Functional interactions between hormone-sensor and osmo-sensor in the central regulation of body fluid osmolality

2010 Fiscal Year Final Research Report

• Project Area: Molecular interaction and modal shift of cellular sensors
• Project/Area Number: 18077006
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: UETA Yoichi University of Occupational and Environmental Health, Japan, 医学部, 教授 (10232745)
• Co-Investigator(Kenkyū-buntansha): NAGATOMO Toshihisa 産業医科大学, 大学病院, 准教授 (50258604) ; FUJIHARA Hiroaki 徳島大学, 大学院・ヘルスバイオサイエンス部, 助教 (10369051) ; YOKOYAMA Toru 自治医科大学, 医学部, 助教 (80425321)
• Project Period (FY): 2006 – 2010
• Keywords: セルセンサー / 浸透圧 / バゾプレッシン

Research Abstract

The purpose of the study was to examine the relationship between sensing the hormones (hormone-sensor) secreted by changes of osmolality and sensing the osmolality (osmo-sensor). In particular, we examined arginine vasopressin (AVP) system and drinking behavior. We obtained significant results as follow. (1) In in vitro brain slice preparation including the supraoptic nucleus (SON) that contain AVP neurons whole-cell patch-clamp recordings revealed effects of physiologically active substances on the excitatory and inhibitory synaptic currents in the SON. (2) Whole-cell patch-clamp recordings from an isolated single AVP-eGFP neuron revealed the electrophysiological properties. The diurnal changes of the expression of the several genes and electrophysiological properties were determined in the AVP-eGFP neurons in the suprachiasmatic nucleus that is known to be a circadian center. (3) Central effects of angiotensin II etc on drinking behavior were examined in rats.

Research Products

• [Journal Article] Possible contribution of pannexin channel to ATP-induced currents in vitro in vasopressin neurons isolated from the rat supraoptic nucleus. (2011)
  • Author(s): Ohbuchi, T., Yokoyama, T., Saito, T., Ohkubo, J., Suzuki, H., Ishikura, T., Katoh, A., Fujihara, H., Hashimoto, H., Suzuki, H., Ueta, Y.
  • Journal Title: Brain Research in press
  • Peer Reviewed
• [Journal Article] V_2 receptor-mediated autocrine role of somatodendritic release of AVP in rat vasopressin neurons under hypo-osmotic conditions. (2011)
  • Author(s): Sato, K., Numata, T., Saito, T., Ueta, Y., Okada, Y.
  • Journal Title: Science Signaling 4(157)(Cover article of the issue) Pages: ra5
  • Peer Reviewed
• [Journal Article] Allylisothiocyanates and cinnamaldehyde potentiate miniature excitatory postsynaptic inputs in the sipraoptic nucleus in rats. (2011)
  • Author(s): Yokoyama, T., Ohbuchi, T., Saito, T., Fujihara, H., Minami, K., Nagatomo, T., Uezono, Y., Ueta, Y.
  • Journal Title: European Journal of Pharmacology 655(1-3) Pages: 31-37
  • Peer Reviewed
• [Journal Article] Hypothalamic vasopressin response to stress and various physiological stimuli : Visualization in transgenic animal models. (2011)
  • Author(s): Ueta, Y., Dayanithi, G., Fujihara, H.
  • Journal Title: Hormones and Behavior 59(2) Pages: 221-226
  • Peer Reviewed
• [Journal Article] Diurnal changes of arginine vasopressin-enhanced green fluorescent protein fusion transgene expression in the rat suprachiasmatic nucleus. (2010)
  • Author(s): Maruyama, T., Ohbuchi, T., Fujihara, H., Shibata, M., Mori, K., Murphy, D., Dayanithi, G., Ueta, Y.
  • Journal Title: Peptides 31(11) Pages: 2089-2093
  • Peer Reviewed
• [Journal Article] Modulators of BK and SK channels alter electrical activity in vitro in single vasopressin neurons isolated from the rat supraoptic nucleus. (2010)
  • Author(s): Ohbuchi, T., Yokoyama, T., Saito, T., Suzuki, H., Fujihara, H., Katoh, A., Otsubo, H., Ishikura, T., Ueta, Y.
