記憶・情動における多領野間脳情報動態の光学的計測と制御

2021 Fiscal Year Annual Research Report

• Project Area: Brain information dynamics underlying multi-area interconnectivity and parallel processing
• Project/Area Number: 17H06312
• Research Institution: The University of Tokyo
• Principal Investigator: 尾藤 晴彦 東京大学, 大学院医学系研究科(医学部), 教授 (00291964)
• Project Period (FY): 2017-06-30 – 2022-03-31
• Keywords: 神経科学 / 脳・神経

Outline of Annual Research Achievements

本計画では、以下の３点の研究項目に焦点を絞り、計画研究を遂行した。項目１）活性化細胞集団と記憶痕跡を多領野で同時に標識する活性化回路標識操作法を実現し、Ca2+イメージングと組み合わせ、連合学習時における大脳皮質での学習遂行時の記憶痕跡ダイナミクスと記憶痕跡細胞の活動動態の間の関係についてデータを取得し、解析を進めた。項目２）恐怖記憶記銘時における興奮性・抑制性細胞の活動バランスを記録する目的で、記憶課題遂行中の覚醒マウス脳の大脳皮質深部におけるライブ多光子Ca2+イメージングを実現する手法を開発し、データを取得した。項目３）脳全体での記憶痕跡標識操作を実現する遺伝子改変マウスや、大脳皮質局所で記憶痕跡を標識操作するウィルスベクターを用い、恐怖条件付け記憶における多領野間連関・並列処理を司る脳情報動態を光遺伝学的に再現し、情報表現にとって必要十分な成分を同定した。
引き続き検証実験を重ね、これらの実験を総合し、脳領野間の活動動態の必要性・十分性の検証を実現していく。

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Arc controls alcohol cue relapse by a central amygdala mechanism. (2023)
  • Author(s): Pagano R et al.
  • Journal Title: Mol Psychiatry Volume: 28 Pages: 733-745
  • DOI: 10.1038/s41380-022-01849-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Forster resonance energy transfer-based kinase mutation phenotyping reveals an aberrant facilitation of Ca2+/calmodulin-dependent CaMKIIα activity in de novo mutations related to intellectual disability. (2022)
  • Author(s): Fujii H, Kidokoro H, Kondo Y, Kawaguchi M, Horigane SI, Natsume J, Takemoto-Kimura S, Bito H.
  • Journal Title: Front Mol Neurosci. Volume: 15 Pages: 970031
  • DOI: 10.3389/fnmol.2022.970031
  • Peer Reviewed / Open Access
• [Journal Article] In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse using a Large Cranial Window. (2022)
  • Author(s): Manita S, Shigetomi E, Bito H, Koizumi S, Kitamura K.
  • Journal Title: J Vis Exp. Volume: 186 Pages: e64224
  • DOI: 10.3791/64224
  • Peer Reviewed / Open Access
• [Journal Article] A non-invasive system to monitor in vivo neural graft activity after spinal cord injury. (2022)
  • Author(s): Ago K, Nagoshi N, Imaizumi K, Kitagawa T, Kawai M, Kajikawa K, Shibata R, Kamata Y, Kojima K, Shinozaki M, Kondo T, Iwano S, Miyawaki A, Ohtsuka M, Bito H, Kobayashi K, Shibata S, Shindo T, Kohyama J, Matsumoto M, Nakamura M, Okano H.
  • Journal Title: Commun Biol. Volume: 5 Pages: 803
  • DOI: 10.1038/s42003-022-03736-8.
  • Peer Reviewed
• [Journal Article] Deciphering Ca2+-controlled biochemical computation governing neural circuit dynamics via multiplex imaging. (2022)
  • Author(s): Fujii H, Bito H.
  • Journal Title: Neurosci Res. Volume: 179 Pages: 79-90
  • DOI: 10.1016/j.neures.2022.04.004
  • Peer Reviewed
• [Journal Article] In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. (2022)
  • Author(s): Sakamoto M, Ota K, Kondo Y, Okamura M, Fujii H, Bito H.
  • Journal Title: STAR Protoc. Volume: 3 Pages: 101421
  • DOI: 10.1016/j.xpro.2022.101421
  • Peer Reviewed
• [Journal Article] A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. (2022)
  • Author(s): Sakamoto M, Inoue M, Takeuchi A, Kobari S, Yokoyama T, Horigane SI, Takemoto-Kimura S, Abe M, Sakimura K, Kano M, Kitamura K, Fujii H, Bito H.
  • Journal Title: Cell Rep Methods. Volume: 2 Pages: 100168
  • DOI: 10.1016/j.crmeth.2022.100168
  • Peer Reviewed / Open Access
• [Journal Article] Gastrin-releasing peptide regulates fear learning under stressed conditions via activation of the amygdalostriatal transition area. (2022)
  • Author(s): Goto F, Kiyama Y, Ogawa I, Okuno H, Ichise T, Ichise H, Anai M, Kodama T, Yoshida N, Bito H, Manabe T.
  • Journal Title: Mol Psychiatry Volume: 27 Pages: 1694-1703
  • DOI: 10.1038/s41380-021-01408-3
  • Peer Reviewed
• [Journal Article] Distinctive Regulation of Emotional Behaviors and Fear-Related Gene Expression Responses in Two Extended Amygdala Subnuclei With Similar Molecular Profiles. (2021)
  • Author(s): Ueda S, Hosokawa M, Arikawa K, Takahashi K, Fujiwara M, Kakita M, Fukada T, Koyama H, Horigane SI, Itoi K, Kakeyama M, Matsunaga H, Takeyama H, Bito H,
  • Journal Title: Front Mol Neurosci. Volume: 14 Pages: 741895
  • DOI: 10.3389/fnmol.2021.741895
  • Peer Reviewed / Open Access
• [Presentation] Multiplex imaging of neural activity and signaling dynamics (2022)
  • Author(s): Bito H.
  • Organizer: 6th International Symposium Frontiers in Neurophotonics
  • Int'l Joint Research / Invited
• [Presentation] A stepwise recruitment of cortical active ensembles into Arc-based engram cells during tone-reward associative memory formation (2022)
  • Author(s): Leonie S. Brebner, Michiko Okamura, Yayoi Kondo, Tatsushi Yokoyama, Ranmaru Shimoda, Ryang Kim, Hiroyuki Okuno, Haruhiko Bito
  • Organizer: Engrams and Neuronal Ensembles in Learning and Memory: from Molecules to Behaviour
  • Int'l Joint Research / Invited
• [Presentation] Deciphering brain information dynamics through probing of Ca2+ and Ca2+ signaling (2022)
  • Author(s): Bito H.
  • Organizer: Annual Meeting of the Japan Neuroscience Society
  • Invited
• [Presentation] Lessons learned from elucidating calcium-dependent neural circuit control mechanisms (2022)
  • Author(s): Bito H.
  • Organizer: 94th Annual Meeting of the Japanese Pharmacological Society
  • Invited