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2010 Fiscal Year Final Research Report

Single-molecule study on rotary catalysis of FoF1-ATP synthase

Planned Research

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Project AreaInnovative nanoscience of supermolecular motor proteins working in biomembranes
Project/Area Number 18074005
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

NOJI Hiroyuki  Osaka University, 産業科学研究所, 招へい教授 (00343111)

Co-Investigator(Kenkyū-buntansha) TABATA Kazuhito  東京大学, 工学系研究科, 助教 (50403001)
IINO Ryota  東京大学, 工学系研究科, 講師 (70403003)
Co-Investigator(Renkei-kenkyūsha) IDE Toru  大阪大学, 生命機能研究科, 准教授 (60231148)
Project Period (FY) 2006 – 2010
Keywords1分子計測 / 分子モーター / 生物物理
Research Abstract

We developed a wide variety of nanobiotechnogoly, taking the advantageous features of this interdisciplinary research project. Included are single-molecule imaging method integrated with planner bilayer system, novel microdevices for single-molecule detection, novel fluorescent probe, and novel torque-determination method based on fluctuation theorem. In addition, we revealed many fundamental insights about the mehcano-chemical coupling mechanism of F_oF_1-ATPase, such as the establishment of reaction scheme of F_1, mechanical modulation of reaction equilibrium, and structural basis of cooperativity among 3 torque-generating beta subunits.

  • Research Products

    (53 results)

All 2011 2010 2009 2008 2007 2006 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (20 results) Book (20 results) Remarks (1 results)

  • [Journal Article] Simple dark-field microscopy with nanometer spatial precision and microsecond temporal resolution.2010

    • Author(s)
      Ueno H, Nishikawa S, Iino R, Tabata KV, Sakakihara S, Yanagida T, Noji H.
    • Journal Title

      Biophys.J. 98:0

      Pages: 2014-2023

    • Peer Reviewed
  • [Journal Article] Fluctuation theorem applied to F1-ATPas e.2010

    • Author(s)
      Hayashi K, Ueno H, Iino R, Noji H.
    • Journal Title

      Phys.Rev.Lett. 104

      Pages: 218103

    • Peer Reviewed
  • [Journal Article] Phosphate-r elease in F1-ATPase catalytic cycle follows ADP release.2010

    • Author(s)
      Watanabe R, Iino R, Noji H.
    • Journal Title

      Nat.Chem.Biol. 6

      Pages: 814-820

    • Peer Reviewed
  • [Journal Article] A single-molecule enzymatic assay in a directly accessible femtoliter droplet array.2010

    • Author(s)
      Sakakihara S, Araki S, Iino R, Noji H.
    • Journal Title

      Lab Chip. 10

      Pages: 3355-3362

    • Peer Reviewed
  • [Journal Article] Simultaneous Optical and Electrical Single Channel Recordings on a PEG Glass2010

    • Author(s)
      Ide T, Takeuchi Y, Noji H Tabata KV.
    • Journal Title

      Langmuir. 26

      Pages: 8540-8543

    • Peer Reviewed
  • [Journal Article] Acceleration of the ATP-binding rate of F1-ATPase by forcible forward rotation2009

    • Author(s)
      Iko, Y., Tabata, K.V., Sakakihara, S., Nakashima, T., Noji H.
    • Journal Title

      FEBS Lett. 583

      Pages: 3187-3191

    • Peer Reviewed
  • [Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane2009

    • Author(s)
      Tabata, K.V., Sato, K., Ide, T., Nishizaka, T., Nakano, A., Noji H.
    • Journal Title

      EMBO J. 28

      Pages: 3279-3289

    • Peer Reviewed
  • [Journal Article] Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-bas ed genetically encoded indicators2009

    • Author(s)
      Imamura, H., Huynh Nhat, K.P., Togawa, H., Saito, K., Iino, R., Kato-Yamada, Y., Nagai, T., Noji H.
    • Journal Title

      Proc Natl Acad Sci USA. 106

      Pages: 15651-15656

    • Peer Reviewed
  • [Journal Article] Single-molecule study on the temperature -sensitive reaction of F1-ATPase with a hybrid F1 carrying a single β (E190D)2009

