2021 Fiscal Year Final Research Report
A chemical genomics approach for understanding chemical communication
| Project Area | Frontier research of chemical communications |
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| Project/Area Number |
17H06411
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Boone Charles 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (70601342)
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| Co-Investigator(Kenkyū-buntansha) |
八代田 陽子 国立研究開発法人理化学研究所, 環境資源科学研究センター, 副チームリーダー (60360658)
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| Project Period (FY) |
2017-06-30 – 2022-03-31
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| Keywords | chemical genomics / yeast / chemical communication / CRISPR |
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| Outline of Final Research Achievements |
We established a comprehensive chemical genomics (CG) analysis pipeline, employing various diagnostic sets of mutant budding yeast, Saccharomyces cerevisiae. We assembled a set of ~5,000 non-essential genes as viable haploid deletion mutants (HAP), and we generated several strain sets that span ~1,000 essential yeast genes, including a set of heterozygous diploid deletion mutants (HET), a set of temperature-sensitive (TS) mutants and a set of gene overexpression (MoBY) strains. We also established the fission yeast Schizosaccharomyces pombe version of CG pipeline with a set of ~2,200 non-essential genes (SpHAP). CG profiles of all strain collections often provide complementary evidence for target identification. We screened these HAP/HET/TS/MoBY strains against bioactive compounds. Moreover, we launched a human cell CG screening system. Thus, advancing our platform from yeast to human CG screening will yield high-quality molecules that are relevant for the development of drug leads.
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| Free Research Field |
Chemical genomics
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| Academic Significance and Societal Importance of the Research Achievements |
化合物の標的同定は創薬シーズ開発に重要であるばかりでなく、基礎研究に有用なケミカルツール分子の創成にも貢献する。我々が確立した、物理的相互作用によらず、化学遺伝学的相互作用をもとにした酵母ケミカルゲノミクス解析法は汎用性が広い。さらに本研究を発展させ、ヒト細胞のケミカルゲノミクス解析パイプラインにも着手できた。多種多様な生理活性物質の作用機序解明に有効な方法開発を達成したというインパクトは学術的にも社会的にも大きい。
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