Interrogating cellular diversity by comprehensive tissue observation technique

2021 Fiscal Year Final Research Report

• Project Area: Integrated analysis and regulation of cellular diversity
• Project/Area Number: 17H06328
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Juntendo University (2021); The University of Tokyo (2017-2020)
• Principal Investigator: Etsuo Susaki 順天堂大学, 大学院医学研究科, 教授 (10444803)
• Project Period (FY): 2017-06-30 – 2022-03-31
• Keywords: 細胞、組織 / 組織透明化 / 組織3次元染色 / 3次元イメージング

Outline of Final Research Achievements

As a part of KAKEN-Shingakujutsu, this research specifically aimed to establish a comprehensive cell analysis technology named Cell-omics toward understanding of cellular diversity. During the research period, we invented 1) an advanced tissue clearing protocol by large-scale chemical screening, 2) world's best performance 3D tissue staining protocol, 3) a cloud software for whole brain single cell analysis. We also applied these developed technologies to various samples including human tissue or organoids. Therefore, we successfully established the cell-omics for cellular diversity research.

Free Research Field

システム生物学、バイオイメージング

Academic Significance and Societal Importance of the Research Achievements

本研究成果のセルオミクスは、多細胞システムの包括的な解析手段を提供する新規のフレームワークである。この解析手法は組織化学、神経科学、疾患生物学を含む、生体組織を対象とするあらゆる研究分野へのインパクトを与える。実際に、成果の一つである3次元組織学実現の論文（Susaki et al. Nat Commun. 2020）は、2年で3万件をこえる閲覧があり、掲載誌のLife science分野のTop50論文となるなど、多数の分野から高い注目を集めた。