Key Factors of Reproductive Barriers in Pollen Tube Guidance and Double Fertilization

2008 Fiscal Year Self-evaluation Report

• Project Area: Genome Barriers in Plant Reproduction
• Project/Area Number: 18075004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nagoya University (2007-2010); The University of Tokyo (2006)
• Principal Investigator: HIGASHIYAMA Tetsuya Nagoya University, 大学院理学研究科, 教授 (00313205)
• Project Period (FY): 2006 – 2010
• Keywords: 植物 / 花粉管ガイダンス / 重複受精 / 細胞間シグナリング / 顕微細胞操作

Research Abstract

研究代表者は、胚嚢が裸出するトレニアのin vitro重複受精系やレーザーによる顕微細胞操作技術を、世界に先駆けて開発してきた。本研究の目的はこれらを駆使して、花粉管ガイダンス及び重複受精過程においてゲノム障壁の鍵因子となる、細胞間シグナリング分子を同定することである。具体的にはI)助細胞が分泌する花粉管ガイダンス分子の同定、II)花粉管の受精能獲得を制御する母体因子の同定、III)ライブイメージング解析による細胞間シグナリング分子の探索を行う。

Research Products

• [Journal Article] Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells. (2009)
  • Author(s): Okuda, S., Tsutsui, H., Sasaki, N., Higashiyama, T., (23人中23番目)
  • Journal Title: Nature 458 Pages: 357-361
  • Peer Reviewed
• [Journal Article] Distinct dynamics of HISTONE3 variants between the two fertilization products in plants. (2007)
  • Author(s): Ingouff, M., Hamamura, Y., Gourgues, M., Higashiyama, T and Berger, F.
  • Journal Title: Curr Biol. 17 Pages: 1032-1037
  • Peer Reviewed
• [Journal Article] GENERATIVECELL SPECIFIC 1 is essential for angiosperm fertilization. (2006)
  • Author(s): Mori, T., Kuroiwa, H., Higashiyama T and Kuroiwa, T.
  • Journal Title: Nature Cell Biol.. 8 Pages: 64-71
  • Peer Reviewed
• [Journal Article] Active digestion of sperm mitochondrial DNA in single living sperm revealed by optical tweezers. (2006)
  • Author(s): Nishimura, Y., higashiyama T., (8人中7番目)
  • Journal Title: Proc.Natl.Acad.Sci. USA103 Pages: 1382-1387
  • Peer Reviewed
• [Journal Article] A Species preferentiality of the pollen tube attractant derived from the synergid cell of Torenia fournieri. (2006)
  • Author(s): Higashiyama, T., (9人中1番目).
  • Journal Title: Plant Physiol. 142 Pages: 481-491
  • Peer Reviewed
• [Presentation] Pollen tube attractants derived from the synergid cell. (2008)
  • Author(s): Higashiyama, T.
  • Organizer: Frontiers of Sexual Plant Reproduction III.
  • Place of Presentation: 米国、ツーソン
  • Year and Date: 2008-10-18
• [Presentation] Behavior and Signaling in Gametophytic Interactions. (2008)
  • Author(s): Higashiyama, T.
  • Organizer: FASEB Summer Research Conference.
  • Place of Presentation: 米国、サクストンズリバー
  • Year and Date: 2008-08-12
• [Presentation] Behavior and Signaling in Gametophytic Interactions. (2008)
  • Author(s): Higashiyama, T.
  • Organizer: International Congress on Sexual Plant Reproduction.
  • Place of Presentation: ブラジル、ブラジリア
  • Year and Date: 2008-08-05
• [Presentation] Cell-cell Communication in Pollen Tube Guidance. (2006)
  • Author(s): Higashiyama, T.
  • Organizer: FASEB Summer Research Conference.
  • Place of Presentation: 米国、サクストンズリバー
  • Year and Date: 2006-08-05
• [Presentation] Intercellular Signaling in Pollen Tube Guidance. (2006)
  • Author(s): Higashiyama, T.
  • Organizer: JPSP-UPSC colloquium on Plant Development
  • Place of Presentation: スエーデン、ウメオ
  • Year and Date: 2006-06-02
• [Book] 植物の発生と形態形成. バイオサイエンス (2007)
  • Author(s): 東山哲也, 澤進一郎.
  • Total Pages: 115-121
  • Publisher: オーム社
• [Book] 受精のメカニズムをとらえた!. 植物の生存戦略 (2007)
  • Author(s): 東山哲也
  • Total Pages: 99-121
  • Publisher: 「植物の軸と情報」特定領域研究班編・朝日新聞社