Molecular analysis of the genome barriers in the early reproductive process

2010 Fiscal Year Final Research Report

• Project Area: Genome Barriers in Plant Reproduction
• Project/Area Number: 18075008
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TAKAYAMA Seiji Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (70273836)
• Project Period (FY): 2006 – 2010
• Keywords: 生殖 / シグナル伝達 / 植物 / アブラナ科 / 受粉 / ゲノム障壁

Research Abstract

We have analyzed the molecular basis for the pollen-papilla cell interactions which were thought to function as the first genome-barrier during the reproductive process in the Brassicaceae. First we found that the pollen coat contains some factor(s), which induces the rearrangement of actin filaments in the papilla cell of the same species and the secretion of Ca^<2+>-containing water to the pollen. These compatible reactions were found to be inhibited by the self-incompatibility signaling pathway. We also identified the candidate Ca^<2+>-transporter which were expected to function in this compatible pollination process.

Research Products

• [Journal Article] Trans-acting small RNA determines dominance relationships in Brassica self-incompatibility. (2010)
  • Author(s): Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Suzuki, G., Watanabe, M., Isogai, A., Takayama, S.
  • Journal Title: Nature 466 Pages: 983-986
  • Peer Reviewed
• [Journal Article] Novel self-compatible lines of Brassica rapa L. isolated from the Japanese bulk-populations. (2010)
  • Author(s): Isokawa, S., Osaka, M., Shirasawa, A., Kikuta, R., Komatsu, S., Horisaki, A., Niikura, S., Takada, Y., Shiba, H., Isogai, A., Takayama, S., Suzuki, G., Suwabe, K., Watanabe, M.
  • Journal Title: Genes Genetic Systems 85 Pages: 87-96
  • Peer Reviewed
• [Journal Article] Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene. (2010)
  • Author(s): Tsuchimatsu,T., Suwabe, K., Shimizu-Inatsugi, R., Isokawa, S., Pavlidis, P., Staedler, T., Suzuki, G., Takayama, S., Watanabe, M., Shimizu, K.K.
  • Journal Title: Nature 464 Pages: 1342-1346
  • Peer Reviewed
• [Journal Article] Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth. (2009)
  • Author(s): Iwano, M., Entani, T., Shiba, H., Kakita, M., Nagai, T., Miyawaki, A., Shoji, T., Kubo, K., Isogai, A., Takayama, S.
  • Journal Title: Plant Physiology 150 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination. (2007)
  • Author(s): Iwano, M., Shiba, H., Matoba, K., Miwa, T., Funato, M., Entani, T., Nakayama, P., Shimosato, H., Takaoka, A., Isogai, A., Takayama, S.
  • Journal Title: Plant Physiology 144 Pages: 72-81
  • Peer Reviewed
• [Journal Article] Two distinct forms of M-locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce self-incompatibility signaling in Brassica rapa. (2007)
  • Author(s): Kakita, M., Murase, K., Iwano, M., Matsumoto, T., Watanabe, M., Shiba, H., Isogai, A., Takayama, S.
  • Journal Title: Plant Cell 19 Pages: 3961-3973
  • Peer Reviewed
• [Journal Article] Characterization of the SP11/SCR high-affinity binding site involved in self-nonself recognition in Brassica self-incompatibility. (2007)
  • Author(s): Shimosato, H., Yokota, N., Shiba, H., Iwano, M., Entani, T., Che, F.-S., Watanabe, M., Isogai, A., Takayama, S.
  • Journal Title: Plant Cell 19 Pages: 107-117
  • Peer Reviewed
• [Presentation] Molecular mechanisms of self-incompatibility in the Brassicaceae. (2010)
  • Author(s): Takayama, S
  • Organizer: 21st ICSPR
  • Place of Presentation: Bristol, UK.
  • Year and Date: 2010-08-05
• [Presentation] Self-incompatibility signaling in Brassicaceae. (2010)
  • Author(s): Takayama, S
  • Organizer: International Symposium of Cell-Cell Communication in Plant Reproduction.
  • Place of Presentation: Nara, Japan
  • Year and Date: 2010-05-11
• [Presentation] Self-incompatibility signaling in Brassica. (2009)
  • Author(s): Takayama, S
  • Organizer: Symposium in 9th IPMB
  • Place of Presentation: St.Louis, USA.
  • Year and Date: 2009-10-28
• [Presentation] Fine tuning of the cytoplasmic calcium concentration is essential for pollen tube growth. (2009)
  • Author(s): Iwano, M., Isogai, A., Takayama, S.
  • Organizer: 9th IPMB
  • Place of Presentation: St.Louis, USA.
  • Year and Date: 2009-10-28
• [Presentation] Self-incompatibility system in Brassica and its application for F1-hybrid breeding. (2009)
  • Author(s): Takayama, S
  • Organizer: KRIBB-NAIST Joint Symposium
  • Place of Presentation: Jeongeup, Korea.
  • Year and Date: 2009-02-26
• [Presentation] Self-incompatibility signaling in Brassica. NAIST GCOE Int. (2008)
  • Author(s): Takayama, S
  • Organizer: Symposium
  • Place of Presentation: Nara, Japan.
  • Year and Date: 2008-11-14
• [Presentation] Actin dynamics in papilla cells of Brassica rapa during self- and cross-pollination. (2008)
  • Author(s): Iwano, M., Shiba, H., Matoba, K., Takaoka, A., Isogai, A., Takayama, S.
  • Organizer: 9th APMC
  • Place of Presentation: Jeju, Korea.
  • Year and Date: 2008-11-04
• [Book] Self-incompatibility systems in flowering plants. In "Plant Developmental Biology.Biotechnological Perspectives" (Vol. 1)(Edited by Pua, E.C., and Davey, M.R.) (2010)
  • Author(s): Kaothein-Nakayama, P., Isogai, A., Takayama, S.
  • Total Pages: 459-485
  • Publisher: Springer, Heidelberg
• [Book] Milestones identifying self-incompatibility genes in Brassica species. From old stories to new findings. In “Self-Incompatibility in Flowering Plants : Evolution, Diversity, and Mechanisms"(Edited by Franklin-Tong, V.E.) (2008)
  • Author(s): Watanabe, M., Suzuki, G., Takayama, S.
  • Total Pages: 151-172
  • Publisher: Springer, Heidelberg
• [Remarks] ホームページアドレス
  • URL: http://bsw3.naist.jp/takayama/index.html