2010 Fiscal Year Final Research Report
Molecular analysis of interaction between male-sterility inducing gene and fertility restorer in sugar beet
| Project Area | Genome Barriers in Plant Reproduction |
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| Project/Area Number |
18075001
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hokkaido University |
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Principal Investigator |
KUBO Tomohiko 北海道大学, 大学院・農学研究院, 准教授 (40261333)
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| Co-Investigator(Kenkyū-buntansha) |
MIKAMI Tetsuo 北海道大学, 大学院・農学研究院, 教授 (50133715)
ONODERA Yasuyuki 北海道大学, 大学院・農学研究院, 講師 (80374619)
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| Project Period (FY) |
2006 – 2010
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| Keywords | 生殖 / 雄性不稔性 / 核・細胞質相互作用 / ミトコンドリア / 性染色体 |
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| Research Abstract |
Cytoplasmic male sterility (CMS) has been reported in more than 140 angiosperm species. Genes responsible for CMS (S gene) are encoded in mitochondrial genomes, while plants with S gene are male fertile when the plants have nuclear genes termed restorer of fertility (Rf). Molecular mechanism of the interaction between S and Rf genes was investigated in this study using sugar beet. Protein-protein interaction was detected between preSatp6, an S gene, and Rf1. This interaction was observed only in anthers in which Rf1 was expressed. This study identified nuclear genes that were suppressed in CMS anthers. It was inferred that deleterious mutation could be inherited through pollen. This possibility was investigated using Arabidopsis mutant with defect in the genes encoding RNA polymerase. This mutation was confirmed to be transmitted through pollen. Spinach is a monoecious species with male heterogamety system. However, dioecious or hermaphroditic spinach emerges when an M gene exists. This study revealed that male determining gene and the M gene located on the same linkage group but resided in different genetic loci.
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