Understanding of chemical communications and higher-order functional evaluation of bioactive ligands by spatiotemporal analysis

2021 Fiscal Year Final Research Report

• Project Area: Frontier research of chemical communications
• Project/Area Number: 17H06409
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Osaka University
• Principal Investigator: Kikuchi Kazuya 大阪大学, 工学研究科, 教授 (70292951)
• Project Period (FY): 2017-06-30 – 2022-03-31
• Keywords: 化学プローブ / in vivoイメージング / 破骨細胞 / DNAメチル化 / 蛋白質分解

Outline of Final Research Achievements

In this study, we developed chemical imaging probes for spatiotemporal analysis of the function of biomolecules and bioactive ligands in living cells and animals. We developed pH-responsive fluorescent probes that can be delivered to bone tissue for detecting osteoclast and osteocyte activity in living mice. We also evaluated the drug action on these cell activities. In addition, we developed small molecule-protein hybrid probes for visualization of important processes such as DNA methylation, endogenous membrane protein expression, and protein degradation in living cells.

Free Research Field

ケミカルバイオロジー

Academic Significance and Societal Importance of the Research Achievements

本研究で開発した化学プローブを利用したこれらの技術は生理活性リガンドが生きた細胞、個体においていつ、どこで対象分子や細胞に作用するのかを直接イメージング解析できる新たな評価法として期待できる。破骨細胞、骨細胞の活性法は骨粗鬆症や関節リウマチの発症に、またDNAメチル化や蛋白質分解はがんの病態と関連しているため、本評価法によってこれらの疾患の治療薬のスクリーニングや作用機序解析に貢献できることが考えられる。