2010 Fiscal Year Final Research Report
Single-molecule study on rotary catalysis of FoF1-ATP synthase
| Project Area | Innovative nanoscience of supermolecular motor proteins working in biomembranes |
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| Project/Area Number |
18074005
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Osaka University |
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Principal Investigator |
NOJI Hiroyuki Osaka University, 産業科学研究所, 招へい教授 (00343111)
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| Co-Investigator(Kenkyū-buntansha) |
TABATA Kazuhito 東京大学, 工学系研究科, 助教 (50403001)
IINO Ryota 東京大学, 工学系研究科, 講師 (70403003)
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| Co-Investigator(Renkei-kenkyūsha) |
IDE Toru 大阪大学, 生命機能研究科, 准教授 (60231148)
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| Project Period (FY) |
2006 – 2010
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| Keywords | 1分子計測 / 分子モーター / 生物物理 |
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| Research Abstract |
We developed a wide variety of nanobiotechnogoly, taking the advantageous features of this interdisciplinary research project. Included are single-molecule imaging method integrated with planner bilayer system, novel microdevices for single-molecule detection, novel fluorescent probe, and novel torque-determination method based on fluctuation theorem. In addition, we revealed many fundamental insights about the mehcano-chemical coupling mechanism of F_oF_1-ATPase, such as the establishment of reaction scheme of F_1, mechanical modulation of reaction equilibrium, and structural basis of cooperativity among 3 torque-generating beta subunits.
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Research Products
(53 results)
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[Journal Article] Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane2009
Author(s)
Tabata, K.V., Sato, K., Ide, T., Nishizaka, T., Nakano, A., Noji H.
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Journal Title
EMBO J. 28
Pages: 3279-3289
Peer Reviewed
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[Journal Article] Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-bas ed genetically encoded indicators2009
Author(s)
Imamura, H., Huynh Nhat, K.P., Togawa, H., Saito, K., Iino, R., Kato-Yamada, Y., Nagai, T., Noji H.
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Journal Title
Proc Natl Acad Sci USA. 106
Pages: 15651-15656
Peer Reviewed
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