    • Author(s)
      Enoki, S., Watanabe, R., Iino, R., Noji H.
    • Journal Title

      J Biol Chem. 284

      Pages: 23169-23176

    • Peer Reviewed
  • [Journal Article] Mechanism of inhibition by C-terminal α-helices of the ε subunit of Escherichia coli FoF1-ATP synthase2009

    • Author(s)
      Iino, R., Hasegawa, R., Tabata, K.V., Noji H.
    • Journal Title

      J Biol Chem 284

      Pages: 17457-17464

    • Peer Reviewed
  • [Journal Article] Correl ation between the conformational states of F1-ATPase as determined from its crystal structure and single-molecule rotation2008

    • Author(s)
      Okuno D, Fu j isawa R , I ino R, Hirono-Hara , Imamura H, Noji H.
    • Journal Title

      Proc.Natl.Acad.Soc.U.S.A. 105

      Pages: 20722-20727

    • Peer Reviewed
  • [Journal Article] Temperature-sensitive reaction intermediate of F1-ATPase.2008

    • Author(s)
      Watanabe R, Iino R, Shimabukuro K, Yoshida M, Noji H.
    • Journal Title

      EMBO Rep. 9(1):8

      Pages: 84-90

    • Peer Reviewed
  • [Presentation] Femto-liter Reactor Array for Single-molecule Bioanalysis2011

    • Author(s)
      Hiroyuki Noji
    • Organizer
      PITTCON 2011(招待講演)
    • Place of Presentation
      Georgia World Congress Center (アメリカ・アトランタ)(口頭発表)
    • Year and Date
      2011-03-15
  • [Presentation] STRUCTURAL FLUCTUATION AND CATALYTIC FUNC TION OF F1-ATPASE.2011

    • Author(s)
      Rikiya Watanabe, Kumiko Hayashi, Hiroshi Ueno, Hiroyuki Noji
    • Organizer
      Biophysical Society 55th Annual Meeting,
    • Place of Presentation
      Baltimore Convention Center (バルチモア・アメリカ)(口頭発表)
    • Year and Date
      2011-03-07
  • [Presentation] Single-molecule studies on the fluctuation and function of a rotary motor protein ATP synthase2010

    • Author(s)
      Ryota Iino
    • Organizer
      The 4th International Symposium "Molecular Science of Fluctuations toward Biological Functions
    • Place of Presentation
      ホテルピアザびわ湖(大津)(口頭発表)
    • Year and Date
      2010-11-30
  • [Presentation] Culture, detection, and recovery of the antibiotic-tolerant persister bacteria in the directly accessible microchamber array2010

    • Author(s)
      Iino R., Hayama K., Sakakihara S., Noji H.
    • Organizer
      14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2010)
    • Place of Presentation
      University of Groningen(フローニンゲン・オランダ)(口頭発表)
    • Year and Date
      2010-10-05
  • [Presentation] Direct observation of steps in c-ring rotation of Escherichia coli FoF1-ATP synthase2010

    • Author(s)
      Ryota Iino, Khek-Chian Tham, Kazuhito V.Tabata, Hiroshi Ueno, Hiroyuki Noji
    • Organizer
      16th European Bioenergetics Conference
    • Place of Presentation
      University of Warsaw(ワルシャワ・ポーランド)(口頭発表)
    • Year and Date
      2010-07-18
  • [Presentation] Mechanochemistry of F1-ATPase Moter Protein2010

    • Author(s)
      Hiroyuki Noji
    • Organizer
      ISMSC2010
    • Place of Presentation
      奈良県新公会堂(奈良)(口頭発表)
    • Year and Date
      2010-06-09
  • [Presentation] FemtoliterChamber Array for Single Molecular Bioassay2010

    • Author(s)
      Hiroyuki Noji
    • Organizer
      ISM M2010
    • Place of Presentation
      KUST(Hong Kong)(口頭発表)
    • Year and Date
      2010-05-29
  • [Presentation] Imaging of Intracellular ATP Using FRET-Based Indicators, Intern ational Symposium of Joint Research Network on Advanced Materials and Devices2010

    • Author(s)
      Hiroyuki Noji
    • Place of Presentation
      Sapporo (Japan)(口頭発表)
    • Year and Date
      2010-03-26
  • [Presentation] Single-Molecule Imaging of Ion-Transporting Rotary Motor Protein2010

