Nature Communications Volume: 11 Pages: 5627
  • DOI: 10.1038/s41467-020-19355-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structure and assembly of double-stranded RNA mycovirus. (2020)
  • Author(s): Mata, C. P., Rodriguez, J. M., Suzuki, N., and Caston, J. R.
  • Journal Title: Advances in Virus Research Volume: 108 Pages: 213-247
  • DOI: 10.1016/bs.aivir.2020.08.001
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Subcellular localization of carrot temperate virus 1 capsid protein in the nucleus and cytoplasm. (2021)
  • Author(s): Paul Telengech
  • Organizer: The Annual Meeting of the Japanese Phytopathological Society.
• [Presentation] Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range (2021)
  • Author(s): Sabitree Shahi
  • Organizer: The Annual Meeting of the Japanese Phytopathological Society.
• [Presentation] In-tree behavior of diverse viruses infecting the chestnut blight fungus. (2020)
  • Author(s): Nobuhiro Suzuki
  • Organizer: The 39th Annual Meeting of the American Society for Virology 2020
  • Int'l Joint Research
• [Presentation] Maintenance and loss of dispensable genomic segments of a polymyco-like capsidless fungal virus with an eleven-segmented RNA genome during its infection cycle. (2020)
  • Author(s): Yukiyo Sato
  • Organizer: The 39th Annual Meeting of the American Society for Virology 2020
  • Int'l Joint Research
• [Presentation] 2A-mediated co-translational cleavage is prerequisite for the viability of yado-kari virus 1 hosted by yado-nushi virus 1. (2020)
  • Author(s): Subha Das
  • Organizer: The 39th Annual Meeting of the American Society for Virology 2020
  • Int'l Joint Research
• [Presentation] Proof of concept for the neo-viral lifestyle of yado-kari virus 1 being hosted by yado-nushi virus 1 (2020)
  • Author(s): Subha Das
  • Organizer: Virology Meeting for Early Career Researchers 2020
• [Presentation] Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range (2020)
  • Author(s): Sabitree Shahi
  • Organizer: International Symposium on Environmental and Life Science.
• [Book] 「ヤドカリ」「ヤドヌシ」と呼ばれるウイルスの共生関係 ( ネオウイルス学 河岡義裕編） (2021)
  • Author(s): 鈴木信弘
  • Total Pages: 123-134
  • Publisher: 集英社
  • ISBN: 9784087211597