  • Journal Title: Neuroscience Letters 484(1) Pages: 26-29
  • Peer Reviewed
• [Journal Article] Acid-sensing ion channels in rat hypothalamic vasopressin neurons of the supraoptic nucleus. (2010)
  • Author(s): Ohbuchi, T., Sato, K., Suzuki, H., Okada, Y., Dayanithi, G., Murphy, D., Ueta, Y.
  • Journal Title: Journal of Physiology (London) 588(12) Pages: 2147-2162
  • Peer Reviewed
• [Journal Article] Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus soerules. (2010)
  • Author(s): Todoroki, M., Ueta, Y., Fujihara, H., Otsubo, H., Shibata, M., Hashimoto, H., Kobayashi, M., Sakamoto, H., Kawata, M., Dayanithi, G., Murphy, D., Hiro, H., Takahashi, K., Nagata, S.
  • Journal Title: Stress 13(4) Pages: 281-291
  • Peer Reviewed
• [Journal Article] Centrally administered relaxin-3 induces Fos expression in the osmosensitive areas in rat brain and facilitates water intake. (2010)
  • Author(s): Otsubo, H., Onaka, T., Suzuki, H., Katoh, A., Ohbuchi, T., Todoroki, M., Kobayashi, M., Fujihara, H., Yokoyama, T., Matsumoto, T., Ueta, Y.
  • Journal Title: Peptides 31(6) Pages: 1124-1130
  • Peer Reviewed
• [Journal Article] Electrophysiological identification of the functional presunaptic nerve terminal on an isolated single vasopressin neurone of the rat supraoptic nucleus. (2010)
  • Author(s): Ohbuchi, T., Yokoyama, T., Fujihara, H., Suzuki, H., Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology 22(5) Pages: 413-419
  • Peer Reviewed
• [Journal Article] TRPV1 gene deficiency attenuates miniature EPSCs potentiation induced by mannitol and angiotensin II in supraoptic magnocellular neurons. (2010)
  • Author(s): Yokoyama, T., Saito, T., Ohbuchi, T., Hashimoto, H., Suzuki, H., Otsubo, H., Fujihara, H., Nagatomo, T., Ueta, Y.
  • Journal Title: Journal of Neuroscience 30(3) Pages: 876-884
  • Peer Reviewed
• [Journal Article] Centrally administered ghrelin potently inhibits water intake induced by angiotensin II and hypovolemia in rats. (2010)
  • Author(s): Hashimoto, H., Otsubo, H., Fujihara, H., Suzuki, H., Ohbuchi, T., Yokoyama, T., Takei, Y., Ueta, Y.
  • Journal Title: The Journal of Physiological Sciences 60(1) Pages: 19-25
  • Peer Reviewed
• [Journal Article] Robust upregulation of nuclear red fluorescent-tagged Fos marks neuronal activation in green fluorescent vasopressin neurons after osmotic stimulation in a double transgenic rat. (2009)
  • Author(s): Fujihara, H., Ueta, Y., Suzuki, H., Katoh, A., Ohbuchi, T., Otsubo, H., Dayanithi, G., Murphy, D.
  • Journal Title: Endocrinology 150(12) Pages: 5633-5638
  • Peer Reviewed
• [Journal Article] Ghrelin potentiates miniature excitatory postsynaptic currents in rat supraoptic magnocellular neurons. (2009)
  • Author(s): Yokoyama, T., Saito, T., Ohbuchi, T., Suzuki, H., Otsubo, H., Okamoto, T., Fujihara, H., Nagatomo, T., Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology(Cover article of the issue) 21(11) Pages: 183-190
  • Peer Reviewed
• [Journal Article] Exaggerated response of a vasopressin-enhanced green fluorescent protein transgene to nociceptive stimulation in the rat. (2009)
  • Author(s): Suzuki, H., Kawasaki, M., Ohnishi, H., Otsubo, H., Ohbuchi, T., Katoh, A., Hashimoto, H., Yokoyama, T., Fujihara, H., Dayanithi, G., Murphy, D., Nakamura, T., Ueta, Y.
