Mechanism under lying selective substrate recognition by the ubiquitin system

2008 Fiscal Year Self-evaluation Report

• Project Area: Proteolysis in the Regulation of Biological Processes
• Project/Area Number: 18076004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University (2008-2010); Osaka City University (2006-2007)
• Principal Investigator: IWAI Kazuhiro Osaka University, 大学院生命機能研究科, 教授 (60252459)
• Project Period (FY): 2006 – 2010
• Keywords: ユビキチン / タンパク質分解

Research Abstract

ユビキチンープロテアソーム系は、時空間的な特異性を持って基質タンパク質を識別して次々ユビキチンを付加してポリユビキチン鎖を形成し、形成されたポリユビキチン鎖がプロテアソームの識別シグナルとなって、基質タンパク質を分解に至らしめる。本研究では基質ユビキチン化からプロテアソームによる分解へと至る経路の解析を進めている。

Research Products

• [Journal Article] Involvement of linear polyubiquitination of NEMO in NF-KB activation (2009)
  • Author(s): Tokunaga, F., Sakata, S.-I., Saeki, Y.,Satomi, Y., Kirisako, T., Kamei, K., Nakagawa, T., Kato, M., Murata, S., Yamaoka, S., Yamamoto, M., Akira, S.,Takao, T., Tanaka, K. and lwai K.
  • Journal Title: Nature Cell Biology 11 Pages: 123-132
• [Journal Article] Structural basis for specific cleavage of Lys63-linked polyubiquitin chains (2008)
  • Author(s): Sato, Y., Yoshikawa, A., Yamagata, A., Mimura, H., Yamashita, M., Ookata, K., Nureki, O., Iwai, K., Komada, M., and Fukai, S.
  • Journal Title: Nature 455 Pages: 358-362
• [Journal Article] The COP9/signalosome increases the efficiency of pVHL ubiquitin ligase-mediated hypoxia inducible factor-a ubiquitination (2008)
  • Author(s): Miyauchi, Y., Kato, M., Tokunaga, F., Iwai, K.
  • Journal Title: J. Biol. Chem. 283 Pages: 16622-16631
• [Journal Article] Ubiquitin is degraded by the ubiquitin system as a monomer and as part of its conjugated target (2007)
  • Author(s): Shabek, N., lwai, K., Ciechanover, A.
  • Journal Title: Biochem Biophys Res Commun 363 Pages: 425-431
• [Journal Article] Herne induces ubiquitination and degradation of the transcription factor Bachl (2007)
  • Author(s): Zenke-Kawasaki, Y., Dohi, Y., Katoh, Y., Ikura, T., lkura, M., Asahara, T., Tokunaga, F., Iwai, Igarashi, K.
  • Journal Title: Mol. Cell Biol. 27 Pages: 6962-6971
• [Journal Article] Scythe regulates apoptosis through modulating ubiquitin-mediated proteolysis of XEF1A0 (2007)
  • Author(s): Minami, R., Shimada, M., Yokosawa, H., Kawahara, H.
  • Journal Title: Biochem. J. (London) 405 Pages: 495-501
• [Journal Article] Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development (2007)
  • Author(s): Hamazaki, J., Sasaki, K., Kawahara, H., Hisanaga, S., Tanaka, K., Murata, S.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 6629-6638
• [Journal Article] A neural-specific F-box protein Fbsl functions as a chaperone suppressing glycoprotein aggregation (2007)
  • Author(s): Yoshida, Y., Murakami, A., Iwai, K., Tanaka, K.
  • Journal Title: J. Biol. Chem. 282 Pages: 7137-7144
• [Journal Article] Direct interactions between Nedd8 and ubiquitin E2 conjugating enzymes contribute to up-regulation of cullin-based E3 ligase activity (2007)
  • Author(s): Sakata, E., Yamaguchi, Y., Miyauchi, Y., Iwai, K. Chiba, T., Saeki, Y., Matsuda, N., Tanaka, K., Kato, K.
  • Journal Title: Nature Struct. Mol. Biol. 14 Pages: 167-168
• [Journal Article] Mutual regulation of conventional protein kinase C and a ubiquitin ligase complex (LUBAC) (2006)
  • Author(s): Nakamura, M., Tokunaga, F., Sakata , S.-I., Iwai, K.
  • Journal Title: Biochem. Biophys. Res. Commun. 351 Pages: 340-347
• [Journal Article] A ubiquitin ligase complex assembles linear polyubiquitin chains (2006)
  • Author(s): Kirisako, T., Kamei, K., Murata, S., Kato, M., Fukumoto, H., Kanie, K., Sano, S. Tokunaga, F., Tanaka, K., lwai, K.
  • Journal Title: EMBO J. 25 Pages: 4877-4887
• [Journal Article] Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans (2006)
  • Author(s): Shimada, M., Kanematsu, K., Tanaka, K., Yokosawa, H., Kawahara, H.
  • Journal Title: Mol. Biol. Cell 17 Pages: 5356-5371
• [Presentation] Physiological function of linear polyubiquitin chain (2009)
  • Author(s): Iwai K.
  • Organizer: Keystone Symposium "The Many Faces of Ubiquitin"
  • Place of Presentation: Copper Mountain Resort, CO, LISA
  • Year and Date: 20090111-16
• [Presentation] Prevention of TNF-a induced apoptosis by linear polyubiquitination (2008)
  • Author(s): lwai, K.
  • Organizer: The 15^<th> Takeda Science Foundation Symposium on Bioscience "Cell Death: What have we learned and what will we learn?"
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20081202-03
• [Presentation] The COP9/Signalosorne increases the efficiency of pVHL-containing ubiquitin ligase-mediated H1F-a ubiquitination (2008)
  • Author(s): Miyauchi, Y., Kato, M., Tokunaga, F., lwai K.
  • Organizer: ZOMESV
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20081111-14
• [Presentation] Linear ubiquitination of NEMO triggers NF-KB activation (2007)
  • Author(s): Tokunaga, F., Sakata, S.-I., Kirisako, T., Nakagawa, T., Murata, S. Tanaka, K. and Iwai, K.
  • Organizer: The EMBO Conference "Ubiquitin and Ubiquitin-like Modifiers in Cellular Regulation"
  • Place of Presentation: Riva Del Garda Italy
  • Year and Date: 20070922-26
• [Book] A.M. Studies of the ubiquitin proteasome system. Units 15.9 Sections 1-12. Current Protocols in Cell Biology Bonifacino J, Dasso M, Lippincott-Schwartz J, Harford J, Yamada K, Bonafacino J eds. (2006)
  • Author(s): Lorick, K., Yang, Y., Jensen, J., lwai K. and Weissman
  • Publisher: John Wiley and Sons
• [Remarks] ホームページ
  • URL: http://www.cellhio.med.osaka-u.ac.jp/