2020 Fiscal Year Final Research Report

Development of cell-free proteome technology for analysis of the signal transduction based on protein molecule interaction

Planned Research

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Project Area	Integrative understanding of biological signaling networks based on mathematical science
Project/Area Number	16H06579
Research Category	Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Allocation Type	Single-year Grants
Review Section	Complex systems
Research Institution	Ehime University
Principal Investigator	SAWASAKI TATSUYA 愛媛大学, プロテオサイエンスセンター, 教授 (50314969)
Co-Investigator(Kenkyū-buntansha)	高橋宏隆愛媛大学, プロテオサイエンスセンター, 講師 (70432804)
Project Period (FY)	2016-06-30 – 2021-03-31
Keywords	プロテインアレイ / 無細胞 / サリドマイド / 炎症 / ジベレリン / 近位依存性ビオチン化酵素 / AirID
Outline of Final Research Achievements	We have created a 20,000-species human protein array (20K-HUPA) that covers almost all human proteins based on our originally developed wheat cell-free protein synthesis system, and have been studying (1) the development of large-scale analytical interaction analysis technology using 20K-HUPA, (2) the development of NF-κB signaling inhibitors, (3) the　(3) Elucidation and mathematical simulation of the response control mechanism of the plant hormone gibberellin, (4) Elucidation of the TGF-β signaling mechanism via SMAD3, which is ubiquitinated by MIB2, (5) Creation of a novel proximal-dependent biotinyltransferase, and (6) Construction of a proteolysis-inducing molecular analysis technology using thalidomide as a model. (5) creation of a novel proximal-dependent biotinyltransferase, and (6) construction of a molecular analysis technique to induce protein degradation using thalidomide as a model.
Free Research Field	蛋白質科学
Academic Significance and Societal Importance of the Research Achievements	ヒトのゲノムにコードされたタンパク質のほぼ全てを取り揃えることができたことから、薬剤と結合するタンパク質を同定することができ、その結果、副作用等の原因解明や副作用を軽減した薬剤の開発を進めることができる基盤技術を構築する事ができた。また、生体内のタンパク質は基本的に複合体を形成し機能している。本研究成果により、複合体を同定・解析できる技術ができたため、生命を支えるタンパク質を深く理解できる技術開発となった。