2020 Fiscal Year Final Research Report
Strategic Structural Study of the Protom Motive Force
| Project Area | New Photosynthesis : Reoptimization of the solar energy conversion system |
|---|
| Project/Area Number |
16H06560
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Biological Sciences
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
KURISU GENJI 大阪大学, 蛋白質研究所, 教授 (90294131)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
斉藤 圭亮 東京大学, 先端科学技術研究センター, 准教授 (20514516)
|
|---|
| Project Period (FY) |
2016-06-30 – 2021-03-31
|
| Keywords | 構造生物化学 / 生物物理学 / 生体エネルギー変換 / 光合成 |
|---|
| Outline of Final Research Achievements |
Focusing on the "Proton motive force" which has been a weak point of structural biology, we have been able to advance structural analysis including hydrogen atoms by combining theoretical chemical calculations with X-ray structural analysis, NMR, and cryo-electron microscopy. By using this approach, we were able to identify the structural basis for the photosynthetic function, and for some of them, we were able to find out the bottleneck of supramolecular complex responsible for the photosynthetic reaction. Specifically, the structural basis of the proton transport pathway of the photosystem II complex, the pH-dependent interaction of the cytochrome b6f complex with FNR, the molecular mechanism of the Fd-dependent quinone reduction of the NDH complex, and the regulatory system mediated by the strong reducing power of ferredoxin were clarified. Our collaborative research with <A01> and <A02> groups has also yielded certain results.
|
| Free Research Field |
構造生物学，生物物理学
|
| Academic Significance and Societal Importance of the Research Achievements |
生命が持つ階層構造に沿って生命秩序の形成を理解しようとした場合，低分子の認識機構についてある程度統一的に理解されているが，pHに依存する蛋白質間の相互作用を含むシステムの場合には統一的に理解されていなかった。本研究課題で掲げる『構造を基盤としたプロトン排出の戦略的分子設計』では，素反応がネットワークとして機能する光合成反応システムに対し，計算化学，Ｘ線結晶解析，NMR，クライオ電子顕微鏡とあらゆる構造解析手法を相補的に活用し，A01，A02の各班との共同研究を進めることで，光合成構造生物学を単純な分子素子の解析から，より高次な生体機能の理解へと遷移させる新しい研究の方向性を示すことができた。
|