  • Journal Title: Journal of Neuroscience 29(42) Pages: 13182-13189
  • Peer Reviewed
• [Journal Article] Response of arginine vasopressin-enhanced green fluorescent protein fusion gene in the hypothalamus of adjuvant-induced arthritic rats. (2009)
  • Author(s): Suzuki, H., Onaka, T., Kasai, M., Kawasaki, M., Ohnishi, H., Otsubo, H., Saito, T., Hashimoto, H., Yokoyama, T., Fujihara, H., Dayanithi, G., Murphy, D., Nakamura, T., Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology 21(3) Pages: 183-190
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus. (2009)
  • Author(s): Ohbuchi, T., Yokoyama, T., Saito, T., Hashimoto, H., Suzuki, H., Otsubo, H., Fujihara, H., Suzuki, H., Ueta, Y.
  • Journal Title: Brain Research 1258(3) Pages: 34-42
  • Peer Reviewed
• [Journal Article] Chronic osmotic stimuli increase salusin-β-like immunoreactivity in the rat hypothalamo-neurohypophyseal system : possible involvement of salusin-β on [Ca^<2+>]_i increase and neurohypophyseal hormone release from the axon terminals. (2008)
  • Author(s): Saito, T., Dayanithi, G., Saito, J., Onaka, T., Urabe, T., Watanabe, TX., Hashimoto, H., Yokoyama, T., Fujihara, H., Yokota, A., Nishizawa, S., Hirata, Y., Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology 20(2) Pages: 207-219
  • Peer Reviewed
• [Journal Article] Induction of the galanin-like peptide gene expression in the posterior pituitary gland after acute osmotic stimulus in rats. (2007)
  • Author(s): Kawasaki, M., Saito, J., Hashimoto, H., Suzuki, H., Otsubo, H., Fujihara, H., Ohnishi, H., Nakamura, T., Ueta, Y.
  • Journal Title: Neuroscience Letters 419(2) Pages: 125-130
  • Peer Reviewed
• [Journal Article] Centrally and peripherally administered ghrelin potently inhibits water intake in rats. (2007)
  • Author(s): Hashimoto, H., Fujihara, H., Kawasaki, M., Saito, T., Shibata, M., Otsubo, H., Takei, Y., Ueta, Y.
  • Journal Title: Endocrinology 148(4) Pages: 1638-1647
  • Peer Reviewed
• [Journal Article] Physiological studies of stress responses in the hypothalamus of vasopressin-enhanced green fluorescent protein transgenic rat. (2007)
  • Author(s): Shibata, M., Fujjihara, H., Suzuki, H., Ozawa, H., Kawata, M., Dayanithi, G., Murphy, D., Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology 19(4) Pages: 285-292
  • Peer Reviewed
• [Journal Article] Exaggerated response of arginine vasopressin-enhanced green fluorescent protein fusion gene to salt loading without disturbance of body fluid homeostasis in rats. (2006)
  • Author(s): Fujio, T., Fujihara, H., Shibata, M., Yamada, S., Onaka, T., Tanaka, K., Morita, H., Dayanithi, G., Kawata, M., Murphy, D., Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology(Cover article of the issue) 18(10) Pages: 776-785
  • Peer Reviewed
• [Presentation] Regulation of stress responses by peptides : New transgenic animal models. (2010)
  • Author(s): Ueta, Y.
  • Organizer: The 7^<th> International Congress of Neuroendocrinology symposium
  • Place of Presentation: Rouen, France
  • Year and Date: 20100711-20100715
• [Presentation] The expression of the vasopressin-enhanced green fluorescent protein fusion gene in the hypothalamo-pituitary system in rats : a new aspect of vasopressin dynamics. (2010)
  • Author(s): Ueta Y., Fujihara, H., Murphy, D.
