Molecular basis of replication and pathgenecity of herpes simplex viruses

2008 Fiscal Year Self-evaluation Report

• Project Area: Matrix of Infection Phenomena
• Project/Area Number: 18073007
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University
• Principal Investigator: NISHIYAMA Yukihiro Nagoya University, 大学院医学系研究科, 教授 (60115615)
• Project Period (FY): 2006 – 2010
• Keywords: DNAウイルス / 単純ヘルペスウイルス / ウイルス遺伝子機能 / ウイルス病原性

Research Abstract

本研究は「ウイルス研究マトリックス」の視点から、DNAウイルスの中でもヘルペスウイルスを取り上げて研究を推進する。単純ヘルペスウイルス(HSV)はヘルペスウイルス科のプロトタイプとして最も分子的理解が進んでいるが、増殖、病原性発現機構ともに不明な点が数多く残されている。また、HSVは少なくとも74個の遺伝子を保有するが、半数以上の遺伝子機能については未だよくわかっていないのが現状である。本研究の目的は、ウイルスの増殖機構、病原性発現機構にこれらの遺伝子がどのような役割を果たしているのかを明らかにすることである。

Research Products

• [Journal Article] Microarray analysis of transcriptional responses to infection by herpes simplex virus types 1 and 2 and their US3-deficient mutants (2008)
  • Author(s): Kamakura, M., Nawa, A., Ushijima, Y., Goshima, F., Kawaguchi, Y., Kikkawa, F., Nishiyama, Y.
  • Journal Title: Microbes and Infection 10 Pages: 405-413
  • Peer Reviewed
• [Journal Article] Herpes simplex virus induces extensive modification and dynamic relocalisation of the nuclear mitotic apparatus protein (NuMA) in interphase cells (2008)
  • Author(s): Yamauchi, Y., Kiriyama, K., Kimura, H. and Nishiyama, Y.
  • Journal Title: Journal of Cell Science 121 Pages: 2087-2096
  • Peer Reviewed
• [Journal Article] Herpes simplex virus type 2 UL56 interacts with the ubiquitin ligase nedd4 and increases its ubiquitination. (2008)
  • Author(s): Ushijima Y, Koshizuka T, Goshima F, Kimura H, Nishiyama Y.
  • Journal Title: Journal of Virology 82 Pages: 5220-5233
  • Peer Reviewed
• [Journal Article] The UL14 tegument protein of herpes simplex virus type 1 (HSV-1) is required for efficient nuclear transport of the alpha transinducing factor VP16 and viral capsids. (2008)
  • Author(s): Yamauchi Y, Kiriyama K, Kubota N, Kimura H, Usukura J, Nishiyama Y.
  • Journal Title: Journal of Virology 82 Pages: 1094-1106
  • Peer Reviewed
• [Journal Article] Determination and analysis of the DNA sequence of highly attenuated herpes simplex virus type 1 mutant HF10, a potential oncolytic virus. (2007)
  • Author(s): Ushijima Y, Luo C, Goshima F, Yamauchi Y, Kimura H, Nishiyama Y.
  • Journal Title: Microbes and Infection 9(2) Pages: 142-149
  • Peer Reviewed
• [Journal Article] Oncolytic virotherapy with an HSV amplicon vector expressing granulocyte-macrophage colony-stimulating factor using the replication-competent HSV type I mutant HF10 as a helper. (2007)
  • Author(s): Kohno, S., Luo, C., Nawa, A., Fujimoto, Y., Waranabe, D., Goshima, F., Tsurumi, T. and Nishiyama, Y.
  • Journal Title: Cancer Gene Therapy 14 Pages: 918-926
  • Peer Reviewed
• [Journal Article] Suitability of a US3-inactivated HSV mutant(L1BR1) as an oncolytic-virus for pancreatic cancer therpay. (2007)
  • Author(s): Kasuya, H.,Nishiyama, Y., Nomoto, S., Goshima, F., Takeda, S., Watanabe, I., Nomura, N., Shikano, T., Fujii, T., Kanazumi, N. and Nakao, A.
  • Journal Title: Cancer Gene Therapy 14 Pages: 533-542
  • Peer Reviewed
• [Journal Article] Herpes simplex virus US3 protein kinase regulates virus-induced apoptosis in olfactory and vomeronasal chemosensory neurons in vivo. (2006)
  • Author(s): Mori, I., Goshima, F., Watanabe, D., Ito, H., Koide, N., Yoshida, T., Liu, B., Kimura, Y., Yokochi, T. and Nishiyama, Y.
  • Journal Title: Microbes and infection 8 Pages: 1806-1812
  • Peer Reviewed
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/virus