Studies of proliferation and pathogenesis of extracellular gram-negative bacterial pathogens

2010 Fiscal Year Final Research Report

• Project Area: Matrix of Infection Phenomena
• Project/Area Number: 18073003
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: SASAKAWA Chihiro The University of Tokyo, 医科学研究所, 教授 (70114494)
• Co-Investigator(Kenkyū-buntansha): IIDA Tetsuya 大阪大学, 微生物病研究所, 特任教授 (90221746)
• Project Period (FY): 2006 – 2010
• Keywords: 赤痢菌 / 腸炎ビブリオ / III型分泌機構 / エフェクター / 感染 / 病原性

Research Abstract

(1) We have identified the interaction between Shigella effectors secreted via the type III secretion system (T3SS) and their targeting host factors, and elucidated their roles in promoting bacterial infection of intestinal epithelium. Based on our results, we have proposed the bacterial infection strategy involving in promoting the initial stage of infection.
(2) We have identified the effector proteins secreted via T3SS of Vibrio parahaemolyticus, and revealed their intracellular functions. Also, the analysis of the regulation mechanism of the T3SS genes revealed a possibility of a novel therapeutic means against bacterial diarrhea.

Research Products

• [Journal Article] BabA-mediated adherence is a potentiator of the Helicobacter pylori Type IV secretion system activity. (2011)
  • Author(s): Ishijima N, Suzuki M, Ashida H, Ichikawa Y, Kanegae Y, Saito I, Boren T, Haas R, Sasakawa C, Mimuro H.
  • Journal Title: J Biol Chem. in press
  • Peer Reviewed
• [Journal Article] A tecpr1-dependent selective autophagy pathway targets bacterial pathogens. (2011)
  • Author(s): Ogawa M, Yoshikawa Y, Kobayashi T, Mimuro H, Fukumatsu M, Kiga K, Piao Z, Ashida H, Yoshida M, Kakuta S, Koyama T, Goto Y, Nagatake T, Nagai S, Kiyono H, Kawalec M, Reichhart JM, Sasakawa C.
  • Journal Title: Cell Host Microbe. 9 Pages: 376-389
  • Peer Reviewed
• [Journal Article] Shigella deploy multiple countermeasures against host innate immune responses. (2011)
  • Author(s): Ashida H, Ogawa M, Kim M, Suzuki S, Sanada T, Punginelli C, Mimuro H, Sasakawa C.
  • Journal Title: Curr Opin Microbiol. 14 Pages: 16-23
  • Peer Reviewed
• [Journal Article] Bacterial interactions with the host epithelium. (2010)
  • Author(s): Kim M, Ashida H, Ogawa M, Yoshikawa Y, Mimuro H, Sasakawa C.
  • Journal Title: Cell Host Microbe. 8 Pages: 20-35
  • Peer Reviewed
• [Journal Article] A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. (2010)
  • Author(s): Ashida H, Kim M, Schmidt-Supprian M, Ma A, Ogawa M, Sasakawa C.
  • Journal Title: Nat Cell Biol. 12 Pages: 66-73
  • Peer Reviewed
• [Journal Article] Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a therapeutic potential for bile acid-sequestering agents. (2010)
  • Author(s): Gotoh K, Kodama T, Hiyoshi H, Izutsu K, Park K.-S, Dryselius R, Akeda Y, Honda T, Iida T.
  • Journal Title: PLoS One 5 e13365
  • Peer Reviewed
• [Journal Article] Shigella infection of intestinal epithelium and circumvention of the host innate defense system. (2009)
  • Author(s): Ashida H, Ogawa M, Mimuro H, Sasakawa C.
  • Journal Title: Curr Top Microbiol Immunol. 337 Pages: 231-255
  • Peer Reviewed
• [Journal Article] Listeria monocytogenes ActA-mediated escape from autophagic recognition. (2009)
  • Author(s): Yoshikawa Y, Ogawa M, Hain T, Yoshida M, Fukumatsu M, Kim M, Mimuro H, Nakagawa I, Yanagawa T, Ishii T, Kakizuka A, Sztul E, Chakraborty T, Sasakawa C.
  • Journal Title: Nat Cell Biol. 11 Pages: 1233-1240
  • Peer Reviewed
• [Journal Article] Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment. (2009)
  • Author(s): Kim M, Ogawa M, Fujita Y, Yoshikawa Y, Nagai T, Koyama T, Nagai S, Lange A, Fassler R, Sasakawa C.
  • Journal Title: Nature. 459 Pages: 578-582
  • Peer Reviewed
• [Journal Article] Identification and characterization of a novel type III secretion system in trh-positive Vibrio parahaemolyticus strain TH3996 reveal genetic lineage and diversity of pathogenic machinery beyond the species level. (2009)
  • Author(s): Okada N, Iida T, Park K.-S, Goto N, Yasunaga T, Hiyoshi H, Matsuda S, Kodama T, Honda T.
  • Journal Title: Infect Immun. 77 Pages: 904-913
  • Peer Reviewed
• [Journal Article] Differential replication dynamics for large and small Vibrio chromosomes affect gene dosage, expression and location. (2008)
  • Author(s): Dryselius R, Izutsu K, Honda T, Iida T.
