Repertoire analysis of neo-self-recognizing receptors

2020 Fiscal Year Final Research Report

• Project Area: Creation, function and structure of neo-self
• Project/Area Number: 16H06499
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Toyama
• Principal Investigator: Kishi Hiroyuki 富山大学, 学術研究部医学系, 教授 (60186210)
• Project Period (FY): 2016-06-30 – 2021-03-31
• Keywords: ネオ・セルフ / 抗原受容体 / レパトア / 抗原認識

Outline of Final Research Achievements

To comprehensively analyze the function of TCRs that are obtained by neo-self-reactive receptor repertoire analysis, we have developed cFIT method and TAP-J method. By using these methods, we have identified various TCRs reacting to self-antigen, tumor cells, or foreign antigen. We have also developed the easy protocol to prepare soluble MHC/peptide complex using animal cells, which un-require refolding of proteins. By using soluble MHC/peptide complex prepared by the method, we have prepared TCR-like antibodies that recognize MHC/peptide complex as TCR does. Furthermore, we have performed many collaborations with researchers in and outside of the innovative area.

Free Research Field

免疫学

Academic Significance and Societal Importance of the Research Achievements

単一細胞解析技術およびNGS解析技術の進歩により、TCRのレパトアの網羅的解析は比較的簡単に行えるようになってきたが、得られたTCRの機能を網羅的に解析する技術はこれまでなかった。本研究成果により、数百個のTCRの機能を解析することが可能になり、様々なサンプルからネオ・セルフ特異的TCRを同定することが可能になった。今後、自己免疫疾患の発症機序の解明や腫瘍の新規治療法の開発などに貢献すると期待される。