2021 Fiscal Year Final Research Report
Clarification of the molecular mechanism of electron-transfer regulation in photosynthetic proteins by functional analyses
| Project Area | Creation of novel light energy conversion system through elucidation of the molecular mechanism of photosynthesis and its artificial design in terms of time and space |
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| Project/Area Number |
17H06435
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Complex systems
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
杉浦 美羽 愛媛大学, プロテオサイエンスセンター, 准教授 (80312255)
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| Project Period (FY) |
2017-06-30 – 2022-03-31
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| Keywords | 生物物理 / 光合成 / 赤外分光 / 水分解 / 電子移動 |
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| Outline of Final Research Achievements |
We studied the molecular mechanisms of photosynthetic water oxidation and electron-proton transfer in photosystem II (PSII), using spectroscopic analyses, mainly infrared spectroscopy, in combination with site-directed mutagenesis of cyanobacteria. As for the water oxidation mechanism, we obtained important information about the processes of proton and water transfer and the pathways in the proteins. In addition, we clarified the mechanism of photo-assembly of the Mn4CaO5 cluster, the catalytic site of water oxidation in PSII. Furthermore, we clarified the regulatory mechanism of the redox potentials of the iron-quinone electron acceptor, the charge distribution and the mechanism of the primary charge separation of the reaction center chlorophylls, and the role of the axial ligands of Cytb559 in the secondary electron transfer related to the photoprotection of PSII.
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| Free Research Field |
生物物理学
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| Academic Significance and Societal Importance of the Research Achievements |
光化学系Ⅱにおける電子・プロトン移動反応および水分解反応は、酸素発生型光合成の電子伝達鎖において鍵となる過程であり、その分子機構を明らかにしたことは、天然光合成における光エネルギー変換機構の全解明への極めて重要なステップとなる。また、現在人類が直面するエネルギー問題、地球温暖化問題の解決のためには、高効率な人工光合成系の開発および実用化が急務であり、本研究で得られた天然光合成機構の知見は、人工光合成系の設計への基盤を与え、社会に大きく貢献するものである。
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