  • Journal Title: BMC Genomics 9 Pages: 559
  • Peer Reviewed
• [Journal Article] Precise region and the character of the pathogenicity island in clinical Vibrio parahaemolyticus strains. (2008)
  • Author(s): Sugiyama T, Iida T, Izutsu K, Park K.-S, Honda T.
  • Journal Title: J.Bacteriol. 190 Pages: 1835-1837
  • Peer Reviewed
• [Journal Article] A bacterial Effector Targets Mad2L2, an APC Inhibitor, to Modulate Host Cell Cycling. (2007)
  • Author(s): Iwai H, Kim M, Yoshikawa Y, Ashida H, Ogawa M, Fujita Y, Muller D, Kirikae T, Jackson P.K, Kotani S.Sasakawa C.
  • Journal Title: Cell 130 Pages: 611-623
  • Peer Reviewed
• [Journal Article] Shigella IpgB1 promotes bacterial entry through the ELMO-Dock 180 machinery. (2007)
  • Author(s): Handa Y, Suzuki M, Ohya K, Iwai H, Ishijima N, Koleske A.J, Fukui Y, Sasakawa C.
  • Journal Title: Nat Cell Biol 9 Pages: 121-128
  • Peer Reviewed
• [Journal Article] Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus Type III secretion system 2. (2007)
  • Author(s): Kodama T, Rokuda M, Park K.-S, Cantarelli V.V, Matsuda S, Iida T, Honda T.
  • Journal Title: Cell.Microbiol. 9 Pages: 2598-2609
  • Peer Reviewed
• [Journal Article] Microtubule-severing activity of Shigella is pivotal for intercellular spreading. (2006)
  • Author(s): Yoshida S, Handa Y, Suzuki T, Ogawa M, Suzuki M, Tamai A, Abe A, Katayama E, Sasakawa C.
  • Journal Title: Science 314 Pages: 985-989
  • Peer Reviewed
• [Presentation] New insights into Shigella-gut epithelium interaction. (2010)
  • Author(s): Sasakawa, C.
  • Organizer: Embo Workshop, Emerging Themes in infectionBiology.
  • Place of Presentation: Nice, France
  • Year and Date: 2010-06-02
• [Presentation] Identification and characterization of a novel type III secretion system in trh-positive Vibrio parahaemolyticus strain TH3996 : genetic lineage and diversity of pathogenic machinery beyond the species level. (2009)
  • Author(s): Iida T.
  • Organizer: VIBRIO 2009
  • Place of Presentation: Rio de Janeiro, Brazil
  • Year and Date: 20091104-20091106
• [Presentation] Autophagy Evasion by Cytosolic Bacteria. (2009)
  • Author(s): Sasakawa, C.
  • Organizer: 109^<th> General Meeting of the American Society for Microbiology
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2009-05-18
• [Presentation] Unravelling the pathogenesis of V.parahaemolyticus by means of high throughput genomics. (2008)
  • Author(s): Iida T.
  • Organizer: The 54th Brazilian Congress of Genetics, Bahia Othon Palace Hotel
  • Place of Presentation: Salvador, Brazil
  • Year and Date: 20080916-20080919
• [Presentation] Shigella infection of intestinal mucosa and host response. (2008)
  • Author(s): Sasakawa, C.
  • Organizer: The 9^<th> Korea-Japan International Symposium on Microbiology
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2008-10-17
• [Presentation] Shigella modulerar immunsvaret. (2008)
  • Author(s): Sasakawa, C.
  • Organizer: Swedish Microbiologist Meeting
  • Place of Presentation: Umea, Sweden
  • Year and Date: 2008-06-04
• [Presentation] Pathogenic mechanism and genomics of Vibrio parahaemolyticus. (2007)
  • Author(s): Iida T.
  • Organizer: Keynote lecture, VIBRIO 2007
  • Place of Presentation: Institut Pasteur, Paris
  • Year and Date: 20071128-20071201
• [Presentation] Shigella Infection Maneuver. (2007)
  • Author(s): Sasakawa, C.
  • Organizer: MBO-FEMS-LEOLOLDINA Symposium
  • Place of Presentation: Kloster Banz, Germany
  • Year and Date: 2007-10-12
• [Presentation] Shigella intracellular survival strategy. (2006)
  • Author(s): Sasakawa, C.
  • Organizer: Interface of Cell Biology and Cellular Microbiology of European Science Foundation Meeting 2006
  • Place of Presentation: San Felie de Guixols, Spain
  • Year and Date: 20060923-20060928
• [Book] Vibrio parahaemolyticus.Genomes of Food-and Water-Borne Pathogens (2010)
  • Author(s): Izutsu K, Iida T.
  • Total Pages: 77-84
  • Publisher: ASM Press
• [Book] Comparative genomics : the genome configuration and the driving forces in the evolution of vibrios.The Biology of Vibrios. (2006)
  • Author(s): Iida T, Kurokawa K.
  • Total Pages: 67-75
  • Publisher: ASM Press
• [Book] Vibrio parahaemolyticus.The Biology of Vibrios. (2006)
  • Author(s): Iida T, Park K.-S, Honda T.
  • Total Pages: 340-348
  • Publisher: ASM Press
• [Remarks] ホームページ等
  • URL: http://www.ims.u-tokyo.ac.jp/bac/hp/mainpage.html