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20100326-20100330
• [Presentation] 浸透圧センシングとバゾプレッシン分泌 (2010)
  • Author(s): 上田陽一
  • Organizer: 特定領域研究公開シンポジウム「細胞感覚:セルセンサーのモーダルシフト」
  • Place of Presentation: 東京
  • Year and Date: 2010-12-23
• [Presentation] TRPチャンネルから視た神経分泌ニューロンの物理・化学感受性 (2009)
  • Author(s): 上田陽一、横山徹
  • Organizer: 第39回日本臨床神経生理学会学術大会シンポジウム
  • Place of Presentation: 北九州
  • Year and Date: 20091118-20091120
• [Presentation] 多色蛍光タンパクを発現するトランスジェニックラットの作出と浸透圧刺激に対する反応 (2009)
  • Author(s): 藤原広明、鈴木仁士、加藤明子、大坪広樹、上田陽一
  • Organizer: 第50回日本組織細胞化学会
  • Place of Presentation: 滋賀
  • Year and Date: 20090926-20090927
• [Presentation] 視索上核大細胞性神経分泌細胞の浸透圧感受性におけるTRPV1の関与 (2009)
  • Author(s): 横山徹、大淵豊明、藤原広明、長友敏寿、上田陽一
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090916-20090918
• [Presentation] 中枢性浸透圧調節とTRPチャネル (2008)
  • Author(s): 上田陽一
  • Organizer: 第61回日本自律神経学会総会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 20081106-20081107
• [Presentation] ラット視索上核大細胞性神経分泌ニューロンへのシナプス入力におけるTRPA1の役割:電気生理学的検討 (2008)
  • Author(s): 横山徹、大淵豊明、藤原広明、長友敏寿、上田陽一
  • Organizer: 第35回日本神経内分泌学会
  • Place of Presentation: 東京
  • Year and Date: 20080828-20080830
• [Presentation] 浸透圧センサーとバゾプレッシン分泌 (2008)
  • Author(s): 上田陽一
  • Organizer: 第26回内分泌代謝学サマーセミナー
  • Place of Presentation: 名古屋
  • Year and Date: 20080711-20080712
• [Presentation] 神経分泌細胞における浸透圧およびペプチドセンシングメカニズム (2008)
  • Author(s): 上田陽一、横山徹
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 20080709-20080711
• [Presentation] TRPチャネルと中枢性浸透圧調節 (2008)
  • Author(s): 上田陽一
  • Organizer: 第81回日本内分泌学会学術総会シンポジウム
  • Place of Presentation: 青森
  • Year and Date: 20080516-20080518
• [Presentation] ラット視索上核大細胞性神経分泌ニューロンへの興奮性シナプス入力に対するグレリンの効果 (2008)
  • Author(s): 横山徹、齋藤健、大淵豊明、橋本弘史、鈴木仁士、大坪広樹、藤原広明、長友敏寿、上田陽一
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 20080325-20080327
• [Presentation] 遺伝子改変動物による下垂体後葉ホルモン研究の新展開 (2008)
  • Author(s): 上田陽一
  • Organizer: 第85回日本生理学会大会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20080325-20080327
• [Presentation] ストレスにおけるラット視床下部-下垂体後葉系のバゾプレッシンeGFP融合遺伝子発現と病態生理学的意義 (2008)
  • Author(s): 上田陽一、鈴木仁士
  • Organizer: 第17回日本病態生理学会大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 20080126-20080127
• [Presentation] ラットにおける中枢性体液調節:ストレス・ペプチド・浸透圧感受性 (2008)
  • Author(s): 上田陽一
  • Organizer: 第33回日本比較内分泌学会大会シンポジウム
  • Place of Presentation: 広島
  • Year and Date: 2008-12-05
• [Presentation] バゾプレッシンとオキシトシン;ストレスと生殖の視点から (2007)
  • Author(s): 上田陽一
  • Organizer: 第100回日本繁殖生物学会大会、第12回日本生殖内分泌学会学術集会合同大会ジョイントシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20071019-20071022
• [Presentation] The expression of the arginine vasopressin-enhanced green fluorescent protein fusion gene in the transgenic rat brain. (2007)
  • Author(s): Ueta, Y., Matsui, A., Fujihara, H., Sakamoto, F., Kawata, M., Dayanithi, G., Murphy, D.
  • Organizer: VIIth World Congress on Neurohypophysial Hormones
  • Place of Presentation: Regensburg, Germany
  • Year and Date: 20070918-20070922
• [Presentation] 新規循環関連ペプチドと下垂体後葉系 (2006)
  • Author(s): 上田陽一
  • Organizer: 第29回日本神経科学大会シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 20060719-20060721
• [Presentation] バゾプレッシンニューロン研究の今:遺伝子改変動物を用いた研究 (2006)
  • Author(s): 上田陽一
  • Organizer: 第16回神経科学の基礎と臨床
  • Place of Presentation: 大阪
  • Year and Date: 2006-12-16
• [Remarks]
  • URL: http://www.nips.ac.jp/cellsensor/NL16.html