2010 Fiscal Year Final Research Report
An experimental platform for membrane protein analysis by using micro/nano machining technologies
| Project Area | Innovative nanoscience of supermolecular motor proteins working in biomembranes |
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| Project/Area Number |
18074001
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKEUCHI Shoji The University of Tokyo, 生産技術研究所, 准教授 (90343110)
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| Co-Investigator(Kenkyū-buntansha) |
MUNEYUKI Eiro 中央大学, 理工学部, 教授 (80219865)
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| Project Period (FY) |
2006 – 2010
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| Keywords | 膜タンパク質 / MEMS / マイクロ流体デバイス |
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| Research Abstract |
Membrane proteins play very important roles in cells. Here, I will propose our microfluidic technologies to form lipid bilayer membrane and vesicles. These technologies are useful for producing a membrane protein chip : an array of single-species-specific membrane proteins reconstituted into (1) planar lipid bilayers formed in microfabricated holes and channels and (2) giant vesicles (giant liposomes) Planar lipid bilayers are formed at apertures, 100 micron in diameter, by flowing lipid organic solution and buffer alternately into an integrated microfluidic channel. Using this technique, multiple lipid bilayers are formed simultaneously in a single chip, and channel currents through peptide ion channels was recorded in parallel. Regarding giant vesicles, "blowing" a planar lipid bilayer and deforming it into vesicles (like blowing soap bubbles). We have also developed a simple method to make an array of the vesicles on a microfluidic chip. We believe that these devices are useful for an efficient and rapid analysis of single-species-specific membrane proteins.
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