Growth and pathogenicity of helminthes

2010 Fiscal Year Final Research Report

• Project Area: Matrix of Infection Phenomena
• Project/Area Number: 18073004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: KITA Kiyoshi The University of Tokyo, 大学院・医学系研究科, 教授 (90134444)
• Project Period (FY): 2006 – 2010
• Keywords: 感染症 / 寄生適応 / 低酸素適応 / ミトコンドリア / フマル酸呼吸 / 複合体II / 薬剤開発

Research Abstract

Parasites are good model system for the study of biological adaptation in eukaryotic organisms. Especially, to study the fundamental functions such as energy metabolisms common among the living beings and to understand a mechanism of the biological reactions, parasite is one of the best systems.
By the study using Ascaris suum which resides in the host small intestine where oxygen tension is quite low, we found that NADH-fumarate reductase system (Fumarate respiration) plays an important role in the adaptation to hypoxic environment. Further analysis revealed that fumarate respiration functions in the host human cells in hypoxic condition as well as other helminthes

Research Products

• [Journal Article] Contribution of the FAD and quinone binding sites to the production of reactive oxygen species (ROS) from Ascaris suum mitochondrial complex II. (2010)
  • Author(s): Paranagama, M.P., Sakamoto, K., Amino, H., Awano, M., Miyoshi, H., Kita, K.
  • Journal Title: Mitochondrion 10 Pages: 158-165
  • Peer Reviewed
• [Journal Article] Crystallization of mitochondrial rhodoquinol-fumarate reductase from the parasitic nematode Ascaris suum with specific inhibitor, flutolanil. (2009)
  • Author(s): Osanai, A., Harada, S., Sakamoto, K., Shimizu, H., Inaoka, D. K., Kita, K.
  • Journal Title: Acta Crystallographica F65 Pages: 941-944
  • Peer Reviewed
• [Journal Article] Regulation of succinate-ubiquinone reductase and fumarate reductase activities in human complex II by phosphorylation of its flavoprotein subunit. (2009)
  • Author(s): Tomitsuka, E., Kita, K., Esumi, H.
  • Journal Title: Proc.Jap.Acad. B85 Pages: 258-265
  • Peer Reviewed
• [Journal Article] Freezing survival of the nematode Caenorhabditis elegans in the presence of a cryoprotectant. (2009)
  • Author(s): Hayashi, M., Oguma, T., Amino, H., Kita, K., Murase, N.
  • Journal Title: Cryobiology and Cryotechnology 55 Pages: 9-22
  • Peer Reviewed
• [Journal Article] Screening of detergents for solubilization, purification and crystallization of membrane proteins : a case study on succinate : ubiquinone oxidoreductase from Escherichia coli. (2008)
  • Author(s): Shimizu, H., Nihei, C., Inaoka, D. K., Mogi, T., Kita, K., Harada, S.
  • Journal Title: Acta Crystallographica F64 Pages: 858-862
  • Peer Reviewed
• [Journal Article] Change of subunit composition of mitochondrial complex II (Succinate-ubiquinone reductase/Quinol-fumarate reductase) in Ascaris suum during the migration in the experimental host. (2008)
  • Author(s): Iwata F., Shinjyo N., Amino H., Sakamoto K., Islam M.K., Tsuji N., Kita K.
  • Journal Title: Parasitol.Int. 57 Pages: 54-61
  • Peer Reviewed
• [Journal Article] Anaerobic NADH-Fumarate Reductase System Is Predominant in the Respiratory Chain of Echinococcus multilocularis, Providing a Novel Target for the Chemotherapy of Alveolar Echinococcosis. (2008)
  • Author(s): Matsumoto J., Sakamoto K., Shinjyo N., Kido Y., Yamamoto N., Yagi K., Miyoshi H., Nonaka N., Katakura K., Kita K., Oku Y.
  • Journal Title: Antimicrob.Agents.Chemother. 52 Pages: 164-170
  • Peer Reviewed
• [Journal Article] Parasitology in Japan : Advances in drug discovery and biochemical studies. (2007)
  • Author(s): Kita, K., Shiomi K., Omura, S.
  • Journal Title: Trends in Parasitol. 23 Pages: 223-229
  • Peer Reviewed
• [Journal Article] Ukulactones A and B, new NADH-fumarate reductase inhibitors produced by Penicillium sp.FKI-3389.
  • Author(s): Mori, M., Morimoto, H., Kim, Y-P., Ui, H., Nonaka, K., Masuma, R., Sakamoto, S., Kita, K., Tomoda, H., Shiomi, K., mura, S.
  • Journal Title: Tetrahedron 掲載確定(印刷中)
  • Peer Reviewed
• [Presentation] エキノコックスミトコンドリア呼吸鎖複合体IIassembly factorのクローニング (2010)
  • Author(s): 遠海重裕、坂元君年、後藤美穂、松本淳、八木欣平、片倉賢、奥祐三郎、藤田修、野崎智義、北潔
  • Organizer: 第70回日本寄生虫学会東日本支部大会
  • Year and Date: 2010-10-02
• [Presentation] Novel enzymes of parasite mitochondria essential for their survival (2010)
  • Author(s): Kita K.
  • Organizer: The 10th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji (Hyogo)
  • Year and Date: 2010-09-08
• [Presentation] Ascaris suum quinol fumarate reductase can produce high amount of reactive oxygen species. (2008)
  • Author(s): Madhavi Paranagama, Kimitoshi Sakamoto, Kiyoshi Kita
  • Organizer: 第81回日本生化学会大会第・31回日本分子生物学会年会合同大会
  • Year and Date: 2008-12-10
• [Presentation] 寄生虫の生活環におけるダイナミックなエネルギー代謝の変動 (2008)
  • Author(s): 北潔
  • Organizer: 第81回日本生化学会大会第・31回日本分子生物学会年会合同大会
  • Year and Date: 2008-12-09
• [Book] 微生物資源国際戦略 (2009)
  • Author(s): 北潔
  • Total Pages: 448
  • Publisher: サイエンスフォーラム社
• [Remarks] ホームページ
  • URL: http://www.biomedchem.m.u-tokyo.ac.jp/
• [Patent(Industrial Property Rights)] 内部寄生虫防除剤 (2011)
  • Inventor(s): 北潔、諏訪明之
  • Industrial Property Rights Holder: 東京大学、日本農薬株式会社
  • Industrial Property Number: 特許権,特願2011-045042
  • Filing Date: 2011-03-02