2010 Fiscal Year Final Research Report
Analysis of diversity of ubiquitin ligase
| Project Area | Proteolysis in the Regulation of Biological Processes |
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| Project/Area Number |
18076001
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya University |
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Principal Investigator |
TAKUMI Kamura Nagoya University, 理学研究科, 教授 (40333455)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGETSUGU Hatakeyama 北海道大学, 大学院・医学研究科, 教授 (70294973)
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| Project Period (FY) |
2006 – 2010
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| Keywords | タンパク質分解 |
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| Research Abstract |
Ubiquitylation and subsequent proteasomal degradation of regulatory proteins control a variety of cellular processes. The ubiquitin conjugation to target proteins is processed by three enzymes, E1, E2, and E3. The E3s are responsible for recognizing and recruiting target proteins for polyubiquitylation. Cullin-based E3 complexes and TRIM E3 families are thought to regulate many cellular events. In this research, we try to clarify the function of Cullin-based E3 complexes and TRIM E3 families. Now we show that Cullin-based E3 complexes are responsible for regulating the function of Mrc1, Ctf4, Elongin A, p53, and Ypt53. We also identify that Lag2 negatively modulate the activity of SCF complex. Finally, we demonstrate that TRIM E3 families control the function of estrogen receptor, androgen receptor, Abi2, p53, PIAS3 and Ikkγ. These observations thus idicate that Cullin-based E3 complexes and TRIM E3 families plays an important role in a variety of cellular processes by regulating the function of many target proteins.
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[Journal Article] Non-SCF type F-box protein Roy1/Ymr258c interacts with a Ra b5-like G TPase Yp t52 a nd inhibits Ypt52 function.2011
Author(s)
Liu, Y., Nakatsukasa, K., Kotera, M., Kanada, A., Nishimura, T., Kishi, T., Mimura, S., Kamura, T.
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Journal Title
Mol.Biol.Cell 22
Pages: 1575-1584
Peer Reviewed
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[Journal Article] Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing.2010
Author(s)
Mimura, S., Yamaguchi, T., Ishii, S., Noro, E ., Katsura, T., Obuse, C ., Kamura, T.
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Journal Title
J.Biol.Chem. 285
Pages: 9858-9867
Peer Reviewed
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[Journal Article] Degradation of phosphorylated p53 by viral protein-ECSE3 ligase complex.2009
Author(s)
Sato, Y., Kamura, T., Shirata, N., Murata, T., Kudoh, A., Iwahori, S., Nakayama, S., Isomura, H., Nishiyama, Y., Tsurumi T.
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Journal Title
PLoS Pathog. 5(7)
Pages: e1000530
Peer Reviewed
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[Journal Article] Mammalian Elongin A complex mediates DNA-damage-inducedu biquitylation and degradation of Rpb1.2008
Author(s)
Yasukawa, T., Kamura, T., Kitajima, S., Conaway, RC., Conaway, JW., Aso, T.
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Journal Title
EMBO J. 27
Pages: 3256-3266
Peer Reviewed
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[Journal Article] TRIM68 regulates ligand-dependent transcription of androgen receptor in prostate cancer cells2008
Author(s)
Miyajima, N., Maruyama, S., Bohgaki, M., Kano, S., Shigemura, M., Shinohara, N., Nonomura, K., Hata keyama, S.
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Journal Title
Cancer Res. 68
Pages: 3486-3494
Peer Reviewed
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