    • Author(s)
      Ryota Iino, Khek-Chian Tham, Kazuhito V.Tabata, Hiroshi Ueno, Hiroyuki Noji
    • Organizer
      International Symposium of Joint Research Network on Advanced Materials and Devices
    • Place of Presentation
      Sapporo (Japan)(口頭発表)
    • Year and Date
      2010-03-25
  • [Presentation] Completion of the chemomechanical coupling scheme of F1-ATPase2009

    • Author(s)
      Hiroyuki Noji
    • Organizer
      Single Molecule Biology Symposium (2nd Kanazawa Bio-AFM workshop)
    • Place of Presentation
      Osaka (Japan)(口頭発表)
    • Year and Date
      2009-12-15
  • [Presentation] Visualization of COPII vesicle formation process reconstituted in the artifical lipid bilayer.2009

    • Author(s)
      Kazuhito Tabata, Ken Sato, Toru Ide, Takayuki Nshizaka, Akihiko Nakano, Hiroyuki Noji
    • Organizer
      The 47th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Tokushima (Japan)(口頭発表)
    • Year and Date
      2009-10-30
  • [Presentation] 36-degree stepping rotation of FoF1-ATP synthase2009

    • Author(s)
      Hiroyuki Noji, Ryota Iino
    • Organizer
      International Symposium "Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes"
    • Place of Presentation
      Kyoto (Japan)(口頭発表)
    • Year and Date
      2009-09-09
  • [Presentation] Single molecule biophsicis of F1-ATPase : Mechanical mondulation odreaction rate constants and equilibrium constants by F1-ATPase2009

    • Author(s)
      野地博行
    • Organizer
      第6回Asian biophysics Association Symposium
    • Place of Presentation
      HKUST(香港)(口頭発表)
    • Year and Date
      2009-01-11
  • [Presentation] Femtoliter chamber for Single-molecule and single-cell analysis2009

    • Author(s)
      野地博行
    • Organizer
      第4回IEEE-NEMS09
    • Place of Presentation
      中国(シンセン)(口頭発表)
    • Year and Date
      2009-01-06
  • [Presentation] Single molecule studies on F1-ATPase molecular motor2008

    • Author(s)
      野地博行
    • Organizer
      第16回International Colloquium on Scanning Probe Microscopy
    • Place of Presentation
      伊豆・熱川(口頭発表)
    • Year and Date
      2008-12-13
  • [Presentation] Single-molcule Imaging of the transport-vesicle formation mediated by COPII system reconstituted on a planner bilayer system2008

    • Author(s)
      野地博行
    • Organizer
      第17回CDB会議(野地オーガナイザー)
    • Place of Presentation
      理科学研究所(神戸)(口頭発表)
    • Year and Date
      2008-10-14
  • [Presentation] Mechanical modulation of ATP-binding and hydrolysis by single F1-ATPase molecule2008

    • Author(s)
      野地博行
    • Organizer
      MPSA2008
    • Place of Presentation
      札幌(口頭発表)
    • Year and Date
      2008-08-29
  • [Presentation] Single Molecule Studies on F1-ATPase, The 6th JSPS Forum in France2007

    • Author(s)
      野地博行
    • Organizer
      Chemical and physical nanobiology for me dicine
    • Place of Presentation
      Strasbourg(France)(口頭発表)
    • Year and Date
      2007-11-23
  • [Presentation] Single Molecule Studies on F1-ATPase2007

    • Author(s)
      野地博行
    • Organizer
      Autumn School for PhD students and young researcher (NAMIS) 2007
    • Place of Presentation
      Tokyo(Japan)(口頭発表)
    • Year and Date
      2007-11-05
  • [Presentation] Single Molecule Studies on F1-ATPase2007

    • Author(s)
      野地博行
    • Organizer
      Asian and Pacific Biomechanics (AP Biomech) 2007
    • Place of Presentation
      Tokyo(Japan)(口頭発表)
    • Year and Date
      2007-11-05
  • [Book] In "Encyclopedia of Biophysics" edited by Roberts G.C.(Springer (India))2011

    • Author(s)
      Okuno D, Iino R, Noji H.
    • Publisher
      Fundamental Properties and Structure of F1-ATPase.(in press)
  • [Book] In "Molecular Biology of Assemblies and Machines" edited by Baumeister W, Johnson L, Perham R, and Steven A.(Garland (England)2011

    • Author(s)
      Noji H, Iino R.
    • Publisher
      ATP synthase-Structure and dynamics of the smallest rotary motor proteins.(in press)
  • [Book] 「第4章第1節第1項生体膜超分子モーター-細胞の回転エネルギー変換装置:ATP合成酵素」超分子サイエンス&テクノロジー2011

    • Author(s)
      飯野亮太、田端和仁、野地博行
    • Total Pages
      851-859
    • Publisher
      NTS
  • [Book] 細胞の回転エネルギー変換装置.「超分子サイエンス」2009

    • Author(s)
      野地博行, 田端和仁, 飯野亮太
    • Publisher
      NTS、編集中(in press)
  • [Book] 分子機械.「基礎ナノバイオテクノロジー」,第4章第6節(編集中)2009

    • Author(s)
      飯野亮太 野地博行
    • Publisher
      培風館(in press)
  • [Book] 超微笑溶液チャンバーを利用した1分子バイオアッセイ2008

    • Author(s)
      野地博行
    • Publisher
      丸善
  • [Book] 分子マシンに新たな扉が開かれた2008

    • Author(s)
      金原数、竹内昌治、竹内正之、野地博行
    • Publisher
      化学同人
  • [Book] F1-ATPaseに見る回転分子モーターのナノバイオロジー2008

    • Author(s)
      野地博行、田端和仁
    • Publisher
      化学同人
  • [Book] 最新分子マシンナノで働く"高度な機械"を目指して2008

    • Author(s)
      飯野亮太
    • Publisher
      化学同人
  • [Book] 未来材料「超微小反応チャンバーを用いた高感度バイオアッセイ」2007

    • Author(s)
      飯野亮太, Liza Lam, 野地博行
    • Total Pages
      68
    • Publisher
      未来材料
  • [Book] 生物物理学ハンドブック2007

    • Author(s)
      野地博行
    • Total Pages
      680
    • Publisher
      朝倉書店
  • [Book] ナノバイオ計測の実際2007

    • Author(s)
      野地博行
    • Total Pages
      206
    • Publisher
      講談社
  • [Book] ナノバイオ大辞典2007

    • Author(s)
      野地博行
    • Total Pages
      636
    • Publisher
      テクノシステム
  • [Book] 蛋白質核酸酵素,Vol.52 No.42007

    • Author(s)
      野地博行
    • Total Pages
      396
    • Publisher
      共立出版
  • [Book] ナノテクのためのバイオ入門2007

    • Author(s)
      野地博行
    • Total Pages
      224
    • Publisher
      共立出版
  • [Book] 「第3章磁性ビーズを用いた回転分子モーターの研究」,磁性ビーズのバイオ・環境技術への応用展開2006

    • Author(s)
      野地博行
    • Total Pages
      310
    • Publisher
      シーエムシー出版
  • [Book] 蛋白質核酸酵素(「1分子からシステム再構成へ生化学的再構成と1分子生物物理の融合をめざして」,Vol.51 No.12)2006

    • Author(s)
      野地博行
    • Total Pages
      100
    • Publisher
      共立出版
  • [Book] 超微小反応チャンバーで生体回転分子モーターの作動機構を探る2006

    • Author(s)
      飯野亮太, 野地博行
    • Total Pages
      80
    • Publisher
      (株)エヌ・ティー・エス,未来材料6
  • [Book] 学術月報(「Let's enjoy Science!」,Vol.59 No.5)2006

    • Author(s)
      野地博行
    • Total Pages
      93
    • Publisher
      日本学術振興会
  • [Book] Proceedings of 10th International Conference on Miniturized Systems for Chemistry and Life Sciences2006

    • Author(s)
      Iino, R., Rondelez, Y., Yoshida, M. Noji H.
    • Total Pages
      1591
    • Publisher
      Micro-Total Analysis Systems
  • [Remarks] ホームページ等

    • URL

      http://www.nojilab.t.u-tokyo.ac.jp/index.html

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Published: 2012-02-13   Modified: 2016-04-21  

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